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Jia, Gang,Jiang, RongChuan,Wang, KangNing Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.8
This study was conducted according to the single-factor design principle to investigate in vitro the effects of different glucagon-like peptide-2 (GLP-2) concentrations (0, $1{\times}10^{-11}$, $1{\times}10^{-10}$, $1{\times}10^{-9}$, $1{\times}10^{-8}$ and $1{\times}10^{-7}$ mol/L) on the morphology, proliferation and enzyme activity of intestinal enterocyte cells of 28-d-old weaned piglets. These cells were primary cultured in 4 pieces of 24-well cell culture plate. After having been grown for 48 h in culture media with hGLP-2, the ileal enterocyte cells of 28-d-old weaned piglets exhibited the typical characteristics of simple columnar epithelium. Compared with the control groups, the quantities of treated cells significantly increased (p<0.05) and their corresponding absorption values in 540 nm (MTT OD) also significantly increased (p<0.01). Likewise, lactic acid concentration, total protein content and protein retention significantly increased (p<0.05). $Na^{+}$, $K^{+}$-ATP enzyme activity was more active (p<0.05), although the activity of alkaline phosphatase, lactic acid dehydrogenase and creatine phosphokinase in culture media significantly decreased (p<0.01). To summarize, the results indicated that GLP-2 in vitro is capable of promoting the proliferation of intestinal enterocyte cells of 28-d weaned piglets, restraining their apoptosis and maintaining the integrity of their morphology.
Gang Jia,RongChuan Jiang,KangNing Wang 아세아·태평양축산학회 2009 Animal Bioscience Vol.22 No.8
This study was conducted according to the single-factor design principle to investigate in vitro the effects of different glucagon-like peptide-2 (GLP-2) concentrations (0, 1×10-11, 1×10-10, 1×10-9, 1×10-8 and 1×10-7 mol/L) on the morphology, proliferation and enzyme activity of intestinal enterocyte cells of 28-d-old weaned piglets. These cells were primary cultured in 4 pieces of 24-well cell culture plate. After having been grown for 48 h in culture media with hGLP-2, the ileal enterocyte cells of 28-d-old weaned piglets exhibited the typical characteristics of simple columnar epithelium. Compared with the control groups, the quantities of treated cells significantly increased (p<0.05) and their corresponding absorption values in 540 nm (MTT OD) also significantly increased (p<0.01). Likewise, lactic acid concentration, total protein content and protein retention significantly increased (p<0.05). Na+, K+-ATP enzyme activity was more active (p<0.05), although the activity of alkaline phosphatase, lactic acid dehydrogenase and creatine phosphokinase in culture media significantly decreased (p<0.01). To summarize, the results indicated that GLP-2 in vitro is capable of promoting the proliferation of intestinal enterocyte cells of 28-d weaned piglets, restraining their apoptosis and maintaining the integrity of their morphology.
PACKET RADIO NETWORK TECHNOLOGY AND ITS DESIGN
Gang, Wang,Lou, Jia Shi 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1
Packet Radio Networks have been developed for ten years and more. Today, many users and researchers are interested in this technology. This paper introduces mainly Packet Radio Network technology and some issues about its design. There are many design choices that must be made in the development of a packet radio network. The decisions are dependent on the environment that the network must work in, the requirements for performance and other functionalities, and the cost and other limitations. In addition, as new hardware and software technologies become available, the parameters governing the decisions change and often result in different selections. The intent of this paper is to provide an overview of the design decisions that must be made so as to provide a context for the decisions made in a number of existing and developing packet radio networks.
An Archaeal NADH Oxidase Causes Damage to Both Proteins and Nucleic Acids under Oxidative Stress
Jia, Baolei,Lee, Sang-Min,Pham, Bang P.,Cho, Yoon-Seung,Yang, Jae-Kyung,Byeon, Hee-Seop,Kim, Jong-Cheol,Cheong, Gang-Won Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.29 No.4
NADH oxidases (NOXs) catalyze the two-electron reduction of oxygen to $H_2O_2$ or four-electron reduction of oxygen to $H_2O$. In this report, we show that an NADH oxidase from Thermococcus profundus (NOXtp) displays two forms: a native dimeric protein under physiological conditions and an oxidized hexameric form under oxidative stress. Native NOXtp displays high NADH oxidase activity, and oxidized NOXtp can accelerate the aggregation of partially unfolded proteins. The aggregates formed by NOXtp have characteristics similar to ${\beta}$-amyloid and Lewy bodies in neuro-degenerative diseases, including an increase of ${\beta}$-sheet content. Oxidized NOXtp can also bind nucleic acids and cause their degradation by oxidizing NADH to produce $H_2O_2$. Furthermore, Escherichia coli cells expressing NOXtp are less viable than cells not expressing NOXtp after treatment with $H_2O_2$. As NOXtp shares similar features with eukaryotic cell death isozymes and life may have originated from hyperthermophiles, we suggest that NOXtp may be an ancestor of cell death proteins.
