사람의 간내담관의 발달에 관한 형태학적 및 면역조직학적 연구

서연림,지제근 대한병리학회 1994 Journal of Pathology and Translational Medicine Vol.28 No.1

회충란에 의한 간피막의 섬유석회성 결절 1예 보고

서연림,박소영,지제근 대한병리학회 1992 Journal of Pathology and Translational Medicine Vol.26 No.4

사람 위장관의 발달에 관한 형태학적 연구

서연림,지제근 대한병리학회 1990 Journal of Pathology and Translational Medicine Vol.24 No.2

産褥期 感染 誘發 細菌의 生育을 억제하는 韓藥材 探索에 關한 硏究

임재연,성연수,김희진,이태균 동국대학교 한의학연구소 1997 東國韓醫學硏究所論文集 Vol.6 No.1

産褥期 感染이란 分娩後 生殖器의 細菌에 의한 感染을 意味하며, 産後 體溫上昇의 大部分은 骨盤感染에 의한 것으로 産褥期 感染의 重要한 指漂가 된다. 한의학에서 産後發熱의 病因病耭는 주로 邪毒感染, 血瘀, 外感, 血虛 등으로 보았으며, 치료는 一般的으로 産後疾患에는 處證이 많아 發表攻裏를 過度하게 하지 않고, 氣血과 營衛의 調和에 위주로 하나, 外感과 裏實證을 疏怒히 하면 안된다. 따라서 産後發熱을 惹起하는 各種 原因菌에 對한 客觀的인 抑制效果의 檢證은 産後發熱의 治療率을 높이는데 중요하다. 그 결과 산욕기감염을 유발하는 各種 細菌에 對해 黃連, 烏梅, 五味子 등의 水溶性 抽出物이 杭菌活性이 相對的으로 좋은 效果를 보였다. 따라서 産褥期 感染의 治療에 이들 韓藥材를 이용하면 보다 效果的일 것으로 생각된다. Deparment of Oriental Medicine, Graduate School of Dongguk University, Major in Oriental Ob & Gy. Various kinds of medicinal herbs and prescribed herb medicines which have usually been used for treatment of reducing fever, purging intense heat, and detoxication were secreened for antibacterial activity against Proteus vulgaris, S. aureus, and E. faecalis which causing puerperal infection. Minimal inhibitory concentrations of water- and ethanol-soluble extracts of Hwangyon(C. japonica), Omae(P. mume), and Omiza(S. chinensis) were determined and using thin-layer chromatography the extracts of Hwangyon and Omiza were separated. The results obtained were as follows: 1. The water-soluble extracts of Keumeunhwa(L. japonica), Chiza(G. jasminoides), Hwangyon(C. japonica), Dansam(S. miltiorrhiza), Omae(P. mume) and Omiza(S. chinensis) showed antibacterial activities against P. vulgaris ; among them, the extracts of Hwangyon, Omae, and Omiza showed high antibacterial activities. The water-soluble extracts of Keumeunhwa(L. japonica), Hwangyon(C. japonica), Hwangkeum(S. baicalensis), Hwangbaek(P. amurense), Dansam(S. miltiorrhiza), Daehwang(R. coreanum), Omae(P. mume), Omiza(S, chinensis), Gingseng(P. ginseng), and Gamcho(G. uralensis) showed antibacterial activities against S. aureus ; among them, the extracts of Hwangyon and Omiza showed high antibacterial activities. The water-soluble extracts of Banggi(C. trilobus), Daehwang(R. coreanum), Omae(P. mume), Omiza(S. chinensis), and Gamcho(G. uralensis) inhibited the cell growth of E. faecalis ; among them, Omae and 0miza showed high antibacterial activities. The extract of Hwangyon(C. japonica) and Omae(P. mume) showed no inhibition against E. coli. However, the extract of Omiza(S. chinensis) inhibited cell growth of both E. coli and B. subtilis. 2. The water- and ethanol-soluble extract of Hwangyon(C. japonica) showed antibacterial activities against P. vulgaries and S. aureus, those of Omae(P. mume) against P. vulgaris and E. faecalis, and those of Omize(S. chinensis) against all species tested, P. vulgaris, S. aureus, and E. faecalis. With the exception that ethanol-soluble extract of Hwangyon(C. japonica) showed much higher antibacterial activity against S. aureus than water-soluble one, antibacterial activities of both water- and ethanol-soluble ones were similar to each of other two medicinal herbs. 3.When the prescribed herb medicines were tested, Sambohwan showed antibacterial activities against P. vulgaris and S. aureus. No prescribed herb medicine inhibited the cell growth of E. faecalis. 4. Minimal inhibitory concentrations(MICs) of both water- and ethanol-soluble extracts of Hwangyon (C. japonica), Omae(P. mume), and Omiza (S. chinensis) against P. vulgaris were 2.5㎎/㎖, 10㎎/㎖, and 20㎎/㎖, respectively. Those of both Hwangyon (C. japonica) and Omiza(S. chinensis) against S. aureus were 1.25㎎/㎖ and 10㎎/㎖, respectively. MICs of water-soluble extracts of Omae(P. mume) and Omiza(S. chinensis) aganist E. faecalis were 2.5㎎/㎖ and 5㎎/㎖, and those of ethanol-soluble extracts were 5㎎/㎖ and 10㎎/㎖, respectively. Except for those of E. faecalis, the cell growth of P. vulgaris and S. aureus were inhibited by much lower concentration of ethanol-soluble extracts used. As a result, the antibacterial compounds against P. vulgaris, S. aureus, and E. aecalis are contained in the extracts of Hwangyon(C, japonica), 0mae(P. mume), 0miza(S. chinensis), the prescribed herb medicine, Sambohwan, and might be used for treatment of puerperal infection. Further study should be carried out to identify which compounds affect the cell growth inhibition of P. vulgaris, S. aureus, and E. faecalis.

