시상부위에 발생한 단일성 뇌농양 : 증례 보고 및 문헌고찰 Report of Two Cases and Review of the Literature

곽호신,김동규,이상형,정희원,이현구,김현집,한대희 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.12

The authors present two cases of solitary thalamic abscess which were intially diagnosed as malignant thalamic glioma. After treatment with steroids and osmotic agents, the patients' clinical conditions improved after two to three days. However, sudden deterioration of consciousness level developed and the CT scan showed acute hydrocephalus with increment of thalamic low-density lesions. We performed extraventricular drainage and obtained CSF containing numerous inflammatory cells. This suggested the possibility that the lesion was a pyogenic abscess. which progressed to ventriculitis, rather than a malignant glioma. Stereotactic aspiration of the lesion confirmed thalamic abscess. These findings and review of previous reports of thalamic abscess suggested that early diagnosis and stereotactic drainage of the thalamic abscess is of primary importance.

C₄H_9O_6N(Ammonium Hydrogen D-Tartrate)의결정 구조

김문집,박해윤,박호종 순천향대학교 기초과학연구소 2001 순천향자연과학연구 논문집 Vol.7 No.1

The structure of Ammonium Hydrogen D-Tartrate(C4H9O6N) has been determined by X-ray diffraction methods. The crystals are Orthorhombic, space group P212121, unit cell constants, a=7.6431(7)Å, b=7.8418(5)Å, c= 11.0605(11)Å, α= β= γ=90˚, V=662.92(10)Å3, T= 296(2)K, Z=4, Dc= 1.6744Mgm-3. The intensity data were collected on an Enarf-Noius CAD-4 Diffractometer with graphite monochromated Mo Kα radiation. The molecular structure was solved by direct method and refined by full-matrix least squares to a final R = 3.18% for 682 unique observed F0>4σ(F0) reflections and 137 parameters. The molecular have 8 intermolecular hydrogen bonds.

Mutation in Ret Proto - Oncogene in Medullary Thyroid Cancer and Multiple Endocrine Neoplasia Type 2A ( Men 2A ) and Men 2B with Hirschcprung`s Disease

유순집(Soon Jib Yoo),이희진(Hee Jin Lee),안유배(Yu Bae Ahn),한제호(Je Ho Han),윤건호(Kun Ho Yoon),강무일(Moo Il Kang),차봉연(Bong Yun Cha),이광우(Kwang Woo Lee),손호영(Ho Young Son),강성구(Sung Koo Kang),김영실(Young Sill Kim),김병기(By 대한내과학회 1998 대한내과학회 추계학술대회 Vol.55 No.-

성인에서 자기 공명 영상을 이용한 슬개골 두께에 대한 통계학적 분석

김명호(Myung-Ho Kim),유문집(Moon-Jib Yoo),서중배(Joong-Bae Seo),박희곤(Hee-Gon Park),심상호(Shang-Ho Shim) 대한정형외과학회 2005 대한정형외과학회지 Vol.40 No.6

