Effects of Free Anthraquinones Extract from the Rhubarb on Cell Proliferation and Accumulation of Extracellular Matrix in High Glucose Cultured-Mesangial Cells

Jianyun Wang,Hui Fang,Bingzheng Dong,Dongdong Wang,Yan Li,Xiao Chen,Lijuan Chen,Tong Wei,Qunli Wei 대한약리학회 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.6

Diabetic nephropathy (DN) is the leading cause of end-stage failure of the kidney, but the efficacy of currently available strategies for the prevention of DN remains unsatisfactory. In this study, we investigated the effects of free anthraquinones (FARs) extract, which was extracted from the rhubarb and purified by macroporous resin DM130 with gradient mixtures of ethanol/water as the lelution solvents, in high glucose-cultured glomerular mesangial cells (MCs). The cell proliferation was determined by CCK-8 assay, the levels of TGF-β1, CTGF, ColIV and FN proteins in the supernatant of MCs were measured by ELISA assays, and the mRNA levels of these four genes were detected by RT-PCR. The results showed that the increased proliferation of MCs, the mRNA levels and protein expression of TGF-β1, CTGF, ColIV and FN induced by high glucose were inhibited after the treatment with the FARs extract. This indicated that FARs extract could inhibit cell proliferation and the expression of main extracellular matrix induced by high glucose in MCs. The FARs extract exhibited potential values for prophylaxis and therapy of DN.

Numerical Simulation Analysis of Damage Mode of Concrete Gravity Dam under Close-in Explosion

Jianyun Chen,Xiaopeng Liu,Qiang Xu 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.1

The study of damage mode of a concrete gravity dam under close-in explosive impact is a critical issue to assess the dam’s antiknock security. In this paper, a fluid-structure coupled finite element numerical method with combination of Lagrangian and Eulerian algorithm is applied, taking into consideration the damage development process of the Huangdeng concrete gravity dam. The damage mode of a square reinforced concrete slab under close-in blast loads has been simulated and discussed as a comparison with real experiment to verify the feasibility of the numerical simulation. The influence of the presence of spillway tunnel on damage mode of the concrete gravity dam has been investigated. Different damage modes of the concrete gravity dam are compared and studied for different standoff distances and different detonation depths. The damage process of the dam with time course is researched. The influence of different mediums, where the explosion occurs, on the failure mode of the dam is also researched. The analysis results show that attention should be paid to the concrete gravity dam subjected to close-in upstream shallow underwater blasts. It also should be focused on that the presence of spillway tunnel can affect the damage result of the dam.

Study on the Influence of Variation of Contact Arc Zone on the Single-Pass Sawing of Sapphire Wafer

Jianyun Shen,Lang Lu,Yuanyuan Gong,Xipeng Xu 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.9

Single-pass sawing (SPS), a significant cutting process widely used in the manufacturing of optical components in optoelectronic fields, which can greatly improve the quality of products by reducing the proportion of edge chipping. In this paper, the mechanism of SPS was introduced by a novel approach of deep analysis of the variation of the contact arc zone. Setting sapphire wafers as the workpiece, this study performed the comparative experiments in three specific arc zones, which would further make a thorough inquiry on the related machining mechanism. The difficulty degree of machining and the topography of surface quality during SPS process were also analyzed. The difficulty degree of machining was reflected by the sawing force ratio, and the machining surfaces of sapphire wafers were compared by SEM and Digital Microscope. The results indicate that the lower the arc is, the easier it can be machined during SPS. And the best surface quality appears in Arc 2 (arc angle = 20°), which can be set as an excellent reference for the machining of other materials during SPS. The single-pass sawing mechanism is also derived on the basis of the traditional cutting mechanism model, which will further serve as useful references to both the industrial machining and the academia.

Seismic Damage Indexes of a High Arch Dam Based on the Monolith

Jianyun Chen,Lijun Qin,Qiang Xu,Jing Li 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.7

To assess safety of a dam in an earthquake, establishing the performance evaluation index of the arch dam is significant. The finite-element model based on the 289 m-high Baihetan Dam in Southwest China is taken as a case for seismic response analysis, in which the radiation damping of infinite foundation and opening of contraction joints are taken into consideration. Using the incremental dynamic analysis (IDA) approach, a three-component earthquake scaled to 12 increasing intensity levels is applied to the model, and the seismic response of the model is analysed. In this paper, it is proposed to study the damage based on the monolith, so the monolith damage volume ratio and monolith damage area ratio are suggested to determine the most severe part of the dam. Meanwhile, to help identifying the damage of the dam, the response results of contraction joints are extracted to analyse the potential relationship between the proposed indexes, providing a reference for exploring new detection indexes and damage evaluation indexes. In order to explore the possibility of the simplified layout of dam damage monitoring, the dam body is partitioned according to the results of monolith damage development.

