Zn과 Cd의 처리가 GA와 Uniconazole 전처리된 메리골드 'Orange Boy'의 생육과 무기물 흡수에 미치는 영향

정성우,정연옥,허무룡,박중춘 경상대학교 농업생명과학연구원 2002 농업생명과학연구 Vol.36 No.4

메리골드(Tagetes patula L. 'Orange Boy')를 통해 오염지 식생복원과 동시에 양호한 식생경관 조성재료로서의 가능성을 알아보기 위해 본 실험을 수행하였다. 메리골드 'Orange Boy'에 있어서 Zn과 Cd 100+20㎎·L^-1 처리구에서는 GA_3 와 uniconazole은 생체량 및 Zn과 Cd의 축적에는 영향을 주지 않았다. 생장조절제 무처리구의 Zn과 Cd의 200+40㎎·L^-1 처리구에서 대조구의 20%로 건물량이 감소하였다. Zn과 Cd의 농도별 혼용처리에 따른 체내 무기성분 T-N, P, K, Ca, Mg함량은 뚜렷한 경향이 없었고, Zn의 축적은 뿌리와 잎에서 각각 1,227 및 903㎎·㎏^-1, Cd의 경우 줄기와 잎에서 각각 89.7 및 77.4㎎·kg^-1의 최대의 농도를 보였다. The use of plant-based systems to remediate contaminated soils has become an area of intense scientific study in recent years and it is apparent that plants which grow well in contaminated soils need to be identified and screened for use in phytoremediation technologies. This study was conducted to determine the effects of various combinations of Zn and Cd on growth and mineral concentrations of Tagetes patula L. 'Orange Boy'. With 100㎎·L^-1 Zn + 20㎎·L^-1 Cd, GA_3 and Uniconazole did not increase the plant biomass, and accumulation of Zn and Cd Total dry weight with 200㎎·L^-1 Zn and 40㎎·L^-1 Cd was 20% that of control. There was no significant to T-N, P, K, Ca, and Mg contents in leaf, stem, and root. The Zn contents of root and leaf were 1,227 and 903 ㎎·kg^-1, respectively. While the Cd was accumulated different site compared to Zn, the highest accumulation place were stem (74.4 ㎎·kg^-1) and leaf (89.7 ㎎·kg^-1).

