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Kang Zhi‐Wei,Liu Fang‐Hua,Xu Yong‐Yu,Cheng Jia‐Hui,Lin Xiao‐Li,Jing Xiang‐Feng,Tian Hong‐Gang,Liu Tong‐Xian 한국곤충학회 2021 Entomological Research Vol.51 No.1
Odorant‐degrading enzymes (ODEs) have been found in insect antennae and play a critical role in signal chemical degradation once the message is conveyed. Significant progress has been made in characterizing ODEs in a variety of pests but very little is known in their natural enemies. We have carried out an antennae‐ and sex‐specific transcriptome of Aphidius gifuensis, a natural enemy of aphid, to identify the candidate ODEs. Based on the antennae‐ and sex‐specific transcriptome, a total of 100 putative ODEs were identified including one aldehyde oxidase (AOX), four alcohol dehydrogenases (ADs), eight UDP‐glucuronosyltransferases (UGTs), 45 cytochrome P450 (P450s), nine glutathione S‐transferases (GSTs) and 40 carboxylesterases (CCEs or CXEs). Additionally, we used RT‐qPCR to determine the expression profiles of these genes in tissues of both sexes. Based on the phylogenic analysis and tissue‐expression patterns, AgifEstE4, AgifCXE3, AgifCCE4, AgifCCE7, and AgifCCE18 were suggested as key ODEs in A. gifuensis. In addition, the female or male specifically enriched genes, such as AgifCCE17, AgifEstB1, AgifCYP18a1, AgifUGT2C2, were also considered to involve in the chemosensory processing in A. gifuensis. This study not only identified the candidate ODEs in A. gifuensis but also provided source for further exploration of the molecular mechanisms of chemical signal transductions in A. gifuensis, as well as other hymenopteran species.
Jia-Wei Lv,Xiao-Dan Huang,Yu-Pei Chen,Guan-Qun Zhou,Ling-Long Tang,Yan-Ping Mao,Wen-Fei Li,Ai-Hua Lin,Jun Ma,Ying Sun 대한암학회 2018 Cancer Research and Treatment Vol.50 No.2
Purpose Conditional survival (CS) provides important information on survival for a period of time after diagnosis. Currently, information on CS patterns of patients with nasopharyngeal carcinoma (NPC) is lacking. We aimed to analyze survival rate over time and estimate CS for NPC patients using a national population-based registry. Materials and Methods Patients diagnosed with NPC between 1973 and 2007 with at least 5-year follow-up were identified from the Surveillance Epidemiology End Results registry. Traditional survival rates and crude CS estimates were calculated using Kaplan-Meier analysis. Risk-adjusted survival curves were plotted from the proportional hazards model using the correct group prognosis method. Results For 7,713 patients analyzed, adjusted baseline 5-year overall survival improved significantly from 36.0% in patients diagnosed in 1973-1979, 41.7% in 1980-1989, 46.6% in 1990- 1999, to 54.7% in 2000-2007 (p < 0.01). CS analysis demonstrated that for every additional year survived, adjusted probability of surviving the next 5 years increased from 66.7% (localized), 54.0% (regional), and 35.3% (distant) at the time of diagnosis, to 83.7% (localized), 75.0% (regional), and 62.2% (distant) for patients who had survived 5 years. Adjusted 5-year CS differed among age, sex, tumor histology, ethnicity, and stage subgroups initially, but converged with time. Conclusion Treatment outcomes of NPC patients have greatly improved over the decades. Increases in CS become more prominent in patients with distant disease than in those with localized or regional disease as patients survive longer. CS provides more dynamic prognostic information for patients who have survived a period of time after diagnosis.
