Study of decreased melanin production through p53 by heme oxygenase-1 inhibitor in normal human melanocytes

( Hee Sun Lim ),( Sun A Jin ),( Jee Bum Lee ),( Seong Jin Kim ),( Seung Chul Lee ),( Young Ho Won ),( Sook Jung Yoon ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Heme oxygenase (HO)-1 is induced by oxidative stress and plays important roles in anti-apoptosis and the rapid growth of several solid tumors. p53 has a central role in skin pigmentation and may impact onmelanoma at all stages. However, there has been little study of HO-1 in relation with p53 on melanin production Objectives: To know the effect of HO-1 on melanogenesis through p53 in normal human melanocytes Methods: Human melanocytes (hM) were primarily cultured from foreskin. After incubation, cells were rinsed twice with PBS then transfection with p53 siRNA Results: Melanin content was detected with ELISA and Western blot analysis and RT-PCR of tyrosinase, MITF were performed after ZnPP treatment and p53 transfection. After ZnPP treatment, melanin content was decreased, and tyrosinase and MITF protein levels were decreased. Tyrosinase and MITF mRNA levels were also decreased. However, melanin content and tyrosinase and MITF protein levels were increased after CoPP treatment. After p53 transfection, HO-1 expression was decreased when HO-1 stimulator was treated. In addition, melanin content was decreased, and tyrosinase expression was decreased in Western blot analysis Conclusion: These results suggest that melanogenesis is inhibited by ZnPP by decreased melanin production, tyrosinase and MITF protein and mRNA levels. Furthermore, those inhibitory effects of ZnPP may be dependent on p53 in normal human melanocytes. Therefore, HO-1 may play an important role in melanogensis

A Case Study on the Fire of Factory Buildings in Korea

Jee,Nam-Yong,Lee,Kyung-Ku,Suh,Chee-Ho 한국화재소방학회 1997 한국화재소방학회 학술대회 논문집 Vol.1997 No.-

According to the fire statistics of the Ministry of Home Affairs, the fire ratio in factories, work places and warehouses shows 21.5% among total fire of buildings except the fire on cars or other fires. Especially, the buildings like factories and warehouses, which have a lot of ignitable sources and thus always have a strong possibility of fire, are needed to have well planned strategy in terms of life safety and property protection. In this respect, the present paper analyses the existing fire cases in factories, finds out the fire properties of factory buildings, and evaluates the codes concerning fire resistance and fire severity.

부신피질 기능저하증에 대한 치료 1례

김지은,박형진,송근호 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2011 動物醫科學硏究誌 Vol.18 No.1

A 11-year-old castrated mongrel dog with the hypoadrenocorticism was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. Hypoadrenocorticism was diagnosed by the clinical signs, the physical examination, laboratory examinations(complete blood count, serum chemistry), ACTH stimulation test and radiography. Clinical signs were improved after fludrocortisone treatment on the basis of ACTH stiluation test.

문화요인이 국가간 회계정보 공시수준에 미치는 영향

崔炳皓,張志仁 韓日經商學會 1999 韓日經商論集 Vol.17 No.-

The culture has been affected by environmental factors, has grown and has progressed. Because of various international cultural differences, the social and cultural values affect accounting values, and finally a channel and proper degree of accounting practices. The purpose of this study is to examine empirically the determinants of the level of accounting disclosure in international context. More specipically the study analyzes the effects of cultural and economic factors on the level of accounting information disclosures in 38 countries based on Gray(1983)'s accounting value model using accounting disclosures indices published by CIFAR. For an empirical analysis, the study uses Hofstede,s five indices of cultural values and two economic variables as independent variables, and the disclosure indices of CIFAR as dependent variables. Five cultural variables are individualism, power distance, uncertainty avoidance, masculinity, and long-term orientation, and two economic variables being the size of the capital market and the degree of overseas exposure of the national economy. For the univariate analysis, the study uses the t-Test and the Mann-Whitney U Test for investigating the effects of each independent variables. From the results of this analysis, statistically significant factors are individualism, power distance, uncertainty avoidance among cultual variables, although masculinity and long-term orientation are not. Economic variables are generally significant. For the multivariate analysis, multiple regression are employed. The result of multiple regression using cultural variables corresponds with that of univariate analysis in other cultural variables except power distance. The result of multiple regression using economic variables doesn't corresponds with that of univariate analysis in model. The size of capital market and the degree of overseas exposure of the national economy(1) appear to be contrary to the expected relationship in Model. It is estimated because of the correlation among independent variables that the result of univariate analysis doesn't correspond with that of multivariate analysis.

