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AntagomiR-27a Targets FOXO3a in Glioblastoma and Suppresses U87 Cell Growth in Vitro and in Vivo
Ge, Yun-Fei,Sun, Jun,Jin, Chun-Jie,Cao, Bo-Qiang,Jiang, Zhi-Feng,Shao, Jun-Fei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2
Objective: To study the effect of the antagomiR-27a inhibitor on glioblastoma cells. Methods: The miR-27a expression level in specimens of human glioblastoma and normal human brain tissues excised during decompression for traumatic brain injury was assessed using qRT-PCR; The predicted target gene of miR-27a was screened out through bioinformatics databases, and the predicted gene was verified using genetic report assays; the effect of antagomiR-27a on the invasion and proliferation of glioma cells was analyzed using MTT assays and 5-ethynyl-2'-deoxyuridine (EdU) labeling. A xenograft glioblastoma model in BALB-c nude mice was established to detect the effect of antagomiR-27a on tumour growth. Results: qRT-PCR results showed that miR-27a significantly increased in specimens from glioblastoma comparing with normal human brain tissues. Th miR-27a inhibitor significantly suppressed invasion and proliferation of glioblastoma cells. FOXO3a was verified as a new target of miR-27a by Western blotting and reporter analyzes. Tumor growth in vivo was suppressed by administration of the miR-27a inhibitor. Conclusion: MiR-27a may be up-regulated in human glioblastoma, and antagomiR-27a could inhibit the proliferation and invasion ability of glioblastoma cells.
Jie Qi,Xiaojiang Li,Xin Lv,Yang Ge,Jinyu Wang,Hongsheng Lu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.110 No.-
Wax precipitation leads to destructive harms in the exploitation and transportation of petroleum. Solventtreatments can remove paraffin wax easily but the separation of solvent relying on distillation is admittedinefficient. Aiming at enhancing the separation and recovery performance of paraffin wax, CO2-responsive emulsion (SHS/W emulsion) based on switchable hydrophilicity solvents (SHS) is adopted. Since the paraffin wax is dissolved, emulsified, and dispersed by SHS/W emulsion, SHS/W emulsion exhibitsbetter removal performance than SHS. The paraffin wax is recovered effectively upon introducingCO2, which attributes to the transformation of SHS from hydrophobic to hydrophilic state. Comparedwith the SHS system, the recovery of paraffin wax is improved obviously by introducing surfactantSpan80. Owing to the deprotonation of SHS by bubbling N2 and heating, 77.6% SHS is recycled after recoveringparaffin wax. Here, the recycled SHS can be used to realize the removal of paraffin wax again. Thus,it is a promising and alternative strategy for realizing the sustainable and rapid removal of paraffin wax inthe petroleum industry.
Ge Guo,Jie Xiao,Byoung Ryong Jeong 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10
Korean fir (Abies koreana E. H. Wilson) is a unique Pinaceae tree species endemic in Korea. The population of A. koreana has been declining, probably due to the climate change, and now it is one of the rare plant species that needs to be protected and conserved. In this study, callus induction was studied to find out the best combination of plant growth regulators (PGRs) using post-dormancy winter buds as the explants. In January 2021, after a winter dormancy period, buds were collected from about 10 year-old mature tree. The winter buds removed of rind after surface disinfection were transferred to the Murashige and Skoog (MS) medium supplemented with different combinations of auxins [2,4-dichlorophenoxyacetic acid (2,4-D) and α-naphthaleneacetic acid (NAA)] and cytokinins [6-benzylaminopurine (6-BA), kinetin (KT) and thidiazurone (TDZ)]. These explants planted in Petri dishes were cultured in a culture room with a day/night temperature of 24/18°C and 80% relative humidity in the dark. After 4 weeks, treatments of combinations of PGRs showed significant effects on callus induction ratio, callus browning ratio, and fresh weight of callus. Among the 13 treatments, the highest and lowest callus induction ratio was 96.3% in the 1.0 mg·L<SUP>-1</SUP> 2,4-D + 0.2 mg·L<SUP>-1</SUP> TDZ + 2.0 mg·L<SUP>-1</SUP> 6-BA and 66.1% in the control MS medium without supplementation of any PGR, respectively. The lowest browning ratio of callus was 3.7% in the 1.0 mg·L<SUP>-1</SUP> 2,4-D + 0.2 mg·L<SUP>-1</SUP> TDZ + 2.0 mg·L<SUP>-1</SUP> 6-BA, and the greatest callus fresh weight of 985.8 mg was observed in the 1.0 mg·L<SUP>-1</SUP> 2,4-D + 2.0 mg·L<SUP>-1</SUP> NAA + 1.0 mg·L<SUP>-1</SUP> KT. In a conclusion, the 2,4-D consistently exerted a positive influence on callus induction from post-dormancy winter buds throughout this experiment.