서울시민의 수돗물 불소화에 대한 인식

장재연,조수남,윤제용 대한상하수도학회 2003 상하수도학회지 Vol.17 No.2

Public perception concerning water fluoridation and influencing factors were assessed. A mailing survey was conducted in 1999 with a representative sample of the general Seoul citizen (response rate=40.1%), who consisted of 1108 adults aged between 20 and 78. The data were analyzed using the chi-square test and structural equation modeling. 48.3% favored and 11.4% opposed water fluoridation while 40.5% were not certain. The contact on information about water fluoridation was the most significantly influencing factor on the perception concerning water fluoridation while experience of marriage, income and the perception of tap water were also significant. 52.8% expect governmental health authorities to take the decisions about water fluoridation.

랫드에서 l-muscone의 급성독성 및 아급성독성시험 연구

오승민,연제덕,남혜운,박대규,조명행,정규혁 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

l-Muscone is synthesized for use as substitutive material of musk which is the active ingredient of woohwangchungsimwon. The objective of this investigation was to evaluate the acute and subacute toxicity of l-muscone in rats. In oral acute toxicity test, SPF Sprague-Dawley male and female rats were gavaged with l-muscone of two doses(0, 5.0g/㎏). No dead animal and abnormal autopsy findings were found in control and treated group. Body weights were slightly decreased in both sexes of rats treated with 5.0g/㎏. Therefore, oral LD_50 of l-muscone was consider to be higher than 5.0g/㎏ in male and female rats. In intraperitoneal acute toxicity test, rats were injected intraperitoneally with dosages of 0, 1,000, 1,316, 1,732, 2,279 and 3,000 ㎎/㎏. Decreased body weights and motor activities were observed at high dose group. Intraperitoneal LD_50 of l-muscone were 1,920 ㎎/㎏ in male and female rats. In the subacute study. l-muscone was administrated orally to both sexes of rats for 4 weeks as several doses(0, 10, 100 and 1,000 ㎎/㎏). There were neither dead animals nor significant changes of body weights during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, urinalysis, hematology, serum biochemical analysis, and other findings. Above data suggest that no observed adverse effect level of l-muscone in rats might be over 1,000 ㎎/㎏/day in this study.

