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Sodium Iodide Symporter (NIS)를 이용한 분자영상
조제열 대한핵의학회 2004 핵의학 분자영상 Vol.38 No.2
Radioiodide uptake in thyroid follicular epithelial cells, mediated by a plasma membrane transporter, sodium iodide symporter (NIS), provides a first step mechanism for thyroid cancer detection by radioiodide injection and effective radioiodide treatment for patients with invasive, recurrent, and/or metastatic thyroid cancers after total thyroidectomy. NIS gene transfer to tumor cells may significantly and specifically enhance internal radioactive accumulation of tumors following radioiodide administration, and result in better tumor control. NIS gene transfers have been successfully performed in a variety of tumor animal models by either plasmid-mediated transfection or virus (adenovirus or retrovirus)-mediated gene delivery. These animal models include nude mice xenografted with human melanoma, glioma, breast cancer or prostate cancer, rats with subcutaneous thyroid tumor implantation, as well as the rat intracranial glioma model. In these animal models, non-invasive imaging of in vivo tumors by gamma camera scintigraphy after radioiodide or technetium injection has been performed successfully, suggesting that the NIS can serve as an imaging reporter gene for gene therapy trials. In addition, the tumor killing effects of I-131, ReO4-188 and At-211 after NIS gene transfer have been demonstrated in in vitro clonogenic assays and in vivo radioiodide therapy studies, suggesting that NIS gene can also serve as a therapeutic agent when combined with radioiodide injection. Better NIS-mediated imaging and tumor treatment by radioiodide requires a more efficient and specific system of gene delivery with better retention of radioiodide in tumor. Results thus far are, however, promising, and suggest that NIS gene transfer followed by radioiodide treatment will allow non-invasive in vivo imaging to assess the outcome of gene therapy and provide a therapeutic strategy for a variety of human diseases. (Korean J Nucl Med 38(2):152-160, 2004)
NQO1 is Required for β-Lapachone-Mediated Downregulation of Breast-Cancer Stem-Cell Activity
Kim, Dong Wook,Cho, Je-Yoel MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.12
<P>Cancer stem cells (CSCs) exhibit self-renewal activity and give rise to other cell types in tumors. Due to the infinite proliferative potential of CSCs, drugs targeting these cells are necessary to completely inhibit cancer development. The β-lapachone (bL) compound is widely used to treat cancer development; however, its effect on cancer stem cells remain elusive. Thus, we investigated the effect of bL on mammosphere formation using breast-cancer stem-cell (BCSC) marker-positive cells, MDA-MB-231. MDA-MB-231 cells, which are negative for reduced nicotinamide adenine dinucleotide phosphate (NAD(P)H):quinone oxidoreductase (NQO1) expression, were constructed to stably express NQO1 (NQO1 stable cells). The effect of bL on these cells was evaluated by wound healing and Transwell cell-culture chambers, ALDEFLUOR assay, and mammosphere formation assay. Here, we show that bL inhibited the proliferative ability of mammospheres derived from BCSC marker-positive cells, MDA-MB-231, in an NQO1-dependent manner. The bL treatment efficiently downregulated the expression level of BCSC markers cluster of differentiation 44 (CD44), aldehyde dehydrogenase 1 family member A1 (ALDH1A1), and discs large (DLG)-associated protein 5 (DLGAP5) that was recently identified as a stem-cell proliferation marker in both cultured cells and mammosphered cells. Moreover, bL efficiently downregulated cell proliferation and migration activities. These results strongly suggest that bL could be a therapeutic agent for targeting breast-cancer stem-cells with proper NQO1 expression.</P>
Gel-based proteomics in disease research: Is it still valuable?
Kim, Yong-In,Cho, Je-Yoel Elsevier 2019 Biochimica et biophysica acta. Proteins and proteo Vol.1867 No.1
<P><B>Abstract</B></P> <P>Gel electrophoresis had been the primary method in proteomics. In the early era of proteomics, gel electrophoresis was a dominant technique of sample preparation for mass spectrometry analysis. Particularly, two-dimensional electrophoresis provided high-resolution proteome separation, and was regarded as the standard methodology for the separation of wide-range proteomes. However, gel electrophoresis turned downwards due to the progress of other separations including liquid chromatography and ionization techniques, resulting gel-free proteomics finally becoming dominant players at present. There are numerous advantages in gel-free approach in aspects of current trends of disease research. Interestingly, gel-free approaches are still advanced, it seems that gel electrophoresis will not be disappeared. The unique features of gel electrophoresis can be complementary for gel-free and it is suitable for the new wave of top-down functional proteomics.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Throughput of gel-free proteomics is overwhelmed against gel-based proteomics. </LI> <LI> 2-DE is still a meriting technology to provide the highest resolution on protein level. </LI> <LI> Gel-based proteomics is powerful strategy for top-down proteomics. </LI> <LI> Gel-based and gel-free approaches are complementary, not alternative. </LI> </UL> </P>
소(우(牛))의 식도구 평활근 절편에 대한 catecholamine의 작용
조제열,양일석,Cho, Je-yoel,Yang, Il-suk 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.2
Effects of catecholamines were investigated on isolated strips of the male cattle oesophageal groove. In the circular muscles of the bottom and longitudinal muscles of the lip. isometric tensions was recorded with isometric myograph in 25ml organ bath. The results were as follows: 1. The muscular activity was different in preparations from the two parts. In the longitudinal muscle from the lip, rhythmic contractions generally occurred. while in the circular muscle from the bottom they were not seen almost. 2. In the circular muscle of the bottom, the increased tone and biphasic contractions were caused by catecholamines. And these contractions were mediated through $\alpha$-excitatory adrenoceptor. Also circular muscle showed minor inhibitory response to catecholamines. And these effects were mediated through $\beta$-inhibitory adrenoceptor. But the circular muscle was more sensitive to the $\alpha$-excitatory effect than $\beta$-inhibitory effect. 3. In logitudinal muslce of the lip. rhythmic contractions were reduced or disappeared by catecholamines(especially propranolol) and these effects were mediated through $\beta$-adrenoceptor.
Comparative oncology: overcoming human cancer through companion animal studies
Oh Ji Hoon,Cho Je-Yoel 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Comparative oncology is a field of study that has been recently adopted for studying cancer and developing cancer therapies. Companion animals such as dogs can be used to evaluate novel biomarkers or anticancer targets before clinical translation. Thus, the value of canine models is increasing, and numerous studies have been conducted to analyze similarities and differences between many types of spontaneously occurring cancers in canines and humans. A growing number of canine cancer models as well as research-grade reagents for these models are becoming available, leading to substantial growth in comparative oncology research spanning from basic science to clinical trials. In this review, we summarize comparative oncology studies that have been conducted on the molecular landscape of various canine cancers and highlight the importance of the integration of comparative biology into cancer research.