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정미란,차정단,윤순일,한종현,이영은 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.1
Fig(Ficus carica L.) which belongs to Moraceae family has been used as digestion promoter, cure for ulcerative inflammation and eruption in Korea. Figs have very short preservation period and can be kept only 1 week even in the refrigeration system. The possibility of wine-making from Korean figs was tested by analyzing the fermentation characteristics, proximate analysis and free sugar contents of wine made of slices with 20% sugar addition. Ethanol fermentation of the figs by Saccharomyces cerevisiae was done rapidly. The ethanol contents, pH, total acidity and sugar content were 12.1%, 3.91, 0.42% and 9.9 °Brix, respectively. The results from the sensory evaluation were as follows. The wine made from the sliced figs were better than that from fig juices in their sensory scores. The wine made with frozen figs was not different from that made with fresh ones. To improve the quality of wine, the addition of dried leaf powder in the concentration of 100 ppm were tried and found it was superior to the one made without leaf.
정미란,차정단,이영은 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.1
The antibacterial activities of methanol extract and systematic solvent fractions(-hexane, chloroform, ethyl acetate, and butanol) from Korean common type figs at different ripening stages were tested by the broth dilution method against 8 representative food-poisoning bacteria- : L. monocytogenes, S. aureus, S. enteritidis, E. cols 0157:H7, E. cols, Y. enterocolitico, V. parahaemolvticus, and S. ttphimurium. The methanol extracts of unripened Ⅰ and Ⅱ showed stronger activity than that of the ripened figs especially against L. monocytogenes, S. enteriridis, E. toll 0157:H7, V. parahaemolvticus and S. nphimurium in 10 mg/mL. The systematic solvent fractions showed stronger antibacterial activities than the methanol extract, even at the lower concentrations. The hexane fraction of ripened Figs showed higher growth inhibition than those of unripened Ⅰ and Ⅱ against L. monocytogenes, E. colt 0157.117, Y. enterocolitica and V. parahaemolvtieus. The chloroform fraction showed strong antibacterial activity in all ripening stages against E. soli 0157:H7 and V. parahaemolyticus. The butanol fraction showed better inhibition activity in unripened I and II than in the ripened figs. The hexane and chloroform fractions showed inhibition activity of more than 75% against E. coli 0157:117, V. parahaemohvicus in 0.5 mg/mL. Each fraction showed a little different antibacterial activity according to the ripening stages of the fruits and the tested strains. Especially, rigs in the unripened II stage revealed superior activity relatively and the hexane and chloroform tractions revealed the strongest activity, followed by the butanol fraction, while the ethylacetare and water fractions hardly showed any activity.
Potassium alum과 비우식성 감미료의 병용이 Streptococcus mutans의 성장에 미치는 영향
김윤정,차정단,김지영,김강주,이흥수 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.3
Traditionally, Koreans have used potassium alum by mouth rinse when they suffered from stomatitis. This study was carried out to investigate the combined effect of potassium alum with aspartame, sorbitol and xylitol on the growth of Streptococcus mutans (S. mutans). The relative growth ratio of S. mutans JC-2 cultured at different concentrations of potassium alum and different concentrations of aspartame, sorbitol and xylitol was examined. The combined effect of different concentrations of potassium alum with 0.5% and 10.0% of aspartame, sorbitol and xylitol was examined. The lowest minimum inhibitory concentration of potassium alum was 5.0mg/ml against S. mutans JC-2. The relative growth ratio of potassium alum on S. mum JC-2 was affected at the concentration of 2.0mg/ml. The growth of S. mutans JC-2 decreased at the concentration of 10.0% of aspartame and sorbitol. Also, a combination of potassium alum and sugar substitutes was more effective against S. mum than the use of one sugar substitute alone. The inhibitory effect of potassium alum and sugar substitutes on S. mutans may be an important mechanism of caries prevention by potassium alum.
Antimicrobial Activity of Methanol Extract from Ficus carica Leaves Against Oral Bacteria
Jeong, Mi-Ran,Kim, Hye-Young,Cha, Jeong-Dan The Korean Society for Microbiology 2009 Journal of Bacteriology and Virology Vol.39 No.2
Ficus carica L. (fig) belongs to the mulberry tree (Moraceae) which is one of the oldest fruits in the world. It has been used as a digestion promoter and a cure for ulcerative inflammation and eruption in Korea. The present study investigated the antimicrobial activity of methanol (MeOH) extract of figs against oral bacteria. The MeOH extract (MICs, 0.156 to 5 mg/ml; MBCs, 0.313 to 5 mg/ml) showed a strong antibacterial activity against oral bacteria. The combination effects of MeOH extract with ampicillin or gentamicin were synergistic against oral bacteria. We suggest that figs could be employed as a natural antibacterial agent in oral care products.
