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        Identifying natural genotypes of grain number per panicle in rice (Oryza sativa L.) by association mapping

        Jianyin Xie,Fengmei Li,Najeeb Ullah Khan,Xiaoyang Zhu,Xueqiang Wang,Zhifang Zhang,Xiaoqian Ma,Yan Zhao,Quan Zhang,Shuyang Zhang,Zhanying Zhang,Jinjie Li,Zichao Li,Hongliang Zhang 한국유전학회 2019 Genes & Genomics Vol.41 No.3

        Introduction As one of the main yield components, grain number per panicle (GNP) played critical role in the rice yield improvement. The identification of natural advantageous variations under different situations will promote the sustainable genetic improvement in rice yield. Objectives This study was designed to identify natural genotypes in a rice mini-core collection, to examine the genotypic effects across the indica and japonica genetic background in different environments, and excavating the superior genotypes that had drove the modern genetic improvement. Methods The association mapping of GNP was carried out using a mini-core collection including 154 indica and 119 japonica accessions in seven different environments. Genotypic effects of each genotype for each QTL were calculated and genotype frequency distortion between the commercial rice cultivars and landraces was screened by χ2-test. Results In total, 74 QTLs containing stable and sensitive QTLs in various environments were detected. Within them, 20 positive and 24 negative genotypes in indica, and 24 positive and 16 negative genotypes in japonica were identified. When checking the accumulation of positive genotypes identified in indica across cultivars in each of the two subspecies, it indicated that increased number of positive genotypes identified in indica results in the substantially increased GNP in both indica and japonica across all of the environments, while this trend was not obvious for the positive genotypes identified in japonica especially in short day environments. Moreover, the positive and negative genotype frequency distortion between the landraces and commercial rice cultivars indicated that both positive selection of positive genotypes and negative selection of negative genotypes had driven the genetic improvement on GNP. Conclusion Our findings suggested that the accumulation of positive genotypes and purifying negative genotypes played equivalently important roles in the improvement of rice yield, but the efficient use for some QTLs or genotypes depends on the comprehensive evaluation of their effect under diverse genetic backgrounds and environments.

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        Stability of Switched Positive Linear Systems with Actuator Saturation under Mode-dependent Average Dwell Time

        Lijie You,Jianyin Fang,Xiaowu Mu 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.4

        The stability problem is investigated for switched positive linear systems (SPLS) with unstabilized subsystem and actuator saturation. By using Lyapunov functions method and mode-dependent average dwell time (MDADT) strategy, sufficient conditions of the stability for the considered systems are established. We also give an estimate of the attraction domain. The convex hull represent approach is used to deal with actuator saturation. Finally, two examples are carried out to show the validity of results, which is less conservative than the average dwell time (ADT) switching.

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        Effect of reaction conditions on one-step preparation of 1H,1H,2H,2HPerfluorooctyltrimethoxysilane by catalytic hydrosilylation over RuCl33H2O catalysts

        Feihong Ye,Liping Li,Jianyin Xu,Lu Zhou 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.110 No.-

        Synthesization of 1H,1H,2H,2H-Perfluorooctyltrimethoxysilane through solvent-free hydrosilylation of1H,1H,2H-perfluoro-1-octene with trimethoxysilane was efficiently conducted over the RuCl33H2O catalyst. The synergistic action of Ru3+ as the active center and the strong electronegativity of Cl to attractthe electron of the electron-donating group –OCH3 in trimethoxysilane, activated the Si-H and thus promotedthe reaction. The influences of different reaction conditions including feed order, feed ratio, catalystdosage, reaction temperature and reaction time on the reaction yield were fully investigated, toconclude the optimized condition to achieve a yield of 86.80% was the feed of 1.2 mol trimethoxysilaneper mole of 1H,1H,2H-perfluoro-1-octene, together with the addition of 9.56 104 mol RuCl33H2O permole of trimethoxysilane, at 80 C for 4 h; while the additive of CuCl to RuCl33H2O catalyst could furtherimprove the reaction yield at 50℃. Finally, after understanding the activity of Ru with different valencestate, the reaction mechanism was also proposed.

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        Development and on-orbit operation of loop heat pipes on chinese circumlunar return and reentry spacecraft

        Zhang Hongxing,Mi Min,Miao Jianyin,Wang Lu,Chen Yang,Ding Ting,Ning Xianwen,Huo Yuhua 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.6

        This paper describes the development and result of ground experiment and analyzes the on-orbit operation of Loop heat pipes (LHP) for the Chinese circumlunar return and reentry spacecraft. A novel LHP was developed to satisfy the thermal control requirements of Inertial measurement units (IMU). The LHP consisted of an integrated evaporator subassembly, a cambered condenser, and an electric explosion valve. Ground experiments were conducted to investigate the startup characteristics, and heat transfer and heat insulation performance of the LHPs. During a flight mission, the LHPs work as a thermal switch and perform both heat transfer and insulation functions. Before returning to the earth’s atmosphere, the valves of the reentry capsule are opened to discharge working fluid and prevent heat transfer from the capsule wall to the devices. In the flight mission, the LHPs performed all the required functions, and the IMU temperatures were maintained within an allowable range.

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        Inhibition of Invasion and Metastasis of MHCC97H Cells by Expression of Snake Venom Cystatin through Reduction of Proteinases Activity and Epithelial-Mesenchymal Transition

        Nanhong Tang,Qun Xie,Xiaoqian Wang,Xiujin Li,Yanlin Chen,Xu Lin,Jianyin Lin 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.5

        Snake venom cystatin (sv-cystatin) is a member of the cystatin family of cysteine protease inhibitors. To further evaluate the possibility of sv-cystatin in cancer therapy, this study examined the effects of sv-cystatin on the invasion and metastasis of liver cancer cells (MHCC97H) in vitro and in vivo as well as the underlying mechanism. sv-cystatin cDNA was transfected into MHCC97H cells and the anti-invasion and antimetastasis effects of sv-cystatin were determined using migration and matrigel invasion assays and a lung-metastasis mice model. The results suggest that sv-cyst clone (sv-cystatin expression in MHCC97H cells) delayed the invasion and metastasis in vitro and in vivo compared to the parental, mock and si-sv-cyst clone cells (inhibited sv-cystatin expression by siRNA). The decreased activities of cathepsin B, MMP-2 and MMP-9 and EMT change index including higher E-cadherin, lower N-cadherin and decreased Twist activity were observed in the sv-cyst clone, which contributes to the change in invasion and metastasis ability of MHCC97H cells. This study provides evidence that expression of the sv-cystatin gene in MHCC97H cells inhibits tumor cell invasion and metastasis through the reduction of the proteinases activity and Epithelial-Mesenchymal Transition (EMT), which might contribute to the anticancer research of the sv-cystatin protein.

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