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Yi‑Chia Kuan,Venkatesan Thiruvengadam,Jia‑Shin Lin,Jia‑Hsin Liu,Tsan‑Jan Chen,Hsin‑Mao Wu,Wen‑Ching Wang,Liang‑Jwu Chen 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.1
The broad-specificity amino acid racemase (Bsar) from Pseudomonas putida catalyzes the racemization of various amino acids, offering a flexible and feasible platform to develop a new non-antibiotic selectable marker system for plant transformation. In the present study, we demonstrated that a Bsar variant, Bsar-R174K, that is useful as a selectable marker gene in Arabidopsis and rice that were susceptible to l-lysine and D-alanine. The introduction of wild-type Bsar, Bsar-R174K or Bsar-R174A into E. coli lysine or asparagine auxotrophs was able to rescue the growth of these microorganisms in minimal media supplemented with selectable amino acid enantiomers. The transformation of Arabidopsis with Bsar or Bsar variants based on d-alanine selection revealed that Bsar-R174K had the greatest efficiency (2.40%), superior to kanamycin selectionbased transformation (1.10%). Whereas, l-lysine-based selection exhibited lower efficiency for Bsar-R174K (0.17%). The progenies of selected Bsar-R174K transgenic Arabidopsis revealed normal growth properties. In addition, Bsar-R174K transgenic rice was obtained on l-lysine medium with an efficiency of 0.9%, and the progenies of the transgenic rice revealed morphologically normal phenotypes comparable with their wild-type counterparts. This study presents the first report of broad range amino acid racemase Bsar-R174K as a non-antibiotic selectable marker system applied in transgenic plants.
An integrated dendrite-free zinc metal electrode for corrosion inhibition in aqueous system
Yi-Fan Hu,Li-Feng Zhou,He Gong,He Jia,Peng Chen,Yi-Song Wang,Li-Ying Liu,Tao Du 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.9
Zinc ion batteries have gotten increasing attention as a potential candidate for lithium-ion batteries, due totheir high specific capacity (820 mAh·g1), energy density, and safety. Inevitably, dendrite and corrosion create sometrouble for this system. Herein, an integrated Zn electrode coated by Zn-Al metal oxides prepared by a simple spincoatingmethod was utilized to increase the rechargeability for aqueous zinc ion batteries. By coating the Zn anodewith an artificial electrolyte interface, the wettability of Zn anodes was improved and impedance was reduced. Thecoating suppressed not only the appearance of dendrite but also the formation of corrosion products. The symmetricalcells with coating have a low overpotential (43mV) and an excellent life span. Meanwhile, the applied full batteriesexhibit an improved capacity retention rate (86.67% after 120 cycles), great rate performance and low apparent activationenergy (24.6 KJ·mol1). The simple production methods and superior corrosion suppression effects provide newideas for the anode protection of aqueous system batteries.
Microwave Assisted Synthesis of Graphene-Bi2MoO6 Nanocomposite as Sono-Photocatalyst
Jia-Yao Tang,Lei Zhu,Jia-Yi Fan,Chen-Sun,Won-Chun Oh 한국재료학회 2022 한국재료학회지 Vol.32 No.1
In this investigation, Bi2MoO6 deposited graphene nanocomposite (BMG) was synthesized using a simple microwave assisted hydrothermal synthesis method. The synthesized BMG nanocomposite was characterized by X-ray diffraction, transmission electron microscopy, scanning electron microscopy with energy dispersive X-ray analysis, and photocurrent analysis. The study revealed that the catalysts prepared have high crystalline nature, enhanced light responsive property, high catalytic activity, and good stability. XRD results of BMG composite exhibit a koechlinite phase of Bi2MoO6. The surface property is shown by SEM and TEM, which confirmed a homogenous composition in the bulk particles of Bi2MoO6 and nanosheets of graphene. The catalytic behavior was investigated by the decomposition of Rhodamine B as a standard dye. The results exhibit excellent yields of product derivatives at mild conditions under ultrasonic/visible light-medium. Approximately 1.6-times-enhanced sono-photocatalytic activity was observed by introduction of Bi2MoO6 on graphene nanosheet compared with control sample P25 during 50 min test.