표피 줄기세포

최지호 大韓皮膚硏究學會 2003 大韓皮膚硏究學會誌 Vol.10 No.4

벼 褐色葉枯病에 關한 硏究 : 벼 品種間抵抗性에 對하여 Rice Vrietal Resistance to Rice leaf Scald

金文鎬,李準琸,孫再根 慶北大學校 1978 論文集 Vol.25-26 No.-

This experiment was conducted to obtain basic information on the breeding for the disease resistnat rice varieties by clarifying the degree of varietal resistance to Rhynchosporium oryzae which was known as a new disease of the rice variety "Tongil" in Korea. The results obtained are as follows; 1. The varietal difference of resistance to this fungus showed some similarity in the spraying inoculation at the seedling stage, but the ratio of lesion enlargement in the soaking inoculation at the heading tsage showed remarkable difference. 2. Indicatype and Indica x Japonica type varieties were relatively more susceptible to this fungus than Japonica type varieties. 3. Among the Japonica type varieties tested, upland rice varieties were more susceptible than paddy rice varieties, and Korean developed varieties such as Jaekeun, Nongbaek, Palkeum, Pungkwang, etc., were more resistant than Korean native and introduced varieties. The most susceptible varieties among the tested varieties were Suweon 264, 230, 265, 262, Milyang 29, Iri 326, etc., which were Indica Japonica crosses.

스포츠 소비자의 가치관과 스포츠 선호종목의 관계

장호중,김지은,김정묵,정철규,박순문 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.20 No.1

The purpose of this study is to examine the relationship between The Relationship between and Sports Preference Event and Value of Sports consumers. To achieve this purpose, the subjects were selected by cluster random sampling of adults in sports facilities, in Seoul, in 2001 at present. There were 414 male, 360 female used for the analysis. The total was 774. Data were collected by employing a questionnaire. The variables of this analysis were conducted value, sports items, demographic characteristics. The survey questionnaires were used to collect the data. The questionnaire for the formalization factor of a Value of Sports consumers by Kim(1996). The collected data analyzed statistic program SPSS Windows 10.0 Version. The statistical analysis are descriptive statistics analysis, factor analysis, one-way ANOVA, crosstabs, discriminant analysis. Through this process, the study has reached the following conclusions. First, value of sport consumers, sports event, are partly different by demographic characteristics. Second, Value of Sports consumers are influence of Sports Preference Event.

生物濾過를 利用한 侵出水 處理에 관한 硏究

宋在峻,李榮好 木浦大學校 工業技術硏究所 1995 工業技術硏究誌 Vol.5 No.-

Leachates from solid waste landfill in Kwangju City was collected, and its characteristics were analyzed. The leachate treatment removal characteristics of bio-filter filled with Netring filter media were experimentally examined with constant temperature, pH value and variable BOD loading and recirculation ratio. The results obtained are as follows: pH, TCOD, TBOD5, TKN, T-P's average concentrations were 6.5, 24,320mg/ℓ, 14,914mg/ℓ, 722.4mg/ℓ, 2.94mg/ℓ respectively. The ratio of TBOD/TCOD was 0.61 most of the organics is biodegradable by microorganisms, thus judged to require biological treatment. As a result of treatment with submerged biofilter maintaining F/M ratio of leachate at 0.1~0.4kg BOD/ kg MLVSS. day, the efficiency of BOD5 removal was 69.9~96.1% in raw leachate treatment process, and anaerobic digestive leachate process obtained BOD5 removal efficiency 51.4~90.7%. The BOD5 removal efficiency increases with the recirculation ratio, but the effenciency of increase becomes smaller as the recirculation ratio increases over 50.

고강도 콘크리트 전단강도 추정식의 제안

강원호,성기태,김일봉,방지환 동아대학교 건설기술연구소 1997 硏究報告 Vol.21 No.2

Recently, high strength concrete members are used increasingly, but most code specifications are based on the previous studies of concrete members whose strength are lower than 400 kg/㎠. In this study, we checked validity of the used shear design specifications of ACI and EC2 for high strength concrete members. Further, we propose a shear strength evaluation equation from the analysis of test results of 433 members, which include high strength concrete. According to the comparison results, we can see that shear strength evaluation of ACI and EC2 has large deviation for high strength concrete members, and Zsutty's equation has a problem of nonuniform deviation. Our proposal, which comes from regression analysis of test results, can give tentative help for the practical engineer, as it has less deviation and lies in safe side.

학교급식과 비급식이 아동의 체격ㆍ체력에 미치는 영향

박지훈,백영호 釜山大學校 附設 體育科學硏究所 1995 體育科學硏究所 論文集 Vol.11 No.-

The purpose of this study was to investigate the effect of school-feeding & non school-feeding on physique and physical fitness in children. The subjects of this study were on 6th grade(11years, N=9,089 M:4599, F:4490) who took physique test, 1,277(M:656, F:621) children took physical fitness test in Pusan municipal elementary school. From the result and discussions of this study, the conclusions are as follows: 1. school-feeding A children was better than school-feeding B children and non school-feeding children in the physique(p<.001). 2. In physical fitness test, school-feeding children were superior in 100m dash and standing board jump with male and female(p<.001), non school-feeding children were superior in distance race(600m), throw(male), arm-pulls(female), sit-ups(male).