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파(Alium fistulosum L.)의 회기조직배양으로부터 체세포배 발생
金載焄,蘇雄永 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-
파(Allium fistulosum L.)의 화기를 배양하여 화판과 약편으로부터 유래된 캘러스부터 체세포배를 유도하였고, 이 과정중의 조직학적 특징을 관찰하여 수선형 단자엽 식물의 체세포배발생과정을 구명하고자 본연구를 실시하였다. 황색의 배발생캘러스는 2,4-D(특히 1.0 mg/L)가 첨가된 MS배지에서 유도되었다. 구상배는 배발생캘러스를 0.2 mg/L 2,4-D로 옮겨 주었을 때 세포는 작고, 세포질이 농후한 세포군으로부터 기원되었다. 그러나 배발생캘러스를 사이토키닌이 첨가된 배지에 이식하였을 때에는 부정근이 관찰되었다. 구상배는 정단부가 길게 신장되면서 한개의 자엽이되고, 이 자엽의 기저부 측면의 작게 열개된 홈으로부터 정단분열조직이 발생되었다. 파의 체세포배의 초기 발생과정의 특징을 접합자배 발생과정과 비교하였을 때, 원배시기의 세포분열 패턴이 약간 달랐고, 체세포배에서 배병이 뚜렸하지않는 차이점이 관찰되었다. 그러나 그 이후의 배발달의 형태적 특징은 서로 비슷하였다. Yellowish embryogenic calli formed on the petal and the sepal of Allium fistulosum L. cultured on MS medium supplemented with 1.0 mg/L 2,4-D. Upon transfer onto MS medium with 0.2 mg/L 2,4-D, the calli gave rise to numerous globular embryos. Subsequently they were transferred onto MS basal medium, wherein they developed into cotyledonary embryos. A slight depression on one side of cotyledon base revealed the site of the future shoot apical meristem. The pattern of this somatic embryogenesis at the early stage was similar to that of amaryllid zygotic embryogenesis. However, there were some differences including the absence of suspensor development at the proembryo stage.
참옻나무(Rhus verniciflua)배발생캘러스로부터 체세포배발생에 의한 식물체 재분화
김재훈,이원석,권기원,안준교,최용익,Kim, Jae-Whune,Lee, Won-Seok,Kwon, Ki-Won,In, Jun-Gyo,Choi, Yong-Eui 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.3
Excised cotyledons and embryo axises of zygotic embryos of Rhus vemicifera were cultured on Murashige and Skoog(MS) medium with various concentrations of 2,4-D. About 3-5% of explants produced callus. Embryogenic callus was preferentially induced from basal parts of embryo axis of zygotic embryos seeds when they were cultured without removal of seed coats. Somatic embryos were developed from embryogenic callus in growth regulator-free medium after 2-3 subcultures on medium with 1.0mg/L 2,4-D and these embryos were matured to cotyledonary stage. Plantlets with well-developed shoots and roots from embryos were obtained on $\frac{1}{4}$MS medium with GA$_{3}$. After acclimatization of plantlets on artificial soil, they were exposed to soil pots.
Youn-Soo Cha,Ju-Ryoun Soh,Jae-Whune Kim 한국식품영양과학회 2003 Preventive Nutrition and Food Science Vol.8 No.1
Acanthopanax senticosus was grown by a novel, proprietary method, of culturing isolated cells in a bioreactor. An extract from the cells was evaluated for its effect on lipid metabolism in rats fed a high fat diet. Male Sprague-Dawley rats (n=6) were fed either an AIN-76 diet (control, NDCon), control diet plus Acanthopanax senticosus extract (ND+Ex), a modified AIN-76 diet supplemented with 20% beef tallow (high fat, HFCon), or a high fat diet plus Acanthopanax senticosus extract (HF+Ex), for 5weeks. Body weight gain was significantly higher in the HFCon group than the NDCon group. Feed consumption was significantly lower, but energy intake higher, in the groups fed high fat diets compared with the groups fed control diets. Serum HDL-cholesterol concentrations were significantly increased but serum LDL-cholesterol concentrations were decreased in the groups fed the Acanthopanax senticosus extract. Abdominal fat accumulation, serum leptin levels were significantly higher in the HFCon group than the other groups. Carnitine palmitoyltransferase-I (CPT-I) mRNA levels were increased in the groups fed Acanthopanx senticosus extract. These results suggest that supplementation of cell cultured Acanthopanax senticosus extract regulates CPT-I mRNA levels in liver, has an effect on the normalization of lipids in rats fed a high fat diet.
