문제중심학습(Problem Based Learning; PBL) 패키지 개발 : 지각·조정장애상황을 중심으로

김애리,김영경,송영선,신경림,안혜정,이지순,조계화 성인간호학회 2001 성인간호학회지 Vol.13 No.3

The Purpose of this study is to present an actual example for procedures for developing a PBL package based on philosophical backgrounds derived from Problem-based learning. To perform a systemic study on the operations of an intergrated curricula under multi-disciplines, a research team made up of several professors with different academic backgrounds was formed. Among the four situations for the patients with perception-adjustment disorder, especially a procedure for the development of PBL package which can be used in the emergency room situation has been proposed. The little(2000)'s PBL package model has been applied for this study. Tha package includes course objectives, learning objectives, concept map, situation scenario. tutor guide, and evaluation method. It is believed that learning objectives achievement procedures designed as a part of a problem-based learning package development procedures for the nursing of patients with perception-adjustment can be achieved at the same level as the learning objectives for the science of nursing founded by the Korean Nurses Association.

Human Papillomavirus Testing with Hybrid Capture Ⅱ and DNA Chip

이덕철,--,--,-- 대한의생명과학회 2005 Biomedical Science Letters Vol.11 No.1

The detection of high-risk human papilloma virus (HPV) allows us to predict the presence and future development of cervical intraepithelial lesion. In this study, we compared Hybrid Capture II and DNA chip methods for detection of HPV in cervical swab samples. And we evaluated the clinical efficacy and diagnostic performance of HPV DNA chip and Hybrid Capture Ⅱ for detecting HPV in cervical neoplastic lesions. Seventy four patients were classified into three groups according to their histologic diagnosis: Group Ⅰ (nonspecific chronic cervicitis), Group Ⅱ (low-grade squamous intraepithelial lesion (SIL); koilocytosis, and mild dysplasia), and Group Ⅲ (high-grade SIL;, moderate, severe dysplasia and in situ carcinoma). Cytologic diagnosis were based on the Bethesda System. Hybrid Capture Ⅱ and DNA chip methods were performed to detect HPV. In 41 of the 74 cervical samples (55.4%), HPV DNAs were detected by Hybrid Capture Ⅱ. In Group Ⅲ, HPV-positive cases were detected in 15 (20.3%) of 74 patients by Hybrid Capture II. 25 patients with ASCUS cytology were histopathologically examined: 9 cases (36%) were Group Ⅱ. In 18 patients with low-grade SIL cytology, 13 cases (72.2%) were Group Ⅱ and 3 cases (16.7%) were Group Ⅲ. 12 cases (92.3%) were Group Ⅲ of 13 patients with high-grade SIL cytology. The sensitivity of each test was 82% in Hybrid Capture Ⅱ and 53.9% in DNA chip test. And the specificity was 74.3%, 85.7% in Hybrid Capture Ⅱ and DNA chip. In conclusion, Hybrid Capture Ⅱ test is more sensitive than DNA chip in detecting women with cervical neoplastic lesions. Especially, in diagnosing of ASCUS, Hybrid Capture Ⅱ test is more sensitive. Therefore, Hybrid Capture Ⅱ test for cancer-associated HPV DNA is a viable option in the management of women with ASCUS.

호르텐스극구흡충에서 Alkaline Phosphatase 및 Acid Phosphatase의 특성

강성구,양용석,박주연,임지애,김인식 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY 1999 Journal of biomedical laboratory sciences Vol.5 No.1