척추 주위근의 단면적과 등척성 요추부신전 근력간의 상관 관계

권정이,이강우,김현숙,김종문,안중모 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.2

치과용 디지털 색상 분석용 기기의 정확성과 재현 능력에 대한 평가

정중재,박수정,조현구,황윤찬,오원만,황인남 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.4

본 연구에서는 치과용 디지털 색상 분석기기 중 하나인 ShadeScan (CYNOVAD, Montreal, Canada)의 색상 분석의 정확성과 재현 능력을 평가하였다. 균일한 치아 배열을 가진 20대 성인 남자 8명과 여자 8명의 상악 인상을 고무인 상제 (Exaflex, GC, Japan)를 이용하여 채득하고, 인상에 A2 색상의 임시 크라운 제작용 자가중합형 레진을 주입하여 치아 모델을 완성하고, ShadeScan을 이용해 각 치아의 영상을 얻어 컴퓨터의 ShadeScan 프로그램으로 분석하였다. 그리고 측정 결과의 재현 능력를 평가하기 위해 2개의 치아 모델을 임의로 선택하여 각 치아마다 10회의 색상 측정을 시행하여 분석 결과를 비교하였다. 또한 분석된 영상의 동일 shade 간의 색차를 확인하기 위해 광중합 복합레진인 Gradia Direct (GC, Tokyo, Japan)와 shade guide의 CIE L^(*)a^(*)b^(*) 값을 백색과 흑색 배경판 상에서 spectrophotometer (Spectrolino, GretagMacbeth, USA)로 측정하고, ShadeScan에 의해 동일한 shade로 분석된 shade guide간의 색차를 계산하여 다음의 결과를 얻었다. 모든 치아에서 모델 제작 재료와 동일한 A2 색상므로 분석된 경우는 없었으며, 개개의 치아에서 전체 순면이 동일한 색상으로 분석된 예는 없었다. 또한 동일한 치아군에서 치아의 크기 , 형태 , 순면의 굴곡 등에 따라 서로 다른 색상 분포를 보였다. 재현성 평가에서 각 치아의 중앙부는 비교적 일정한 재현성출 보였으나, 절단부와 주변 부위는 재현성이 낮았으며, 동일한 shade로 분석된 shade guide 간에는 3 이상의 색차 (ΔE^(*))를 보였다. 이상의 결과는 치과용 디지털 색상 분석기기는 비록 높은 재현 능력은 가지고 있지만 정확성에 오류가 있음을 시사하며 , 따라서 이러한 기기를 이용한 색상 분석 시 좀 더 세밀한 주의와 다른 방법의 추가 사용이 필요하리라 사료된다. This study was done to evaluate the reliability of the digital color analysis system (ShadeScan, CYNOVAD, Montreal, Canada) for dentistry. Sixteen tooth models were made by injecting the A2 shade chemical cured resin for temporary crown into the impression acquired from 16 adults. Surfaces of the model teeth were polished with resin polishing cloth. The window of the ShadeScan handpiece was placed on the labial surface of tooth and tooth images were captured, and each tooth shade was analyzed with the ShadeScan software. Captured images were selected in groups, and compared one another. Two models were selected to evaluate repeatability of ShadeScan, and shade analysis was performed 10 times for each tooth. And, to ascertain the color difference of same shade code analyzed by ShadeScan, CIE L^(*)a^(*)b^(*)values of shade guide of Gradia Direct (GC, Tokyo, Japan) were measured on the white and black background using the Spectrolino (GretagMacbeth, USA), and Shade map of each shade guide was captured using the ShadeScan. There were no teeth that were analyzed as A2 shade and unique shade. And shade mapping analyses of the same tooth revealed similar shade and distribution except incisal third. Color difference (ΔE^(*)) among the Shade map which analyzed as same shade by ShadeScan were above 3. Within the limits of this study, digital color analysis instrument for dentistry has relatively high repeatability, but has controversial in accuracy.

고양이 감각 및 운동겉질에서 calbindin D-28k와 parvalbumin에 대한 면역세포화학적연구

김종중,장인엽,정윤영,윤상필,문정석 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.2

초피나무( Zanthoxylum piperitum D.C.) 種子의 아미노산과 脂肪酸 組成의 分析

鄭宇珪,安重極,成敏雄 경상대학교 과학교육연구소 1996 科學敎育硏究所報 Vol.16 No.1

본 연구는 식용으로 활용할 수 있는 蛋白質源과 油脂源을 얻기 위해 초피나무 종실에 있어 단백질과 지질의 아미노산과 지질의 조성을 분석한 것이며, 아미노산 자동 분석기와 gas liquid chromatography법으로 초피나무 종자의 아미노산과 지방산을 동정한 결과는 다음과 같았다. 종자 단백질에서는 총 104,445.1㎍/g의 아미노산이 함유되어 있었다. 17종류의 아미노산이 분석되었다. 이들 아미노산들 가운데 함량이 많은 아미노산은 아스파르트산 13.18%, 아르기닌 10.06%와 류신 9.34% 등이었다. 총 필수 아미노산은 53,458.0㎍/g 이고 51.18%에 해당하였고 9개의 필수 아미노산이 비교적 많이 함유되어 있었다. 종자의 지방은 건물중량의 30.5%였고, 11종류의 지방산이 분석되었다. 주요 지방산은 oleic acid 37.68%, linoleic acid 15.69%, linolenic acid 4.76% 및 palmitoleic acid 10.90% 등 69.03%를 차지하는 불포화지방산들이었으며, 필수지방산인 linoleic acid와 linolenic acid가 20.45%였다. 이상의 결과들을 볼 때 초피나무의 종자는 가치 있는 蛋白質源과 油脂源이었다. The purpose of this study was to analyze the amino acid composition of protein and fatty acid composition of lipid in the seed of Japanese pepper; Zanthoxylum piperitum D.C. For food protein and oil source, the amino acid and fatty acid composition were determined by the methods of amino acid autoanalyzer and gas liquid chromatography. The results were summarized as follows; Total amino acid of seed protein was 104,445.10㎍/g. Seventeen different kinds of amino acids were analyzed from seed protein. Among them, comparatively high content of asparatic acid(13.18%), arginine(10.06%) and leucine(9.34%) were contained. Total essential amino acid was 53,458.0㎍/g and 51.18%. Among total amino acid, nine different kinds of essential amino acid were contained comparatively high content. The crude oil of seed was 30.5% of dry weight. Eleven kinds of fatty acids were analyzed from the seed lipid. Among them, the main fatty acids were unsaturated fatty acids such as oleic acid(37.68%), linoleic acid(15.69%), linolenic acid(4.76%), palmitoleic acid(10.90%) and etc. These unsaturated fatty acids were 69.03%. Also essential fatty acids such as linoleic acid and linolenic acid were total 20.45% From these results, the seeds of Z. piperitum would be good for protein and oil source.

