지상장애물 회피를 위한 항공기 경고 시스템에 관한 연구

함광근,최재덕,허웅 한국항공운항학회 1997 한국항공운항학회지 Vol.5 No.1

본 연구에서는 항공기의 지상활주에 필요한 측방 경보장치를 구현 하였다. 장치는 장애물 검출부, 송신부, 수신부, 그리고 경보부로 구성하였다. 장애물 검출부는 40KHz대의 초음파에 의한 펄스비행법을 채택하였고 송/수신부는 447 MHz 대의 RF모듈을 사용하였다. 경보부는 컴퓨터를 사용하여 수신된 거리 데이터로부터 경보를 한다. 검출장치는 주날개의 좌우측 끝과 수평 안정판의 좌/우측 끝에 장착한다. 장애물 검출거리는 10m로 설정하였다. 실험 결과 만족한 결과를 얻을 수 있었다. in this study, we deviced side warning system that is necessary to the ground operation of aircraft. The system consist of obstacle detection part, transmission part, receive part, and warning part. We used TOF(Time Of Flight) method using 4OkHz ultrasonic wave as the obstacle detection part. The 447MHz RF module was applied to the transmission and receive part. The warning part is activated by the computer using received distance data. The detection system attach to the left/right side edge of main wing and horizontal stabilizer. We have decided 10x11 obstacle detection range. The result of expriment was satisfaclory.

TLR4, 5, and 9 Agonists Inhibit Murine Airway Invariant Natural Killer T Cells in an IL-12-Dependent Manner

Jae-Uoong Shim,Joon Haeng Rhee,Young-Il Koh 대한천식알레르기학회 2012 Allergy, Asthma & Immunology Research Vol.4 No.5

Purpose: Invariant natural killer T (iNKT) cells may play an important role in the pathogenesis of asthma in mice and humans. Thus, an agent that modulates the function of iNKT cells may have therapeutic potential to control asthma. We hypothesized that lipopolysaccharide (LPS)-, flagellin-, or CpG-induced changes in the cytokine milieu may modify and even inhibit the function of airway iNKT cells in asthma. Methods: Because increased α-galactosylceramide (GalCer)-induced airway hyperreactivity (AHR) reflects the presence of airway iNKT cells, α-GalCer-induced AHR, as well as inflammatory cells and cytokines in bronchoalveolar lavage (BAL) fluid, were determined 24 hours after in vivo treatment with LPS, flagellin, or CpG in naïve BALB/c mice. Intracellular IL-4 and IFN-γ were measured in spleen iNKT cells after in vitro treatment with LPS, flagellin, or CpG. A role for IL-12 following the treatments was determined. Results: Intranasal administration of LPS, flagellin, or CpG reduced development of α-GalCer-induced AHR, eosinophilic airway inflammation, and Th1 and Th2 cytokine responses in BAL fluid, while producing IL-12 in BAL fluid. Intraperitoneal administration of IL-12 mAb blocked the suppressive effect of LPS, flagellin, or CpG. In vitro treatment with LPS, flagellin, or CpG reduced production of IL-4 and IFN-γ from α-GalCer-stimulated spleen iNKT cells; these effects were ameliorated by addition of anti-IL-12 mAb. Conclusions: TLR4, 5,and 9 agonists may suppress the function of airway and spleen iNKT cells via IL-12-dependent mechanisms. Anergy of iNKT cells by IL-12 might play a role in suppression by these TLR agonists.

Increased Th2-like Invariant Natural Killer T cells in Peripheral Blood From Patients With Asthma

Jae-Uoong Shim,고영일 대한천식알레르기학회 2014 Allergy, Asthma & Immunology Research Vol.6 No.5

Purpose: Invariant natural killer T (iNKT) cells might play an important role in asthma pathogenesis in humans. Our previous study found no differencein the number of blood iNKT cells between asthma patients and controls. However, few studies have examined the function of blood iNKTcells in human asthma. Methods: Twenty asthma patients and eight controls were included in this study. Blood iNKT cells were identified usingdouble staining with anti-Vα24 and anti-Vβ11 monoclonal antibodies (mAbs) or with 6B11 and anti-Vβ11 mAbs. Intracellular IL-4, IL-10, and IFN-γcytokines were stained in blood iNKT cells using their respective mAbs and isotypes. In addition, their relationships with clinical parameters wereanalyzed. Results: The number of Vα24+Vβ11+iNKT cells or 6B11+Vβ11+iNKT cells did not differ between asthma patients and controls. However,among Vα24+Vβ11+iNKT cells, the proportion of IL-4+iNKT cells was increased in asthma patients compared to controls (7.0±3.0% vs 0.5±0.4%,P<0.05). There were no differences in the proportions of IL-10+or IFN-γ+iNKT cells between the groups. The proportion of IL-4+cells among6B11+Vβ11+iNKT cells inversely correlated with FEV1, expressed as a percentage predicted value in asthma patients (Rs=-0.64, P<0.05, n=19). Conclusions: Blood iNKT cells are thought to be Th2-like, and IL-4-producing iNKT cells may be associated with lung function in human asthma.

