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Effect of Light with Different Wavelengths on Nostoc flagelliforme Cells in Liquid Culture
( Yu Jie Dai ),( Jing Li ),( Shu Mei Wei ),( Nan Chen ),( Yu Peng Xiao ),( Zhi Lei Tan ),( Shi Ru Jia ),( Nan Nan Yuan ),( Ning Tan ),( Yi Jie Song ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.4
The effects of lights with different wavelengths on the growth and the yield of extracellular polysaccharides of Nostoc flagelliforme cells were investigated in a liquid cultivation. N. flagelliforme cells were cultured for 16 days in 500 ml conical flasks containing BG11 culture medium under 27 μmol·m-2·s-1 of light intensity and 25oC on a rotary shaker (140 rpm). The chlorophyll a, phycocyanin, allophycocyanin, and phycoerythrin contents in N. flagelliforme cells under the lights of different wavelengths were also measured. It was found that the cell biomass and the yield of polysaccharide changed with different wavelengths of light. The biomass and the yield of extracellular polysaccharides under the red or violet light were higher than those under other light colors. Chlorophyll a, phycocyanin, and allophycocyanin are the main pigments in N. flagelliforme cells. The results showed that N. flagelliforme, like other cyanobacteria, has the ability of adjusting the contents and relative ratio of its pigments with the light quality. As a conclusion, N. flagelliforme cells favor red and violet lights and perform the complementary chromatic adaptation ability to acclimate to the changes of the light quality in the environment.
Jia-Jia Lin,Young-Hyun Han,Jung-Woo Kwon,Yong-Nan Xu,Yi-Bo Luo,Yu-Jin Jo,Chang-Eun Park,Jung-Kyu Baang,Suk Namgoong,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2014 Reproductive & Developmental Biology(Supplement) Vol.38 No.2s
In meiosis, Emi2 plays important role as CSF (Cytostatic Factor) to make the oocyte arrested in mII stage by the inhibition of APC/C (anaphase promoting complex/cyclosome). Once the oocyte fertilized, Emi2 was destabilized and degraded. For the degradation of Emi2, calcium signaling activate calmodulin-dependent protein kinase (CaMK) and phosphorylate emi2. Phosphorylated emi2 is recognized by polo-box domain of polo-like kinase 1 (Plk1) and further degradated by ubiquitin-dependent proteolysis. But recognition of Plk1 and emi2 is unknown. In this works, we determined the high-resolution crystal structure of polo-box domain of Plk1 and phosphorylated emi2 peptide at 1.90Å. Determined structure revealed that several unique features, including binding of Phe169 in the tyrosin-rich hydrophobic pocket. This is the first report of crystallization that Plk1-emi2 complex. Based on the complex structure, we designed the peptide analogs which pontentially inhibits recognition of Emi2 by Plk1 and assessed its biological activity in oocyte maturation and pathernogenetic activation. Injection of AB103-8, the inhibitor of Plk1 Polo-box domain, in mouse oocytes, induced the maturation arrest in GV stage and the delay in mII parthenogenetic activation. Further investigations of the mechanism that Plk1 involved into the Emi2 mII arrest are underway.
Yu Jia 아시아사회과학학회 2022 Jornal of Asia Social Science Vol.8 No.2
The initial intention of education is to cultivate people with virtue, and career planning in colleges and universities is the most important basic course for college students. Under the background of ideological and political education, it is an important task for colleges and universities to build a career planning curriculum system. The purpose of this paper is to construct the curriculum system of career planning from the aspects of curriculum objectives, curriculum contents, teaching staff construction and curriculum evaluation under the guidance of ideological and political spirit.
Yu Li,Dong-Shuo Yin,Jia-Hao Wang,Jia-Wu Li 한국강구조학회 2022 International Journal of Steel Structures Vol.22 No.5
Currently, the problem of human-induced vibration of suspension footbridges is a research hotspot in bridge engineering. Moreover, on the suspension footbridge deck, the walking parameters of pedestrians, the simulation of random pedestrian fl ow, and the calculation method of human-induced vibration are the keys to solving the above problem. Therefore, in this paper, the following three studies have been carried out: (1) Firstly, the Jiufengshan suspension footbridge was taken as the observation site, and the statistical values of the step frequency ( f ), step length ( l ), and walking velocity ( v ) of pedestrians on the suspension footbridge deck were investigated and obtained. (2) Secondly, by combining the “social force model” with the obtained f , l , and v , a Matlab program was compiled to simulate the random pedestrian fl ow on the suspension footbridge deck. (3) Finally, by combining the above program with the vertical vibration equations of the human–footbridge coupling system, a Matlab program was compiled to calculate the vertical human-induced vibration of the suspension footbridge deck, which then was used to study the infl uence of the random pedestrian fl ow and human–footbridge coupling eff ect on the vertical human-induced vibration of the suspension footbridge deck. So, the above studies can provide a meaningful reference for the design of suspension footbridges.
