Agrobacterium tumefaciens KU12 로부터 분리한 pTi12 의 Replication Origin 과 벼의 Actin 유전자 프로모터를 이용한 벼의 Binary Vector 제조

박희등(Hee Deung Park),박정구(Jeong Ku Park),이재선(Jae Seon Lee),심웅섭(Woong Seop Sim) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.4

Binary vectors, pBI-ActR1, pBI-ActF1 and pBSH-ActR1, were constructed using pGA642, the replication origin of pTil2 and the rice actin promoter. The sizes of pBI-ActR1, pBI-ActF1 and pBSH-ActR1 were 12.9 kb, 13.2kb and 11.95 kb, respectively. These vectors containing a rice actin promoter followed by a GUS structural gene could induce strongly the expression of GUS gene in transformed rice cells. Rice explants form 3-4 day old seedlings after germination were cocultured with A. tumefaceins harboring pBI-ActR1, pBI-ActF1 or pBSH-ActR1, and then GUS expression in the explants was assayed. Transformation of rice explants by these binary vectors was tissue-specific, such that the meristematic regions of shoot apex, root and hypocotyl were transformed by these binary vectors.

다양한 rhBMP-2 코팅 임플란트 표면에서의 인간 간엽 줄기세포의 부착, 증식, 분화반응 분석

이인구,한인호,황선욱,류재준 대한치과보철학회 2012 대한치과보철학회지 Vol.50 No.1

연구 목적: 본 실험은 거친 표면으로 유의성 있는 줄기세포반응을 나타냈던 4 가지의 티타늄 표면 위에 rhBMP-2를 코팅했을 때 어떤 유의한 줄기세포반응(세포부착, 증식, 분화)이 나타나는지 비교 분석함으로 rhBMP-2 코팅을 위한 가장 적절한 표면을 평가하기 위해 시행되었다. 연구 재료 및 방법: 대조군인 기계절삭표면(machined surface)과 실험군인 양극산화(anodized), RBM, SLA 표면에 rhBMP-2를 코팅한 후 코팅하지 않은 표면과 같이 8가지 표면 위에 인간줄기세포를 배양하였다. 배양 후 24시간 후SEM을 통해 줄기세포의 부착을 평가하였고 배양 3, 7, 14일후MTT와 ALP 검사를 통해 줄기세포의 증식과 분화반응을 평가하였다. 그리고 배양 7일후RT-PCR 검사를 통해 Type I collagen, osteocalcin, osteopontin의 유전자 발현의 변화를 평가하였다. 결과:SEM 평가에서 4가지 rhBMP-2 표면이 코팅하지 않은 표면에 비해 세포부착 면적이 넓고 긴밀하며 세포돌기가 더 많이 관찰되었다. 양극산화 rhBMP-2코팅표면에서 가장 두드러지게 관찰되었다. MTT 검사에서 크게 의미 있는 차이는 나타나지 않았다. ALP검사에서 양극산화 rhBMP-2코팅 표면은 대조군과 비교해서 (3, 14일) 또 RBM rhBMP-2 코팅 표면과 비교해서 (14일) 유의성 있는 ALP 활성도의 증가를 나타내었다(P<.05). RT-PCR 검사에서 osteocalcin과 osteopontin의 유전자 발현은 양극산화 rhBMP-2코팅 표면에서 높게 나타났다. 결론: 양극산화 rhBMP-2코팅표면이 줄기세포의 부착과 분화실험에서 대조군표면과 rhBMP-2를 코팅한 기계절삭표면이나 RBM 표면에 비해 유의성 있는 증가를 나타냈다(P<.05). PURPOSE. In this paper we tried to evaluate the most appropriate surface for rhBMP-2 coating among 4 rough titanium surfaces. MATERIALS AND METHODS. We used machined surface as a control group and anodized, RBM and SLA surfaces as test groups. We coated rhBMP-2 on the 4 surfaces and with uncoated surfaces for each case, we cultured human mesenchymal stem cells on all 8 surfaces. 24 hours after we measured the stem cell’attachment with SEM, and on 3rd, 7th, and 14th days, we checked the cell proliferation and differentiation by using MTT and ALP activity assay. And on the 7th day after the culture, we performed RT-PCR assay to determine whether the expression levels of Type I collagen, osteocalcin, osteopontin were changed. RESULTS. We observed with SEM that 4 rhBMP-2 coated surfaces exhibited wider and tighter cell attachment and more cell process spreading than uncoated surfaces. The anodized rhBMP-2 surface caused robustest effects. In MTT assay we could not find any meaningful difference. In ALP assay there was a significant increase (P<.05) in the ALP activity of anodized rhBMP-2 coated surface compared with that of the control (3rd and 14th days) and with that of the RBM rhBMP-2 coated surface (14th day). In RT-PCR assay there was increased expressions in the anodized rhBMP-2 coated surface for osteocalcin, and osteopontin. CONCLUSION. We found that the anodized rhBMP-2 coated surface were most prominent stem cell attachment and differentiation in compared to control and Machined rhBMP-2 coated, RBM rhBMP-2 coated surface.