뽕잎 당단백질의 항산화능과 Raw 264.7 세포에 있어서 bisphenol A에 유도된 신호전달인자의 억제

심재웅(Jae-Uoong Shim),이세중(Sei-Jung Lee),오필선(Phil-Sun Oh),임게택(Kye-Taek Lim) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.2

본 연구에서는 뽕잎 당단백질의 활성을 알아보기 위하여 뽕잎 당단백질의 안정성 및 특성을 알아보고, hydroxyl 라디칼, super-oxide anion 라디칼, DPPH 라디칼 등의 활성 산소종에 대한 항산화 효과들 살펴보았다. 또한 Raw 264.7 세포에 환경호르몬의 일종인 BPA와 뽕잎 당단백질(32kDa)을 함께 처리 하여, 뽕잎 당단백질의 활성산소증과 NO의 소거 능력뿐만 아니라 염증 매개성 단백질들 [NF-κB(p50)와 iNOS]의 활성 역제능력 대하여 평가하였다. 뽕잎 당단백질은 금속이온에는 다소 약하지만 온도와 pH에는 안정적인 특징을 지니고 있었으며, 탁월한 hydroxyl 라디칼, superoxide anion 라디칼, DPPH 라디칼 소거 능력을 가지고 있었다. 이러한 항산화 능력을 지닌 뽕잎 당단백질을 BFA와 함께 Raw 264.7 세포에 처리한 결과, BPA만 처리된 Raw 264.7 세포의 활성 산소종 양은 8시간째에, 그리고 NO 양은 24시간째에 현저히 증가한데 반해, BPA와 함께 뽕잎 당단백질을 처리한 Raw 264.7 세포에서는 같은 시간 동안에 농도에 비례하여 활성 산소종 및 NO양이 유의적으로 감소한 것을 알 수 있었다. 또한 8시간 동안 BPA저리에 의해 활성화된 Raw 264.7 세포의 NF-κB (p50)와 iNOS 단백질들은 함께 처리한 뽕잎 당단백질의 농도에 비례하여 현저히 억제되었다. 따라서, 이러한 결과를 종합해 보면, 뽕잎 당단백질은 높은 안정성과 강력한 항산화 효과를 가지고 있었으며, 이러한 항산화 효과가 환경 호르몬(BPA)에 의한 활성산소종 및 NO 생성을 저해한 뿐만 아니라, NF-κB(p50)와 iNOS의 활성을 억제함으로써 Raw 264.7 세포의 염증 신호전달을 막는데 영향을 끼쳤을 것으로 생각 된다. The present study investigated anti-oxidative and anti-inflammatory activity of glycoprotein isolated from Morus Indica Linne (MIL glycoprotein). We found that MIL glycoprotein has a molecular weight of 32 KD and consists of carbohydrate (40.03%) and protein (59.97%), and that it has a strong scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (OH), and superoxide anion (O₂ㆍ?) radicals. In addition, MIL glycoprotein had a stable character and an optimal DPPH radical scavenging activity in the alkaline and neutral pH solution, and up to at 105. However, the results indicated that it has a minimal scavenging activity in the metal ionic solution (Ca²?, Mn²?, and Mg²?) in the presence of EDTA. In addition, we further investigated whether MIL glycoprotein scavenges oxygen radicals and blocks inflammation-related signals in the bisphenol A (BPA)-stimulated Raw 264.7 cells. The results in this study showed that it has a character to scavenge the productions of reactive oxygen species (ROS) and nitric oxide (NO) dose-dependently. Also it blocked the activities of inflammation-related signals such as nuclear factor-kappa B (NF-κB) and inducible nitric oxide synthase (iNOS). For example, it had an inhibitory effect on the activation of NF-κB (p50) and iNOS proteins at 200 ㎍/㎖ MIL glycoprotein. Here, we speculate that MIL glycoprotein is one of natural antioxidants and of modulators of the BPA-induced inflammation.