Jie Zhang,Ben Dong,Ying Han,Xiaocui Zhan,Sijie Ge,Shilong He 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.3
In this paper, Cobalt-doped α-MnO2 (i.e., Co-α-MnO2) were synthesized through hydrothermal method. Phenol was employed as targeted pollutants to investigate the catalytic ozonation performance of Co-α-MnO2. Results showed that Co-α-MnO2 significantly improved the phenol removal increased to 97.47 % after 40 min, which was 16.46 %, 38.92 % higher than that of α-MnO2 catalytic ozonation and single ozonation without catalyst. Additionally, the physicochemical properties of α-MnO2 and Co-α-MnO2 were analyzed using technologies such as XRD, TEM, BET and XPS. Compared to α-MnO2, Co-α-MnO2 has larger specific surface area (79.496 m2/g) and pore volume (0.0396 cm3/g), higher Mn3+ relative content (41.16 %) and adsorbed oxygen content (18.99 %). Also, the oxygen vacancy content, lattice defect content and surface hydroxyl content of Co-α-MnO2 are higher than that of α-MnO2, which could result in higher catalytic oxidation performance of Co-α-MnO2. The influence of masking agent showed that surface hydroxyl group, •OH and •O2− were involved in the catalytic ozonation of phenol. This study could help recognize the role of surface hydroxyl groups and active free radicals and demonstrate the contribution of reactive oxygen species on phenol removal in Co-α-MnO2 systems.
Iron Source and Medium pH Affect Growth and Development of Hydrangea macrophylla In Vitro
Jie Xiao,Ge Guo,Yali Li,Byoung Ryong Jeong 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.5
Hydrangea macrophylla is a popular perennial ornamental shrub grown commercially as potted plants, landscape plants, and cut flowers. The aim of this work was to investigate effect of iron (Fe) source and medium pH on growth and development of H. macrophylla in vitro. The Fe sources used, including non-chelated iron sulfate (FeSO4) and iron ethylenediaminetetraacetic acid (Fe-EDTA), were supplemented to the multipurpose medium with a final Fe concentration of 2.78 mg·L<sup>-1</sup>. The medium without any supplementation of Fe was used as the control. The pH of the agar-solidified medium was adjusted to either 4.70, 5.70, or 6.70 before autoclaving. The experiment was conducted in a culture room for 60 days at 25/18°C day and night temperatures, and a 16hour photoperiod provided at a light intensity of 50 mmol·m<sup>-2</sup>·s<sup>-1</sup> photosynthetic photon flux density (PPFD) from white light emitting diodes. Both Fe source and pH affected growth and development of the micro propagated plantlets in vitro. The leaf color was greener in the pH 4.70 treatment regardless of the Fe source. Besides, both Fe sources increased the leaf chlorophyll content. Numbers of shoots and roots in plantlets treated with FeSO4 were 2.0 and 2.7 times greater than those of the control, respectively. Fresh and dry weights of the shoot and the root were the greatest in the treatment of FeSO4 combined with pH 4.70. The tissue Fe content decreased with the increasing medium pH, and contents of potassium (K), calcium (Ca), and magnesium (Mg) in the plantlets increased in the pH 4.70 treatment regardless of the Fe source. Overall, the plantlet absorbed more Fe in the pH 4.70, and FeSO4 combined with pH 4.70 was found to be the best for growth and development of H. macrophylla plantlets in vitro.
Jie Zhang,Ben Dong,Ding Ding,Shilong He,Sijie Ge 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.5
In this paper, MnO₂ catalyst were firstly prepared and modified by four kinds of anionic precursors (i.e., NO₃⁻, AC⁻, SO₄<SUP>2-</SUP> and Cl-) through redox precipitation method. After that, bio-treated coking wastewater (BTCW) was prepared and employed as targeted pollutants to investigate the catalytic ozonation performance of prepared-MnO₂ catalyst was investigated and characterized by the removal efficiencies and mechanism of the prepared bio-treated coking wastewater (BTCW), which was employed as the targeted pollutants. Specifically, the effects of specific surface area, crystal structure, valence state of Mn element and lattice oxygen content on catalytic activity of MnO₂ materials were characterized by BET, XRD and XPS, respectively. Results showed that COD of BTCW could be removed 47.39% under MnO₂-NO₃⁻ catalyst with 2 h reaction time, which was much higher than that of MnO₂-AC⁻ (3.94%), MnO₂-SO₄<SUP>2-</SUP> (12.42%), MnO₂-Cl⁻ (12.94%) and pure O₃ without catalyst (21.51%), respectively. So, MnO₂-NO₃⁻ presented the highest catalytic performance among these catalysts. The reason may be attributed to a series of better physiochemical properties including the smaller average grain, the larger specific surface area and active groups, more crystal defect and oxygen vacancy, higher relative content of Mn<SUP>3+</SUP> and adsorbed oxygen (Oads) than that of another three catalysts.