Valinomycin, produced by <i>Streptomyces</i> sp. S8, a key antifungal metabolite in large patch disease suppressiveness

Jeon, Chang?Wook,Kim, Da-Ran,Kwak, Youn-Sig Rapid Communications of Oxford Ltd in association 2019 World journal of microbiology biotechnology Vol.35 No.8

<P> Large patch disease, caused by <I>Rhizoctonia solani</I> AG2-2, is the most devastating disease in Zoysiagrass <I>(Zoysia japonica).</I> Current large patch disease control strategies rely primarily upon the use of chemical pesticides. <I>Streptomyces</I> sp. S8 is known to possess exceptional antagonistic properties that could potentially suppress the large patch pathogen found at turfgrass plantations. This study aims to demonstrate the feasibility of using the strain as a biological control mechanism. Sequencing of the S8 strain genome revealed a valinomycin biosynthesis gene cluster. This cluster is composed of the <I>vlm1</I> and <I>vlm2</I> genes, which are known to produce antifungal compounds. In order to verify this finding for the large patch pathogen, a valinomycin biosynthesis knockout mutant was created via the CRISPR/Cas9 system. The mutant lost antifungal activity against the large patch pathogen. Consequently, it is anticipated that eco-friendly microbial preparations derived from the S8 strain can be utilized to biologically control large patch disease. </P>

관상동맥질환자에서 경흉부 도플러 심초음파를 이용한 좌심실 확장기 기능의 평가 : 좌심실 확장기 장애 형태에 따른 분류

전희경,윤호중,유기동,박지원,전두수,정욱성,채장성,김재형,최규보,홍순조 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.1

의미 치매 1예 : 신경심리 및 뇌영상 검사의 연속적인 추적 관찰 소견 Serial Follow-up Study of Neuropsychological and Neuroimaging Findings

박경원,김욱주,전진민,강도영,김재우 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.1

Semantic dementia (SD) is a very rare, distinct form of dementia which is characterized by impairment of naming and comprehension, prosopagnosia, visual agnosia for objects and relative preservation of other aspects of cognition, such as episodic memory, nonverbal problem-solving and visuospatial functions. A 68-year-old man visited our clinic for progressive speech disturbance since 1997. His main speech problems were word finding difficulty and loss of word meaning for objects. Language assessment demonstrated profound semantic memory loss for words with preserved fluency, corresponding to anomic aphasia. There were marked impairments in verbal and visual memory functions and word fluency tasks, but no grammatical or phonologic errors in speech. Also were remarkable anosognosia and object visual agnosia with preserved visuospatial function and calculation in neuropsychological tests. Serial follow-up study of language test showed more deterioration in comprehension and repetition, consistent with Wernicke's aphasia. Initial brain MR imaging and brain perfusion SPECT showed prominent focal atrophy in the left temporal lobe, and marked hypoperfusion in the left temporal and both frontal areas. Follow-up brain MR imaging and perfusion SPECT revealed more atrophy in both temporal lobes and showed more extensive hypoperfusion areas compared to the previous study.

주의력결핍-과잉행동장애를 동반한 알코올 의존환자들의 특성 : 임상적/유전학적 자료분석

김붕년,손기현,전지현,곽욱환,유희정,조수철,박철수 大韓神經精神醫學會 2003 신경정신의학 Vol.42 No.3

Objectives : This study was conducted to estimate the comorbidity rate of ADHD in a group of patients with alcohol dependency and to find out the characteristics of alcoholic patients with ADHD using the diverse clinical and genetic variables. Methods : Eighty five patients with alcohol dependency were recruited from 4 mental hospitals in Kyoung-Nam and Kyoung-Ki province. For the evaluation of ADHD symptoms in both childhood and adulthood, the highly structured Diagnostic Interview Schedule for Children-IV (DISC-FV)-ADHD module was used. The various standardized scales and questionnaires were also applied to evaluate the comorbid conditions and psychopathological status. All the subjects' blood was collected and genetic study for the polymorphism of DRD2, TH, 5-HTTLPR, COMT, ALDH2 was performed. Results : 1) The comorbid rate of definite ADHD in alcoholic patients was 38% (28/85). 2) The frequency of unmarried status was significantly higher in ADHD group compared non-ADHD group. 3) The onset of pathologic drinking and auditory hallucination was significantly earlier in ADHD group than non-ADHD alcholic group. 4) In ADHD alcoholic group, antisocial behavior was more frequently reported, and the score of co-dependency scale, depression/anxiety, aggression and obsessive compulsive drinking scales were significantly higher compared to non-ADHD alcoholic group. 5) No signficant difference was found in the frequency of polymorphic alleles in COMT, DRD2, 5-HTTLPR, ALDH2, TH between ADHD and non-ADHD alcoholic group. Conclusion : In alcoholic patients, the rate of adult type ADHD was higher than expected rate in general population. The alcoholic patients with ADHD suffered from more sever degree of alcohol dependency and earlier alcohol related problems. ADHD-alcoholic group showed higher diverse comorbid psychopathology and lower marital status compared to non-ADHD-alcoholic group. No difference, however, was found in genetic data between two groups.

