Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

기술수준에 따른 여자축구 인프론트 킥 동작의 협응구조

지보경,육동원,김지수,신정택 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

The purpose of this study was to investigate coordination structure of infront kick in women soccer as a function of skill level. To succeed this purpose, four girls' high school students who had never experienced soccer before were selected as novices, and four girls' high school students who had played soccer for over three years as experts in this lab. To recognize the change of coordination structure according to practice, novices had practiced infront kick in soccer for two weeks. consequently they were taken films of kicking motion three times -before practice, after one week practice, after two weeks practice. However, experts were taken once. The experimental instruments for this study consisted of a video camera, reference frame. LED and APAS for digitizing. Angle to angle diagrams and phase plan portraits for qualitative analysis of coordination structure were gotten with LabVIEW 6i. The conclusions are as follow: firstly, novices' coordination structure had been changed since practice. Secondly, there were significantly different between coordination structures of experts and pre-practice novices. Lastly, there were similar between coordination structures of experts and post practice novices.

진단 기준에 따른 취학전 어린이의 중증 유아기 우식증 유병률 비교

임경욱,이광희,라지영,이동진,안소연,김지영,송지현,김윤희 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.4

연구목적은 중증 유아기 우식증에 대한 두 개의 정의를 진단 기준으로 하였을 때 각 기준에 따른 유병률이 일치하는 정도를알기 위함이었다. 연구대상은 익산시 어린이집 중에서 무작위로 추출한 7개 어린이집의 36개월 이상 71개월 이하 어린이401명 이었다. 치경과 탐침을 사용한 구강검사를 통하여 치아별 우식경험유치면 (dmfs)의 수를 조사하였으며 . 중증 유아기 우식증의 진단 기준은 (1) 상악 전치에 1개 이상의, 와동이 형성된 우식,상실(우식증에 의한), 충전 평활면이 있거나 (2) 우식,상실,충전 치면의 수가 3세는 4개 이상.4세는 5개 이상.5세는 6개 이상인 경우로 하였다. 상악 전치부 우식증에 의한 우식유병률과 우식경험유치면수(dmfs)에 의한 우식유병률은 3세에서 각각 26.7%. 28.9%, 4세에서 각각 32.4%, 30.4%, 5세에서 각각 39.4%, 41.3%, 전체에서 각각 35.4%, 35.9%으로서. 연령별로는 약간의 차이가 있었으나 전체에서는 차이가0.5%로서 거의 일치하였다. 따라서 중증 유아기 우식증의 진단은 두 가지 기준 중에서 어느 하나를 사용하더라도 비슷한 결과가 나을 것이라는 것을 확인할 수 있었다. The purpose of study was to compare the prevalences of severe early childhood cahes(S-ECC) using two different diagnostic criteria. The subjects of this study included 401 preschool children from 36 to 71 months of the randomly selected seven nurseries in Iksan city. Severe early childhood caries was defined as (1) 1 or more cavitated, missing (due to caries), or filled smooth surfaces in primary maxillary anterior teeth or (2) a decayed, missing, or filled score of ≥4 (age 3), ≥5 (age 4), or ≥6 (age 5) surfaces. The prevalence determined by maxillary anterior caries and the prevalence determined by dmfs were 26.7%, 28.9% in 3 years, 32.4%, 30.4% in 4years. 39.4%, 41.3% in 5 years, and 35.4%, 35.9% in all, respectively. The results suggest that the two diag-nostic criteria of the severe early childhood caries yield almost the same results.

통합체육에 대한 장애인의 인식

이경준,김지태 한국특수체육학회 2005 한국특수체육학회지 Vol.13 No.1

The purpose of this study was to throughly identify the idea on inclusive physical education from the perspectives of people with disabilities. In order to accomplish this, critical ethnography was used. The key informants were the students(n=12) of Y University in Seoul, the members of the human rights society group for people with disabilities, 'Guernica'. The data were collected through the participant observation, in-depth-interview, unstructured interview and document analysis. The data were interpreted through the qualitative research procedures which are transcription, coding, categorization and conceptualization. The findings could be summarized as follows: First, although people with disabilities were treated inappropriately, they had positive attitudes on inclusive physical education and believed that it could reduce the bias toward people with disabilities. Second, the people with disabilities perceived inclusive physical education as a sport itself, not as a rehabilitation. Third, the precondition for the best inclusive physical education needs for the reform of consciousness in both people with and without disabilities.

