[PB-0011] Development of multi-stress tolerant temperate rice variety using multi-omics breeding

Zilhas Ahmed Jewel(Zilhas Ahmed Jewel ),Ian Paul Navea(Ian Paul Navea ),Jae Hyuk Han(Jae Hyuk Han),Na-Hyun Shin(Na-Hyun Shin),Joong Hyoun Chin(Joong Hyoun Chin) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Perceptions of UP Los Baños Agribusiness Management Graduates on Their Job Preparedness

Jewel Joanna S. Cabardo,Jeanette Angeline B. Madamba 한국무역연구원 2014 The International Academy of Global Business and T Vol.10 No.2

This study primarily assessed the Bachelor of Science in Agribusiness Management (BSABM) degree program of UP Los Baños (UPLB) through its graduates’ perceptions of their readiness on the job front. Specifically, the study focused on ascertaining the career paths taken by the graduates after attaining their degrees, identifying which subjects/skills the graduates perceived as having contributed to their job preparedness, determining the factors affecting the graduates’ perception of their job readiness and recommending changes for the improvement of the BSABM curriculum based on suggestions obtained from the respondents. Data collection was done through a survey of 150 BSABM alumni selected through purposive sampling. Results from descriptive data analysis indicated that career paths, demographic characteristics, parameters pertaining to college life and job satisfaction indicated the respondents’ preparedness for their jobs. Perceived gaps in their training by the graduates were recognized to suggest changes for the improvement of the BSABM curriculum.

Characterization and stress induced expression profiling of MLO family genes in Cucumis melo L.

Jewel Howlader,Jongin Park,Nasar Uddin Ahmed,Jeong suk hyeon Han,Hee-Jeong Jung,Ill Sup Nou 한국원예학회 2015 한국원예학회 학술발표요지 Vol.2015 No.5

Expression Profiling of MLO Family Genes under Podosphaera xanthii Infection and Exogenous Application of Phytohormones in Cucumis melo L.

Jewel Howlader(쥬엘 하울라다),Hoy-Taek Kim(김회택),Jong-In Park(박종인),Nasar Uddin Ahmed(나잘우딘 아메드),Arif Hasan Khan Robin(아리프 핫산 칸 로빈),Hee-Jeong Jung(정희정),Ill-Sup Nou(노일섭) 한국생명과학회 2016 생명과학회지 Vol.26 No.4

멜론 흰가루병(Podosphaera xanthii)은 멜론 생산량에 영향을 미치는 중요한 병해 중 하나로 알려져 있다. 작물육종에 있어서 흰가루병을 포함한 병저항성 계통 육성은 병저항성 관련 유전자들의 유전양식 및 그 유전자들을 조절하는 식물호르몬에 대한 정보는 매우 중요하다. 식물에 있어 흰가루병균 저항성에 관여한다고 알려진 Mildew Resistance Locus O (MLO) 유전자를 멜론 database인 ‘Melonomics’으로부터 14개 동정하여 CmMLO1~14 (Cucumis melo MLO)로 표기하였다. 동정된 14개의 CmMLO유전자들의 아미노산 서열을 비교한 결과, 9개의 CmMLO 유전자들은 흰가루병균에 대한 감수성 관련 아미노산 cysteine과 proline이 잘 보존되어 있었지만, 나머지 CmMLO 유전자들은 다른 아미노산 서열을 가지고 있었다. 멜론 흰가루병의 7 race에 대하여 이병성을 나타내는 멜론 계통‘SCNU1154’에 멜론 흰가루병균(P. xanthii)을 접종하고, 식물호르몬(metyl jasmonate와 salicylic acid) 처리한 후 qPCR을 통해 CmMLO 유전자들의 상대적인 발현양을 분석한 결과, 멜론 흰가루병 7 race에 대하여 14개의 CmMLO 유전자들 중 3개의 유전자들에서 발현이 증가하였고, 7개의 유전자들은 발현이 감소하였으며, 4개의 CmMLO 유전자들은 race 특이적으로 발현양이 증가 혹은 감소하였다. 또한 14개의 CmMLO 유전자들의 발현양은 methyl jasmonate와 salicylic acid를 처리하였을 때 다양한 발현 양상을 나타내었다. 11개의 CmMLO 유전자들은 salicylic acid 처리하였을 때 발현양이 증가하였으며, 7개의 유전자들은 methyl jasmonate 처리하였을 때 발현양이 증가하였다. 이와 같이, 스트레스에 반응을 보이는 CmMLO 유전자들은 멜론 흰가루병 저항성 계통 육성을 위한 유용한 정보가 될 것으로 기대된다. Powdery mildew disease caused by Podosphaera xanthii is a major concern for Cucumis melo production worldwide. Knowledge on genetic behavior of the related genes and their modulating phytohormones often offer the most efficient approach to develop resistance against different diseases. Mildew Resistance Locus O (MLO) genes encode proteins with seven transmembrane domains that have significant function in plant resistance to powdery mildew fungus. We collected 14 MLO genes from ‘Melonomics’ database. Multiple sequence analysis of MLO proteins revealed the existence of both evolutionary conserved cysteine and proline residues. Moreover, natural genetic variation in conserved amino acids and their replacement by other amino acids are also observed. Real-time quantitative PCR expression analysis was conducted for the leaf samples of P. xanthii infected and phytohormones (methyl jasmonate and salicylic acid) treated plants in melon ‘SCNU1154’ line. Upon P. xanthii infection using 7 different races, the melon line showed variable disease reactions with respect to spread of infection symptoms and disease severity. Three out of 14 CmMLO genes were up-regulated and 7 were down-regulated in leaf samples in response to all races. The up- or down-regulation of the other 4 CmMLO genes was race-specific. The expression of 14 CmMLO genes under methyl jasmonate and salicylic acid application was also variable. Eleven CmMLO genes were up-regulated under salicylic acid treatment, and 7 were up-regulated under methyl jasmonate treatments in C. melo L. Taken together, these stress-responsive CmMLO genes might be useful resources for the development of powdery mildew disease resistant C. melo L.