소아에서 간질발작의 임상양상과 뇌파검사의 관계

이승현(Seung Hyeon Lee),손동우(Dong Woo Son),은병욱(Byung Wook Eun),심소연(So Yeon Sim),최덕영(Deok Young Choi),선용한(Yong Han Sun),조강호(Kang Ho Cho0, 류 일(Eell Ryoo),전인상(In Sang Jeon0, 차 한(Hann Tchah) 대한소아신경학회 2009 대한소아신경학회지 Vol.17 No.2

목적:발작의 분류는 발작의 원인, 예후를 추정하거나 항경련제를 선택하는 데 있어 매우 중요하며 뇌파검사는 발작의 종류를 구분하고 적절한 치료를 하기 위해 시행하는 중요한 검사이다. 저자들은 소아에서 임상적 발작이 확실한 경우 간질발작의 임상양상과 뇌파검사 간의 일치 정도를 알아보고자 한다. 방법:2000년 1월 1일부터 2008년 9월 30일까지 1회 이상의 간질발작을 주소로 가천의대 길병원 소아청소년과에 방문한 환자 중 본원에서 처음으로 뇌파검사를 시행한 환자를 대상으로 하였다. 발작의 임상양상은 목격자와 본인의 설명을 기록한 의무기록지를 기준으로 하여 부분발작과 전신발작으로 분류하였고 뇌파검사는 발작간간질양방전을 기준으로 정상과 비정상으로 분류하였으며 각성과 수면상태 모두에서 시행하였다. 결과:총 461명의 환자의 뇌파검사 시의 평균 연령은 6.7세였고 남자는 247명, 여자는 214명이었다. 전체 환자에서 발작의 임상양상은 부분발작이 310명, 전신발작이 187명이었다. 각성뇌파검사에서 비정상은 158명으로 부분발작파가 118명, 전신발작파가 59명, 두 가지 발작파가 한 환자에서 나온 경우가 19명이었고, 수면뇌파검사에서는 비정상이 239명으로 부분발작파가 196명, 전신발작파가 77명, 두 가지 발작파가 한 환자에서 나온 경우가 34명이었으며 전체 환자에서는 비정상이 273명으로 부분발작파가 216명, 전신발작파가 97명, 두 가지 발작파가 한 환자에서 나온 경우가 40명이었다. 간질증후군은 전체 환자 중 90명에서 관찰되었다. 146명(41.5%)의 부분발작과 44명(23.4%)의 전신발작 및 79명(87.8 %)의 간질증후군이 뇌파소견과 일치하였다. 결론:영유아 및 소아에 있어 발작을 진단할 때에는 발작양상 분 아니라 정확한 뇌파소견을 세심하게 고려하여야 한다. Purpose:Electroencephalography(EEG) is an essential method carried out for classifying seizures and taking appropriate treatment. The aim of this study is to investigate the concordance between clinical findings of epileptic seizures and EEG in children. Methods:We enrolled 461 patients from those who visited Gil hospital from January 1, 2000 to September 30, 2008 with the chief complaint of epileptic seizure more than once and checked their first EEG at the same hospital. The clinical findings of seizure were based on the charts and interictal waking and sleep EEGs were done. Results:The mean age of the patients was 6.7 years old. 497 epileptic seizures occurred and its clinical finding included 310 of partial seizures and 187 of generalized seizures. In 315 waking EEG 158 were abnormal including 118 of partial seizures, 59 of generalized seizures and 19 of both seizures, in 431 sleep EEG 239 were abnormal including 196 of partial seizures, 77 of generalized seizures and 34 of both seizures, and in waking and/or sleep EEG 273 were abnormal including 216 of partial seizures, 97 of generalized seizures and 40 of both seizures. Epileptic syndromes were observed in 90 patients. 146(41.5%) of partial seizures, 44(23.4%) of generalized seizures, and 79(87.8%) of epileptic syndromes accorded with EEG. Conclusion:When diagnosing seizures in children, we must consider not only clinical findings but also accurate EEG findings.