Exploration on Damage Mechanism and Equivalent Damage Model of High Arch Dams under Earthquakes

Jianyun Chen,Xiangyu Cao,Qiang Xu,Jing Li 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.4

Damage identification technology of high arch dams has been well developed under strong earthquakes. However, the failure mechanism of high arch dams at different locations remains unclear. For this purpose, a series of deterministic dynamic analyses for a 3D 289-m-high arch dam based on concrete damaged plasticity constitutive model are performed. The failure mechanism at different locations of high arch dams under earthquakes is studied systematically. According to the sensitivity of different indicators to different damaged positions, an effective sensor placement scheme is proposed to assess the seismic reliability in practical engineering. Furthermore, a new effective equivalent damage model based on prior information is proposed, which can improve the efficiency of damage identification, as well as solving the problem that the real damage is concealed by homogenizing the damage in a pre-set region. The results provide suggestions and theoretical basis for sensor placement in actual monitoring of high arch dams; and the equivalent damage model provides a new solution to balance the efficiency and accuracy of damage identification.

Analysis of transcriptomic profiles and physiological traits of exogenous 24-epibrassinolide alleviating salt stress in Atractylodes macrocephala Koidz.

Zhang Jianyun,Fang Huiyong,Jiang Jianming,Gu Xian,Zhao Huimei,Qiu Jinmiao,Wang Qian,Zheng Yuguang,Zheng Kaiyan 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.1

Exogenous 24-epibrassinolide (24-epiBL) application is a preferred method for improving salt tolerance in plants. However, information about the effects of exogenous 24-epiBL application related to salt response in Atractylodes macrocephala Koidz. (AMK) has rarely been reported. Here, we aimed to investigate the effects of exogenous 24-epiBL (0.01 mg L−1) on enhancing salt tolerance in AMK seedlings under salt stress (5 g L−1 NaCl) based on seed germination experiments. Our results indicated that NaCl treatment suppresses AMK seed germination, while 24-epiBL treatment under nonstress and stress conditions had positive effects on plant seedling growth. Treatment with NaCl significantly inhibited radicle elongation and root activity to endure salt stress, and 24-epiBL treatment promoted growth and alleviated the damage to osmotic stress, ion stress sub- stances, and oxidative stress by regulating free proline (Pro) and soluble protein (SP) contents and enhancing soluble sugar (SS) conversion. Moreover, the expression of normal development process genes was affected, and differentially expressed genes (DEGs) in multiple pathways associated with amino acid metabolism, signal transduction, carbohydrate metabolism, and lipid metabolism were regulated under NaCl treatment. Further analysis of the AMK transcriptome profile revealed that exogenous 24-epiBL enhanced salt tolerance through hormone regulation and carbohydrate metabolism promotion, salt- responsive gene expression inhibition, and induction of pathways related to terpenoid and polyketide metabolism as well as pentose and glucuronate interconversions for plant growth promotion. Collectively, our findings successfully demonstrate that exogenous 24-epiBL plays a positive role in regulating the AMK response to salt stress, which provides potential genetic resources to improve salt tolerance in plants.

Effects of Free Anthraquinones Extract from the Rhubarb on Cell Proliferation and Accumulation of Extracellular Matrix in High Glucose Cultured-Mesangial Cells

Wang, Jianyun,Fang, Hui,Dong, Bingzheng,Wang, Dongdong,Li, Yan,Chen, Xiao,Chen, Lijuan,Wei, Tong,Wei, Qunli The Korean Society of Pharmacology 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.6

Diabetic nephropathy (DN) is the leading cause of end-stage failure of the kidney, but the efficacy of currently available strategies for the prevention of DN remains unsatisfactory. In this study, we investigated the effects of free anthraquinones (FARs) extract, which was extracted from the rhubarb and purified by macroporous resin DM130 with gradient mixtures of ethanol/water as the lelution solvents, in high glucose-cultured glomerular mesangial cells (MCs). The cell proliferation was determined by CCK-8 assay, the levels of TGF-${\beta}1$, CTGF, ColIV and FN proteins in the supernatant of MCs were measured by ELISA assays, and the mRNA levels of these four genes were detected by RT-PCR. The results showed that the increased proliferation of MCs, the mRNA levels and protein expression of TGF-${\beta}1$, CTGF, ColIV and FN induced by high glucose were inhibited after the treatment with the FARs extract. This indicated that FARs extract could inhibit cell proliferation and the expression of main extracellular matrix induced by high glucose in MCs. The FARs extract exhibited potential values for prophylaxis and therapy of DN.