烏貝散이 HCI-aspirin 으로 유발된 십이지장궤양에 미치는 조직학적 영향

구세광,이형식,김종대,한상원,김상찬,이재현 慶山大學校 基礎科學硏究所 1999 基礎科學 Vol.3 No.-

오패산이 HCI-aspirin tm으로 유발된 rat 십이지장궤양에 미치는 조직학적 영향을 관찰하기 위하여, 오패산을 1,3 및 5일 동안 경구투여하고 십이지장의 조직학적 변화를 관찰하였던 바 다음과 같은 결과를 얻었다. 실험 전 기간동안 대조군에서는 십이지장 융모의 위축과 붕괴가 관찰되었으며, 조직학적으로는 점막상피의 변성 및 탈락이 관찰되었고 점막고유층에서는 염증세포의 침윤(infilteration)의 소견이 관찰된 반면 오패산 투여군의 투여 1일에서는 대조군과 유사하게 관찰되었으나, 투여 3일후부터 점막상피의 첨부에서 경미한 카타르성 물질이 관찰된 이외에 정상군과 유사한 소견을 나타내었다. 이상에서 오패산이 십이지장궤양의 치유를 촉진시키는 것으로 관찰되었다. In order to study, the histological effects of Opeaesan on the duodenal ulcer induced by HCI-aspirin tm in rate, experiments were done by oral administration and observed histological profiles of the ulcerative lesions. Ulcerative lesions were unmerously detected in control groups especially in pylorus-duodenal junctions. Histologically, very severe injury to duodenal epithelium wer observed in control groups and these injuries were increased with time intervals. But in the Opaesan-administration groups, no gross lesion of ulcer were demonstrated and histologically minor injury to the mucosal epithelium were observed. Among the Opaesan treatment groups, most slight injuries to duodenal mucosa were detected in 5 days after administration when the histological profiles were similar to that of normal groups. According to these result, it is suggested that Opaesan should be used in treatment of the duodenal ulcer but the funcitonal mechanisms were unknown.

대두유와 소금의 첨가에 따른 동부 묵의 기계적 텍스쳐 및 관능적 특성

구미영,이재경,김기숙 한국조리과학회 2002 한국식품조리과학회지 Vol.18 No.3

This study was carried out to investigate the effects of soybean oil and salt on the mechanical texture and sensory characteristics of Dongbu-Mook (Cowpea Starch Gel). As the amount of the soybean oil was increased, the hardness and chewiness of the Mook were decreased. But there were no significant difference in the cohesiveness and springiness. While the Mook samples made with commercial Dongbu-Mook flour didn't show a significant difference, those made with lab-manufactured Dongbu-Mook flour without salt showed higher hardness, chewiness than the ones with salt. The Mook made of commercial Dongbu-Mook flour was found to be higher in the hardness and lower in the cohesiveness than the one made of lab-manufactured Dongbu-Mook flour. In sensory evaluation, as the amount of soybean oil was increased, all the samples showed higher yellowness and greasiness, and lower clarity, hardness and chewiness. For the samples with salt, the yellowness was high, and the hardness was low. The Mook samples with lab-manufactured Dongbu-Mook flour had low chewiness. There was no significant difference in the clarity and greasiness. Overall, the Mook samples made with commercial Dongbu-Mook flour showed the best quality, when soybean oil was not added and salt was added. For the Mook made with lab-manufactured Dongbu-Mook flour, the sample with 2% oil and salt was the best.

