Relative Expression of Apocarotenoid Biosynthetic Genes in Developing Stigmas of Crocus sativus L.

Javid IqbaLMzr,Raies Ahmad Qadri,Nazeer Ahmed,Tassem Ahmad Mokhdomi,Asrar Hussain Wafai,Sajad Hassan Wani,Shoiab Bukhari,Asif Amin 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.3

Saffron, the desiccated stigmas of Crocus sativus, is recognized for its attractive color, flavor, and aroma which are due to the accumulation of crocin, picrocrocin, and safranal, respectively. HPLC analysis demonstrated maximum apocarotenoid accumulation during the fully developed scarlet stage of stigma development followed by the orange and yellow stages of stigma development. Reverse Transcription-PCR analysis revealed a concurrent expression pattern of CsZCD and CsLYC genes in a developmental stagespecific manner. However, CsBCH and CsGT2 genes were specifically expressed during the mature, scarlet stage of stigma development. Real-Time PCR analysis showed a sharp increase in gene expression of CsLYC gene during stigma development indicative of its possible regulatory role in apocarotenoid biosynthesis or stigma development. Results suggest that genetic manipulation of this gene can help to improve the quality of stigma in saffron; besides highlighting its potential to monitor stigma development during in vitro experimentation

In vivo evaluation of analgesic, anti-inflammatory, and neuropharmacological activities of the chemical constituent from Nepeta clarkei

Javid Hussain,Najeeb Ur Rehman,Ahmed Al-Harrasi,Abdul Latif Khan,Tania Shamim Rizvi,Faryal Vali Mohammad,Mehjabeen,Liaqat Ali 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

The plant species of genus Nepeta are used totreat various human diseases and for ornamental purposesas well. Nepethalate B (1) was isolated as a result ofphytochemical investigations of Nepeta clarkei and wassubjected in the present study for investigation of analgesic,anti-inflammatory, and central nervous system (CNS)depressant activities. The percentage inhibition in phase Iof the acetic acid induced writhing test of compound 1(dose of 0.1, 0.2 and 0.4 mg/kg of body weight) was 53.3,58.0 and 86.7 % respectively. These results were foundsignificantly higher (P\0.01) as compared to the negativecontrol. Moreover, the percentage inhibitions of threephases for 0.1, 0.2 and 0.4 mg/kg were higher than thevalues obtained for Aspirin (positive control). In formalintest, the percentage pain inhibition between 0 and 5 min(early phase) was 68.0, 25.5, and 75.5 % for 0.1, 0.2 and0.4 mg/kg intra-peritoneal doses of compound 1 respectively. In case of late phase (20–30 min) it was 63.0, 66.7and 48.1 %, respectively. In comparison to aspirin, overallpercentage inhibition of compound 1 was significantlyhigher in early and late phases. Interestingly, at all dosescompound 1 showed more potent anti-inflammatory effectsin terms of intensity and duration as compared to aspirin. The gross behavioral study of nepethalate B (1) was alsocarried out and the results revealed that it exhibited CNSdepression in the mice and showed a prominent decrease inlocomotor activity.

Surface energy and wettability control in bio-inspired PEG like thin films

Javid, A.,Kumar, M.,Wen, L.,Yoon, S.,Jin, S.B.,Lee, J.H.,Han, J.G. Elsevier Ltd 2016 Materials & Design Vol.92 No.-

<P>Tailoring of chemical functionalities in polymer films can induce interesting biocompatibility, however the sequential process of polymerization followed by functionalization imposes surface-interface complexities and inhomogeneity of functional groups across the thickness. Here, a single-step plasma process, enabling the simultaneous polymerization-functionalization, is demonstrated to control the surface energy and wettability of polyethylene glycol-like thin films. Chemical studies, carried out by Fourier transform infra-red spectroscopy and X-ray photoelectron spectroscopy, confirm the evolution and enhancement in amide functionalities, owing to the increase in the electronic transitions related to nitrogen based ions/radicals (independently confirmed by optical emission spectroscopy). In present case, the evolution and control over amide functionalities lead to the enhancement in wettability and surface energy tailoring in 60.5-67.5 mJ/m(2) range. Excellent growth of L-929 fibroblast cells is obtained by the synergic contribution of plasma power and N-2 flow rate via enriching the amide functionalities in these films. (C) 2015 Elsevier Ltd. All rights reserved.</P>

