Pulmonary inflammation caused by silica dioxide nanoparticles in mice via TXNIP/NLRP3 signaling pathway

Je‑Oh Lim,Je‑Won Ko,Tae‑Yang Jung,Woong‑Il Kim,So‑Won Pak,In‑Sik Shin,Won‑Kee Yun,Hyoung‑Chin Kim,Jeong‑Doo Heo,Jong‑Choon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Silica dioxide nanoparticles (SiONPs) have been used for various medical applications, including therapeutics and imaging, and the use of SiONPs has increased gradually over the years. However, despite an increase in the use of SiONPs, not much is known about mechanism of action of SiONPs and their pulmonary toxicity. Objective The present study investigated the pulmonary toxicity of SiONPs and explored the underlying mechanism of action, primarily focusing on thioredoxin-interacting protein (TXNIP)/NOD-like receptor pyrin domain-containing 3 (NLRP3) in SiONPs-treated mice. We investigated the toxic effects of SiONPs in the lung of BALB/c mice administered 5, 10, and 20 mg/kg SiONPs for 3 days. Results Exposure to SiONPs markedly increased inflammatory cell counts, including those of neutrophils and macrophages, and levels of inflammatory mediators, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in a dose-dependent manner in the bronchoalveolar lavage fluid. Moreover, the inflammation was verified upon histopathological analysis. In addition, exposure to SiONPs increased the expression of TXNIP in a dose-dependent manner and, in turn, upregulated NLRP3 inflammasome proteins, which subsequently induced IL-1β production. Conclusion Collectively, exposure to SiONPs induced inflammation in the lungs of mice, which resulted in the activation of IL-1β production via the TXNIP-NLRP3 axis. Our results provide useful information on the pulmonary toxicity induced by SiONPs and provide insights into the underlying mechanism of action.

Girder의 강성을 고려한 복합 재료 교량 상판의 구조 거동

원치문,박제선,이정호,심도식 江原大學校 産業技術硏究所 1998 産業技術硏究 Vol.18 No.-

Many of the bidge and building floor systems, including the girders and cross-beams, also behave a similar special orthotropic plates. Such plates are subject to the concentrate masses in the form of traffic loads, or the test equipments such as the accelerator in addition to their own masses. Analysis of such problems is usually very difficult. Most of the bridge slabs on girders have large aspect ratios. Finite difference method is used for this purpose, in this paper. The reuslt is compared with that of the beam theory.

모바일 인터넷 환경에 따른 M-Commerce 확산에 관한 논의

전창원,이제홍 동국대학교 경제경영연구원 무역연구부 2000 國際貿易硏究 Vol.24 No.2

野冬靑果가 實驗的 糖尿에 미치는 影響에 관한 免疫組織化學的 硏究

柳濟原,李學仁,金蓮燮 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1

This experiment was performed into investigate the effect of Syzygii cumini Fructrs on the diabetic rats induced by STZ. Experimental diabetes was induced by 55㎎/㎏ of STZ. injection and administered Syzygii cumini Fructrs extract during 14days after STZ injection. Glucagon and insulin granules in Langerhans islets were stained by use of immunohistochemical method and observed the relative amount of the each granules in Langerhans islet by light microscope and image analysis ststem. The results obtained are as follows: 1. Area % of insulin granules in Langerhans islets in Syzygii cumini Fructrs raised and showed the statistically significant difference with the control group at 14th day. 2. Area % of glucagon granules in Langerhans islets in Syzygii cumini Fructrs decreased and showed the statistically significant difference with the control group at 7th and 14th day. To conclude, it can be inferred that Syzygii cumini Fructrs has a control effect on glucagon and insulin granules in Langerhans islets of diabetic rats induced by STZ.

