Alterations in DNA methylation patterns in regenerated Chinese cabbage (Brassica rapa ssp. pekinensis) plants derived from tissue culture

Hyun‑Min Lee,Jee‑Soo Park1,Yun‑Hee Shin,박영두 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.4

Plant tissue culture is an essential tool in biotechnology. However, tissue-cultured plants often exhibit variations that are either genetic or epigenetic in origin, termed somaclonal variations. Among these variations, DNA methylation is an important heritable epigenetic modification that plays a role in a wide variety of biological processes, including gene expression. In this study, we performed bisulfite sequencing of regenerated Chinese cabbage (Brassica rapa ssp. pekinensis) lines to identify DNA alterations induced by tissue culture. Sequencing data from each regenerated line were compared with reference genome sequences, and common differentially methylated regions (DMRs) were detected in the regenerants. To determine changes in expression levels of DMR-containing genes, we performed quantitative reverse transcription-polymerase chain reaction (qRT-PCR) of the target genes and PCR amplification with bisulfite-converted DNA. We identified DMRs between a non-regenerant line and regenerant lines and selected 10 DMR-associated genes that presented annotation information in Arabidopsis or Brassica rapa. Their expression levels were verified by qRT-PCR to determine the relation between methylation state and gene expression. We observed that genes positioned in DMRs significantly correlated with differential gene expression. We also observed similar methylation patterns in the selected DMRs by PCR-based methylation analysis. The results of this study are a valuable resource for the epigenetic analysis of regenerated lines, especially for Chinese cabbage.

A Study on Joke Misinterpretation in L2 Settings: Viewed from the Benign-Violation Theory

Hyun-jee Kang,오명기(Myung-ki Oh) 현대문법학회 2018 현대문법연구 Vol.99 No.-

Hyun-jee Kang and Myungki Oh. 2018. A Study on Joke Misinterpretation in L2 Settings: Viewed from The Benign-Violation Theory. Studies in Modern Grammar 99, 161-176. This paper examines cases in which Korean college students might misinterpret superiority, incongruity, and sarcastic English jokes. In parallel the writer tries to bring to light possible background reasons for their misinterpretation from the perspective of the Benign-Violation Theory’ concepts of norm violation, commitment, and psychological distance. Arguably Korean college students are likely to misinterpret English humor codes influenced by their ingrained belief system of strict moral standard as well as insufficient exposure to particular genres of English jokes.

명청교체기 인평대군 시문학 연구

이현지(Lee, Hyun-jee) 한국어문학회 2016 語文學 Vol.0 No.132

Royal families were supposed to live idle lives, but the life of Prince Inpyeong was an exceptional case. Prince Inpyeong"s role has been evaluated as the absolute peace in the King Hyojong era. To solve sensitive diplomatic issues between Choson-Qing smoothly, Inpyeong took the critical role of an envoy. He took on almost all the difficult tasks in diplomacy. All his diplomatic journeys and achievements had the source in the close loving relationship with the king. The prince played a crucial role to cause the fall of Ming Dynasty and watched Qing Dynasty during the journeys. He provided information of the internal affairs of Qing Dynasty to King Hyojong. It was a significant source to make Hyojmg’s Plan to Conquer the North possible. His poetry was mainly to communicate with King Hyojong. The poems seek after a hero, enjoy the feeling of freedom elegantly, show sentiment of friendship and loyalty for his king and brother, Hyojong.

재발성 복부 통증을 호소한 환자에서 복벽에 발생한 자궁내막증 1예

홍현진,박성한,이준식,배용목,김지연,안광순,박선자 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.1

Endometriosis is the abnormal growth of endometrial tissue outside the uterine cavity. Endometriosis in the abdominal scar following cesarean section is very rare condition among the extrapelvic endometriosis. The frequency of abdominal wall endometriosis secondary to cesarean section is from 0.03% to 0.8% in some reports. It is difficult to diagnosed in spite of the typical symptoms. The typical symptom is aching swelling in the area of the surgical scar, which is influenced by the phases of menstruation. The definitive diagnosis is established by pathologic analysis. Surgical excision remains the treatment of choice. The majority of patients presented from 1 to 2 years after the precipitating operation. We experienced an unusual case of abdominal wall endometriosis presented over 10 years after cesarean section, who was admitted to our hospital complaining of recurrent left lower abdominal pain. So, we report this case with a brief review of the concerned literatures.

