Effects of the Maceration Enzymes on Evolution of Pyranoanthocyanins and Cinnamic Acids During the Cabernet Gernischet (Vitis vinifera L. cv.) Red Wine Making

Jian-Jun He,Fu-Liang Han,Qing-Quan Yu,Qiu-Hong Pan,Chang-Qing Duan,Guo-Li Cheng 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.3

The effects of the maceration enzymes on the evolution of 4-vinylphenols pyranoanthocyanins and cinnamic acids were investigated during the Cabernet Gernischet red wine making. The results showed that the contents of p-coumaric acid and caffeic acid in the maceration enzyme-treated wine (the treated wine) were higher than those in the maceration enzyme-untreated wine (the control wine) at the alcoholic fermentation (AF),maceration (M), and malolactic fermention (MLF) stages. Malvidin-3-O-glucoside-4-vinylphenol (Mv-glu-vp) and malvidin-3-O-(6-O-acetyl)-glucoside-4-vinylphenol (Mvacet-glu-vp) were formed at the AF and M stages in the treated wine, while they were formed at the end of MLF stage in the control wine. However, the contents of these 2kinds of 4-vinylphenol pyranoanthocyanins in the control wine were higher than those in the treated wine during aging. These results showed that the use of maceration enzymes in winemaking can promote p-coumaric acid and p-caffeic acid transformed to vinylphenols and accelerate the formation of pyranoanthocyanins.

Study of the Synthesis and Properties of Ir(III) Complexes Based on Phosphine Carbonylimide Derivative as Auxiliary Ligand

Qing-Mei Guo,Guoli Huang,Xin-Ying Yin,Jian-Jian Bian,Xiao-Mei Li,Ming-Yu Teng 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.12

Three iridium complexes of (dfppy)2Ir(DAPB), (dfppy)2Ir(DAPB), and (dfppy)2Ir(DAPB) were synthesized by using phosphine carbonylimide derivative as auxiliary ligand and fluoro?/trifluoromethyl substituted 2-phenylpyridine derivatives as primary ligands. Their quantum efficiency range from 19.4% to 38.5%, and show strong rigidochromic effect. The maximum emission wavelengths of solid-state fluorescence have remarkable red shifts from 39 to 55?nm compared with these in CH2Cl2.

Disruption of ecdysis in Leptinotarsa decemlineata by knockdown of chitin deacetylase 1

Jian-Jian Wu,Li-Li Mu,Zhong-Chao Chen,Kai-Yun Fu,Wen-Chao Guo,Chao Li,Guo-Qing Li 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.2

Chitin deacetylases (CDAs) catalyze N-deacetylation of chitin, a crucial process for chitin modification. In the present paper, LdCDA1 was identified in Leptinotarsa decemlineata. It was copiously expressed in larval foregut, hindgut and epidermis. Just before the molt in the first, second and third larval instars, the mRNA levels of LdCDA1 were high. In the fourth (final)-instar larvae, a peak occurred 4 days after ecdysis. In vivo results revealed that LdCDA1 transcriptionally responded, positively and negatively respectively, to 20-hydroxyecdysone and juvenile hormone titers. Moreover, knockdown of LdCDA1 significantly reduced foliage consumption, lengthened developing period and prevented growth in the final instar larvae. Three distinct lethal phenotypes were noted in the LdCDA1 RNAi larvae. About 30% of the RNAi larvae became moribund and finally died; approximately 50% of deformed pupae died as pharate adults; and around 20% of LdCDA1 depleted pupae finally emerged as abnormal adults and eventually died within 1 week after emergence. Furthermore, chitin content was low and the mRNA levels of five chitin biosynthesis transcripts (LdUAP1, LdUAP2, LdChSAa, LdChSAb and LdChSB) were significantly declined in the LdCDA1 RNAi larvae. In addition, glucose, trehalose and glycogen contents were increased in the LdCDA1 depleted hypomorphs, along with highly expressed genes coding for trehalose and glycogen synthesis enzymes. The findings provide a compelling piece of evidence that CDA1 is critical for chitin deposition in L. decemlineata. Moreover, LdCDA1 may be a potential target for control of the larvae.

Flux Sliding-mode Observer Design for Sensorless Control of Dual Three-phase Interior Permanent Magnet Synchronous Motor

Jian-Qing Shen,Lei Yuan,Ming-Liang Chen,Zhen Xie 대한전기학회 2014 Journal of Electrical Engineering & Technology Vol.9 No.5

A novel equivalent flux sliding-mode observer (SMO) is proposed for dual three-phase interior permanent magnet synchronous motor (DT-IPMSM) drive system in this paper. The DTIPMSM has two sets of Y-connected stator three-phase windings spatially shifted by 30 electrical degrees. In this method, the sensorless drive system employs a flux SMO with soft phase-locked loop method for rotor speed and position estimation, not only are low-pass filter and phase compensation module eliminated, but also estimation accuracy is improved. Meanwhile, to get the regulator parameters of current control, the inner current loop is realized using a decoupling and diagonal internal model control algorithm. Experiment results of 2MW-level DT-IPMSM drives system show that the proposed method has good dynamic and static performances.