우심방과 우심실의 거대 심장 혈관종 1예

김제열,조상호,김건일,허경림,김현숙,조구영,최영진,이원용,임종윤 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Cardiac tumors, especially the primary tumors involving any part of the heart are extremely rare and its relative incidence has been reported to be approximately 0.02%. We report a patient with huge cardiac hemangioma who complained of shortness of breath, general weakness, and dizziness, Imaging study by echocardiography and computed tomography of thorax revealed a huge lobulating mass like a bunch of grapes in the right chamber of heart. The surgical exploration of thorax was performed and a histological diagnosis of spindle cell hemangioma was obtained by microscopy. The patient was treated by surgical resection of the tumor and doing well after surgery. Our experience indicated that prompt diagnosis and treatment of cardiac hemangioma is imperative for patients' prognosis.

북한 벼 품종의 지대별 출수반응

양원하,김덕수,전용희,조영찬,김제규,이문희 한국국제농업개발학회 2004 韓國國際農業開發學會誌 Vol.16 No.1

북한벼의 출수 반응을 검토하기 위하여, 작물시험장 인공기상실과 표고가 다른 수원, 철원, 진부 등 3 지역에서 이앙기를 달리하여 1998년과 1999년에 시험 수행한 결과를 요약하면 다음과 같다. 가. 지대별 이앙기 변동에 다른 출수일수 변화는 표고가 낮은 지역일수록, 이앙기가 늦어질수록 출수일수가 짧아졌으며, 지역과 이앙기 이동에 따른 경향을 살펴보면, 표고가 높은 진부지역에서 이앙기가 늦어질수록 출수일수가 단축되는 정도가 뚜렷하게 나타났다. 2. 북한 벼의 기본영양생장성은 조생종은 대조 품종인 오대벼보다 짧은 편이었고, 중생종은 오대벼에 비하여 긴 편이었다. 다. 감온성은 대비품종인 오대벼보다 대체로 예민한 것으로 나타났으며 극조생종인 올벼 2호는 감온성이 매우 예민한 반응을 보여 ′98년에는 42일, ′99년에는 39일이었다. The experiment was carried out to determine heading response of North Korean rice cultivars at NCES(national crop experiment station). RDA. South Korea in 1998 and 1999. This experiment was tested at three different locations which were Suwon, Cheolwon and Jinbu with 5 transplanting times with 10 cultivars. Duration of heading stage of the rice plant was shortened at lower altitude and in later transplanting date, respectively. Bases on the heading days, it was classified as the basic vegetative phase(BVP) and thermo-sensitive phase(TSP) in tested cultivars. BVPs and TSPs among treated varieties were grouped with four groups. BVPs of North Korean rice cultivars for early maturity were shoter than that of Odaebyeo of Korean rice cultivars, however, those for medium maturity were longer than that of the control cultivar, Odaebyeo. Meanwhile, TSPs of North Korean rice cultivars were more sensitive than that of the check cultivar of Odaebyeo, Correlation between 1998 and 1999 was more significant in basic vegetative phase than in thermo-sensitive stage. Therefore, the heading time of the rice plant primarily depended on the period of basic vegetative phase growth.

물라이트로 부터 용융 알루미늄의 치환 반응에 의한 Al₂O₃-Al 복합체의 제조

배원태,김용진,김승제 慶尙大學校生産技術硏究所 1996 生産技術硏究所論文集 Vol.12 No.-

Al₂O₃-Al composites were produced by displacement reaction method, which was carried out by immersing the mullite preform which was prepared by cutting and polishing the commercial sintered mullite in molten aluminum. The change of physical and mechanical properties such as bulk density, porosity, water absorption, 4-point MOR, hardness, fracture toughness of Al₂O₃-Al composites with the variation of the reaction temperature and time were investigated. Microstructure and the phases of this composite were also investigated by SEM and XRD analysis. A1₂O₃-Al composites which was produced at 1000℃ for 24 hours showed porosity of 2.07%, water absorption of 0.35%, bulk density of 3.43g/cm3, 4-point MOR of 287.97MPa, hardness of 593.6kg/mm2 and ?? of 8.64MPa·??. The microstructure of this composites showed three dimensionally co-continuous of alumina which provides wear resistance and high stiffness and aluminum which acts as a toughening phase. The exact shape of the preform is retained and a net-shaped A1₂O₃-Al composite was produced.