결정형 규산분진에 의한 A549 세포의 독성 및 섬유모세포 증식에 Desferrioxamine이 미치는 영향

김경아,김은경,김지홍,임영 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.2

The inhalation of crystalline silica results in the production of reactive oxygen species (ROS). Among these ROS, hydroxyl radical (˙OH) is believed to be the most reactive one. ˙OH is generated in reaction between superoxide and hydrogen peroxide catalyzed by transition metal, especially iron. Therefore iron should be important in the bioactivity of crystalline silica. Desferrioxamine, a iron chelator, may be protective in silica-induced pulmonary reaction. To test this assumption we investigated the protective effect of desferrioxamine on lipid peroxidation of cell membrane, cytotoxicity, production of proinflammatory and chemotactic cytokine and fibroblast proliferation by crystalline silica in vitro model. The results were as follows; 1. Fenton activity of silica and asbestos was significantly higher than that of control. Fenton activity in crocidolite was higher than silica at the same dose. This result correlated with iron content of dust. Fenton activity of silica and crocidolite was decreased by preincubation of silica with desferrioxamine. 2. Silica induced a dose-dependent increase of MDA concentration in lung epithelial cell lysate dose dependently. Marked decrease of MDA was observed in desferrioxamine treated silica group compared with untreated group. 3. As concentration of stimulated silica, silica-induced cytotoxicity was increased. There was significant decrease of cytotoxicity in desferrioxamine-treated silica group compared with untreated group. 4. α-quartz augmented the production of TNF-α and IL-8 from A-549 cell. While desferrioxa-mine suppressed the release of cytokines. 5. Supernatant of silica-cocultured A549 cell induced a significant proliferation of fibroblast, which desferrioxaime blocked this proliferation. From these result, we concluded that desferrioxamine has a protective effect on silica induced pulmonary reaction.

정신분열병 환자의 인지기능 상태

이지혜,오소영,윤정흠,김선경,이귀행 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.1

목적: 정신분열병 환자의 적응 상태와 증상 형성에 영향을 주는 것으로 생각되는 인지기능 상태를 주의력, 형태 인식, 단어에 대한 구별과 종합 기능을 평가해봄으로써 알아보고자 하였다 방법: 정신분열병 환자 27명과 대조군 27명을 대상으로 하였고, 평가 도구로써 주의력은 K-WAIS의 숫자외우기 문제로, 형태 인식은 K-WAIS의 모양 맞추기 문제와 숨은 그림 찾기로, 단어 기능은 K-WAIS의 공통성 문제와 단어 차이 검사로 각각 이루어졌다. 통계 처리는 SPSS 11.5를 이용하여 Chi-Square test와 Student's t-test로 검증을 하였다. 결과: 정신분열군은 숫자외우기, 숨은 그림 찾기, 모양 맞추기에서는 대조군보다 낮은 점수를 보였다. 단어 차이 검사와 공통성 문제에서는 양군에서 차이를 보이지 않았다. 결론: 본 연구에서 정신분열군의 인지 기능은 정상인 대조군에 비해 주의력과 형태 인식 기능이 유의하게 떨어져 있었으며, 단어들을 구분하고 종합하는 능력은 차이가 없었다. 이런 결과는 임상적으로 정신분열병에서 보이는 망상이 언어적인 측면에서는 어느 정도 전달이 되는 현상과 일치하지만 주의나 형태인지와 같은 기본적인 과정에서 장애가 있다는 추정을 하게 한다. Purpose: This study aimed to investigate the cognitive function in patients with schizophrenia by assessing attention, recognition of a shape, and verbal function. Methods: The subjects were 27 patients with schizophrenia group and 27 control group. Attention was assessed by the digit span task(K-WAIS subtest). The recognition of a shape was assessed by the object assembly task(K-WAIS subtest) and hidden figure task. Verbal function was assessed by Similiarities(K-WAIS subtest) and the different word task. Analysis was performed by the Chi-Square test and Student's t-test via SPSS 11.5. Result: Schizophrenia group had statistically lower scores than control group in digital span, hidden figure and object assembly. There was no difference between two groups in dimensions of difference in words and similarity. Conclusion: This study showed that patients with schizophrenia had impairments in attention and recognition of shape, and no difference between two groups in verbal ability. These explained the partial communicative function of delusion in verbal level. The formation of delusion might be related to the more basic elements such as attention and recognition of shape.