An Estimation Method of Consolidation and Settlement of Soil Mass in Dredger Fill Area Based on SBAS-InSAR

( Qing-bo Yu ),( Qing Wang ),( Xue-xin Yan ),( Tian-liang Yang ),( Meng Yao ),( Kai Zhou ),( Xin-lei Huang ),( Jian-ping Chen ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2

The land subsidence poses a nonnegligible threat to production and life, especially in dredger fill area. The essence of land subsidence is soil consolidation. Better evaluation of the degree of soil consolidation presents significant progress for predicting land subsidence. In this study, SBAS-InSAR was conducted to Chenjia town, the typical dredger fill area in Chongming East Shoal, Shanghai. SAR data and precise Orbit data were collected from the ESA satellite Sentinel-1 in recent 2 years with approximately one month intervals. the relationship curve of cumulative subsidence with time is obtained after processing. On this basis, the final settlement and soil consolidation can be estimated by applying the method proposed by Professor Huang Wenxi of China. The results show that: The subsidence rate is faster than 20 mm/a; The estimated final settlement is 26.6-52.9 mm, and the corresponding consolidation degree is 54.6 % on average; Soil mass has not yet been fully consolidated and it still takes a long time to achieve stability. In conclusion, the method proposed in this study can provide a clearer understanding of the consolidation degree, and a new reference for the evaluation and prevention of land subsidence. Future research will focus on longer monitoring time to enrich and improve the method. Acknowledgements This work was financially supported by National Nature Science Foundation of China (Grant No: 41820104001, 41430642 and 41627801) and the Key Laboratory of Land Subsidence Monitoring and Prevention, Ministry of Land and Resources (Grant No. KLLSMP201801).

Age of Diagnosis of Breast Cancer in China: Almost 10 Years Earlier than in the United States and the European Union

Song, Qing-Kun,Li, Jing,Huang, Rong,Fan, Jin-Hu,Zheng, Rong-Shou,Zhang, Bao-Ning,Zhang, Bin,Tang, Zhong-Hua,Xie, Xiao-Ming,Yang, Hong-Jian,He, Jian-Jun,Li, Hui,Li, Jia-Yuan,Qiao, You-Lin,Chen, Wan-Qin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: The study aimed to describe the age distribution of breast cancer diagnosis among Chinese females for comparison with the United States and the European Union, and provide evidence for the screening target population in China. Materials and Methods: Median age was estimated from hospital databases from 7 tertiary hospitals in China. Population-based data in China, United States and European Union was extracted from the National Central Cancer Registry, SEER program and GLOBOCAN 2008, respectively. Age-standardized distribution of breast cancer at diagnosis in the 3 areas was estimated based on the World Standard Population 2000. Results: The median age of breast cancer at diagnosis was around 50 in China, nearly 10 years earlier than United States and European Union. The diagnosis age in China did not vary between subgroups of calendar year, region and pathological characteristics. With adjustment for population structure, median age of breast cancer at diagnosis was 50~54 in China, but 55~59 in United States and European Union. Conclusions: The median diagnosis age of female breast cancer is much earlier in China than in the United States and the European Union pointing to racial differences in genetics and lifestyle. Screening programs should start at an earlier age for Chinese women and age disparities between Chinese and Western women warrant further studies.

Ram semen preserved at 0℃ with soybean lecithin Tris-based extender substituted for egg yolk

Zhao, Jian-qing,Xiao, Guo-liang,Zhu, Wen-liang,Fang, Di,Li, Na,Han, Chun-mei,Gao, Qing-hua Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.2

Objective: The present study evaluated the preservation of ram semen at 0℃ using soybean lecithin with a Tris-fructose extender. Methods: Semen was collected by artificial vagina ejaculation from six rams with proven fertility. High quality ejaculates were diluted by soybean lecithin (0.25%, 0.5%, 0.75%, 1.0%, 1.25%) using Tris-fructose extender and control (Tris-fructose egg yolk extender), respectively. The ejaculates were diluted to a concentration of 5×108 sperm/mL, followed by cooling to 0℃ in 90 min and maintaining the temperature for 12 days. The diluted semen samples were examined and recorded for sperm progressive motility, acrosome integrity at 0, 24, 72, 144, 216, 288 h, respectively. Two hundred and twenty-three ewes were inseminated for 216 h with optimal soybean lecithin concentrated semen or control via trans-cervical insemination. Results: The results showed that there were no differences in sperm progressive motility at 0, 24, 72, and 144 h (p>0.05). After 216 h, the sperm progressive motility in the control group and 0.5% concentration groups was significantly higher when compared to 0.25% concentration (p<0.05). The 0.5% concentration group demonstrated the highest survival rate and had no difference with the control group (p>0.05). At 216 h, the sperm progressive motility of all groups was still above 50%. The acrosome integrity of all groups was decreased with prolongation of storage time, but there was no difference at each time point (p>0.05). There was no significant difference in the lambing rate and pregnancy rate between the 0.5% concentration group and the control group (p>0.05). Conclusion: These results suggest that ram sperm is capable of fertilization after preservation at 0℃ with 0.5% of soybean lecithin in Tris-based extender substituted for egg yolk and produce normal offspring after insemination.