민초피나무( Zanthoxylum piperitum D.C. var. inerme Makino) 種子의 아미노산과 脂肪酸 助成의 分析

鄭宇珪,安重極,成敏雄 경상대학교 과학교육연구소 1996 科學敎育硏究所報 Vol.16 No.1

본 연구는 최근에 국내에도 자생하는 것으로 알려진 민초피나무의 종자에서 식용으로 활용할 수 있는 蛋白質源 油脂源을 얻기 위해 민초피나무 종실에 있어 단백질과 지질의 아미노산과 지질의 조성을 분석한 것이며, 아미노산 자동 분석기와 gas liquid chromatography법으로 민초피나무 종자의 아미노산과 지방산을 동정한 결과는 다음과 같았다. 종자 단백질에서는 총 92,380.0㎍/g의 아미노산이 함유되어 있었다. 17종류의 아미노산이 분석되었다. 이들 아미노산들 가운데 함량이 많은 아미노산은 아스파르트산 13.95%, 아스파라긴 11.60%와 류신 9.39% 등이었다. 총 필수 아미노산은 47,740.2㎍/g이고 51.68%에 해당하였고 9개의 필수 아미노산이 비교적 많이 함유되어 있었다. 종자의 지방은 건물중량의 29.4%였고, 10종류의 지방산이 분석되었다. 주요 지방산은 oleic acid 33.44%, linoleic acid 14.96%, linolenic acid 6.19% 및 palmitoleic acid 9.50% 등 65.09%를 차지하는 불포화지방산들이었으며, 필수지방산인 linoleic acid와 linolenic acid가 21.15%였다. 이상의 결과들을 볼 때 초피나무의 종자는 가치 있는 蛋白質源과 油脂源이었다. The purpose of this study was to analyze the amino acid composition of protein and fatty acid composition of lipid in the seeds of Zanthoxylum piperitum D.C. var. inerme Makino. For food protein and oil source, the amino acid and fatty acid composition were determined by the methods of amino acid autoanalyzer and gas liquid chromatography. The results were summarized as follows; Total amino acid of seed protein was 92,380.0㎍/g. Seventeen kinds of amino acids were analyzed from seed protein. Among them, comparatively high content of aspartic acid(13.95%), arginine(11.62%) and leucine(9.39%) were contained. Total essential amino acid was 47,740.2㎍/g and 51.68%. Among total amino acid, nine different kinds of essential amino acid were contained comparatively high content. The crude oil of seed was 29.4% of dry weight. Ten kinds of fatty acids were analyzed from the seed lipid. Among them, the main fatty acids were unsaturated fatty acids such as oleic acid(34.44%), linoleic acid(14.96%), linolenic acid(6.19%), palmitoleic acid(9.50%) etc. These unsaturated fatty acids were 65.09%. Also essential fatty acids such as linoleic acid and linolenic acid were total 21.15% From these results, the seeds of Z. piperiturm var. inerme would be good for protein and oil source.

建設에 있어서 經營情報體制 導入에 關한 基礎的 硏究

鄭義容,李重雨,楊淳甲 啓明大學校 産業技術硏究所 1988 産業技術硏究所 論文報告集 Vol.10 No.-

The circumstance of construction are more complicated and more diversified so that the managers may save their money and time in their management. In this regard, this paper will present a basic theory of Total Management information System in construction.

建築見積業務에 對한 電算프로그래밍에 關한 硏究

鄭義容,李重雨,楊淳甲 啓明大學校 産業技術硏究所 1988 産業技術硏究所 論文報告集 Vol.10 No.-

The Computer Programming of Estimation for building construction has been studied with the complicated and diversified circumstance. In spite of many Korea builders attempt to practical use of a computer aid estimation, they have been faced with so many problems in its application. In this regard, this study will present a system for adequate program and estimation method, which is compatible to Korean builders. Science the programme of this computer aided system is composed of standard cost breakdown, bill of quantity, unit price and the estimated cost is to be automatically produced by feeding up the input data.