목적: 자기 공명 영상을 이용하여 측정한 슬개골의 두께를 분석하여 우리 나라 성인의 슬개골 두께에 대한 기초적인 자료를 얻는 것과 동시에, 슬관절 전치환술시 슬개골 치환물 선택에 도움이 될 자료로 제시하기 위한 목적으로 본 연구를 시행하였다. 대상 및 방법: 2002년 10월부터 2004년 6월까지 시행한 20세에서 39세 사이(평균 29세)의 성인 남녀 165예의 슬관절 자기 공명 영상을 이용하였다. 슬개골의 골절 또는 각종 변형이 있는 경우는 제외하였으며, 대상에 포함된 슬관절은, 반월상 연골 손상이 54예, 십자 인대 손상이 53예, 슬부 좌상이 24예, 정상 슬관절 17예, 기타 17예 등이었다. 계측 방법은 슬부 자기 공명 영상의 축상면 영상과 시상면 영상에서 슬개골의 전방 피질골에서 관절면의 연골까지 가장 두꺼운 부분의 두께를 0.1 ㎜ 단위로 측정하여 평균치를 구하였으며, 남녀간의 차이 및 체중과 신장에 대한 연관성도 분석하였다. 결과: 자기 공명 영상으로 측정한 슬개골 두께의 평균은 축상면 영상에서 남자는 23.8 (SD 1.4) ㎜, 여자에서 21.7 (SD 1.4) ㎜이었다. 축상면 영상에서 측정한 것과 시상면 영상에서 측정한 수치의 차이는 없었으며, 남성의 슬개골 두께가 여성보다 의미 있게 두꺼웠으며(p 〈0.01), 성인의 신장이 클수록, 체중이 무거울수록 슬개골 두께도 두꺼운 것으로 분석되었다(p〈0.01). 결론: 한국인의 평균 슬개골 두께는 남자가 23.8 ㎜, 여자가 21.7 ㎜로서, 여자보다는 남자가 두꺼웠고, 신장이 클수록, 몸무게가 무거울수록 슬개골이 더 두꺼웠다. Purpose: To provide information on the thickness of the patella in Korean adults and to provide a standard for the selection of a proper patella implant in total knee arthroplasty. Materials and Methods: From October 2002 to June 2004, the patellar thickness was measured by MRI in 165 knees. The mean age of the patients was 29 years (20-39 years). Patients with either patellar fractures or deformities were excluded. Of the knees included in this study, 54 knees had meniscal injuries, 53 had cruciate ligament injuries, 24 had knee contusions, and 17 had other disorders. The remaining 17 knees were normal. The measurements were taken in the axial and sagittal images of the MRI scans. The maximum distance in millimeter between the anterior cortex of the patella and the surface of the articular cartilage was measured to the first decimal place. The relationship between the patellar thickness and gender, weight and height was also analyzed. Results: The average patellar thickness on the axial image was 23.8 (SD 1.4) ㎜ in males and 21.7 (SO 1.4) ㎜ in females. The measurement estimated in the axial image was not significantly different from that in the sagittal image. The patellar thickness in males was significantly thicker than in females (p<0.01). There was a significant correlation between the patellar thickness and both the body weight and height (p<0.01). Conclusion: The average patellar thickness in Korean adults was 23.8 ㎜ in males and 21.7 ㎜ in females. The patella was thicker in males than in females. Moreover, the patellar thickness was proportional to the height and weight of each individual.

소의 대동맥 내피세포에서 산화 저밀도지단백이 인슐린 결합 및 인슐린수용체의 내재화와 재순환에 미치는 영향

김혜수,송기호,강무일,윤건호,유순집,장상아,안유배,문성대,이광우,차봉연,한제호,손호영,허갑법 대한당뇨병학회 1999 Diabetes and Metabolism Journal Vol.23 No.3

Background: Endothelial dysfunction is perhaps one of the earliest manifestations of atherosclerosis. This abnormality is in part due to altered membrane signal transduction in endothelial cells. Oxidized LDL that is atherogenic may induce endothelial dysfunction, and its presence has been documented in atherosclerotic vessels. Many studies have shown that oxidized LDL inhibits signaling pathways mediated by inhibitory GTP-binding proteins (Gi- protein). It is also known that G-protein is involved in insulin recycling on cultured human umbilical vein endothelial cells. Therefore, to determine the effect of oxidized LDL on endothelial cells: insulin binding, internalization, and the recycling of insulin receptors were assessed in cultured bovine aortic endothelial cells treated with native LDL, oxidized LDL, and in some cells pretreated with pertussis toxin before the incubation with oxidized LDL. Method: Native LDL (density 1.019 1.063 g/mL) was obtained from using the rapid single discontinuous density gradient ultracentrifugation of plasma samples from a single donor. Oxidized LDL was prepared by exposing samples of native LDL to CuSO4 (5 uM) at 37't for 24 hours. Endothelial cells at 80% confluence were treated with the indicated concentrations of native LDL, oxidized LDL, and some cells were pretreated with pertussis toxin for 6 hrs before the incubation with oxidized LDL. These cells were incubated for 24 72 hours. Results: 1. Binding of (125)I-insulin(0.17nM) to endothelial cells treated with increasing concen- trations of oxidized LDL shows dose-dependent decrease. There were significant differences in insulin binding between native LDL and oxidized LDL-treated cells (p$lt;0.05). Binding of 'I-insulin (0.17 nM) to endothelial cells treated with increasing culture time of oxidized LDL shows more decreased than that of native LDL significantly (p$lt;0.05). And oxidized LDL had additive effect, but not significant, with pertussis toxin on the specific (125)I-insulin binding to bovine aortic endothelial cells. 2. Internalization of insulin receptors reached rapidly to its maximal level around 30min at 37'C. At 60 min, oxidized-LDL treated cells was less increased in internalization of insulin receptors than that of native LDL treated cells [59.1+1.9% of total cell associated insulin (mean+SE) vs. 67.5+1.1%, p$lt;0.05]. There were additive effects, but not significant differences, between oxidized LDL and pretreated with pertussis toxin before the incubation with oxidized LDL. 3. After 30 min of incubation with unlabeled insulin (33 nM), insulin binding in oxidized LDL treated cells was significantly higher compared to native LDL treated cells (69.0+2.5% of control values vs. 63.7+1.2%, p$lt;0.05), suggesting that oxidized-LDL decreased internalization of insulin receptors. And during the process of recycling, there were significant differences in insulin receptor recycling between the oxidized LDL and native LDL treated cells, but oxidized LDL had an additive effect, but not significant, with pertussis toxin on insulin receptor recycling to the bovine aortic endothelial cells. Conclusion: 1. The findings in this study suggest that oxidized LDL may play a causative role to produce the insulin resistance by inhibiting insulin binding, internalization and recycling of insulin receptor in cultured bovine aortic endothelial cells 2. This study suggests that the effect of oxidized LDL to the bovine aortic endothelial cells in insulin binding and receptor-mediated transcytosis is caused by inhibiting pertussis toxin sensitive Gi-protein. $quot;