Antimycobacterial Activity of Fusaric Acid from a Mangrove Endophyte and its Metal Complexes
Jia-Hui Pan,Yi Chen,Yu-Hong Huang,Yi-Wen Tao,Jun Wang,Yan Li,Yi Peng,Tao Dong,Xiao-Min Lai,Yong-Cheng Lin 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7
Due to the increasing prevalence of multidrug-resistant Mycobacterium tuberculosis, there is an urgent need for new antituberculosis drugs that have novel mechanisms of action. As part of our ongoing search for antimycobacterial metabolites from mangrove endophytes, chemical analysis of the active extract of a strain of Fusarium sp. was performed, which led to the isolation of fusaric acid as the predominant constituent. A variety of metal complexes of fusaric acid were prepared. Antimycobacterial assays showed that Cadmium (II) and Copper (II) complexes exhibited potent inhibitory activity against the M. bovis BCG strain [minimum inhibitory concentration (MIC) = 4 μg/mL] and the M. tuberculosis H37Rv strain (MIC = 10 μg/mL), respectively. This is the first report of the antimycobacterial activity of the mangrove Fusarium metabolite and its coordinating metal complexes.
Jia-Yi Yeh 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.13 No.1
The axisymmetric dynamic instability of polar orthotropic sandwich annular plate combinedwith electrorheological (ER) fluid core layer and constraining layer are studied in this paper. And, the ERcore layer and constraining layer are used to improve the stability of the annular plate system. Theboundaries of instability regions for the polar orthotropic sandwich annular plate system are obtained bydiscrete layer annular finite element and the harmonic balance method. The rheological property of anelectrorheological material, such as viscosity, plasticity, and elasticity can be controlled by applying differentelectric field strength. Thus, the damping characteristics of the sandwich system are more effective when theelectric field is applied on the sandwich structure. Additionally, variations of the instability regions for thepolar orthotropic sandwich annular plate with different applying electric field strength, thickness of ER layerand some designed parameters are investigated and discussed in this study.
Jia-Yi Yeh 국제구조공학회 2011 Smart Structures and Systems, An International Jou Vol.8 No.5
The parametric resonance problems of axisymmetric sandwich annular plate with an electrorheological (ER) fluid core and constraining layer are investigated. The annular plate is covered an electrorheological fluid core layer and a constraining layer to improve the stability of the system. The discrete layer annular finite element and the harmonic balance method are adopted to calculate the boundary of instability regions for the sandwich annular plate system. Besides, the rheological property of an electrorheological material, such as viscosity, plasticity, and elasticity can be changed when applying an electric field. When the electric field is applied on the sandwich structure, the damping of the sandwich system is more effective. Thus, variations of the instability regions for the sandwich annular plate with different applying electric fields, thickness of ER layer, and some designed parameters are presented and discussed in this study. The ER fluid core is found to have a significant effect on the location of the boundaries of the instability regions.
Vibration characteristic analysis of sandwich cylindrical shells with MR elastomer
Jia-Yi Yeh 국제구조공학회 2016 Smart Structures and Systems, An International Jou Vol.18 No.2
The vibration characteristic analysis of sandwich cylindrical shells subjected with magnetorheological (MR) elastomer and constraining layer are considered in this study. And, the discrete finite element method is adopted to calculate the vibration and damping characteristics of the sandwich cylindrical shell system. The effects of thickness of the MR elastomer, constraining layer, applied magnetic fields on the vibration characteristics of the sandwich shell system are also studied in this paper. Additionally, the rheological properties of the MR elastomer can be changed by applying various magnetic fields and the properties of the MR elastomer are described by complex quantities. The natural frequencies and modal loss factor of the sandwich cylindrical shells are calculated for many designed parameters. The core layer of MR elastomer is found to have significant effects on the damping behavior of the sandwich cylindrical shells.