Size-controlled growth and antibacterial mechanism for Cu:C nanocomposite thin films

Javid, A.,Kumar, M.,Yoon, S.,Lee, J.,Han, J. Royal Society of Chemistry 2017 Physical chemistry chemical physics Vol.19 No.1

<P>The interdependence of 'size' and 'volume-fraction' hinders the identification of their individual role in the interface properties of metal nanoparticles (NPs) embedded in a matrix. Here, the case of Cu NPs embedded in a C matrix is presented for their profound antibacterial activity. Cu:C nanocomposite thin films with fixed Cu content (approximate to 12 atomic%) are prepared using a plasma process where plasma energy controls the size of Cu NPs (from 9 nm to 16 nm). An inverse relationship between the size-effect on antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria is established through the real time monitoring of an aliquot by inductively coupled plasma mass spectrometry, which confirmed the inverse relationship of Cu ion release from the nanocomposite with varied Cu NP sizes. It was found that enhancing the total power density increases the plasma density as well as effective kinetic energy of the plasma species, which in turn creates a large number of nucleation sites and restricts the island kind of growth of Cu NPs. The mechanism of NP size-control is illustrated on the basis of ion density and nucleation and the growth regime of plasma species. This physical approach to NP size reduction anticipates a contamination-free competitive recipe of size-control to capping based chemical methods.</P>

STRICT COMMON FIXED POINT THEOREMS FOR HYBRID PAIRS OF MAPPINGS VIA ALTERING DISTANCES AND AN APPLICATION

( Javid Ali ),( V. Popa ),( M. Imdad ) 호남수학회 2016 호남수학학술지 Vol.38 No.2

In this paper, we utilize an implicit relation to improve and extend some strict common fixed point results of the existing literature to two pairs of hybrid mappings in 2-metric spaces via altering distances. As an application, we also prove some strict common fixed point theorems for hybrid pairs of mappings satisfy- ing a contractive condition of integral type in 2-metric spaces.

Role of surface-electrical properties on the cell-viability of carbon thin films grown in nanodomain morphology

Javid, Amjed,Kumar, Manish,Yoon, Seokyoung,Lee, Jung Heon,Tajima, Satomi,Hori, Masaru,Han, Jeon Geon IOP 2016 Journal of Physics. D, Applied Physics Vol.49 No.26

<P>Carbon thin films, having a combination of unique physical and chemical properties, exhibit an interesting biocompatibility and biological response to living entities. Here, the carbon films are developed in the morphology form of nano-domains with nanoscale inter-domain separations, tuned by plasma conditions in the facing target magnetron sputtering process. The wettability and surface energy are found to have a close relation to the inter-domain separations. The chemical structure of carbon films exhibited the relative enhancement of sp<SUP>3</SUP> in comparison to sp<SUP>2</SUP> with the increase of domain separations. The cell-viability of these films shows promising results for L929 mouse fibroblast and Saos-2 bone cells, when inter-domain separation is increased. Electrical conductivity and surface energy are identified to play the key role in different time-scales during the cell-proliferation process. The contribution from electrical conductivity is dominant in the beginning of the cultivation, whereas with the passage of time (~3–5 d) the surface energy takes control over conductivity to enhance the cell proliferation.</P>

Study of sterilization-treatment in pure and N- doped carbon thin films synthesized by inductively coupled plasma assisted pulsed-DC magnetron sputtering

Javid, A.,Kumar, M.,Han, J.G. Elsevier BV * North-Holland 2017 Applied Surface Science Vol.392 No.-

Electrically-conductive nanocrystalline carbon films, having non-toxic and non-immunogenic characteristics, are promising candidates for reusable medical devices. Here, the pure and N- doped nanocrystalline carbon films are deposited by the assistance of inductively coupled plasma (ICP) in an unbalanced facing target pulsed-DC magnetron sputtering process. Through the optical emission spectroscopy study, the role of ICP assistance and N-doping on the reactive components/radicals during the synthesis is presented. The N-doping enhances the three fold bonding configurations by increasing the ionization and energies of the plasma species. Whereas, the ICP addition increases the plasma density to control the deposition rate and film structure. As a result, sputtering-throughput (deposition rate: 31-55nm/min), electrical resistivity (4-72Ωcm) and water contact angle (45.12<SUP>o</SUP>-54<SUP>o</SUP>) are significantly tailored. Electric transport study across the surface microchannel confirms the superiority of N-doped carbon films for sterilization stability over the undoped carbon films.