Experimental Study on the Shear Behaviors of PolypropyleneFiber-Reinforced Sand
Gang Li,Jinli Zhang,Jia Liu 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.12
With excellent water permeability and high shear strength S, fiber-reinforced sand (FRS) is an ideal material for subgrades and has promising application prospects. In this study, the FRS were formed by adding the randomly distributed fibers to the sand specimens. Based on the triaxial consolidated drained tests, the effects of factors such as fiber content ζ, fiber length lf, relative density Dr and confining pressure σ3 on the S of FRS were systematically investigated. The test results showed that the S of the FRS increased as ζ, lf, Dr and σ3 increased. Under axial loads, the stress-strain curve of the FRS exhibited a hardening trend. Under specific Dr and σ3, the S increment of the FRS had a strong linear relationship with ζ and lf. The Mohr's stress circle results showed that the FRS possessed not only frictional strength but also high cohesive strength. Based on the triaxial consolidated drained test results, the S of the FRS was calculated using the Zornberg model and Michalowski model. The calculation results showed that the S predicated by the Zornberg model differed significantly from the measured values. However, the S predicted by the Michalowski model were in good agreement with the measured values.
Analysis of Offensive Strategy in Soccer Video
Jia Wang,Haitao Yang,Miao Wang,Gang Liu 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.11
A novel Real-world trajectory based tactic analysis method is proposed, which can automatically analyze the offensive tactics of soccer game from the perspective of professionals. First, a real-world trajectory extraction method is proposed, and then an offensive pattern recognition method is described based on the definition of the ball states. Our experiments on user study by soccer professionals demonstrate that the defined offensive patterns can be used to analyze soccer tactics effectively in terms of conciseness, clarity and usability.
Rock-breaking analysis model of new drill bit with tornado-like bottomhole model
Jia-lin Tian,Chang-fu Yuan,Lin Yang,Chuan-hong Fu,Gang Liu,Zhi Yang,Chun-ming Wu 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.4
For improving the rock-breaking efficiency of oil and gas drill bits, a new drill bit is presented with tornado-like bottomhole model(named swirling cutting bit). The new drill bit cutter breaks rock with shocking and cutting effect during the drilling process, and theelements on largest ring cross the borehole center with high speed. It can effectively improve the center rock-breaking efficiency. Meanwhile,for all cutters on different rings breaking rock at the same time, it can optimize the rock-breaking volume of each element andimprove the bit service life. To analyze the interaction features between cutters and rock, the position equations are established by thecylindrical coordinates and complex movement principles, and then the velocity and acceleration equations can be obtained. Based on thenumerical example results, this paper analyzes the bottomhole model, the contact section, and the distribution features of velocities andaccelerations on different rings. By analyzing the acceleration results, we can study the failure mechanism of cutters. At the same time,lab experiments test the bottomhole model and rock-breaking features, and it verifies the accuracy of calculation method and equations. Moreover, the analysis method and models are also applicable to other types of bits or composite bits, and the inputting parameters needcorresponding adjustment for different type of bits.
Optimal Objects of Cooperation Selection for Human Activity in Opportunistic Networks
Jia Wu,Zhi-gang Chen,Xi Yi 한국산학기술학회 2014 SmartCR Vol.4 No.2
Randomness and disconnections during transmission in opportunistic networking have some characteristics similar to human activity. Many traditional methods in opportunistic networks, however, have never achieved effective results when applied to human activity. Consequently, this paper establishes and analyzes a structure tree and then, based on count dependability and usability in personal relationships, an optimized cooperation path is selected. Furthermore, the paper presents a new algorithm?the Optimal Cooperation Path Algorithm ?for opportunistic networks. This algorithm solves the problem of how to choose an appropriate path for human activity in opportunistic networks. When tested during simulations, this algorithm achieved good results.
Jia Yang,Jiajia Hao,Chen Tang,Yaxin Guo,Mingxin Guo,Zhipeng Li,Shuzheng Liu,Hui Yu,Gang Qin,Qiang Chen 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.116 No.-
Although many hydrogels have been applied to wearable sensors, it is still challenging to simultaneouslyrealize hydrogels with optical transparency, superior mechanical properties, excellent sensing performance,and anti-freezing by using inexpensive raw materials and an easy preparation process. Herein,using ethylene glycol/H2O (EG/ H2O) as a solvent, poly(vinyl alcohol)/EG organohydrogel (PVA/EGOHG) was prepared by a simple heating and frozen-thawing method. Owing to the multifunctionalityof EG (i.e., physical cross-linker, anti-freezer and co-solvent), PVA/EG OHG demonstrated excellent integratedproperties, including high strength, high toughness, and anti-freezing performances. Besides, PVA/EG OHG also showed low friction, self-cleaning, and frost resistance properties. After the introduction ofLiCl, ionically conductive PVA/EG @LiCl organohydrogel was served as a self-cleaning strain sensor, whichcould be long-term stable to detect the motions of human under room and low temperatures. The studyprovides to further understanding of the organohydrogel, which will help us design next-generationhigh-performance organohydrogels.