경도 및 중등도 근시환자에서 PRK 후 교정법에 따른 인공수정체 도수 비교

신제원,김중곤,송병주 한양대학교 의과대학 2002 한양의대 학술지 Vol.22 No.1

To compare IOL powers which are calculated by post-op keratometric(K) values and refraction derived K values, and by refraction corrected method, respectively, in patients who have been treated with excimer laser photorefractive keratectomy(PRK) for mild to moderate myopia. A total of 38 consecutive eyes of 23 patients who were treated with PRK for mild to moderate myopia have been followed up for more than 6 months. IOL powers from SRK/T formula by post-op K values and refraction derived K values were compared with IOL powers by refraction corrected method. The mean values of IOL powers using post-op K values, refraction derived K values, and refraction corrected method were 20.69±0.93(18.21 to 22.93) diopter(D), 20.81±0.88 D(18.60 to 22.43), and 21.26±0.79 D(19.08 to 23.03) respectively. There was no significant statistical difference between the mean values of IOL powers using post-op K values and refration derived K values (p〉0.05). However, the mean value of IOL powers by refraction corrected method was statistically higher than other two values(p〈0.05). In patients with excimer laser PRK treatment for mild to moderate myopia, IOL power using refraction corrected method was statistically higher than IOL power using post-operative K values and refraction derived K values.

수면주기 지연 증후군 1례

박원명,김제헌 大韓神經精神醫學會 1992 신경정신의학 Vol.31 No.1

Whereas insomnia (disorders of initiating and maintaining sleep) and hypersomnia(disorders of excessive somnolence) have been studied for many centuries, physicians only recently become aware of that the sleep-wake rhythm itself can also be disturbed. Indeed, at this time we can only dimly perceive the importance of circadian rhythm for healthful sleeping. Not only do our internal circadian rhythm have to be properly entrained to the 24-hour cycling of this planet, but it also seems crucial that an individual's usual bedtime hours be properly located within that individual's circadian rhythm. Delayed sleep phase syndrome is one of disorders of the sleep-wake schedule, which usually arises from mismatches between the patient's circadian rhythm and society's timetable. We experienced a case of salary man who developed delayed sleep phase syndrome for about one year. He had considerable difficulty in falling asleep at appropriated time in the night and increasing difficulty in awakening in the next morning. When not on a strict schedule(eg, weekends, holidays), he had a normal sleep pattern and after a sleep of normal length awaked spontaneously and felt refreshed. Polysomnographic recordings performed in our department for 3 consecutive nights demonstrated that he was noted only a delayed pattern of sleep and awaking time. There was no evidence of significant organic diseases except hepatitis type B. Past history revealed no previous psychiatric disorders and no family history of psychiatric disorders.

골다공증 진단에 피부 두께의 임상적 유용성 평가

박원근,최민주,김호찬,조성제,박전홍 제주대학교 공과대학 첨단기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.2

The study was carried out to examine how much clinically useful was the skin thickness in diagnosis of osteoporosis. The project was to look into not only the presumption that patients with osteoporosis have the skin loss at the similar rate of the reduction in the collagen content of the bone but also the correlation between the collagen content and the skin thickness. The skin thickness was measured on the dorsal hand(right) of the patients using an ultrasonic technique with a 20 MHz focused ultrasonic transducer. Measurements of the bone density of the patients were made on both the lumbar spine (L2-4) and the femoral neck using Dual­Energy X­ray Absorptionmeter(DEXA, Norland). An ultrasonic bone densitometer(Mark 6000, Medison Co. Korea) was taken to measure the speed of sound (SOS) on the calcaneus. It was observed that there were some degrees of correlation between the bone density obtained by the DEXA and the SOS of the calcaneous (P<0.01). There was no evidence that the skin thickness was correlated with the DEXA produced bone density and ultrasonically measured SOS.