DNA methylation and mRNA expression of COL6A3 in antler mesenchyme of female and male reindeer

Jian‑Cheng Zhai,Ruo‑Bing Han,Sheng‑Nan Wang,Qiang‑Hui Wang,Yan‑Ling Xia,Wei‑Shi Liu,Ya‑Jie Yin,He‑Ping Li 한국유전학회 2019 Genes & Genomics Vol.41 No.9

Backgroud Reindeer is the only deer species that both male and female produce antlers, which provides a particularly interesting case in studying the differences between antlers of the two sexes. Alpha 3(VI) Collagen Gene (COL6A3), forms a microfibrillar network associated with the structural integrity and biomechanical properties, has been found to be one of the differentially expressed genes in antler mesenchyme of female and male reindeer. Objective and Methods The promoter sequence of reindeer COL6A3 gene was obtained using the cloning technology and analyzed by the bioinformatics methods. Bisulfite sequencing PCR (BSP) was used to detect the methylation status of the COL6A3 promoter in reindeer antler mesenchyme. Real-time quantitative PCR was used to detect COL6A3 expression in the antler mesenchyme of female and male reindeer. Results Sequence analysis revealed that the reindeer COL6A3 partial promoter sequence was 983 bp including the possible promoter region at + 105 bp to + 155 bp. Homology and phylogenetic analysis indicated that the COL6A3 promoter of reindeer had the closest genetic distance with Bos taurus, Capra hircus and Ovis aries. BSP results indicated that the methylation level of COL6A3 promoter in the female reindeer antler mesenchyme was significantly higher than in the male. Correlating with increased methylation status, we also found that COL6A3 mRNA expression in female reindeer antler mesenchyme was significantly lower than in the male. Conclusion The higher methylation level of the COL6A3 gene in female reindeer antler mesenchyme coincides with decreased COL6A3 mRNA expression, thereby affecting the transposon silencing mechanism and possibly contributing to apparent differences of antlers in female and male reindeer.

Normal family of meromorphic functions

Jian-Ping Wang 대한수학회 2014 대한수학회보 Vol.51 No.3

We study normality for families of meromorphic functions which is related to an extended version of a Hayman's conjecture on value distribution, and prove several normality criteria for meromorphic functions and certain non-homogeneous differential polynomials.

Implicitization of rational curves and polynomial surfaces

Jian-ping Yu,Yong-li Sun 대한수학회 2007 대한수학회보 Vol.44 No.1

SOME APPLICATIONS OF q-DIFFERENTIAL OPERATOR

Jian-Ping Fang 대한수학회 2010 대한수학회지 Vol.47 No.2

In this paper, we use q-differential operator to recover the finite Heine 2Φ1 transformations given in [3]. Applying that, we also obtain some terminating q-series transformation formulas.

Apple RING finger E3 ubiquitin ligase MdMIEL1 negatively regulates salt and oxidative stresses tolerance

Jian-Ping An,Xin Liu,Lai-Qing Song,Chun-Xiang You,Xiao-Fei Wang,Yu-Jin Hao 한국식물학회 2017 Journal of Plant Biology Vol.60 No.2

RING-finger-containing E3 ubiquitin ligases play important roles in plant response to biotic and abiotoc stresses. In this study, through homology analysis, a Malus× domestica MYB30-Interacting E3 Ligase 1 gene, MdMIEL1, was identified and subsequently cloned from apple ‘Gala’ (Malus×domestica). MdMIEL1 contained a zinc finger domain close to N-terminus and a RING finger domain close to Cterminus. Expression of MdMIEL1 was significantly induced by NaCl and H2O2 treatments. Further study demonstrated that the MdMIEL1-overexpressing Arabidopsis and apple calli were less tolerance to salt stress than wild-type control. In addition, transgenic plants had higher levels of reactive oxygen species (ROS) (H2O2 and O2 –). And transgenic Arabidopsis and apple calli exhibited more sensitive phenotype to H2O2 treatment, which was associated with increased levels of ROS. These findings indicate MdMIEL1 is an important regulator involved in plant response to salt and oxidative stresses tolerance.

Explicit Finite Element Formulations for the Free Vibrations and Buckling of Partial-Interaction Composite Beams with Two Sub-components

Jian-Ping Lin,Bowen Chen,Xingxi Liu,Rongqiao Xu,Guannan Wang 한국강구조학회 2023 International Journal of Steel Structures Vol.23 No.4

The variational principles and corresponding finite element formulations are developed in this work for the free vibration and buckling analysis of partial-interaction composite beams under complex engineering situations, such as axial loading conditions, non-uniform material distributions, multi-span boundaries. The composites structures consist of sub-components connected with shear connections of finite rigidities and thus suffer from the partial-interaction phenomenon, significantly affecting the structural performance. The macroscopic elements for dynamic and buckling behavior are explicitly derived by simultaneously considering the sub-elements’ deformations and interlayer slips, avoiding the stress concentrations in the contact areas existing in the commercial packages. The generated frequencies and critical loads of several orders for the single- or two-span composite structures with different boundary conditions are then validated against the analytical and numerical results in the literature with well-matched agreement. The interfacial shear connections and structural parameters are also studied of their influences on the structural dynamic and buckling behavior. The present theory is finally implemented in the theoretical analysis of the steel box-girder viaduct with non-uniformly-distributed shear braces and multi-span continuous composite beams of Jiubao Bridge. The explicit expressions of the elements are provided and can be readily implemented into the commercial software with high computational efficiency.