뇌전이 융모암 : 증례 보고 Case Report

현용인,강재규,도종웅,김기욱,김상진 최신의학사 1990 最新醫學 Vol.33 No.12

만성정신분열증의 정량화 뇌파검사 소견

지재현,설현욱,조경형 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.1

본 연구는 만성 정신분열증 환자 30명과 정상대조군 21명의 뇌파 중 델타파와 쎄타파를 분석해 봄으로써 만성 정신분열증 환자의 국소뇌파 소견과 유병기간, 약물투여량 및 투여기간, 양성증상과 음성증상에 따른 뇌파소견을 보고자하였다. 연구결과는 다음과 같다. 첫째, 만성 정신분열증 환자와 정상대조군간에 델타파와 쎄타파의 국소뇌파량을 t 검증한 결과 델타파에는 유의한 차이가 없으나, 쎄타파는 전 부위에서 유의한 차이를 보였다. 둘째, 만성정신분열증 환자의 델타파 및 쎄타파는 유병기간에 따른 유의한 차이가 없고, 투약기간이 길어질수록 F7, T3, C3, C4의 델타가 감소하는 경향을 보이고, 항정신병약물 투여량이 많을수록 P3, P4, C4, T5, T6, O1, O2의 쎄타가 증가하는 경향을 보였다. 셋째, 양성증상의 유무에 따라 Fp2의 델타에 유의한 차이를 보이고, 음성증상의 유무에 따라서는 Fp2, T3, C3, P3, O1의 델타에 유의한 차이를 보였다. 넷째, 판별분석을 시행한 결과 qEEG의 델타파와 쎄타파의 국소 뇌파량만으로 만성정신분열증 환자 30명중 29명을 (96.7%) 만성 정신분열증으로 판별할 수 있었다. 이상에서 만성정신분열병 환자의 서파가 정상대조군과는 유의한 차이를 보이며 유병기간에 따른 차이는 없으나 투약기간과 투여량에 따른 차이를 보이고 음성 증상을 가진 환자에서 좌측 대뇌반구의 기능저하를 추측케한다. Electroencephalographic recordings in 30 medicated patients fulfilling the DSM-Ⅲ-R criteria for chronic schizophrenia and 21 normal healthy controls was studied, using computerized electroencephalography. The delta activity and theta activity were compared between the patients and the normal healthy controls, then the differences in delta activity and theta activity of the patients were analyzed according to duration of illness, duration of medication, dosage of medication, and the presence of positive and negative symptoms. In the comparison study of chronic schizophrenic patients and the normal healthy controls, the theta activity showed a significant difference in all regions, and a significant difference in abnormal electroencephalographic activity appearance rate. In the analysis of the delta and theta activities of the chronic schizophrenic patients, duration of illness had no significant influence, the delta activity in F7, T4, C3, and C4 regions showed a tendency to decrease as duration of medication increased, the theta activity in P3, P4, C4, T5, T6, O1, O2 regions showed a tendency to increased with the increment of dosage of neuroleptics. The delta activity in Fp2, T3, C3, P3, O1 regions showed a significant difference between those patients with and without negative symptoms. In summary, the theta activity of the chronic schizophrenic patients showed a significant difference in comparison with that of the normal controls. In chronic schizophrenic patients, no difference in electroencephalograph was apparent regarding the duration of illness, but significant differences were shown when analyzed regarding the duration and dosage of medication. According to the results of this study, dysfunction of left cerebral hemisphere could be presumed to be present in patients with negative symptoms.