Evaluation of forage yield and quality for wild soybean accessions and progenies derived from a cross Glycine soja × G. max

Eun-Ja Lee,Hong-Jib Choi,Dong-Hyun Shin,Chan-Ho Kwon,J. Grover Shannon,Jeong-Dong Lee 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Soybean is desirable as a forage crop because of it has high protein and oil concentration. Wild soybean, a progenitor of cultivated soybean, has a softer stem and higher protein content in seed than cultivated soybean. There is little information on yield and forage quality for wild soybean and its derivatives. The objective of this study was to determine the forage yield and quality of wild soybeans and selected soybeans derived from a cross G. max ×G. soja. Forage yield and quality were assessed for three grain soybean cultivars, three wild soybeans and three selected lines from G. max×G. soja. Forage quality attributes such as crude protein (CP), crude fat (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF), digestible dry matter (DDM), dry matter intake (DMI) and relative feed value (RFV) were determined at the R2, R4 and R6 developmental stages. Forage yield and CF were highest at stage R6 in G. max, G. soja and selected G. max×G. soja lines. CP content was similar between R2 and R4 but increased sharply after R4 and peaked at R6 in G. max and selected lines from G. soja×G. max. On the other hand, CP content was similar between R4 and R6 stage in wild soybeans. Generally, NDF and ADF were highest at stage R4 but decreased at stage R6. DDM, DMI, and RFV increased between R4 and R6. These results suggest that R6 was the optimal harvest stage to provide forage of highest quality and yield. A study was conducted in 2011 to evaluate forage yield and quality at stage R6 in 25 lines from PI483463 (G. soja)×Hutcheson (G. max) and four cultivated grain soybeans. Hutcheson had the highest forage yield with 24.7t/ha infresh weight (FW) among grain soybeans. Line W11 had the highest forage yield(25.7t/ha,FW) among G. soja×G. max selections and four other lines had similar forage yield compared to Hutcheson. Generally the 25 lines from this G. max×G. soja cross had thinner main stems and branches than cultivated soybeans. When the 25 lines were evaluated for their feed quality as per forage grade by AFGC, nine lines rated prime grade and all 25 lines were classified as forage Grade 1. Results of this study indicate crosses between wild and cultivated soybean show promise for improving soybean as a forage crop.