Jeong-Dan Cha 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.5
Danshen (Salvia miltiorrhiza Bunge) is a traditional Korean medicine that is commonly used for the treatment of inflammatory diseases such as edema, arthritis, hepatitis, and endangitis. The several extracts of danshen were tested for antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) isolated in clinic by broth microdilution method, the checkerboard, and time-kill methods to evaluate the synergistic effects of the combination of the extracts with antibiotics. The chloroform (CHCl₃) and n-hexane (HEX) extracts [minimum inhibitory concentration (MIC), 0.0078-0.3125 ㎍/㎖; minimum bactericidal concentration (MBC), 0.019-0.625 ㎍/㎖] were found to have strong antibacterial activity against MRSA. Additionally, when the CHCl₃ and HEX extracts were co-administered with ampicillin or oxacillin, a synergistic effect against MRSA was observed. Furthermore, a time-kill study evaluating the effects of the extracts against MRSA indicated that treatment with the CHCl₃ extract in combination with ampicillin or oxacillin produced rapid bactericidal activity. These results suggest that danshen extracts may have potently antimicrobial activity and thus, it can be a suitable phytotherapeutic agent for treating MRSA infections.
Jeong-Dan Cha,Youn-Hwa Kim,Ji-Young Kim 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.1
Artemisia lavandulaefolia has been utilized in traditional medicines for the treatment of several diseases. In this study, we attempted to determine whether the essential oil and 1,8-cineole of A. lavandulaefolia induce apoptosis in KB cells. The oil and 1,8-cineole induced the cell death of KB cells as evidenced by the increased cell population in the sub-G1 phase, the appearance of condensed and/or fragmented nuclei, and the generation of a cleaved PARP product. The treatment of the cells with the oil also induced changes in the mitochondrial level of Bcl-2 and Bax, thereby inducing the release of cytochrome c into the cytosol. Furthermore, the oil increased the phosphorylation of extracellular signal-regulated kinase (ERK), c-jun N-terminal kinase, and p38 MAPK, but 1,8-cineole activated pp38 and pJNK at the same concentrations. These findings show that the mitochondrial and MAPKs pathways might be involved in the oil-induced apoptosis,and may enhance our current understanding of the anticancer functions of the oil to a greater degree than 1,8-cineole.
Jeong-Dan Cha,Mi-Ran Jeong,Young-Eun Lee,Kyung-Yeol Lee 한국식품과학회 2007 Food Science and Biotechnology Vol.16 No.4
Sophora flavescens AITON (Leguminosae) is a typical traditional Korean medical herb considered to exhibit antibacterial, anti-inflammatory, and antipyretic effects, and is also used for the treatment of skin and mucosal ulcers, sores, diarrhea, gastrointestinal hemorrhage, arrhythmia, and eczema. In this study, the compound sophoraflavanone G was isolated from the dried roots of S. flavescens by bioassay-guided fractionation. We then investigated the effects of various concentrations of sophoraflavanone G on cell viability and the induction of apoptosis in KB cells after an incubation of 24 hr. The results were determined by the following methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-terazolium bromide (MTT) assay, Hoechst-33258 dye staining, flow cytometry (cell cycle), and Western blotting for caspase-3 and poly (ADP-ribose) polymerase (PARP). We found sophoraflavanone G induced the apoptosis of KB cells in a dose-dependent manner that was verified by DNA fragmentation, apoptotic bodies, the sub-G1 ratio, caspase-3 activity, and cleavage of PARP. These results suggest that sophoraflavanone G has potent anti-proliferative effects on human oral epidermoid carcinoma cells, with the induction of apoptosis.
Dan-Be Park,Jung-Il Cho,Hyemin Lim,Zamin Shaheed Siddiqui,Sung-Han Park,Mi-Jeong Jeong,Gang-Seob Lee,Soo-Chul Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Transgenic potatoes expressing glyceraldehyde-3-phosphate dehydrogenase (GPD), isolated from the oyster mushroom, Pleurotus sajor-caju, had increased tolerance to salt stress (Jeong et al. 2001). To examine the physiological mechanisms enhancing salt tolerance in GPD transgenic rice plants, the salt tolerance of five GPD transgenic rice lines (T1–T5) derived from Dongjin rice cultivar was tested in a fixed 150-mM saline environment in comparison to two known wild-type rice cultivars, Dongjin (salt sensitive) and Pokali (salt tolerant). Transgenic lines T2, T3, and T5 showed a substantial increase in biomass and relative water content compared to Dongjin. Stomatal conductance and osmotic potential were higher in the GPD transgenic lines and were similar to those in Pokali. The results are discussed based on the comparative physiological response of GPD transgenic lines with those of the salt-sensitive and salt-tolerant rice cultivars.