Yu Hong Jia,Jae Hun Ryu,Cho Hui Kim,Woo Kyung Lee,Thi Van Trinh Tran,Hyo Lee Lee,Rui Hong Zhang,안대희 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.2
Microbial electrolysis cell is a device which can produce hydrogen gas from biomass through microbial catalyzed process and thus reduce the organic matter. For the real application in wastewater treatment,the scale-up of microbial electrolysis cell is an important issue but few tests were conducted with relatively large size. In this study, a 3.7 L microbial electrolysis cell (liquid volume 3.2 L) equipped with a membrane electrode assembly cathode was designed and tested. The internal resistance was examined,hydrogen generation and organic removal performance was investigated under different conditions. A maximum overall hydrogen efficiency of 41% was achieved at an applied voltage of 1.2 V with acetate as substrate, corresponding to a volumetric hydrogen production rate of approximately 0.12 m3 H2/m3reactor liquid volume/day. The results obtained in this study could help to further develop pilot-MEC for practical applications.
Jia, Yu Hong,Ryu, Jae Hun,Kim, Cho Hui,Lee, Woo Kyung,Tran, Thi Van Trinh,Lee, Hyo Lee,Zhang, Rui Hong,Ahn, Dae Hee Elsevier 2012 Journal of industrial and engineering chemistry Vol.18 No.2
<P><B>Abstract</B></P><P>Microbial electrolysis cell is a device which can produce hydrogen gas from biomass through microbial catalyzed process and thus reduce the organic matter. For the real application in wastewater treatment, the scale-up of microbial electrolysis cell is an important issue but few tests were conducted with relatively large size. In this study, a 3.7L microbial electrolysis cell (liquid volume 3.2L) equipped with a membrane electrode assembly cathode was designed and tested. The internal resistance was examined, hydrogen generation and organic removal performance was investigated under different conditions. A maximum overall hydrogen efficiency of 41% was achieved at an applied voltage of 1.2V with acetate as substrate, corresponding to a volumetric hydrogen production rate of approximately 0.12m<SUP>3</SUP> H<SUB>2</SUB>/m<SUP>3</SUP> reactor liquid volume/day. The results obtained in this study could help to further develop pilot-MEC for practical applications.</P>
Yu, Weiyang,Zhu, Zhili,Niu, Chun-Yao,Li, Chong,Cho, Jun-Hyung,Jia, Yu Springer US 2016 NANOSCALE RESEARCH LETTERS Vol.11 No.1
<P>We present first-principles density-functional calculations for the structural, electronic, and magnetic properties of substitutional 3<I>d</I> transition metal (TM) impurities in two-dimensional black and blue phosphorenes. We find that the magnetic properties of such substitutional impurities can be understood in terms of a simple model based on the Hund’s rule. The TM-doped black phosphorenes with Ti, V, Cr, Mn, Fe, and Ni impurities show dilute magnetic semiconductor (DMS) properties while those with Sc and Co impurities show nonmagnetic properties. On the other hand, the TM-doped blue phosphorenes with V, Cr, Mn, and Fe impurities show DMS properties, with Ni impurity showing half-metal properties, whereas Sc- and Co-doped systems show nonmagnetic properties. We identify two different regimes depending on the occupation of the hybridized electronic states of TM and phosphorous atoms: (i) bonding states are completely empty or filled for Sc- and Co-doped black and blue phosphorenes, leading to nonmagnetic; (ii) non-bonding <I>d</I> states are partially occupied for Ti-, V-, Cr-, Mn-, Fe- and Ni-doped black and blue phosphorenes, giving rise to large and localized spin moments. These results provide a new route for the potential applications of dilute magnetic semiconductor and half-metal in spintronic devices by employing black and blue phosphorenes.</P><P><B>PACS numbers:</B> 73.22.-f, 75.50.Pp, 75.75. + a</P>