제2형 당뇨병 환자에서 Voglibose와 Acarbose의 비교임상연구

정인경,정재훈,민용기,이명식,이문규,김광원,정윤이,박중열,홍성관,이기업 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.26 No.2

연구배경:아카보스와 보글리보스는 ­glucosidase inhibitors로써 비록 약리학적 작용이나 부작용에 있어서 두 약물간에 차이가 있다는 것은 잘 알려져 있으나 당뇨병 환자를 대상으로 아직 이에 대한 두 약물간에 직접적인 비교에 대해 연구된 바는 없었다. 이에 저자등은 국내 2형 당뇨병 환자에 대해 유효성과 부작용 발현에 대해 두 약제를 비교하고자 무작위법에 의한 위약 대조군의 이중 맹검법 연구를 시행하였다. 방법:시험 약제 투여 4주간의 관찰기를 설정하여 공복혈당의 변화가 30㎎/dL 이하이고, 식후 혈당이 200㎎/dL 이상인 환자로 기타 제외 기준에 해당하지 않은 환자 53명을 대상으로 하여 보글리보스 군(24명)과 아카보스 군(29명)으로 무작위로 나누었다. 치료기간은 총 8주로 하였으며, 4주간 간격으로 혈청학적 검사와 부작용을 분석하여 치료 효과가 부작용을 평가하였다. 결과:1)혈당 변화:보글리보스군은 식후 1시간 혈당이 치료 후 4주, 8주째 의미 있게 감소하였도, 아카보스군은 식후 1시간과 2시간 혈당이 치료 후 4주, 8주째 의미있게 감소하였다. 또한 관찰기 혈당에 대한 치료 4주째 감소량은 아카보스군에서 더 큰 경향을 보였으나, 치료 8주째에는 두 군 간의 강하정도에 의미있는 차이가 없었다(p=0.569). 2)인슐린 치의 변화:보글리스 군은 식후 1시간 인슐린 치가 치료 전에 비해 치료 4주, 8주째 감소하는 경향을 보였고, 공복 인슐린이나 식후 2시간 인슐린치는 치료전 후에 의미 있는 차이가 없었다. 아카보스군에서는 치료 전후로 공복 인슐린, 식후 1시간과 2시간 인슐린치에 의미 있는 차이를 보이지 않았다. 두약제 간에 치료 전과 치료 8주사이의 식후 2시간 인슐린의 감소량이 보글리보스 군에서 의미있게 높았다(p=0.040). 3)당화혈색소:보글리보스 군은 치료 전에 비해 치료후 당화혈색소가 감소하는 경향을 보였고, 아카보스군은 치료 전에 비해 의미있게 감소하였다. 당화혈색소 변화량은 두 군간에 의미 있는 차이는 없었다(p=0.412). 4)지질대사의 변화:중성지방, 콜레스테롤, 고밀도 진단백 콜레스테롤에 대해 두 군간에 의미 있는 차이는 없었다. 5)부작용:소화기계 부작용의 빈도는 치료 4주째 보글리보스 군에서 의미 있게 낮았으나(p=0.028), 치료 8주째 부작용의 빈도는 두 군간에 의미 있는 차이가 없었다(p=0.215). 결론:2형 당뇨병 환자에서 보글리보스와 아카보스의 두 약제의 임상적 유효성과 부작용발현에 대해 비교한 결과 치료 후 8 주 후 혈당강화효과는 두 약제간에 유사한 효과를 보였으나 보글리보스군에서 4주째의 초기 위장관 부작용이 적었다. Background : Acarbose and voglibose are alpha-glucosidase inhibitors. Although different pharmacological effects and adverse abdominal events associated with the two drugs have been reported, no study directly compared acarbose and voglibose in diabetes has been undertaken. To compare the pharmacological effects and gastrointestinal adverse events between two drugs, a randomized, placebo-controlled, double-bind study was performed in type 2 diabetes patients. Methods : The period of study was 12 weeks(observation period: 4 weeks; treatment period: 8 weeks). Fifty-three patients were randomized into two groups(the acarbose group: 24 patients; the voglibose group: 29 patients). The serum glucose, insulin, fructosamine, HbA_1c, cholesterol, triglyceride and the incidence of adverse events were measured. Results : 1) The reduction of glucose from before treatment to 4 weeks after treatment was significantly higher in the acarbose group, but the change before treatment and 8 weeks after treatment in the two groups was similar(p=0.569). 2) The insulin significantly decreased after voglibose treatment(p=0.040). 3) HbAa_1c level tended to decrease in voglibose group, and there was a significant decrease after acarbose treatment. However, the change in HbA_1c level before and after treatment was similar between the two groups(p=0.412). 4) The two drugs did not cause any other changes in the total, HDL-cholesterol and triglyceride. 5) The number of patients with gastrointestinal adverse events was significantly low 4 weeks after voglibose treatment (p=0.049), but the incidence in the two groups was similar after 8 weeks(p=0.215). Conclusions : Acarbose and voglibose significantly improved postprandial hyperglycemia in diabetes. The incidence of gastrointestinal adverse events was low 4 weeks after voglibose treatment(J Kor Diabetes 26:134~145, 2002).