제2형 당뇨병 동물모델인 OLETF 쥐에서 장기간 라미프릴 투여가 내당능 및 췌도 베타세포에 미치는 효과

고승현,윤건호,김명미,안유배,송기호,유순집,손현식,차봉연,이광우,손호영,강성구 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:최근 소개된 HOPE(Heart Outcomes Prevention Evaluation)study의 결과에 의하면, 심혈관질환이나 이에 대한 위험인자가 1가지 이상인 9,541명을 대상으로 안지오텐신 전환효소 억제제의 일종인 라미프릴을 투여하여 사망율과 뇌졸증, 심부전, 당뇨병 합병증이 개선되었을 뿐 아니라 다른 항고혈압제에 비해 새롭게 당뇨병으로 진단되는 환자가 줄었음을 보고한바 있다. 이에 저자들은 라미프릴(ramipril)을 제2형 당뇨병 동물모델에 장기간 투여함으로써 당뇨병을 예방하거나 고혈당 상태 또는 인슐린 감수성을 개선시킬수 있는지를 알아보고자 하였다. 방법:생후 24주된 체중 400∼450g의 OLETF와 LETO 쥐를 대상으로 6개월간 라미프릴 경구투여 하였고 혈압, 24시간 단백뇨 및 인슐린 내성검사를 시행하였다. 6개월 이후로는 8주간 투여군과 대조군의 체중을 비슷하게 맞추어 내당능 상태를 평가하였고 이후 다시 8주간 고농도 포도당을 투여하였다. 경구당부하검사를 시행한 후 희생시켜 조직표본을 만들어 guinea pig anti­human insulin 항체로 염색후 DAB로 발색하여 point count 법으로 베타세포를 정량하였고, 면역염색으로 췌도의 변화와 섬유화의 정도를 관찰하였다. 결과:라미프릴을 6개월간 투여하였을 때 OLETF 쥐에서 1)체중 증가가 적었으며 2)경구 당부하검사시 포도당하 면적은 의미있게 감소하였고 3)인슐린 내성검사상 Kitt가 증가되는 경향을 보였으며 4)수축기, 이완기 혈압과 24시간 단백뇨양이 의미있게 감소하였고 5)체중을 맞추면 내당능 상태가 비슷해졌다가 고농도 포도당 주입시 다시 포도당하 면적이 대조군에서 더 증가되었다. 라미프릴 투여군에서 췌도변형 및 파괴와 탈과립, 췌도내 섬유화가 감소하였다. 결론:본 실험에서는 인슐린 비의존성 당뇨병 동물 모델인 OLETF 쥐에서 당뇨병 발생 이전 단계부터 장기간 라미프릴을 투여할 경우 체중증가가 적었고, 투여군과 대조군의 체중을 맞추었을 때는 내당능상태의 차이가 없아가 고혈당 투여시 다시 투여군과 대조군에서 의미있는 차이를 보였다. 라미프릴 투여가 베타세포의 기능면에서 이로운 효과를 보일 것으로 생각되었다. 따라서 비만한 환자에서 안지오텐신 전환효소 억제제의 장기투여는 단백뇨 감소, 혈압강하 효과 이외에 체중증가의 억제 및 당뇨병 발생을 억제할 가능성을 시사한다. Background : In a Heart Outcomes Prevention Evaluation HOPE study, ramipril, a long-acting angiotensin-converting enzyme (ACE) inhibitor, significantly reduced the death rates the number of myocardial infarctions, strokes, heart failure as well as the risk of complications related to diabetes and of diabetes itself. However, it is known that ACE inhibitors improve glucose tolerance or insulin sensitivity or reduce the incidence of diabetes. Methods : 24 week-old OLETF (Otsuka Long Evans Tokushima Fatty) rats weighing 400 ~ 450 g were used in this study. 4 groups of rats were examined in parallel for 40 weeks. The OLETF rats were randomized for treatment with an aqueous solution of ramipril (5 mg/Kg) daily [OL (RMP), n=10)] and with saline [OL (CON), n=10)]. The LETO rats were also randomized in the same was as the OLETF rats (LT(RMP), n=10, LT (CON), n=10). The blood glucose level, body weight, systolic and diastolic blood pressure was assessed every month. At 3 and 6 months, the 24hrs urinary protein concentration was measured, and as insulin tolerance test and oral glucose tolerance test were conducted in all experimental groups. After 6 months, the body·weight was matched for 2 months in each corresponding group. Subsequently, a 15% sucrose loading was done for 2 months. After the glucose tolerance test, the pancreas was excised and immuno histochemical staining was conducted for insulin to quantify the beta cell mass by a point-counting method. In addition, the islet morphology was evaluated in the pancreas. Results : Ramipril treatment for a period of 6 months improved the 2hr blood glucose level, the area under the glucose curve in the oral glucose tolerance test, insulin sensitivity in addition to lowering significantly systolic and diastolic blood pressure and 24hrs urinary protein level significantly in OLETF rats. Of note, a lower weight gain was observed in both the ramipril-treated animals at 6 months. After weight matching, the AUC g and 2hr blood glucose level values were similar between the corresponding groups, but a 15% sucrose loading worsened the AUC g value. Histologically, the islets were less disorganized and the extent of fibrosis was lower in the ramipril-treated OLETF rats in the trichrome stain. Conclusion : Long-term treatment of ramipril, a long acting angiotensin-converting enzyme inhibitor may be useful for suppressing weight gain and proteinuria in addition to having a protective effect on the islet to harmful stimuli such as hyperglycemia (J Kor Diabetes Asso 25:469~482, 2001).