Development of powdery mildew race 5-specifi c SNP markers in Cucumis melo L. using whole-genome resequencing

Jewel Howlader,Yeji Hong,Sathishkumar Natarajan,Kanij Rukshana Sumi,Hoy-Taek Kim,Jong-In Park,Ill-Sup Nou 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.2

Melon ( Cucumis melo L.), belonging to the Cucurbitaceae family, is cultivated worldwide and is highly valued for its fruitquality. However, this important crop is negatively aff ected by several races of powdery mildew (PM) fungus, Podosphaeraxanthii . Hence, exploration of PM race-specifi c resistant markers would be an eff ective strategy to develop race-specifi cmelon cultivars. Young leaves from four melon genotypes, including susceptible SCNU1154 and race-specifi c-resistantEdisto47, PMR5, and MR1 lines, were sequenced by next-generation sequencing, specifi cally whole-genome resequencing(WGR), to detect race-specifi c single nucleotide polymorphism (SNP) markers. Including putative resistance gene ( R -gene)SNPs, 168, 83, and 122 race 5-specifi c SNPs with the exact variation were identifi ed on PM-related chromosome 2, 5, and12, respectively, for distinguishing race-specifi c lines by comparing the WGR data in the C. melo genome. Based on proximityto the PM resistance quantitative trait loci (QTLs) in physical maps, 43, 37, and 48 SNPs were screened on chromosome2, 5, and 12, respectively. Using a derived cleaved amplifi ed polymorphic sequence (dCAPS) method, polymorphismswere only found in three SNPs (SNPR5_119, SNPR5_120, and SNPR5_121) out of 48 against race 5 on chromosome 12. Among these three, a putative R -gene, an NBS-LRR-type SNP, SNPR5_119, displayed similar genotypic and phenotypicvariation to race 5-specifi c susceptible (SCNU1154, PMR45, WMR29, and Edisto47) and resistant (PI414723, PMR5, andMR1) lines in C . melo except for PI124112. Importantly, two other intergenic SNPs, SNPR5_120 and SNPR5_121, showedgenotypic and phenotypic variation between susceptible and resistant lines tested. High-resolution melting (HRM) analysiswas used to further validate the same expression patterns of SNPR5_120 as a dCAPS method in all lines tested. Therefore,the identifi ed PM race 5-specifi c candidate markers described here might be useful for a marker-assisted selection breedingprogram in C. melo .

The Ties that Bind in The Elder Statesman

Jewel Spears Brooker 한국T.S.엘리엇학회 2010 T.S. 엘리엇 연구 Vol.20 No.2

A major theme of Eliot’s work is the difficulty of genuine and lasting human connection. Connection can be based on a positive (love) or a negative (guilt). The thesis of this essay is that certain shared actions constitute a tie that binds―especially shared actions that have been concealed, secrets kept between two guilty parties. Complicity in such actions binds partners together socially, psychologically, and morally. “Friendships” based on shared secret sins last for a lifetime; they can be denied, but they cannot be nullified, and finally, they must be acknowledged in public. In The Elder Statesman, crime, sex, and blood (family) are the ties that bind.