호르텐스극구흡충 성충 (adult worm)을 재료로 하여 acid phosphatase (Acp)와 alkaline phosphatase (Alp)의 조직 세포에 대한 분포 및 동위효소의 특성을 규명하기 위하여 Gomori lead nitrate 및 Gomori calcium 방법 그리고 전기영동법 등을 시행하였다. 조직 내의 Acp 및 Alp의 분포는 충체의 소장 (intestine)과 난황선 (vitellaria) 그리고 인두 (pharynx)에서 강한 반응을 나타냈다. 동위효소 유형은 Acp에서 179.5 및 209.4 kDa 두 개의 분획이 분리되었고, Alp은 145.9, 207.5 및 220.8 kDa의 세 개의 분획이 분리되었다. 열에 대한 안정도는 Alp가 90℃에서 12초 경과 후에 완전히 불활성화 되었으며, Alp의 최적 온도 및 pH는 40℃와 pH 9이었고 Acp의 최적 pH는 5였다. 그리고 Acp 및 Alp의 최대활성도 (unit)는 Acp가 71 범위였고, Alp는 189의 범위에 있었다. 이상에서와 같이 호르텐스극구흡충은 phosphatase가 소화관과 난황선에서 주로 분포하고 있었으며 숙주 체내에서 소화관을 중심으로 대사가 활발하게 이루어지고 있음을 알 수 있었다. This study was aimed to investigate the enzyme-histochemical localization and characteristics of alkaline and acid phosphatase extracted from adult of Echinostoma hortense. Using the Gomori calcium stain and the Gomori lead nitrate satin method, we found that the alkaline and acid phosphatases were localized mostly in the intestine, vitellaria and pharynx of Echinostoma hortense. The three isozymes of alkaline phosphatase and two isozymes of acid phosphatase were separated from Echinostoma hortense by electrophoresis. The isozymes of alkaline phosphatase were 145.9, 207.5, 220.8 kDa and the isozymes of acid phosphatase were 175.9 and 209.4 kDa. The activity of alkaline phosphatase was denatured completely after heating at 90℃ for 12 seconds. The optimum pH and temperature for activity of alkaline phosphatase were about pH 9 and 40℃, while the optimum pH for activity of acid phosphatase was about pH 5. The maximum activity of alkaline phosphatase was at 189 unit, but maximum activity of acid phosphatase was at 71 unit. As the result from above, we observed that alkaline and acid phosphatases funtion mainly in the alimentary tract and vitellaria. Echinostoma hortense performs the parasitism in the intestine of host by using proper isozyme of phosphatase.

Kinetics of Goblet Cells and Mast Cells in the Intestine of C3H/HeN and BALB/c Mice Infected with Echinostoma hortense

Jee-Aee Im,Insik Kim,Yoon-Kyung Jo,Kyu-Jae Lee,Yong-Suk Ryang 대한의생명과학회 2004 Biomedical Science Letters Vol.10 No.2

Mast cells and goblet cells have the ability to protect against parasites by increasing mucus production that traps and excludes worms and prevents their intimate contact with the gut mucosa in the host. In this study, we investigated the function of mast cells and goblet cells for the rejection of Echinostoma hortense (E. hortense). In addition, we used both C3H/HeN and BALB/c mice in order to examine whether mast cells and goblet cells function differentially according to the strains of mice. After an oral infection with 30 E. hortense metacercariae, the number of mucosal mast cells and goblet cells, as well as worm recovery rate, were observed in experimentally infected mice between 1 week and 8 weeks post-infection (PI). Worm recovery rates in C3H/HeN and BALB/c mice were 65.7% and 23%, respectively, in week 1 P.I., indicating that worm expulsion in C3H/HeN mice was higher than in BALB/c mice. Our results demonstrate that the period (week 3 P.I.) in which worm recovery falls rapidly is the same period that the number of goblet cells and mast cells reaches a peak. These results indicate that worm recovery significantly correlates with the growth rate of goblet cells and mast cells (P=0.0482). However, worm expulsion is not associated with goblet cells or mast cells in BALB/c mice.

