FADD Phosphorylation Modulates Blood Glucose Levels by Decreasing the Expression of InsulinDegrading Enzyme

Lin, Yan,Liu, Jia,Chen, Jia,Yao, Chun,Yang, Yunwen,Wang, Jie,Zhuang, Hongqin,Hua, Zi-Chun Korean Society for Molecular and Cellular Biology 2020 Molecules and cells Vol.43 No.4

Our previous study revealed a novel role of Fas-associated death domain-containing protein (FADD) in islet development and insulin secretion. Insulin-degrading enzyme (IDE) is a zinc metalloprotease that selectively degrades biologically important substrates associated with type 2 diabetes (T2DM). The current study was designed to investigate the effect of FADD phosphorylation on IDE. We found that the mRNA and protein levels of IDE were significantly downregulated in FADD-D mouse livers compared with control mice. Quantitative real-time polymerase chain reaction analysis showed that FADD regulates the expression of IDE at the transcriptional level without affecting the stability of the mRNA in HepG2 cells. Following treatment with cycloheximide, the IDE protein degradation rate was found to be increased in both FADD-D primary hepatocytes and FADD-knockdown HepG2 cells. Additionally, IDE expression levels were reduced in insulin-stimulated primary hepatocytes from FADD-D mice compared to those from control mice. Moreover, FADD phosphorylation promotes nuclear translocation of FoxO1, thus inhibiting the transcriptional activity of the IDE promoter. Together, these findings imply a novel role of FADD in the reduction of protein stability and expression levels of IDE.

The multidirectional vibration and coupling dynamics of drill string and its influence on the wellbore trajectory

Lin Yang,Jia-lin Tian,Qingyou Liu,Liming Dai,Zhichao Hu,Jurui Li 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.7

For improving the accuracy of wellbore trajectory prediction in complex wellbore structures, especially in the kick-off section, a longitudinal, torsional and radial coupled drill string dynamics model is proposed. At the same time, the coupling dynamics model is solved based on the energy method, and the torque, weight on bit (WOB), the longitudinal displacement and velocity, the torsional angular displacement and angular velocity of the drill bit are obtained during the normal drilling and the stick-slip vibration phase. Combining the numerical example and experimental test, the differences between different models and actual drilling trajectories are compared and analyzed. The research results indicate that the torsional vibration and the longitudinal vibration have a great influence on each other in the coupling model, and the longitudinal vibration displacement increases, which can effectively reduce the torque variation range of the torsional vibration. The operation of the bit stagnation is weakened, maintaining a relatively stable rotational angular velocity of rotation. The calculation results of the wellbore trajectory are more consistent with the experimental results, and can accurately reflect the real situation of the drilling process. The theoretical model has an important reference for predicting the new well structure, especially the judgment of wellbore trajectory of complex wellbore structures such as extended reach wells, horizontal wells, deep wells and high deep wells of coalbed methane, and the prediction of downhole dynamics results.

Seismic performance assessment of steel reinforced concrete members accounting for double pivot stiffness degradation

Jia-Lin Juang,Hsieh-Lung Hsu 국제구조공학회 2008 Steel and Composite Structures, An International J Vol.8 No.6

This paper presents an effective hysteretic model for the prediction and evaluation of steel reinforced concrete member seismic performance. This model adopts the load-deformation relationship acquired from monotonic load tests and incorporates the double-pivot behavior of composite members subjected to cyclic loads. Deterioration in member stiffness was accounted in the analytical model. The composite member performance assessment control parameters were calibrated from the test results. Comparisons between the cyclic load test results and analytical model validated the proposed method’s effectiveness.

工伤保险与民事损害赔偿竞合问题之再检讨

Lin, Jia 경북대학교 법학연구원 2015 법학논고 Vol.0 No.50

The relationship of Work Injury Insurance and civil damage compensation is always under heated debate. In the perspective of civil law, it mainly concerns the amount of tort compensation. While in the perspective of social law, it is an issue of how to choose the mode of work injury relief. The litigators have not given a clear answer to this issue, which leads to different standards in practice and causes contradicts in judicial practice. Substantially, to choose the mode of work injury relief is a problem of value judging. This article believes that it better conforms to the goal of work injury relief and its value to adopt Compensation Mode. To achieve the goal of work injury relief, to better realize fairness and guarantee the human rights of labors, and to fit the developing trend of other countries all over the world, we shall adopt the mode of work injury insurance as priority, civil damage compensation as supplement and the right of recovery of social insurance institutions to the injurer as another supplement. Thus, Compensation Mode shall become the direction of China’s work injury relief. Therefore, we should also review on the Double Compensation Mode adopted in judicial practice currently. 工伤保险待遇与民事侵权损害赔偿的关系,历来是学界热议的问题。从民法的角度看此问题主要涉及侵权损害赔偿的数额,从社会法角度而言却事关工伤救济的模式选择问题。由于长期以来立法者对此问题一直未有明确表态,导致各地遵循不同的标准进行操作,由此引起司法实践中的混乱与矛盾。工伤救济模式的选择问题从本质上看是一个价值判断问题,笔者认为工伤救济采取“补偿模式”更为符合工伤救济所要达到的目的以及所承载的价值。以工伤保险优先、民事侵权赔偿作为补充、社会保险机构对过错加害人员具有追偿权的补充模式更有利于实现工伤救济的目的,更有利于实现公平和劳动者人权保障,也符合世界各国的发展趋势。因此,补充模式应该成为我国工伤救济模式的发展方向,当前对目前司法实践所采取的“双赔”模式进行检讨。