목둘레와 심혈관계 위험인자와의 연관성

강지현,유병연 대한비만학회 2003 The Korean journal of obesity Vol.12 No.2

연구배경: 최근 단순 비만 지표로서의 목둘레의 유용성에 대한 연구들이 발표되고 있으나 국내에서는 아직 목둘레와 심혈관계 위험인자와의 관련성에 대한 연구가 없었다. 이에 한국 성인 남녀에서 목둘레와 심혈관계 위험인자들과의 연관성을 알아보고 체질량 지수. 허리둘레, 체지방률 등과 비만 지표로서의 목둘레의 유용성을 비교해보기 위하여 본 연구를 시행하였다. 방법: 일개 대학 병원 종합 검진 센터에 내원한 남녀 292명을 대상으로 목둘fp와 심혈관계 위험인자와의 상관관차를 알아보고 목둘fp를 4분위 (quartiles)로 나누어 가장 낮은 목둘레 4분위군과 가장 높은 목둘레 4분위군 간 심혈관계 질환들의 교차비 (odds ratio)를 로지스틱 회귀분석을 통하여 구해 보았다. 체질량 지수. 허리둘레, 체지방률 등과 비교하여 목둘레가 독립적으로 심혈관계 위험인자들을 설명할 수 있는 변수인지를 단계별 다중 회기 분석 (stepwise multiple regression analysis)을 통하여 비교하였다. 결과: 목둘레는 남자에서 수축기 혈압 및 요산, 여자에서 이완기 혈압. 총콜레스데롤 및 저밀도 콜레스테롤을 제외한 심혈관계 위험인자들과 유의한 상관관차를 보였으며, 가장 높은 목둘레 사분위군에서 가장 낮은 목둘레 사분위군에 비해 HOMA score는 남자 29.2배, 여자 5.1배 증가하였곤, hsCRP는 남자2.0배, 여자 6.6배 증가하는 것으로 나타났다 단계별 다중 회기 분석에서 목둘레는 다른 비만 지표들에 독립적으로 남자에서는 이완기 혈압. 혈당, 총 콜레스테롤, 저밀도 콜레스테롤, 중성지방, 공복시 인슐린, HOMA score, 여자에서는 공복시 혈당, 고밀도 콜레스테롤, 공복시 인슐린 HOMA score의 변화에 기여하는 것으로 나타났다. 결론: 목둘레는 인슐린 저항성 및 hsCRP의 상승과 관련된 심혈관계 위험인자들과 연관되어 있으며 심혈관계 질환의 위험이 증가되어 있는 비만 환자의 선별 검사로 유용하게 쓰일 수 있을 것으로 생각된다. Backgrounds: The aim of the present study was to test the association of neck circumfernce with the cardiovascular risk factors. Methods: We examined 292 persons, who visited a health examination center. The correlation between neck circumference and other cardiovascular risk factors was tested, and the odds ratio was calculated for various cardiovascular risk factors in the highest quartile of neck circumference compared to lowest. We used stepwise multiple regression to find out whether the neck circumference could be the independent variable to other obesity indices for cardiovascular risk factors. Results: Neck circumference was significantly correlated with the cardiovascular risk factors except diastolic blood pressure, uric acid in men and systolic blood pressure, total and LDL cholesterol in women. This odds ratio for HOMA score in the highest quartile of neck circumference compared to lowest was 29.2 in men, 5.1 in women, and for hsCRP the odds ratios was 2.0 in men, 6.6 in women. In stepwise multiple regression, neck circumference accounted for diastolic blood pressure, total and LDL cholesterol, triglyceride, insulin, HOMA score in men, glucose, HDL cholesterol insulin, HOMA score in women independently to other obesity indices. Conclusion: Neck circumference is associated with the cardiovascular risk factors related to insulin resistance and hsCRP. The measurement of neck circumference could be useful in clinical screening for obese person at an enhanced risk for cardiovascular diseases.

Genetic Algorithm을 이용한 철근콘크리트보의 최적설계

안지현,이차돈 中央大學校 建設環境硏究所 1999 環境科學硏究 Vol.9 No.2

Simple Genetic Algorithm (SGA) is refined to take into account for the optimization of reinforced concrete beams under positive and negative moments. GA has beneficial aspects compared to conventional numerical or analytical optimization techniques in that ; it can handle discrete variables, does not need continuity or derivatives of a function, has more probabilities of convergence to global minimum; and is relatively easy to include the equations in structural design code. Considered in optimization are : discrete set of widths, depths and number of rebars both in positive moment and in negative moment region for beam members. In addition to the strength constraints, requirements for reinforcement arrangement including minimum cover thickness and necessary spacings between rebars in a beam section are considered. Within a reasonable and finite number of searching, the developed algorithm was able to yield optimum solutions for reinforced concrete beams.

컨벤션 및 관광객 유치 기구(CVB)의 웹사이트 분석 : focused on the Internet Marketing Strategy

박현지 문화관광연구학회 1999 문화관광연구 Vol.1 No.1

ABSTRACTThe appearance of Internet brought about enormous effects and changes in the tourism industry. The settlement of municipalism also means economically tremendous impact of tourism.CVB(Convention and Visitors Bureau) websites simultaneously reflect these changes. Therefore, CVB is a tool for tourism marketing in municipalism and CVB websites are utilized for Internet marketing tool.In this paper, Internet marketing, Internet marketing mix and the concepts of CVB are studied.As empirical analyses, the analysis of components in CVB homepage, the analysis of contents in CVB website based on Internet marketing mix and the analysis of users' responses on CVB websites are investigated.

고정희論 : 불의 魂과 물의 詩

송지현 목포대학교 여성문제연구소 1996 여성연구 Vol.4 No.-