Cha, Youn-Soo,Soh, Ju-Ryoun,Kim, Jae-Whune The Korean Society of Food Science and Nutrition 2003 Preventive Nutrition and Food Science Vol.8 No.1
Acanthopanax senticosus was grown by a novel, proprietary method, of culturing isolated cells in a bioreactor. An extract from the cells was evaluated for its effect on lipid metabolism in rats fed a high fat diet. Male Sprague-Dawley rats (n=6) were fed either an AIN-76 diet (control, NDCon), control diet plus Acanthopanax senticosus extract (ND+Ex), a modified AIN-76 diet supplemented with 20% beef tallow (high fat, HFCon), or a high fat diet plus Acanthopanax senticosus extract (HF+Ex), for 5weeks. Body weight gain was significantly higher in the HFCon group than the NDCon group. Feed consumption was significantly lower, but energy intake higher, in the groups fed high fat diets compared with the groups fed control diets. Serum HDL-cholesterol concentrations were significantly increased but serum LDL-cholesterol concentrations were decreased in the groups fed the Acanthopanax senticosus extract. Abdominal fat accumulation and serum leptin levels were significantly higher in the HFCon group than the other groups. Carnitine palmitoyltransferase-I (CPT-I) mRNA levels were increased in the groups fed Acanthopanx senticosus extract. These results suggest that supplementation of cell cultured Acanthopanax senticosus extract regulates CPT-I mRNA levels in liver and has an effect on the normalization of lipids in rats fed a high fat diet.
생물반응기 배양을 통한 두릅나무(Aralia elata)의 체세포배 및 유식물체 대량증식
이원석,최은경,김재훈,Lee, Won-Seok,Choi, Eun-Gyung,Kim, Jae-Whune 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.3
두릅나무의 엽병을 1.0 mg/L 2,4-D가 포함된 MS 고체배지에서 배발생캘러스를 유도하였다. 배발생세포와 배발생세포괴는 1.0 mg/L 2.4-D가 포함된 MS 액체배지에서 배발생캘러스를 2주간 현탁배양하여 대량으로 얻었다. 그물망을 통과한 배발생세포는 1.0 mg/L 2.4-D가 포함된 MS 액체배지에서 배양하면 배발생능이 소실되지 않고 지속적으로 유지 및 증식시킬 수 있었다. 그물망을 통과하지 못한 배발생세포괴를 식물생장조절물질이 첨가되지 않은 1/2 MS 액체배지에 옮겨 2주간 배양하면 구상형의 체세포배로 발달하였다. 구상형의 체세포배는 5 L의 bioreactor를 이용하여 배양하면 심장형, 어뢰형, 자엽형의 배와 유식물체로 발달하였다. Bioreactor 배양을 통해 두릅나무의 체세포배를 효과적으로 대량증식 시킬 수 있었다. Embryogenic calli were induced from petioles of Aralia elata on MS solid medium supplemented with 1.0 mg/L 2,4-D. When embryogenic calli were transferred to MS liquid medium supplemented with 1.0 mg/L 2,4-D, embryogenic cells and embryogenic cell clusters were developed after 2 weeks of culture. Embryogenic cells were filtered through a 250 ${\mu}{\textrm}{m}$ sieve and the passed cells were proliferated and maintained in MS liquid medium supplemented with 1.0 mg/L 2,4-D. Embryogenic cell clusters entrapped on the sieve were transferred to 1/2 MS liquid medium without plant growth regulators, globular-shaped embryos were developed from embryogenic cell clusters after 2 weeks of culture. Numerous early stage somatic embryos could be developed to heart-shaped, torpedo-shaped, cotyledonary embryos and plantlets in 5 L bioreactor. Above results suggest that effective somatic embryo proliferation can be achieved via bioreactor culture systems in Aralia elata.
Kang, Tae-Jin,Lee, Won-Seok,Choi, Eun-Gyung,Kim, Jae-Whune,Kim, Bang-Geul,Yang, Moon-Sik Elsevier 2006 Journal of biotechnology Vol.121 No.2
<P><B>Abstract</B></P><P>The B subunit of <I>Escherichia coli</I> heat-labile toxin (LTB) is a potent mucosal immunogen and immunoadjuvant for co-administered antigens. In order to produce large scale of LTB for the development of edible vaccine, we used transgenic somatic embryos of Siberian ginseng, which is known as medicinal plant. When transgenic somatic embryos were cultured in 130L air-lift type bioreactor, they were developed to mature somatic embryos through somatic embryogenesis and contained approximately 0.36% LTB of the total soluble protein. Enzyme-linked immunosorbent assay indicated that the somatic embryo-synthesized LTB protein bound specifically to G<SUB>M1</SUB>-ganglioside, suggesting the LTB subunits formed active pentamers. Therefore, the use of the bioreactor system for expression of LTB proteins in somatic embryos allows for continuous mass production in a short-term period.</P>