과학 교과와 연계된 통합 교육의 국내 연구 동향 분석

이효녕,권영륜,김성년,손성호,한욱신,홍성구,박병열,전재돈 이화여자대학교 교과교육연구소 2014 교과교육학연구 Vol.18 No.2

This study analyzed trends in domestic research over the last twelve years before and after the 2009 curriculum revision with respect to integrated science education. The data was reviewed and collected from masters theses, doctoral dissertations, and research articles published from 2001 to 2012. An analyzing framework was developed through literature reviews and content analysis of the previous studies. A total of 329 studies were selected and analyzed by year, type, content, and object of research. In addition, the method of organization of the subject, the elements of integration, and the method of integration among different disciplines were additionally analyzed to discover the trends in integrated teaching-learning programs. The results indicated that the number of domestic studies related to integrated science education increased from 2001 to 2012. The development and application of teaching-learning programs as a form of research, teaching and learning as a research topic, and elementary school students as research objects were conducted more often than other studies. According to an analysis of teaching-learning programs, the subject matter integrated most often with science was fine art, and a theme-based integration approach was applied to the majority of the forms of integrated education. It is therefore recommended that development of teaching and learning materials and teacher training programs in integrated science education will be needed to foster students’ integrated scientific literacy. 이 연구의 목적은 2009 개정 과학과 교육과정이 도입되기 전후 12년 동안 과학교과와 연계된 통합교육의 국내연구는 어떻게 이루어져 왔는지 알아보는 것이다. 이 연구문제를 위해 2001년부터 2012년까지 학술지에 게재된 논문과 학위 논문 329편을 대상으로 그 동향을 분석하였다. 과학교육 연구 측면에서의 연구동향을 알아보기 위해 이론적 배경을 통해 분석틀을 제작 하고 제작한 분석틀에 따라 연도별 연구 형태,연구 방법, 연구 내용, 연구 대상을 분석하였다. 또 분석 대상 논문 중 교수 · 학습 프로그램을 개발한 논문을 개발한 분석틀에 따라 어떤 교과가 과학교과와 통합이 이루어졌는지, 교과의 조직방식, 통합의 조직요소, 학문이 연결되는 방식이 어떠한지를 분석하였다. 연구의 결과 과학교과와 연계된 통합교육의 연구논문이 증가하였고, 연구 유형으로는 교수 · 학습 프로그램의 개발과 적용, 연구 주제는 교수 · 학습, 연구대상으로는 초등학생이 많았다. 지난 12년 동안 개발된 교수 · 학습 프로그램을 분석한 결과, 과학교과와통합을 가장 많이 한 교과는 미술이었고, 통합의 유형은 기여적 통합, 주제 중심 통합, 간학문적 통합이 가장 많았다. 본 연구의 결과를 바탕으로 학생들의 융합적인 소양의 배양을 위해 통합 과학 교육 교수학습 자료의 개발과 교사 연수프로그램의 개발 등을 제언하였다.

심인성 색전 요인이 없는 뇌경색 환자에서 경식도 심초음파와 Acoustic Quantification을 이용한 대동맥 탄성의 평가

이종민,윤호중,임효영,박지원,김휘열,전희경,문건웅,정욱성,채장성,김재형,최규보,홍순조 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

심첨부 비후 심근증에서 심근 내 미세 관상동맥의 혈류 예비력 평가 : 경흉부 도플러 심초음파를 이용한 연구 Study Using Transthoracic Doppler Echocardiography

윤호중,박철수,신재원,임상현,조은주,정해억,전희경,오용석,정욱성,김재형,최규보,홍순조 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.3

A DIODE DESIGN STUDY OF THE VIRTUAL CATHODE OSCILLATOR WITH RING-TYPE REFLECTOR

Wook Jeon,Kew Yong Sung,Eun Ha Choi,Jin Gi Kim,Yoon Jung 한국물리학회 2004 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.44 No.52

A numerical simulation study of high-power microwave generation from the vircator (virtual cathode oscillator) is carried out for a coaxial diode structure by using a 3-dimensional particle-incell (PIC) code called MAGIC. The coaxial vircator has a cylindrical cathode, a cylindrically meshed anode, and a re ector in order to enlarge the active interaction region in the virtual cathode space. The simulation results show that the output microwave frequency has a narrow bandwidth and that the output microwave power sensitively depends upon the position and the width of ring-type re ector. The maximum output microwave power is obtained by adopting a ring-type re ector 10 mm in width and 40 mm in distance from the intense relativistic electron beam. The output microwave mode without the ring-type re ector is a mixture of TM and TE modes. However, the TM01 mode with its resonance frequency of 2.2 GHz is dominant for the ring-type re ector.

Effect of tungsten nanoparticle on the microstructure and mechanical properties of solder and strengthening theory of nano-composite solder

Wook Sang Jeon(전욱상),Rajendran Sri Harini,Bum Gyu Baek(백범규),Song Hee Yim(임송희),Jae Pil Jung(정재필) 대한용접·접합학회 2019 대한용접학회 특별강연 및 학술발표대회 개요집 Vol.2019 No.11