백서 Paraquat 두여에 의한 간과 폐 조직의 산화성 손상에서 Vitamin C와 Deferoxamine의 항산화 효과에 관한 연구

정연권,서길준,정중식,정성은,최국진,윤여규 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Background: The toxicity of paraquat has been known to be caused by oxygen free radicals which leads to the lipid peroxidation and multiple organ failure. Although vitamin C has been known to be a potent antioxidant, recently there are numerous data which have shown that a low dose of vitamin C may act as a prooxidant due to the stimulation of the Fenton reaction with metal ions, which produces hydroxyl radicals. It has been reported that a deferoxamine in paraquat intoxication could reduce the production of the hydroxyl radicals by the inhibition of the Fenton reaction through the reduction of iron ion in tissue. The aim of this study was to evaluate the effect of the high and low dose of vitamin C and deferoxamine on lipid peroxidation and plasma TNF-α in paraquat intoxication. Methods: Female Sprague -Dawley rats were divided into seven groups: control group which was not given paraquat(20 mg/kg), P group which was given paraquat only, PVH group given paraquat and high dose of vitamin C(100 mg/kg), PVL group given paraquat and low dose of vitamin C(10 mg/kg), PVHD given paraquat, high dose of vitamine C and deferoxamine(100 mg/kg), PVLD given paraquat, low dose of vitamin C and deferoxamine, and PD given paraquat and deferoxamine. Animals were killed at 6 and 24 hours after treatment. Malondialdehyde(MDA), superoxide dismutase(SOD) and glutathione(GSH) contents, catalase activity, plasma TNF-α, and histologic changes in the lung and liver tissue were measured. Results: The lung histology in the PVH and PD or PVHD groups showed the significant decreases in the alveolar edema and interstitial thickness compared to the P group. The liver histololgy in the PVH and PVHD groups demonstrated marked differences in the central venous and sinusoidal dilatation compared to that of the P group. While the MDA levels of the lung and liver in the PVH and PD groups showed the significant reduction compared to that of the P group at 6 hours after treatment, all groups showed the significant changes compared to the P group at 24 hours. There was no significant change of the SOD levels of the lung and liver at 6 hours among all groups. At 24 hours, the SOD levels of the lung in PVH, PVL, and PVHD groups showed the significant increases compared to the P group. The increase of the SOD level in groups combined with deferoxamine, however, revealed a little reduction. The SOD level of the liver in PVH group only significantly increased compared to the P group at 24 hours. There was no significant change of the GSH level of the lung and liver among all groups at 6 hours. At 24 hours, the GSH level of the lung and liver were significantly increased in both PVH and PD group and PVH group, respectively, compared to the P group. Although the catalase activity of the lung was not significantly increased, that of liver was signiflcantly increased in both PVHD and PD groups compared to the P group at 6 hours. The catalase activities of the lung and liver were significantly increased in PVH, PD, and PVHD at 24 hours. The concentrations of the plasma TNF-α were slightly decreased at 6 hours and slightly increased at 24 hours compared to that of the P group, but they were not significant. Conclusion: This study showed that although the low dose of vitamin C had no effect, the high dose of vitamin C revealed a decrease of the MDA level and an increase of SOD, GSH, and catalase activity in the lung and liver tissues, and the effect of the high dose of vitamin C increased with time. The administration of the deferoxamine with or without high dose of vitamin C, however, significantly showed the inhibition of the lipid peroxidation and antioxidant effect and low dose vitamin C decreased the effect of deferoxamine. The effects of the vitamin C and deferoxamine on plasma TNP-α were not clearly shown.