Neutrophil Count and the Inflammation-based Glasgow Prognostic Score Predict Survival in Patients with Advanced Gastric Cancer Receiving First-line Chemotherapy

Li, Qing-Qing,Lu, Zhi-Hao,Yang, Li,Lu, Ming,Zhang, Xiao-Tian,Li, Jian,Zhou, Jun,Wang, Xi-Cheng,Gong, Ji-Fang,Gao, Jing,Li, Jie,Li, Yan,Shen, Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Purpose: To explore the value of systemic inflammatory markers as independent prognostic factors and the extent these markers improve prognostic classification for patients with inoperable advanced or metastatic gastric cancer (GC) receiving palliative chemotherapy. Methods: We studied the prognostic value of systemic inflammatory factors such as circulating white blood cell count and its components as well as that combined to form inflammation-based prognostic scores (Glasgow Prognostic Score (GPS), Neutrophil-Lymphocyte Ratio (NLR), Platelet Lymphocyte Ratio (PLR), Prognostic Index (PI) and Prognostic Nutritional Index (PNI)) in 384 patients with inoperable advanced or metastatic gastric cancer (GC) receiving first-line chemotherapy. Univariate and multivariate analyses were performed to examine the impact of inflammatory markers on overall survival (OS). Results: Univariate analysis revealed that an elevated white blood cell, neutrophil and/or platelet count, a decreased lymphocyte count, a low serum albumin concentration, and high CRP concentration, as well as elevated NLR/PLR, GPS, PI, PNI were significant predictors of shorter OS. Multivariate analysis demonstrated that only elevated neutrophil count (HR 3.696, p=0.003) and higher GPS (HR 1.621, p=0.01) were independent predictors of poor OS. Conclusion: This study demonstrated elevated pretreatment neutrophil count and high GPS to be independent predictors of shorter OS in inoperable advanced or metastatic GC patients treated with first-line chemotherapy. Upon validation of these data in independent studies, stratification of patients using these markers in future clinical trials is recommended.

First-principles calculations on ferroelectricity and lattice dynamics of Type-II multiferroic SmMn2O5

Dai Jian-Qing,Yuan Jin 한국물리학회 2021 Current Applied Physics Vol.29 No.-

In this work, we report on the structural, electronic, and ferroelectric properties of SmMn2O5 by using firstprinciples density functional theory plus on-site Coulomb interaction (DFT + U) calculations. A thorough analysis was preformed to reveal the competing characteristics of different high-temperature (T) phases and the polarization mechanism in the low-T multiferroic phase. We show that the structural characteristics of the high-T phases have a strong influence on the low-T multiferroicity. In addition to the spin-induced lattice distortion that reduces substantially the purely electronic ferroelectricity, the dominant polarization mechanism in low-T SmMn2O5 still originates from the electronic polarization. By performing mode decomposition of the Hellmann– Feynman forces and the lattice distortion induced by the q = (0.5, 0, 0) magnetic order, we find that the Raman-active Ag mode characterized by the Mn4+O6 octahedron distortion and synergistic displacement of Mn3+ and Sm ions is of primary importance, while the infrared (IR)-active B2u mode plays a secondary role. These findings provide a theoretical foundation for future studies concerning the enhanced magnetoelectric effects of SmMn2O5 due to its pure exchange–striction mechanism.

The Relationship between HIF-2α and VEGF with Radiographic Severity in the Primary Osteoarthritic Knee

Zhou Jian-lin,Fang Hong-song,Peng Hao,Deng Shuang,Chen Shen,Li Jian-ping,Qiu Bo,Weng Jin-qing,Liu Feng 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.3

Purpose: The aim of this study was to determine the relationship of hypoxia-inducible factor-2 (HIF-2α) and vascular endothelial growth factor (VEGF) with radiographic severity in primary osteoarthritis (OA) of the knee. Expression of these two factors in cartilagesamples from OA knee joints was examined at mRNA and protein levels. Materials and Methods: Knee joints were examined using plain radiographs, and OA severity was assessed using the Kellgren and Lawrence (KL) grading system. Specimens were collected from 29 patients (31 knees) who underwent total knee replacementbecause of severe medial OA of the knee (KL grades 3 and 4), 16 patients who underwent knee arthroscopy (KL grade 2), and 5 patients with traumatic knees (KL grade 0). HIF-2α and VEGF expression was quantified by real-time polymerase chain reactionand western blotting. Results: Cartilage degeneration correlated with the radiographic severity grade. OA severity, determined using the Mankin scale, correlated positively with the KL grade (r=0.8790, p<0.01), and HIF-2α and VEGF levels with the radiographic severity of knee OA (r=0.7001, p<0.05; r=0.6647, p<0.05). Conclusion: In OA cartilage, HIF-2α and VEGF mRNA and protein levels were significantly and positively correlated. The expressionof both factors correlated positively with the KL grade. HIF-2α and VEGF, therefore, may serve as biochemical markers as well as potential therapeutic targets in knee OA.