Bioactivity of endodontic biomaterials on dental pulp stem cells through dentin

Javid, Bahar,Panahandeh, Narges,Torabzadeh, Hassan,Nazarian, Hamid,Parhizkar, Ardavan,Asgary, Saeed The Korean Academy of Conservative Dentistry 2020 Restorative Dentistry & Endodontics Vol.45 No.1

Objectives: This study investigated the indirect effect of calcium-enriched mixture (CEM) cement and mineral trioxide aggregate (MTA), as 2 calcium silicate-based hydraulic cements, on human dental pulp stem cells (hDPSCs) through different dentin thicknesses. Materials and Methods: Two-chamber setups were designed to simulate indirect pulp capping (IPC). Human molars were sectioned to obtain 0.1-, 0.3-, and 0.5-mm-thick dentin discs, which were placed between the 2 chambers to simulate an IPC procedure. Then, MTA and CEM were applied on one side of the discs, while hDPSCs were cultured on the other side. After 2 weeks of incubation, the cells were removed, and cell proliferation, morphology, and attachment to the discs were evaluated under scanning electron microscopy (SEM). Energy-dispersive X-ray (EDXA) spectroscopy was performed for elemental analysis. Alkaline phosphatase (ALP) activity was assessed quantitatively. The data were analyzed using the Kruskal-Wallis and Mann-Whitney tests. Results: SEM micrographs revealed elongated cells, collagen fibers, and calcified nucleations in all samples. EDXA verified that the calcified nucleations consisted of calcium phosphate. The largest calcifications were seen in the 0.1-mm-thick dentin subgroups. There was no significant difference in ALP activity across the CEM subgroups; however, ALP activity was significantly lower in the 0.1-mm-thick dentin subgroup than in the other MTA subgroups (p < 0.05). Conclusions: The employed capping biomaterials exerted biological activity on hDPSCs, as shown by cell proliferation, morphology, and attachment and calcific precipitations, through 0.1- to 0.5-mm-thick layers of dentin. In IPC, the bioactivity of these endodontic biomaterials is probably beneficial.

COMMON FIXED POINT THEOREMS FOR L-FUZZY MAPPINGS IN b-METRIC SPACES

Javid Ali,M.A. Ahmed,H.A. Nafadi 한국전산응용수학회 2017 Journal of applied mathematics & informatics Vol.35 No.3

In this paper, we prove common fixed point theorems for Lfuzzy mappings under implicit relation in b-metric spaces. Further, results obtained for an integral type contractive condition. These theorems generalize and improve previous corresponding results.

Cost Effective Design of High Voltage Impulse Generator and Modeling in Matlab

Javid, Zahid,Li, Ke-Jun,Sun, Kaiqi,Unbreen, Arooj The Korean Institute of Electrical Engineers 2018 Journal of Electrical Engineering & Technology Vol.13 No.3

Quality of the power system depends upon the reliability of its components such as transformer, transmission lines, insulators, circuit breakers and isolators. The transient voltage due to internal or external reasons may affect the insulation level of the components. The insulation level of these components must be tested against these conditions. Different studies, testing of different electrical components against high voltage impulses and different industrial applications rely on the international manufactures for pulsed power generation and testing, that is quite expensive and large in size. In this paper a model of impulse voltage generator with capacitive load of pin type insulator is studied by simulation method and by an experimental setup. A ten stage high voltage impulse generator (HVIG) is designed and implemented for different applications. In this proposed model, the cost has been reduced by using small and cheap capacitors as an alternative for large and expensive ones while achieving the same effectiveness. Effect of the distributed capacitance in each stage is analyzed to prove the effectiveness of the model. Different values of front and tail resistances have been used to get IEC standard waveforms. Results reveal the effectiveness at reduced cost of the proposed model.