Inhibitory Effect of Glycoprotein Isolated from Cudrania tricuspidata Bureau on Expression of Inflammation-Related Cytokine in Bisphenol A-Treated HMC-1 Cells

Shim, Jae-Uoong,Lim, Kye-Taek Springer-Verlag 2009 INFLAMMATION Vol.32 No.4

뽕잎 당단백질의 혈중지질 저하 효과 및 항산화 효과

심재웅(Jae-Uoong Shim),임계택(Kye-Taek Lim) 한국식품과학회 2008 한국식품과학회지 Vol.40 No.6

본 연구는 콜레스테롤 억제효과가 밝혀진 뽕잎에서 당단백질을 추출하여 과산화지질 라디칼 억제능력 및 생쥐의 혈장 콜레스테롤 수준과 간 해독효소 활성의 개선효과를 평가하였다. In vivo에서 생쥐에게 뽕잎 당단백질을 20 ㎎/㎏의 농도로 섭취시킨 후 콜레스테롤의 수치를 알아보고, 한편, Triton WR-1339를 투여한 생쥐 그룹에서 혈액 및 간 조직을 적출하여 혈장 콜레스테롤의 수준 변화 및 해독효소의 활성을 측정한 결과, 20 ㎎/㎏의 농도로 뽕잎 당단백질을 섭취시킨 생쥐그룹에서 총 콜레스테롤과 LDL 콜레스테롤의 수준이 급격히 감소하였고, HDL의 경우 증가하는 것을 확인할 수 있었다. 또한 Triton WR-1339를 투여한 고지혈증을 유도한 생쥐 그룹에서도 뽕잎 당단백질의 농도에 따른 총 콜레스테롤과 LDL 콜레스테롤의 유의적인 저하를 확인할 수 있었다. 간의 해독효소 중 항산화 기능을 가지고 있는 SOD, CAT 그리고 GPx의 활성은 뽕잎 당단백질의 섭취에 의해 모두 증가하였으나 특히 SOD, CAT의 활성이 크게 나타났고, 그에 비하여 GPx는 그 유의적 차를 느끼기 어려울 정도의 변화 뿐이었다. 일반적으로, 천연물로부터 추출한 당단백질은 항산화 능력을 가지고 있고, 또한 이런 항산화제는 동맥 혈관조직이나 백혈구를 손상시키는 체내에서 발생된 ROS를 제거하고 체내 혈장지단백질 수준에 있어서 반비례적인 상관관계가 있으며, 결과적으로 혈장 콜레스테롤 수준을 억제할 수 있다고 추론할 수 있다(4,25,26). 따라서 이러한 결과에 미루어볼 때, 뽕잎 당단백질 역시 간 내 해독효소의 활성을 증가시킴으로써 체내의 ROS 수준을 감소시키고, 콜레스테롤의 수준을 낮추었으므로 뽕잎 당단백질의 역할이 다른 천연물 유래의 당단백질과 마찬가지로 지단백질과 반비례적인 상관관계가 있는 항산화제 역할을 수행할 수 있다고 사료된다. 앞으로 HMG-CoA reductase에 의한 콜레스테롤 생성과 그게 관련된 유전자 발현 및 그 기전을 분자생화학적인 수준에서 보충적인 연구가 더 수행되어야 할 것이다. The objective of this study was to evaluate the effects of glycoprotein isolated from Morus indica L. (MIL) on plasma cholesterol levels and on the activities of hepatic detoxicant enzymes in ICR mice. MIL glycoprotein evidenced good scavenging activities against lipid peroxyl radicals. When the mice were treated with Triton WR-1339, the levels of total cholesterol (TC) and low-density lipoprotein (LDL)-cholesterol in plasma increased significantly by 53.9 and 47.5 ㎎/dL, respectively, as compared to the controls. However, when pretreated with MIL glycoprotein (100 ㎍/mL), ICR mice showed marked reductions to 55.4 and 47.0 ㎎/dL, as compared to Triton WR-1339 treatment alone. Interestingly, high density lipoprotein cholesterol levels were unchanged. These results indicate that the MIL glycoprotein is capable of scavenging lipidperoxyl radicals, lowering plasma lipid levels, and increasing the activities of detoxicant enzymes in the mouse liver.