Photocatalytic study of Zinc Oxide with bismuth doping prepared by spray pyrolysis

Lin, Tzu-Yang,Hsu, Yu-Ting,Lan, Wen-How,Huang, Chien-Jung,Chen, Lung-Chien,Huang, Yu-Hsuan,Lin, Jia-Ching,Chang, Kuo-Jen,Lin, Wen-Jen,Huang, Kai-Feng Techno-Press 2015 Advances in nano research Vol.3 No.3

The unintentionally doped and bismuth (Bi) doped zinc oxide (ZnO) films were prepared by spray pyrolysis at $450^{\circ}C$ with zinc acetate and bismuth nitrate precursor. The n-type conduction with concentration $6.13{\times}10^{16}cm^{-3}$ can be observed for the unintentionally doped ZnO. With the increasing of bismuth nitrate concentration in precursor, the p-type conduction can be observed. The p-type concentration $4.44{\times}10^{17}cm^{-3}$ can be achieved for the film with the Bi/Zn atomic ratio 5% in the precursor. The photoluminescence spectroscopy with HeCd laser light source was studied for films with different Bi doping. The photocatalytic activity for the unintentionally doped and Bi-doped ZnO films was studied through the photodegradation of Congo red under UV light illumination. The effects of different Bi contents on photocatalytic activity are studied and discussed. Results show that appropriate Bi doping in ZnO can increase photocatalytic activity.

Experimental and analytical study on RC beam reinforced with SFCB of different fiber volume ratios under flexural loading

Jia-Xiang Lin,Yong-Jian Cai,Ze-Ming Yang,Shu-Hua Xiao,Zhan-Biao Chen 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.45 No.1

Steel fiber composite bar (SFCB) is a novel type of reinforcement, which has good ductility and durability performance. Due to the unique pseudo strain hardening tensile behavior of SFCB, different flexural behavior is expected of SFCB reinforced concrete (SFCB-RC) beams from traditional steel bar reinforced concrete (S-RC) beams and FRP bar reinforced concrete (F-RC) beams. To investigate the flexural behavior of SFCB-RC beam, four points bending tests were carried out and different flexural behaviors between S/F/SFCB-RC beams were discussed. An flexural analytical model of SFCB-RC beams is proposed and proved by the current and existing experimental results. Based on the proposed model, the influence of the fiber volume ratio R of the SFCB on the flexural behavior of SFCB-RC beams is discussed. The results show that the proposed model is effective for all S/F/SFCB-RC flexural members. Fiber volume ratio R is a key parameter affecting the flexural behavior of SFCB-RC. By controlling the fiber volume ratio of SFCB reinforcements, the flexural behavior of the SFCB-RC flexural members such as bearing capacity, bending stiffness, ductility and repairability of SFCB-RC structures can be designed.

An Enhanced Reversible Data Hiding Scheme based on Histogram Modification

Lin Jia(지아린),Gil-Je Lee(이길제),Kee-Young Yoo(유기영) 대한전자공학회 2010 대한전자공학회 학술대회 Vol.2010 No.6

In 2008, Lin et al. proposed the multilevel reversible data hiding scheme based on the histogram modification but many distortions are created due to the histogram shifting method which is used in the embedding phase. In this paper, the proposed scheme combines the peak point bin with the adjacent bin of the histogram to decrease the above distortions. The expermential results show that the proposed scheme achieves the better performance than Lin et al.’s scheme in both the stego image quality and the hiding capacity.

Diagnostic Value of Human Epididymis Protein 4 Compared with Mesothelin for Ovarian Cancer: a Systematic Review and Meta-analysis

Lin, Jia-Ying,Qin, Jin-Bao,Li, Xiao-Yan,Dong, Ping,Yin, Bing-De Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