Numerical-Simulation-Based Buffer Design for Microchip Electrophoresis with Capacitively Coupled Contactless Conductivity Detection

Meng Jianyun,AREGA NEBIYU GETACHEW,PULLAGURA BHARGAV KR,Kim Dohyun 한국바이오칩학회 2024 BioChip Journal Vol.18 No.1

We present a numerical simulation method for designing a buffer system in microchip electrophoresis (MCE) equipped with capacitively-coupled-contactless-conductivity detection (C4D). One of the key design considerations for MCE-C4D is background electrolyte (BGE). This is because a C4D typically exhibits low sensitivity, and optimizing BGE conditions (e.g., base and acid species, pH, and ionic strength) can improve its sensitivity. However, BGE has been traditionally designed through experience or trial and error, which is time- and reagent-consuming. In this study, we employ Simul 5, an open- source electrophoresis simulation software, for rational BGE design. Four BGEs including trimethylamine (TEA)/acetic acid (AcOH, pH 10.6), MES/His (pH 6.1), MES/TRIS (pH 8.1), and TRIS/HCl (pH 7.4), previously used in electrophoresis-C4D of amino acids and protein, were selected for evaluation of our numerical method. Glutamic acid (Glu) was selected as a model analyte for initial simulation verifi cation. Our numerical simulation revealed that the best achievable detection sensi- tivity was 1.046 × 10 –5 S/(m μM) in the TRIS/HCl buffer because anionic Glu species with a low mobility (27 × 10 –9 m 2/Vs) replaced Cl − co-ion of a high mobility (79.1 × 10 –9 m 2/Vs) in the analyte zone, leading to a signifi cant negative conductivity peak. TEA/AcOH, MES/His, and MES/TRIS buffers exhibited progressively lower sensitivity. After the initial evaluation, trypsin inhibitor (TI), a more complex proteinous analyte was tested in the MES/His and MES/TRIS BGEs. The best detec- tion sensitivity was 1.032 × 10 –4 S/(m μM) in the MES/TRIS buffer because counter-ionic species TRIS + of a high mobility (29.5 × 10 –9 m 2/Vs) was replaced by the ionic TI, characterized by a large charge (− 11.5) and a low mobility (8.08 × 10 –9  m2/ Vs), resulting in a strong negative peak. Based on a comprehensive analysis of the impacts of compositional changes in each ionic species of the analyte zone on conductivity-peak height, we propose a BGE design guideline for enhanced sensitivity. Subsequent MCE-C4D confirmation experiments demonstrated excellent qualitative agreement with the simulation results for the Glu and TI analytes. We anticipate that our numerical analysis method will find wide application in designing BGEs for portable MCE-C4D systems by enhancing sensitivity.

One-pot synthesis of electron-acceptor composite enables efficient fullerene-free ternary organic solar cells

Zhang, Jianyun,Liu, Wenrui,Chen, Shanshan,Xu, Shengjie,Yang, Changduk,Zhu, Xiaozhang The Royal Society of Chemistry 2018 Journal of Materials Chemistry A Vol.6 No.45

<P>An effective strategy of “synthesizing an electron-acceptor composite (ZITI-m) by one-pot reaction” to improve photovoltaic performance is proposed. The ZITI-m composite is synthesized by one-pot Knoevenagel reaction of ZIT-2CHO and a mixture of INCN-2F and I-a, from which asymmetrical ZITI-3F and symmetrical ZITI-4F are separated. J71:ZITI-m-based OSC shows the highest PCE of 13.65 ± 0.13% with remarkable PCE of 13.85% than those based on J71:ZITI-3F (12.97 ± 0.15%) and J71:ZITI-4F (13.02 ± 0.13%) blends. The effect of acceptor ratio in the composite on OPV performance indicates that all PCEs of composite system are higher than those of the materials based on pure acceptors with a broad composition tolerance, which is ascribed to the synergistic effect of the ternary blend system.</P>

謝靈運著作考略

杜偉偉(Jiang Jianyun),姜劍雲(Liu Jie) 한국교통대학교 동아시아연구소 2007 동아문헌연구 Vol.- No.-