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Heat Stress Causes Aberrant DNA Methylation of H19 and Igf-2r in Mouse Blastocysts
Jia-Qiao Zhu,Jing-He Liu,Xing-Wei Liang,Bao-Zeng Xu,Yi Hou,Xing-Xu Zhao,Qing-Yuan Sun 한국분자세포생물학회 2008 Molecules and cells Vol.25 No.2
To gain a better understanding of the methylation imprinting changes associated with heat stress in early development, we used bisulfite sequencing and bisulfite restriction analysis to examine the DNA methylation status of imprinted genes in early embryos (blastocysts). The paternal imprinted genes, H19 and Igf-2r, had lower methylation levels in heat-stressed embryos than in control embryos, whereas the maternal imprinted genes, Peg3 and Peg1, had similar methylation pattern in heat-stressed embryos and in control embryos. Our results indicate that heat stress may induce aberrant methylation imprinting, which results in developmental failure of mouse embryos, and that the effects of heat shock on methylation imprinting may be gene-specific.
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An Efficient PEG/CaCl2-Mediated Transformation Approach for the Medicinal Fungus Wolfiporia cocos
( Qiao Sun ),( Wei Wei ),( Juan Zhao ),( Jia Song ),( Fang Peng ),( Shao Peng Zhang ),( Yong Lian Zheng ),( Ping Chen ),( Wen Jun Zhu ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.9
Sclerotia of Wolfiporia cocos are of medicinal and culinary value. The genes and molecular mechanisms involved in W. cocos sclerotial formation are poorly investigated because of the lack of a suitable and reproducible transformation system for W. cocos. In this study, a PEG/ CaCl2-mediated genetic transformation system for W. cocos was developed. The promoter Pgpd from Ganoderma lucidum effectively drove expression of the hygromycin B phosphotransferase gene in W. cocos, and approximately 30 transformants were obtained per 10 μg DNA when the protoplast suspension density was 106 protoplasts/ml. However, no transformants were obtained under the regulation of the PtrpC promoter from Aspergillus nidulans.
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Qiao, Ying,Lu, Zong-Xiang,Lu, Ji,Ruan, Jia-Yang,Wu, Lin-lin The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.6
The integration of the large-scale wind power brings great challenge to the stability of the power grid. This paper investigates and studies the fault on May 14, 2012 of the large-scale cascading trip-off of wind turbines in North China. According to the characteristics of the voltage variation, the fault process is divided into three stages: the pre-event stage, the critical stage before cascading, and the cascading stage. The scenes in the fault are reproduced, using the full-size actual power system model. Simulation models of double-fed induction generators (DFIGs) and SVCs including protection settings and controller strategies are carefully chosen to find out the reason of voltage instability in each stage. Some voltage dynamic that have never been observed before in the faults of the same kind are analyzed in detail, and an equivalent voltage sensitive dynamic model of DFIG is proposed for the fast computation. The conclusions about the voltage dynamics are validated by the actual PMU observation evidence.