Eliot’s Exilic Imagination

Jewel Spears Brooker 한국T.S.엘리엇학회 2015 T.S. 엘리엇 연구 Vol.25 No.3

T. S. Eliot, like Conrad, Joyce, Pound, Beckett, and other greats of his generation, had an exilic imagination. Displaced from the country of their birth, they were conscious of being strangers in an uneasy world. This essay outlines Eliot’s sense of displacement and homelessness from 1914 to the late 1950s, with emphasis on the effect that the impulse to return had on his major poems. The Waste Land is a poem of personal and cultural exile, Ash-Wednesday is an ode expressing both the pain of exile and the hope of return, and Four Quartets is a composition that reconfigures exile as a life-long spiritual odyssey.

What T. S. Eliot Learned from Dante

Jewel Spears Brooker 한국T.S.엘리엇학회 2011 T.S. 엘리엇 연구 Vol.21 No.2

Dante is one of those poets, Eliot once said, that one only grows up to at the end of one’s life. In Eliot’s work, growing up to (learning from) his Italian master has three primary dimensions. The first is psychological, and it has to do with a perception, in reading, of feelings and ideas as unified. The second is aesthetic, and it has to do with poetry, with understanding how to achieve such unification in art. And the third is moral, and it has to do with social and spiritual unification through the cultivation of humility.

The War Within: Feeling and Intellect in Early Eliot

Jewel Spears Brooker 한국T.S.엘리엇학회 2009 T.S. 엘리엇 연구 Vol.19 No.2

T. S. Eliot’s career began with a struggle between poetry and philosophy. The agon featured Dante, whose work informed Eliot’s earliest poems, and F. H. Bradley, whose thought was the subject of his Ph.D. thesis. Eliot’s most detailed discussion of the connection between poetry and philosophy is contained in his 1926 Clark Lectures at Cambridge University, published as Varieties of Metaphysical Poetry. He defines the “philosophic poet” in Bradleyean terms as one who “enlarges immediate experience” by “drawing within the orbit of feeling and sense what had existed only in thought” (VMP 55,51). Philosophic poetry is work of the “highest intensity, in which the thought is fused into poetry at a very high temperature” (VMP 50). Eliot argues that Dante’s poetry perfectly illustrates the integration of feeling and intelligence, both in life and in art. In this paper, I explore Eliot’s attempt to negotiate the claims of philosophy and poetry, as represented by Bradley and Dante, and his ultimate decision to abandon a promising career in philosophy for a tenuous career in poetry. Eliot’s ambition of becoming a “philosophic poet,” combining Bradley and Dante, was realized in his Dantean sequences Ash-Wednesday and Four Quartets.

Characterization and Expression Analysis of Peroxidases and Glucan Synthase Like Genes in Cucumis melo L.

( Jewel Howlader ),( Kanij Rukshana Sumi ),( Hoy Taek Kim ),( Arif Hasan Khan Robin ),( Jong In Park ),( Mi Young Chung ),( Ill Sup Nou ) 한국육종학회 2016 Plant Breeding and Biotechnology Vol.4 No.2

Powdery mildew (PM) is a severe fungal disease for melon cultivation worldwide. Stress resistance related genes could be important tools to address this problem. In this study, we retrieved defense related peroxidase and glucan synthase genes from Melon Genome Database ``Melonomics``. Thereafter, we analyzed the genes in silico. We conducted protein blast in the NCBI database and found a high degree of homology among them. Based on the highest protein homology we named two isoforms of Cucumis melo peroxidase 2-like genes (CmPrx2-1 and CmPrx2-2) and one glucan synthase1-like gene (CmGLS1). In reverse transcriptionpolymerase chain reaction (PCR), all 3 genes showed organ specific expression in a C. melo line, SCNU1154. Real-time quantitative PCR expression of these 3 genes was conducted in the infected leaf samples by PM fungus Podosphaera xanthii and also treated leaf samples by exogenous phytohormones (salicylic acid and methyl jasmonate). The CmPrx2-2 gene was up-regulated in response to all seven races of PM fungus whereas up-regulation or down-regulation of CmPrx2-1 gene was race-specific. The CmGLS1 gene was down-regulated in response to all races except one race. The CmPrx2-1, CmPrx2-2, and CmGLS1 genes were up-regulated under both salicylic acid and methyl jasmonate treatments but their level of expression was higher in salicylic acid treated plants compared to methyl jasmonate. Therefore, we speculate that defense response of the three tested genes is largely mediated by the salicylic acid signaling pathway under PM infection. Taken together, the data presented herein may be useful resources in the development of PM stress resistant in C. melo L.