Poster Ⅲ : Structure - Activity Relationship of Dynorphine B analogues

Jae Wook Lim,Nam Joo Hong,Chang Woo Lee,Yeung Tack Park,Tae Heung Song,Soon Uoong Koock 생화학분자생물학회(구 한국생화학분자생물학회) 1992 추계학술대회집 Vol.- No.-

Wilson씨병 10예에 대한 진단적 고찰

양현웅,김연수,문희석,박기오,이엄석,김선문,서승원,성재규,나병규,이승민,이병석,김남재,이헌영 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background/Aims : Wilson's disease in an autosomal recessive genetic disorder which is characterized by hepatic and neurologic manifestations due to defect in copper metabolism. The progression of hepatic damage and critical neurologic complication can be prevented by early diagnosis and treatment. Our study investigated the usefulness of clinical manifestations and laboratory findings in Wilson's disease to early diagnosis of wilson's disease. Method : We investigated the 10 patients who are diagnosed as Wilson's disease at Chungnam national university Hospital during the recent 5 years for clinical manifestation, laboratory findings and treatment. Results : 1. Females are 9 patients(90%) among 10 objective patients and the median age of patients is 30.2±14 years. The second and third decade are the more common prevalent and 5 patients(50%) have family history. 2. The clinical manifestations of Wilson's disease at time of diagnosis are hepatic manifestation in 9 patients (90%) except presymptomatic patient and the psycho-neurologic manifestations are appeared in 5 patients(50%). Three patients have the secondary amenorrhea, hemolytic anemia and recurrent spontaneous abortions in each case. 3. The serum ceruloplasmin concentrations were lower than 20mg/dl in 8 patient(80%) including presymptomatic patient and the 24 hours urine copper excretion is above 100μg/24hrs in 7 patients(70%). 4. Seven patients(70%) with symptomatic Wilson's disease have the detectable Kayser-Fleischer ring in slit lamp examinations but one presymptomatic patient has no Kayser-Fleischer ring. 5. The serum level of ceruloplasmin is increased in 3 patients (42.8%) among 7 patients who are had been treated by penicillamine(one patient is not followed up serum ceruloplasmin) and the 24 hours urine copper excretion was decreased in 6 patient(75%) among 8 patients treated by penicillamine. 6. Five patients died with hepatic failure related to uncompensated liver cirrhosis. Conclusion : The slit lamp examination should be recommended in patients with hepatic and neurologic disease of unknown etiology and sibling of the patients wilson's disease. The irrerersible damage of organs would be prevented by aggressive treatment at early state in patients diagnosed as Wilson's disease.

포스터 발표 : 대장 ; 대장암 수술후의 대장선종의 발생양상

나병규 ( Byung Kyu Na ),양현웅 ( Hyeon Uoong Yang ),서숭원 ( Soong Won Seo ),성재규 ( Sung Jae Kyu ),이병석 ( Byung Seok Lee ),정현용 ( Hyun Yong Jeong ) 대한소화기학회 2002 대한소화기학회 춘계학술대회 Vol.2002 No.-

〈목적〉 대장 직장암의 근치수술후 대장내시경 검사를 이용한 추적 검사를 통해 대장 선종의 발생 양상을 분석함으로써 대장직장암 환자에서의 대장선종 발생의 특징을 알아보고자 하였다. 〈방법〉 1998년 3월부터 2001년 2월가지 충남대학교병원에서 대장직장암으로 근치적 절제술을 시행 받은 환자중 수술전가 수술후에 대장내시경을 받았던 130명의 환자를 대상으로 의무기록을 통한 후향적 조사를 하였다. 〈결과〉 남자는 80명, 여자는 50명이었고 평균 연령은 5

생표고버섯 전신 알레르기 접촉피부염

조규만 ( Kyu Man Cho ),심재웅 ( Jae Uoong Shim ),유지은 ( Ji Eun Yu ),고영일 ( Young Il Koh ) 대한천식알레르기학회(구 대한알레르기학회) 2015 Allergy Asthma & Respiratory Disease Vol.3 No.6

It has been reported that ingestion of raw or undercooked shiitake mushrooms is associated with various adverse food reactions. A 58-year-oldman presented with pruritic and maculopapular rashes on the trunk and extremities starting 1 day after ingestion of partially uncooked shiitake mushrooms. A probable diagnosis of systemic allergic contact dermatitis was made. Raw and cooked shiitake mushroom-derived allergen extracts were prepared, and patch and intradermal tests with delayed readings were performed. The case showed positive intradermal test results with delayed readings to the raw, but not cooked, extracts. The case suggests that ingestion of uncooked shiitake mushrooms may induce systemic allergic contact dermatitis through type IV hypersensitivity reaction. (Allergy Asthma Respir Dis 2015;3:452-455)