Ethosomes, Binary Ethosomes and Transfersomes of Terbinafine Hydrochloride: A Comparative Study

Jian-Ping Zhang,Xin-An Wu,Yu-Hui Wei,Yan Zhou,Yu-Qing Li 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.1

The aim of this study was to compare the skin permeation of ethosomes, binary ethosomes and transfersomes of Terbinafine Hydrochloride (TH) under non-occlusive conditions. These lipid vesicles were prepared and characterized for shape, size, zeta-potential and entrapment efficiency. Franz diffusion cells and confocal laser scanning microscopy (CLSM) were used for the percutaneous absorption studies. The quantity of drug in the skin from ethosomes, binary ethosomes (the weight ratio of ethanol to propylene glycol 7:3, ethanol-PG = 7:3, w/w), and transfersomes was 1.26, 1.51 (p <0.05), 1.56 (p <0.01) times higher than that of TH from traditional liposomes (control). The skin deposition of the applied dose (DD%) of TH from ethosomes, binary ethosomes, and transfersomes was 3.34 (p < 0.05), 9.88 (p < 0.01), 2.52 times higher than that of TH from control. The results of CLSM experiments showed that penetration depth and fluorescence intensity of Rhodamine B from binary ethosomes was much greater than that from ethosomes and transfersomes. These results indicated the binary ethosomes (ethanol-PG = 7:3, w/w) most effectively permitted drug penetration through skin; transfersomes made drug easiest to accumulate in the skin. Ethosomes improved drug delivery with greater improvement in skin permeation than improvement in skin deposition.

Vector Passive Harmonic Mode-locking Fiber Laser Based on Topological Insulator Bi2Se3 Interacting with Fiber Taper

Jian Ping Li 한국광학회 2016 Current Optics and Photonics Vol.20 No.1

I propose a vector passive harmonic mode-locked fiber laser based on topological insulator Bi2Se3interacting with a fiber taper with a diameter of 7 μm. The particles of topological insulator are depositeduniformly onto the fiber taper with light pressure effect. By incorporating the fabricated saturable absorberinto an Er-doped fiber laser cavity, stable mode-locked fiber is obtained. Due to the intense evanescentfield of the fiber taper, strong confinement of light enhances the nonlinearity of the laser cavity, and passiveharmonic mode-locking is performed. I observe a maximum harmonic mode-locking of 356th, correspondingto a frequency of 3.57 GHz. The pulse duration is 824 fs, and the full width at half maximum of thespectrum is about 8.2 nm. The polarization dependent loss of the saturable absorber is ~ 2.5 dB in thewavelength range of the C band. As the cavity contains no other polarization dependent device, themode-locked laser is functioning in the vector state. The harmonic order vs pump power is investigated. To the best of our knowledge, this report is the highest frequency mode-locked fiber laser based on Bi2Se3. Experimental results indicate that the topological insulator Bi2Se3 functioning with a thin fiber taper iseffective for vector harmonic mode-locking.

The Real Estate Enterprise Supply Chain Logistics Model Research

Jian-ping You 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.12

To overcome the deficiencies of the existing literatures to describe the function of recovery quantity, it was necessary to construct a new function, in which recovery quantity connected with buy-back price and sale quantity. This paper analyzed the optimal decision of the manufacturer and competing retailers under the following three cases: two retailers participated in the recycle, one retailer participated in the recycle and the manufacturer collected directly. The results show that: when the saving cost (cost between new products and reused products) is large enough, the manufacturer tends to collect used products in the consumer market through the competing retailers. And when the saving cost is small, the manufacturer collects herself or chooses one of the competing retailers to collect the used products, which depends on the market size, the attract coefficient and the switching coefficient. Moreover, the improvement of the consumers’ environmental awareness is beneficial to the manufacturer, consumers and social environment. The results of this paper provide a basis for the manufacturer on its channel selection.

High alcohol-soluble MoOx gel for interfacial layer in organic solar cells

Jian Xiong,Zhen He,Shiping Zhan,Bingchu Yang,Xiaowen Zhang,Ping Cai,Cong Xu,Xiaogang Xue,Jian Zhang 한국물리학회 2017 Current Applied Physics Vol.17 No.8

Water-free solvent soluble, low-temperature processed metal oxides are important for preparing efficient and stable electronic devices, as well as the convenience in simplifying the device production process. Here we reported a facile approach with the features of low-temperature and solution-based process for the formation of a MoOx (s-MoOx) film as interface layer in polymer solar cells (PSCs). The absorbability, elementary composition, electronic property and surface microstructure of the s-MoOx are investigated in detail by ultravioletevisible spectrophotometer (UVevis), X-ray photoelectron spectrometry (XPS), ultraviolet photo-electron spectrometer (UPS) and atomic force microscopy (AFM). These investigations confirmed that such MoOx xerogel has high solubility in the organic alcohol solvents, such as ethanol and methanol. Meanwhile, this s-MoOx can be applied as the interfacial layer in organic solar cells via a low-temperature treatment (about 100 C) due to its proper physical properties, and a power conversion efficiency (PCE) over 3% was achieved. In addition, the devices with s-MoOx shows excellent air-stability, and the PCE efficiency can maintain about 84% of its initial value after 100 h exposure in air, which is dramatically enhanced comparing with the common devices with PEDOT:PSS layer.