사회적 상호작용을 강조한 과학 탐구실험의 효과

김지영,성숙경,박종윤,최병순 한국과학교육학회 2002 한국과학교육학회지 Vol.22 No.4

사회적 상호작용을 강조한 과학탐구실험이 학생들의 학업성취와 과학탐구능력 및 학습동기에 미치는 효과에 대해 연구하였으며, 탐구실험 수업이 소집단의 구성방식에 따라 어떠한 차이를 나타내는지 알아보기 위해 인지수준이 동질인 집단과 이질인 집단의 효과를 비교하였다. 이를 위해 중학교 2학년 255명을 통제집단과 실험집단으로 나누어, 실험집단에는 사회적 상호작용을 강조한 과학탐구실험 수업을, 통제집단에는 전통적인 실험 수업을 1년간 적용한 후 과학학업성취도, 과학탐구능력, 학습동기 검사를 실시하였다. 사회적 상호작용을 강조한 탐구실험은 이끌어내기, 친숙해지기, 구성하기, 확장하기의 4단계로 구성되어 있으며, 관찰 현상을 학습과 연관시키고 실험을 설계하며 결론을 이끌어내는 과정을 통해 동료 및 교사와 상호작용을 할 수 있도록 구성되어 있다. 연구 결과 사회적 상호작용을 강조한 과학탐구실험은 학생들의 학업성취와 과학탐구능력 및 학습동기에서 유의미한 효과가 있었다. 모든 인지수준의 학생들에게 효과가 나타난 것은 아니었으나 대분분의 인지 수준에서 학업성취의 효과가 크게 나타났으며, 학업성취에 비해 과학탐구능력의 향상정도가 낮았다. 소집단 구성에 따른 효과에서는 동질집단이 이질집단에 비해 성취도와 학습동기에서 유의미한 향상이 있었다. 이러한 결과는 과학탐구실험에서 상호작용이 중요하며, 상호작용이 학생들의 성취도와 탐구능력 향상에 긍정적 영향이 있음을 시사한다. This study investigated the effects of scientific inquiry experiments emphasizing social interaction on the academic achievement, the ability for science inquiry and the learning motivation of the students. To examine the differences among the classes of scientific inquiry experiments according to the way of organizing small groups, the effects of the group in homogeneous cognitive level and the group in heterogeneous cognitive level were compared. 255 7th-graders were grouped into the treatment group and the control group. After the treatment group received the scientific inquiry experimental lessons emphasizing social interaction and the control group received traditional lessons for one year, academic achievement, ability for scientific inquiry, and learning motivation were examined. These inquiry experiments were composed of four steps which are eliciting, familiarizing, constructing and bridging. And students can interact with peers and teachers through the process of relating observed phenomena to concept, constructing experiment procedure, and eliciting results. The ANCOVA results revealed that there were significant effects of science inquiry experiments emphasizing social interaction on the academic achievement, the ability for scientific inquiry and the learning motivation of the students. The significant academic achievement of the students in most of the cognitive levels, if not all, was found and degree of improvement in the ability for scientific inquiry was lower than that in the academic achieve3ment. Concerning the effects according to the way of organizing small groups, there were significant improvement in academic achievement and learning motivation of the group in homogeneous cognitive level compared to the group in heterogeneous cognitive level. These results indicate that social interaction in learning is important and it has positive influences on the improvement in academic achievement and ability for scientific inquiry of students.