정신과 입원환자의 서비스 만족척도의 개발

김철권,이지연,송영선,김규호,김경률,김제원,이동기,최병무 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.3

목적 본 연구의 목적은 국내 정신의료기관(정신병원, 종합병원 정신과)에서 간편하게 사용할 수 있는 신뢰도와 타당도가 검증된 입원환자의 서비스 만족척도를 개발하기 위한 것이다. 방법 여러 단계를 거쳐 국내 실정에 맞는 문항을 개발하였고,정신병원, 종합병원, 대학병원 정신과 병동에서 퇴원하는 348명의 환자들을 대상으로 신뢰도와 타당도를 조사하였다. 요인분석 결과 5개의 요인이 추출되었으며 전체 변량의 63.04%를 설명하였다. 내적 일관성에 대한 신뢰도 계수 (Crohnbach's α)는 0.95로 상당히 높은 수준이었고, 수정된 개별문항-총점 상관계수는 0.50부터 0.72까지의 범위에 속하였다. 요인분석을 통해 추출된 서비스 만족척도의 각 하위척도 점수와 서비스 만족척도에 첨부된 각 영역에 대해 전반적인 만족도를 묻는 세 개의 문항 점수 간의 상관성 역시 유의하게 높았다. 결과 진단명에 따른 서비스 만족척도의 전체 점수에서는 불안/신체형/강박장애 환자군과 기분장애 환자군이 각각 정신분열병 및 기타 정신병 환자군과 알코올중독 장애 환자군에 비하여 유의하게 높은 점수를 보였으며, 입원형태에 있어서는 자발적 입원군이 강제 입원군에 비하여 전체 만족점수가 유의하게 높았다. 그러나 성별, 월수입, 학력, 직업, 종교, 결혼상태 등의 변수에서는 서비스 만족척도의 전체 점수에서 유의한 차이가 발견되지 않았으며, 마찬가지로 연령, 입원일수, 입원횟수, 첫 발병나이, 유병기간 등의 임상적 변수에서도 서비스 만족척도의 전체 점수와 유의한 관계를 보이지 않았다. 서비스 만족척도의 전체점수에서 대학병원 환자군이 정신병원 환자군과 종합병원 환자군에 비하여 각각 유의하게 높았다. 결론 결론적으로 국내 정신의료기관에 입원한 환자들의 서비스 만족을 평가하기 위한 목적으로 개발된 본 척도는 높은 수준의 신뢰도와 타당도를 보였으며, 또 국내 정신의료 환경에 맞는 요인구조를 보였다. 따라서 정신의료 서비스에 대한 정신과 환자의 만족도에 대한 연구가 거의 없는 실정에서 본 척도의 개발은 향후 국내 정신의료의 질과 치료결과를 높이는 도구로 활용될 수 있을 것으로 기대된다. Objectives : To develop and test the validity and reliability of a brief self-completed questionnaire (Service Satisfaction Scale : SSS) for routinely assessing the quality of service in psychiatric ward inpatients. Methods : A 30-item multidimensional questionnaire was developed by several steps of face validity and content validity. The questionnaire was administered to inpatients (n=348) discharged from psychiatric hospitals, general hospitals, and University hospitals. Construct validity was supported by performing principal component analysis. Reliability was estimated by calculating internal consistency of Cronbach's alpha. Results : Factor analysis yielded five factors comprising staff attitude, treatment quality, ward environment, access/cost, and ward rule, which account for 63.04% of the common variance. The internal consistency of the scale was high (Cronbach's alpha=0.95). The concurrent validity was supported by the significant correlation of each of five factors with item that measured overall satisfaction of SSS. Patients with neurosis (anxiety disorder, somatoform disorder, obsessive compulsive disorder) and mood disorders were significantly satisfied than those with psychosis and alcoholic disorders. Patients who admitted voluntarily were more significantly satisfied than those who admitted involuntarily. Sociodemographic variables such as age, gender, marital status, monthly income, education level, employment status and religion were not significantly different at the total scores of SSS. Similarly, clinical characteristics such as age of onset, duration of illness, lengths of hospital stay and number of previous hospitalization did not associate significantly with the total scores of SSS. Patients discharged from university hospitals were significantly more satisfied than those of the general and psychiatric hospitals. Conclusion : SSS performed well in the validity and reliability, indicating that it can be a useful tool for measuring Satisfaction of psychiatric inpatients in Korea.