Sarcopenia and Age-Related Changes in Body Composition in Korean Older Persons

Jee-Aee Im,Sang-Hwan Kim,Hee-jung Lim,Chang-hee Kim,Sang-Hoon Suh 대한의생명과학회 2008 Biomedical Science Letters Vol.14 No.1

We examined the prevalence of sarcopenia and age-related changes in body composition in Korean older persons. Community dwelling 77 men and 65 women (60~88 yr) were recruited for this study. Fat-free mass and the percent body fat were determined using bioelectrical impedance analysis. Isometric grip strength was measurement using grip strength dynamometer. Serum levels of fasting glucose, total cholesterol, HDL-cholesterol, and triglyceride were assayed. The prevalence of sarcopenia was found to increase with aging (men in their sixties 82.6%, seventies 96.6% and eighties 100%, and women in their sixties 47.4%, seventies 63%). In both gender groups, handgrip strength was inversely correlated with age and positively correlated with height, lean mass, and fat free mass. Better handgrip strength was related with higher weight in the men and with lower heart rate in the women. Fat free mass, age, and gender were found to be independent factors significantly associated with handgrip strength in the multivariate analysis. In summary, results of the present study suggest that handgrip strength is well associated with age, gender, and fat free mass and the prevalence of sarcopenia is increased with age and is exceeded by 40% among Korean persons older than 60 years.

Distribution of human Papillomavirus genotype and multiple infection by DNA Chip in Korean women

( Jee Aee Im ),( Ho Sang Lee ),( Ssang Sun Lee ),( Jin Kyung Kim ),( Sang Young Kim ),( Sang Hee Go ),( Dong Yul Seo ),( Kyung Ah Lee ),( Ja Young Jeon ),( Chang Hong Min ) 대한임상병리사협회 2005 조직세포검사학회 발표자료집 Vol.2005 No.-

BACKGROUND: We have investigated the prevalence of HPV DNA in cervical samples collected from 2,543 Korean women and examined the use of a new-human papillomavirus (HPV) detection method, the HPV oligonucleotide microarray system. METHODS: Nine hundred four patients were involved and classified into three groups according to their histopathologic diagnoses: Group I (nonspecific chronic cervicitis; n = 61), Group II (low-grade squamous intraepithelial lesion (SIL); koilocytosis, and mild dysplasia; n = 39), and Group III (high-grade SIL; moderate, severe dysplasia and in situ carcinoma; n = 40). Cytological diagnoses were based on the Bethesda System. The HPV oligonucleotide microarray detected 15 types of high-risk HPV (HPV-16/-18/-31/-33/-35/-39/-45/-51/-52/-56/-58/-59/-66/-68/-69) and 7 types of low-risk HPV (HPV-6/-11/-34/-40/-42/-43/-44). RESULTS: HPV DNA was detected in 58.6% of the 2,543 patients and in 65.2% of the 184 Group I, in 81.7% of 469 Group II, and in 89.9% of Group III. The most frequently found genotypes in all HPV-positive cases were HPV-16, HPV-51, HPV-52, and HPV-58. HPV-16 was the most prevalent type in within Group I and Group III, whereas HPV-52 was most frequently found in Group II. Multiple infection was identified in about 13.6% of HPV-positive cases and most of them were that by two different types. HPV-16 was present in the majority of multiple infection cases. CONCLUSIONS: The distribution of HPV genotypes in Korean women was revealed to have differences to that of other regions, showing higher frequencies of HPV-52, HPV-58, and HPV-51. Group III cases were mostly infected by sole HPV-16, suggesting a certain type may become dominant over others as the disease progresses. The HPV oligonucleotide microarray provides useful information on viral genotype in HPV-related cervical lesions.

B형 간염의 신속한 진단을 위한 Kit의 평가

임지애 ( Jee Aee Im ) 대한임상검사과학회 1996 대한임상검사과학회지(KJCLS) Vol.28 No.1

Sarcopenia and Age-Related Changes in Body Composition in Korean Older Persons

Im, Jee-Aee,Kim, Sang-Hwan,Lim, Hee-Jung,Kim, Chang-Hee,Suh, Sang-Hoon The Korean Society for Biomedical Laboratory Scien 2008 Journal of biomedical laboratory sciences Vol.14 No.1