불안정 지지면을 이용한 운동 프로그램이 뇌졸중 환자의 균형에 미치는 효과

이지연,김용수,김진섭,박진현,최윤희,전덕훈,김경 대구대학교 특수교육재활과학연구소 2010 再活科學硏究 Vol.28 No.1

본 연구의 목적은 뇌졸중 환자의 재활을 위하여 불안정 지지면을 이용한 운동 프로그램이 균형 능력과 운동 기능 능력에 미치는 효과를 알아보고자 실시하였다. 불안정 지지면의 이용한 치료는 임상적으로 뇌졸중으로 인한 편마비 환자뿐만 아니라 스포츠 손상 및 노인들의 질환 등 다양한 병인을 가진 환자들의 치료에 이용되고 있다. 대부분의 뇌졸중 환자들은 재활 훈련을 할 수 있는 병원이나 기관에서 치료를 받을 때에 지지면의 차이를 제공함으로써 보다 더 안정한 지지면에서 적응할 수 있는 방법이 요구되고 있다. 하지만, 아직도 불안정 지지면을 이용한 구체화된 치료적 운동 방법과 객관적 평가에 대한 연구가 미비한 실정이다. 이러한 문제점을 해결하기 위하여 최근 뇌졸중 환자의 불안정 지지면과 이론적 근거를 바탕으로 한 운동 프로그램에 대한 연구가 더욱 필요하다. 본 연구에서는 실험군과 대조군이 같은 운동 프로그램을 적용하였을 때, 두 그룹 간에서 나타나는 균형 향상과 운동 기능 향상에 어떠한 효과를 주는지 살펴보고자 하였다. The purpose of this article was to study the effect of the exercise programme that uses the unstable supporting surface to rehabilitate cerebrovascular patients on balance ability and exercise function ability. The therapy is being clinically used for the patients with diverse pathological factors such as hemiplegia caused by CVA, sports injury, and diseases in the elderly. when most patients with CVA are treated in the hospital or institute that can provide rehabilitation training, they need to be given the way to adjust more stable surpporting surface by being provide with various stages of surface. However, the actual therapeutic exercises is still needed and the study for objective assessments wants conducting. To solve these problems, the study of unstable surface exercise programmes based on the evidences recently need to be made for the patients with CVA. in this study, we intend to look into what effects on the balance improvement and the exercise function are different from the experimental group to the control group with the same programme applied to the two groups.

신경망을 이용한 공정 오차 보정

전경아,유지용,오혜근 한양대학교 이학기술연구소 2002 이학기술연구지 Vol.5 No.-

패턴의 미세화에 따른 정확하고 신속한 결과 예측은 Iithography simulation tool 의 높은 공정 변수 의존을 요구한다. 이로 인해 simulation tool 은 공정 변수 사이의 보정이 더욱 복잡해져 광 근접 효과 등으로 나타나는 다양한 패턴들의 크기 결과를 예측하는데 많은 어려움을 겪게된다. 이에 본 논문에서는 simulation 결과 값과 실측값의 차이를 신경망 알고리즘에 적용하여 공정 변수를 최적함으로서 simulation tool의 예측 정확성을 향상시키는 방법을 제시하였다. It is required to make an accurate and quick prediction of critical dimension (CD)for higher integrated devices. Because simulation tools consist of many process parameters and models, it is difficult that process parameters are calibrated to match the CD results for various patterns. This paper presents a method of improving the accuracy of predicting CD results by applying △ (the difference between simulation and experimental data) value to neural network algorithm (NNA) in order to reduce the CD difference caused by optical proximity effects.

갑상선 기능항진증에 병발된 주기성 마비 3예

최지훈,최경숙,김기훈,박병현,조정구 圓光大學校 醫科學硏究所 2001 圓光醫科學 Vol.16 No.1-2

Thyrotoxic periodic paralysis is characterized by intermittent flaccid paralysis of skeletal muscle, lasting several hours. The underlying pathophysiology of the syndrome is not yet to be well characterized and remains controversal, but reflects altered potassium and calcium dynamics as well as certain morphologic characteristics within the muscle unit itself. It occurs primarily in Asian populations with an overwhelming male preponderance and a higher association of specific HLA antigens. Precipitating factors include high carbohydrate ingestion, exercise, trauma, infection, alcohol ingestion, administration of epinephrine, thyroid hormone, or corticosteroid. If hypokalemia is present, potassium administration may help abort the attack, but the only definitive treatment is formation of euthyroid state. We discribed two patients with hypokalemic thyrotoxic periodic paralysis and one patient with normokalemic thyrotoxic periodic paralysis.