16번 삼염색체증 마우스에서 관찰된 이상 뇌발달과 세포자멸사

조은윤,서연림,지제근 대한병리학회 1999 Journal of Pathology and Translational Medicine Vol.33 No.8

비·부비동에 발생한 모균증 : 3례보고

최영석,진흥률,연제엽 충북대학교 의과대학 충북대학교 의학연구소 2005 忠北醫大學術誌 Vol.15 No.1

Sinonasal mucormycosis is a form of invasive fungal infection of the sinonasal track that often extends to the orbit, brain, and palate. It is caused by the order Mucorales and commonly associated with immuconcompromised conditions such as diabetic ketoacidosis, HIV infection, hematologic malignancy. Treatment includes aggressive debridement, systemic antifungal therapy and control of underlying factors. We experienced 3 cases of sinonasal mucormycosis: rhino-orbital, isolated sphenoid sinus, palatal mucormycosis. Each patients had undergone wide surgical debridement including a case with orbital exenteration and systemic antifungal therapy. We present our successful management with literature reviews. 비·부비동에 발생하는 모균증은 안와, 두 개내, 또는 구개 침범이 흔한 진균성 감염이고 mucorales종의 일종으로 당뇨병케토산증이나 AIDS환자, 혈액 악성종양과 같이 면역이 저하된 환자에게서 주로 발생한다. 치료로는 환자의 원인 요소를 치료하고 광범위한 수술적 절제와 장기간의 항진균제의 사용이 이용되고 있지만 치료효과가 만족하지 못하다. 저자들은 안구를 침범하여 안구를 제거한 경우, 장기간의 두통을 호소한 환자에게서 발견된 접형동 단독의 감염 사례, 그리고 구개를 침범하여 구개의 일부를 절제한 사례를 경험하였고 광범위한 감염부위의 절제와 장기간의 항진균제 치료를 이용하여 완치하였기에 문헌 고찰과 함께 보고하고자 한다.

Mycophenolic Acid와 Rapamycin이 흰쥐 사구체 혈관간세포증식과 세포외기질 생성에 미치는 영향

김명수,박제현,하헌주,허규하,서지연,김유선,김혜진,박기일 이화여자대학교 약학연구소 2004 藥學硏究論文集 Vol.- No.14

Background: Excess proliferation and extracellular matrix (ECM) accumulation of mesenchymal cells such as vascular smooth muscle cells (VSMC) and glomerular mesangial cells cause chronic allograft nephropathy showing transplant vascular sclerosis and glomerulosclerosis. Mycophenolic acid (MPA) and rapamycin (RPM) are well known as strong inhibitors of VSMC proliferation, but their effects on the glomerular mesangial cells are not yet clearly understood. This study examined the effects of MPA or RPM on PDGF-induced proliferation and ECM accumulation in rat glomerular mesangial cells. Methods: Mesangial cells isolated from the glomeruli of Sprague-Dawley rats were cultured with DMEM containing 20% fetal bovine serum. Growth arrested and synchronized cells were administered with test drugs (MPA10 nM-10μM, RPM 0.1 nM-1μM) before the addition of PDGF 10 ng/mL. Cell proliferation was assessed by [³H]thymidine incorporation, collagen by [³H]proline incorporation, and fibronectin, ERK, and p38 MAPK by Western blot analysis. Results: PDGF increased mesangial cell proliferation by 4.64-fold. Compared to stimulated control, MPA above 500 nM and RPM above 10nM showed a significant inhibitory effect in a dose-dependent manner. The IC_(50) of MPA and RPM against PDGF-induced mesangial cell proliferation were around 500 nM and 100 nM, respectively. The collagen synthesis was also inhibited by MPA and RPM, but the fibronectin secretion was inhibited by MPA alone. The proliferation of mesangial cell correlated with activation of ERK and. p38 MAPK. MPA, but not RPM, inhibited ERK and p38 MAPK activation. Conclusion: This study demonstrated that MPA and RPM significantly inhibited PDGF-induced proliferation and ECM production in rat glomerular mesangial cells. The inhibitory effects of MPA, but not RPM, are correlated with ERK and p38 MAPK.