We examined the prevalence of sarcopenia and age-related changes in body composition in Korean older persons. Community dwelling 77 men and 65 women($60{\sim}88\;yr$) were recruited for this study. Fat-free mass and the percent body fat were determined using bioelectrical impedance analysis. Isometric grip strength was measurement using grip strength dynamometer. Serum levels of fasting glucose, total cholesterol, HDL-cholesterol, and triglyceride were assayed. The prevalence of sarcopenia was found to increase with aging (men in their sixties 82.6%, seventies 96.6% and eighties 100%, and women in their sixties 47.4%, seventies 63%). In both gender groups, handgrip strength was inversely correlated with age and positively correlated with height, lean mass, and fat free mass. Better handgrip strength was related with higher weight in the men and with lower heart rate in the women. Fat free mass, age, and gender were found to be independent factors significantly associated with handgrip strength in the multivariate analysis. In summary, results of the present study suggest that handgrip strength is well associated with age, gender, and fat free mass and the prevalence of sarcopenia is increased with age and is exceeded by 40% among Korean persons older than 60 years.

Kinetics of Goblet Cells and Mast Cells in the Intestine of C3H/HeN and BALB/c Mice Infected with Echinostoma hortense

Im, Jee-Aee,Kim, Insik,Jo, Yoon-Kyung,Lee, Kyu-Jae,Ryang, Yong-Suk THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.2

Mast cells and goblet cells have the ability to protect against parasites by increasing mucus production that traps and excludes worms and prevents their intimate contact with the gut mucosa in the host. In this study, we investigated the function of mast cells and goblet cells for the rejection of Echinostoma hortense (E. hortense). In addition, we used both C3H/HeN and BALB/c mice in order to examine whether mast cells and goblet cells function differentially according to the strains of mice. After an oral infection with 30 E. hortense metacercariae, the number of mucosal mast cells and goblet cells, as well as worm recovery rate, were observed in experimentally infected mice between 1 week and 8 weeks post-infection (PI). Worm recovery rates in C3H/HeN and BALB/c mice were 65.7% and 23%, respectively, in week 1 P.I., indicating that worm expulsion in C3H/HeN mice was higher than in BALB/c mice. Our results demonstrate that the period (week 3 P.I.) in which worm recovery falls rapidly is the same period that the number of goblet cells and mast cells reaches a peak. These results indicate that worm recovery significantly correlates with the growth rate of goblet cells and mast cells (P=0.0482). However, worm expulsion is not associated with goblet cells or mast cells in BALB/c mice.

Effect of Non-Alcoholic Fatty Liver Disease on Components of Metabolic Syndrome in Post-menopausal Women

Moon-Jung Shim,Jee-Aee Im 대한의생명과학회 2008 Biomedical Science Letters Vol.14 No.4

Non-alcoholic fatty liver disease (NAFLD) is associated with various metabolic abnormalities, including central obesity, type 2 diabetes, dyslipidemia, and high blood pressure. This suggests that NAFLD may represent the hepatic manifestation of the metabolic syndrome. In this study, we investigated unfavorable effects NAFLD on components of metabolic syndrome in post-menopause women. Eight hundred sixty-nine postmenopausal women were recruited for this study. The diagnosis of fatty liver was based on the results of abdominal ultrasonography. Serum levels of fasting glucose, total cholesterol, triglyceride, and HDL-cholesterol were measured. The prevalence of component of metabolic syndrome such as hypertension, hyperglycemia, hypertriglyceridemia, and low-HDL-cholesterol was significantly higher in subjects with NAFLD as compared with those without NAFLD. The moderate to severe grade of NAFLD presented higher levels of serum fasting glucose, fasting insulin, HOMA-IR, total cholesterol, and triglycerides than the mild NAFLD and the normal group. In conclusion, metabolic syndrome risk was increased in post-menopause women with NAFLD as compared with those without NAFLD. The severity of NAFLD affected metabolic syndrome risk factors. The optimal strategy for the treatment of NAFLD is likely to include lifestyle modifications and therapy to improve insulin resistance.