Jia, Wenbin,Zhu, Xiaoping,Zhang, Wei,Cheng, Jianbo,Guo, Cuihua,Jia, Zhihai Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.12
This experiment was designed to evaluate the effects of source of supplemental zinc (Zn) on performance, nutrient digestibility and plasma mineral profile in Cashmere goats during the cashmere fiber growing period. Twenty-seven Liao Ning Cashmere wether goats (9-10 month of age; initial BW = 19.31${\pm}$0.32 kg) were fed a basal diet (containing 22.3 mg Zn/kg DM) with no supplemental Zn (control) or 20 mg of supplemental Zn/kg of DM from Zn sulfate ($ZnSO_{4}$) or Zn methionine (ZnMet) for 60 days including a 10-day metabolism trial. Average daily gain (ADG) (p<0.05) and gain:feed (G/F) (p<0.05) were increased by Zn supplementation, but no differences were noted between Zn sources (p>0.05). The length and diameter of cashmere fiber did not differ among treatments (p>0.05). Zn supplementation had no influence on digestibility of DM, CP, EE and NDF (p>0.05). However, ADF digestibility in the group supplemented with ZnMet was significantly higher than in other treatments (p<0.05). Plasma Zn was increased (p<0.05) and Cu tended to be decreased (p = 0.057) by Zn supplementation, but no differences were found between Zn sources (p>0.05). Plasma alkaline phosphatase activity (AKP) was improved by Zn supplementation (p<0.05) and was higher in the $ZnSO_{4}$ than the ZnMet group (p<0.05). Zn retention was increased (p<0.05) and apparent absorption rate was decreased (p<0.05) by Zn supplementation. The results indicate that supplementation of 20 mg Zn/kg DM either as $ZnSO_{4}$ or ZnMet in the basal diet containing 22.3 mg Zn/kg DM can improve growth performance in Cashmere goats, and effectiveness of the two sources is similar on performance measurements.
Serum Proteomics Analysis of Feline Mammary Carcinoma Based on Label-free and PRM Techniques
Jia-San Zheng,Ren-Yue Wei,Zheng Wang,Ting-Ting Zhu,Hong-Ri Ruan,Xue Wei,Kai-Wen Hou,Rui Wu 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.3
Background: Feline mammary carcinoma is the third most common cancer that affects female cats. Objectives: The purpose of this study was to screen differential serum proteins in feline and clarify the relationship between them and the occurrence of feline mammary carcinoma. Methods: Chinese pastoral cats were used as experimental animals. Six serum samples from cats with mammary carcinoma (group T) and six serum samples from healthy cats (group C) were selected. Differential protein analysis was performed using a Label-free technique, while parallel reaction monitoring (PRM) was performed to verify the screened differential proteins. Results: A total of 82 differential proteins were detected between group T and group C, of which 55 proteins were down regulated and 27 proteins were up regulated. Apolipoprotein A-I, Apolipoprotein A-II (ApoA-II), Apolipoprotein B (ApoB), Apolipoprotein C-III (ApoC-III), coagulation factor V, coagulation factor X, C1q, albumen (ALB) were all associated with the occurrence of feline mammary carcinoma. Differential proteins were involved in a total of 40 signaling pathways, among which the metabolic pathways associated with feline mammary carcinoma were the complement and coagulation cascade and cholesterol metabolism. According to the Label-free results, ApoB, ApoC-III, ApoA-II, FN1, an uncharacterized protein, and ALB were selected for PRM target verification. The results were consistent with the trend of the label-free. Conclusions: This experimen is the first to confirm ApoA-II and ApoB maybe new feline mammary carcinoma biomarkers and to analyze their mechanisms in the development of such carcinoma in feline.
Wei Jia,Qingyi Hua,Minjun Zhang,Rui Chen,Xiang Ji,Bo Wang 한국정보처리학회 2019 Journal of information processing systems Vol.15 No.4
Mobile user interface pattern (MUIP) is a kind of structured representation of interaction design knowledge. Several studies have suggested that MUIPs are a proven solution for recurring mobile interface design problems. To facilitate MUIP selection, an effective clustering method is required to discover hidden knowledge of patterndata set. In this paper, we employ the semi-supervised kernel fuzzy c-means clustering (SSKFCM) method tocluster MUIP data. In order to improve the performance of clustering, clustering parameters are optimized byutilizing the global optimization capability of particle swarm optimization (PSO) algorithm. Since the PSOalgorithm is easily trapped in local optima, a novel PSO algorithm is presented in this paper. It combines animproved intuitionistic fuzzy entropy measure and a new population search strategy to enhance the populationsearch capability and accelerate the convergence speed. Experimental results show the effectiveness andsuperiority of the proposed clustering method.