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Improve Efficiency and Reliability in Single-Hop WSNs with Transmit-Only Nodes
Jia Zhao,Chunming Qiao,Sudhaakar, R. S.,Seokhoon Yoon IEEE 2013 IEEE transactions on parallel and distributed syst Vol.24 No.3
<P>Wireless Sensor Networks (WSNs) will play a significant role at the “edge” of the future “Internet of Things.” In particular, WSNs with transmit-only nodes are attracting more attention due to their advantages in supporting applications requiring dense and long-lasting deployment at a very low cost and energy consumption. However, the lack of receivers in transmit-only nodes renders most existing MAC protocols invalid. Based on our previous study on WSNs with pure transmit-only nodes, this work proposes a simple, yet cost effective and powerful single-hop hybrid WSN cluster architecture that contains not only transmit-only nodes but also standard nodes (with transceivers). Along with the hybrid architecture, this work also proposes a new MAC layer protocol framework called Robust Asynchronous Resource Estimation (RARE) that efficiently and reliably manages the densely deployed single-hop hybrid cluster in a self-organized fashion. Through analysis and extensive simulations, the proposed framework is shown to meet or exceed the needs of most applications in terms of the data delivery probability, QoS differentiation, system capacity, energy consumption, and reliability. To the best of our knowledge, this work is the first that brings reliable scheduling to WSNs containing both nonsynchronized transmit-only nodes and standard nodes.</P>
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Ying Qiao,Zong-Xiang Lu,Ji Lu,Jia-Yang Ruan,Lin-lin Wu 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.6
The integration of the large-scale wind power brings great challenge to the stability of the power grid. This paper investigates and studies the fault on May 14, 2012 of the large-scale cascading trip-off of wind turbines in North China. According to the characteristics of the voltage variation, the fault process is divided into three stages: the pre-event stage, the critical stage before cascading, and the cascading stage. The scenes in the fault are reproduced, using the full-size actual power system model. Simulation models of double-fed induction generators (DFIGs) and SVCs including protection settings and controller strategies are carefully chosen to find out the reason of voltage instability in each stage. Some voltage dynamic that have never been observed before in the faults of the same kind are analyzed in detail, and an equivalent voltage sensitive dynamic model of DFIG is proposed for the fast computation. The conclusions about the voltage dynamics are validated by the actual PMU observation evidence.
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Experimental and numerical study on shear studs connecting steel girder and precast concrete deck
Shengfang Qiao,Huqing Liang,Jia-Bao Yan,Wanying Wang 국제구조공학회 2019 Structural Engineering and Mechanics, An Int'l Jou Vol.71 No.4
Shear studs are often used to connect steel girders and concrete deck to form a composite bridge system. The application of precast concrete deck to steel-concrete composite bridges can improve the strength of decks and reduce the shrinkage and creep effect on the long-term behavior of structures. How to ensure the connection between steel girders and concrete deck directly influences the composite behavior between steel girder and precast concrete deck as well as the behavior of the structure system. Compared with traditional multi-I girder systems, a twin-I girder composite bridge system is more simplified but may lead to additional requirements on the shear studs connecting steel girders and decks due to the larger girder spacing. Up to date, only very limited quantity of researches has been conducted regarding the behavior of shear studs on twin-I girder bridge systems. One convenient way for steel composite bridge system is to cast concrete deck in place with shear studs uniformly-distributed along the span direction. For steel composite bridge system using precast concrete deck, voids are included in the precast concrete deck segments, and they are casted with cast-in-place concrete after the concrete segments are erected. In this paper, several sets of push-out tests are conducted, which are used to investigate the heavier of shear studs within the voids in the precast concrete deck. The test data are analyzed and compared with those from finite element models. A simplified shear stud model is proposed using a beam element instead of solid elements. It is used in the finite element model analyses of the twin-I girder composite bridge system to relieve the computational efforts of the shear studs. Additionally, a parametric study is developed to find the effects of void size, void spacing, and shear stud diameter and spacing. Finally, the recommendations are given for the design of precast deck using void for twin I-girder bridge systems.