한국인 당뇨병성 대혈관합병증 환자에서의 Methylenetetrahydrofolate Reductase 유전자 다형성

이광우,송기호,손호영,강무일,윤건호,유순집,강성구,안유배,오기원,이원영,차붕연 대한당뇨병학회 1999 Diabetes and Metabolism Journal Vol.23 No.5

조혈모세포이식술이 단기간의 골대사에 미치는 영향

이광우,송기호,손호영,강무일,윤건호,유순집,강성구,장상아,안유배,문성대,오기원,김춘추,최윤희,김혜수,차봉연 대한내분비학회 1999 Endocrinology and metabolism Vol.14 No.2

Background: The organ transplantation becomes the management of choice for many patients with chronic and life threatening heart, liver, kidney, bone marrow, and pancreatic diseases. A new set of side effects unique to this groups of patients has become recognized. Bone disease is one of these complications. It is well known that there is an interplay between the cells in the bone marrow and the surrounding bone tissue. Marrow stromal cells include the progenitors of the osteoblastic lineage are the sources of effector molecules that support and regulate both hematopoiesis and bone remodeling. But little is known about the effects of myeloablative treatment followed by bone marrow transplantation(BMT) on bone metabolism. Methods: We have investigated prospectively in 29 patients undergoing BMT(4 autologous, 25 allogenic) for hematologic diseases(19 leukemia, 9 severe aplastic anemia, 1 myelodyspoietic syndrome). Serum concentrations of calcium, phosphorus, creatinine, gonadotropins, sex hormones and biochemical markers of bone turnover(osteocalcin and carboxyterminal cross-linked telopeptide of type I collagen(ICTP)] were measured. The samples were collected before BMT and 1, 2, 3, 4, 12 weeks, 6 months and 1 year thereafter. Bone mineral density was measured with DEXA(Dual Energy X-ray Absorptiometry) before and after 1 year of BMT. Results: 1. ICIP was progressively increased until 4 weeks after BMT when peak values were reached. And then decreased thereafter and basal values were regained after 1 year. Osteocalcin was progressively decreased until 3 weeks after BMT when nadir values were reached. And then increased thereafter and basal values were regained after 3 months. No distinct differences were observed in serum biochemical turnover marker between both sexes and between patients who received total body irradiation and those who did not. 2. Lumbar BMD was 2.1% decreased from 1.113 +- 0.132 g/cm to 1.089 +- 0.137 g/cm, and femoral BMD was 6.2% decreased fiom 1.078 +- 0.156 g/cm to 1.011 +- 0.157 g/cm. 3. 92% of the women (11/12) became menopausal manifested by high gonadotropin and low estradiol levels immediately after BMT. In contrast to women, gonadotropins and testosterone levels were not changed significantly in men after BMT. Conclusion: The rapid impairment of bone formation and also increase in bone resorption, as mirrored by the biochemical markers in this study, might play a role for the post-BMT bone loss. Further studies over many patients with a longer follow up will be needed. (J Kor Soc Endocrinol 14:355-364, 1999)

정상 혈압을 보이는 인슐린비의존형 당뇨병환자의 24시간 혈압 변화에 대한 연구

이광우,송기호,손호영,손현식,강무일,한제호,윤건호,유순집,강성구,이종민,차봉연,박인수 대한당뇨병학회 1996 Diabetes and Metabolism Journal Vol.20 No.1