Background and Purpose: Ovarian cancer is the leading cause of death among gynecologic cancers because of the lack of effective early detection methods. Accuracies of the human epididymis protein 4 (HE4) and mesothelin in detecting ovarian cancer have never been systematically assessed. The current systematic review aimed to tackle this issue. Methods: MEDLINE, EMBASE, and Cochrane databases were searched (September 1995-November 2011) for studies on the diagnostic performances of HE4 and mesothelin in differentiating ovarian cancer from other benign gynecologic diseases. QUADAS items were used to evaluate the qualities of the studies. Meta-DiSc software was used to handle data from the included studies and to examine heterogeneity. All included studies for diagnostic performance were combined with sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratios (DORs) with 95% confidence intervals (CIs), summary receiver operating characteristic (SROC) curves, and areas under the SROC curves (AUC). Results: A total of 18 studies and 3,865 patients were eligible for the final analysis. The pooled sensitivity estimates for HE4 (74.4%) were significantly higher than those for mesothelin (49.3%). The pooled specificity estimates for mesothelin (94.5%) were higher than those for HE4 (85.8%). The pooled DOR estimates for HE4 (26.22) were higher than those for mesothelin (24.01). The SROC curve for HE4 showed better diagnostic accuracy than that for mesothelin. The PLR and NLR of HE4 were 6.33 (95% CI: 3.58 to 11.18) and 0.27 (95% CI: 0.21 to 0.34), respectively. The PLR and NLR for mesothelin were 11.0 (95% CI: 6.21 to 19.59) and 0.51 (95% CI: 0.42 to 0.62), respectively. The combination of the two tumor markers or their combination with CA-125 increased sensitivity and specificity to different extents. Conclusion: The diagnostic accuracy of HE4 in differentiating ovarian cancer from other benign gynecologic diseases is better than that of soluble mesothelin-related protein. Combinations of two or more tumor markers show more sensitivity and specificity.

Structural Basis of Emi2 Recognition by Polo-Box Domain of Polo-like Kinase 1 and Effects of Structure-Derived Antagonist in Oocyte Maturation and Fertillization

Jia-Jia Lin,Young-Hyun Han,Jung-Woo Kwon,Yong-Nan Xu,Yi-Bo Luo,Yu-Jin Jo,Chang-Eun Park,Jung-Kyu Baang,Suk Namgoong,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2014 Reproductive & Developmental Biology(Supplement) Vol.38 No.2s

In meiosis, Emi2 plays important role as CSF (Cytostatic Factor) to make the oocyte arrested in mII stage by the inhibition of APC/C (anaphase promoting complex/cyclosome). Once the oocyte fertilized, Emi2 was destabilized and degraded. For the degradation of Emi2, calcium signaling activate calmodulin-dependent protein kinase (CaMK) and phosphorylate emi2. Phosphorylated emi2 is recognized by polo-box domain of polo-like kinase 1 (Plk1) and further degradated by ubiquitin-dependent proteolysis. But recognition of Plk1 and emi2 is unknown. In this works, we determined the high-resolution crystal structure of polo-box domain of Plk1 and phosphorylated emi2 peptide at 1.90Å. Determined structure revealed that several unique features, including binding of Phe169 in the tyrosin-rich hydrophobic pocket. This is the first report of crystallization that Plk1-emi2 complex. Based on the complex structure, we designed the peptide analogs which pontentially inhibits recognition of Emi2 by Plk1 and assessed its biological activity in oocyte maturation and pathernogenetic activation. Injection of AB103-8, the inhibitor of Plk1 Polo-box domain, in mouse oocytes, induced the maturation arrest in GV stage and the delay in mII parthenogenetic activation. Further investigations of the mechanism that Plk1 involved into the Emi2 mII arrest are underway.

Lentivirus-mediated microRNA-124 gene-modified bone marrow mesenchymal stem cell transplantation promotes the repair of spinal cord injury in rats

Jia-Lin Song,Wei Zheng,Wei Chen,Yun Qian,Yuan-Ming Ouyang,Cun-Yi Fan 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Our study aims to explore the effects of lentivirus-mediated microRNA-124 (miR-124) gene-modified bone marrow mesenchymal stem cell (BMSC) transplantation on the repair of spinal cord injury (SCI) in rats. BMSCs were isolated from the bone marrow of rats. The target gene miR-124 was identified using a luciferase-reporter gene assay. Seventy-two rats were selected for construction of the SCI model, and the rats were randomly divided into the blank group, sham group, SCI group, negative control (NC) group, overexpressed miR-124 group and si-PDXK group. The mRNA expression of miR-124 and the mRNA and protein expression of pyridoxal kinase (PDXK) were detected by quantitative real-time polymerase chain reaction and western blotting. The locomotor capacity of the rats was evaluated using the Basso, Beattie and Bresnahan (BBB) scale. Brdu, neuron-specific enolase (NSE), neurofilament (NF) and microtubule-associated protein 2 (MAP2) were detected using immunohistochemistry. The expression levels of thyrotropin-releasing hormone (TRH), prostacyclin (PGI2) and gangliosides (GM) were measured using an enzyme-linked immunosorbent assay. PDXK was identified as the target gene of miR-124. The overexpressed miR-124 group exhibited higher miR-124 expression than the SCI, NC and si-PDXK groups. Compared with the SCI and NC groups, the PDXK expression was downregulated in the overexpressed miR-124 and si-PDXK groups, and the BBB scores were significantly increased 7, 21 and 35 days after transplantation. The double-labeled positive cell densities (Brdu+NSE/NF/MAP2) and the expression levels of TRH, PGI2 and GM in the overexpressed miR-124 group were significantly higher than those in the NC and SCI groups. These results indicated that miR-124 targeted PDXK to accelerate the differentiation of BMSCs into neurocytes and promote SCI repair.