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Wang, Jia-Yi,Zhou, Yu-Qiao,Li, Xiao-Xu,Jin, Xin,Wang, Li-Li,Lei, Lei,Zhou, Yu,Lu, Jiang,Zeng, Xin,Dan, Hong-Xia,Liao, Ga,Chen, Qian-Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12
Interleukin-4 receptor (IL-4R) gene single nucleotide polymorphisms (SNPs) are implicated in cancer development. However, results from the published reports have remained inconclusive. The objective of this study was to conduct a meta-analysis investigating the association between polymorphisms in IL-4R gene and cancer risk. Pubmed, EMBASE and China National Knowledge Infrastructure (CNKI) were searched for case-control studies published up to October 30, 2012 that investigated IL-4R polymorphisms and cancer risk. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to evaluate the strength of any associations. Three IL-4R polymorphisms (Q576R, rs1801275; I75V, rs1805010; S503P, rs1805015) in 21 case-control studies were analyzed. Our meta-analysis indicated that these three polymorphisms are not associated with cancer risk when all studies were pooled together. In the subgroup analysis by tumor site, the results showed that Q576R G allele carriers were associated with a significantly decreased cervical cancer risk (recessive model: OR = 0.77, 95%CI = 0.60-0.98; homozygote comparison: OR = 0.76, 95%CI = 0.58-0.98). I75V G allele carriers were associated with a decreased risk of renal cancer (dominant model = 0.71, 95%CI = 0.57-0.89, heterozygote comparison: OR = 0.69, 95%CI = 0.55-0.87). When stratified by ethnicity, Q576R G allele carriers were associated with a decreased cancer risk in Caucasians (dominant model: OR = 0.90, 95%CI = 0.83-0.98; heterozygote comparison: OR = 0.89, 95%CI = 0.82-0.98). I75V G allele carriers were associated with a decreased cancer risk in Asians (heterozygote comparison: OR = 0.76, 95%CI = 0.62-0.94). S503P C allele carriers were also associated with a decreased cancer risk in Asians (CC VS TT: OR = 0.29, 95%CI = 0.08-0.99). Our results suggest that Q576R, I75V and S503P may be associated with a decreased cancer risk for certain types of cancers and in some specific ethnic groups. Future case-control studies with large sample size are needed to evaluate these associations in detail.
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Lin, Jia,Chen, Hong,Gao, Yang,Cai, Yao,Jin, Jianbo,Etman, Ahmed S.,Kang, Joohoon,Lei, Teng,Lin, Zhenni,Folgueras, Maria C.,Quan, Li Na,Kong, Qiao,Sherburne, Matthew,Asta, Mark,Sun, Junliang,Toney, Mic National Academy of Sciences 2019 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.116 No.47
<P><B>Significance</B></P><P>Metal halide perovskites attract great interest for a wide range of applications due to their remarkable optoelectronic properties. The development of environmentally friendly halide perovskite materials with various crystal structures and compositions offers unprecedented opportunities to achieve desired properties and applications. In this work, we demonstrated an In-based, charge-ordered all-inorganic halide double perovskite with the composition of Cs<SUB>2</SUB>In(I)In(III)Cl<SUB>6</SUB> synthesized by solid-state reaction. High-pressure optical properties were studied, and a pressure-driven, fully reversible semiconductor–metal phase transition was discovered. This In-based charge-ordered structure may inspire new understanding of halide perovskite as well as provide a platform for future discovery of exotic electronic phenomena such as high-<I>T</I><SUB>C</SUB> superconductivity in halide perovskite compounds.</P><P>Phase transitions in halide perovskites triggered by external stimuli generate significantly different material properties, providing a great opportunity for broad applications. Here, we demonstrate an In-based, charge-ordered (In<SUP>+</SUP>/In<SUP>3+</SUP>) inorganic halide perovskite with the composition of Cs<SUB>2</SUB>In(I)In(III)Cl<SUB>6</SUB> in which a pressure-driven semiconductor-to-metal phase transition exists. The single crystals, synthesized via a solid-state reaction method, crystallize in a distorted perovskite structure with space group <I>I</I>4/<I>m</I> with <I>a</I> = 17.2604(12) Å, <I>c</I> = 11.0113(16) Å if both the strong reflections and superstructures are considered. The supercell was further confirmed by rotation electron diffraction measurement. The pressure-induced semiconductor-to-metal phase transition was demonstrated by high-pressure Raman and absorbance spectroscopies and was consistent with theoretical modeling. This type of charge-ordered inorganic halide perovskite with a pressure-induced semiconductor-to-metal phase transition may inspire a range of potential applications.</P>
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