만성 신부전 환자에서 혈액투석에 의한 Isoniazid의 제거

신재국,차인준,장인진,신상구,김윤구,한진석,김성권 大韓臨床藥理學會 1994 臨床藥理學會誌 Vol.2 No.2

고춧가루의 증자와 이를 이용한 김치의 품질

구연수,조재욱,김순동 대구효성가톨릭대학교 식품과학연구소 1997 식품과학지 Vol.9 No.-

김치담금재료 중 미생물이 많이 오염되어 있는 고춧가루를 증자법으로 살균하였을때의 문제점을 조사하여 보완함과 아울러 이 방법으로 처리하여 얻은 고춧가루를 이용하여 김치를 담구었을때의 품질변화를 조사하였다. 고춧가루를 증자법으로 살균한 결과, 120℃에서 10분이상 증자하면 총균수와 대장균은 검출되지 않았으나 고춧가루의 색상이 검게 변하므로 증자용수의 pH를 달리하여 증자한 결과 pH 10으로 조정된 증자수로 10분간 증자하였을때가 L^(*), C^(*)값이 높았으며 Hab˚는 10분까지는 거의 변화가 없어 무처리와의 색상차가 거의 없었다. pH 10으로 조정한 증자용액으로 10분간 증자한 고춧가루로 담근 김치의 pH는 발효전반에 걸쳐서 대조구보다 높은 pH를 보였다. 색상은 증자처리구가 대조구에 비해 L^(*), Hab˚값이 낮고 C^(*)가 높아서 숙성전기간동안 밝은 붉은색을 띠었다. 미생물수는 증자처리구가 전 발효기간을 통해 총균수와 젖산균수가 다소 적었다. 신맛은 대조구는 발효 10일째에 보통이상 신맛을 보였으나 증자처리구는 발효 15일째에도 보통이상의 신맛을 보였다. 매운맛은 대조구와 증자처리구의 뚜렷한 차이가 없었으며 숙성전기간동안 보통이하의 값을 나타내었다. 종합적인 맛과 육안적으로 본 품질은 숙성전기간 동안 대조구에 비해 증자처리구가 높게 나타났다. In order to enhance the shelf-life and quality of kimchi, kimchi was fermented with steamed red pepper powder. And the quality was evaluated by sensory test and measurement of microflora, color, pH and titratable acidity. Total cell count and E. coli of red pepper powder steamed at 120℃ for over 10minutes were not detected. But the color of red pepper powder was turned into darkness. So, steamed red pepper powder by various pH of steaming water showed effective pH and steaming time were 1 and 10minutes, respectively. Thus, pH of kimchi made by steamed red pepper powder for 10minutes, using steaming water adjusted pH 10, was gradually decreased after 10 days of fermentation. The L^(*) and Hab˚values of the kimchi with steamed red pepper powder were higher than control products during overall fermentation but C^(*) value was lower. The number of microbe of kimchi using steamed red pepper powder was decreased that of control products. Sensory test of the control kimchi revealed a strong sour taste after 10days of fermentation. In comparison, the kimchi with steamed red pepper powder revealed a sour taste only after 15days of fermentation. The overall taste of kimchi with steamed red pepper powder showed better than that of control kimchi throughout the fermentation periods. This experiments showed that the quality of kimchi made by steamed red pepper powder can the best at 15th day of fermentation.

각종 殺菌劑가 主要果樹의 花粉發芽 및 花粉管伸長에 미치는 影響

具滋馨,李載昌 충남대학교 농업과학연구소 1978 農業技術硏究報告 Vol.5 No.1

The experiment was carried out in order to choose fungicides which are proper to be used in flowering time of fruit trees. The effect of fungicides on pollen germination and pollen tube growth was investigated in vitro. The results obtained are as follows : 1. Difolatan-Wp, Lime-sulfur, Captan-Wp and Bordeaux mixture greatly inhibited pollen germination and pollen tube growth. 2. The effect of Daconil-Wp and Zimaneb-Wp on pollen germination and pollen tube growth was not so great as that of Difolatan-Wp, Line-sulfur, Captan-Wp or Bordeaux mixture. 3. Polyram-combi-Wp, Topsin-Wp and Benlate-Wp did not affect pollen germination but these fungicides slightly inhibited pollen tube growth. 4. Polyoxin-Wp significantly increased pollen tube growth but did not show promotive effect on pollen germination.

하수관로 연결 돌출부 절단기 유닛 개발

김재열,김영석,안재신,곽이구,송경석,이창선 한국공작기계학회 2003 한국공작기계학회 춘계학술대회논문집 Vol.2003 No.-

The issue with the drainpipe now a day is that they are laid underground. Causing us to perform additional work to repair, such as digging up the ground and peeling off the insulator that surrounds the pipe. And such series works are difficult that concession appears from government and municipal office. However, if we can save time and money. Performance of piping robot that we are studied in existing session through fixing unit and improvement of cutting byte shorten and wished to heighten work efficiency. This is why we are trying to develop a unit that can cut up the projecting parts which connects with the interior part of the drainpipes.

탄수화물에 관한 연구 : 디아세톤글루코스의 헥소퓨라노스고리에 대한 산화와 입체선택적 변형에 관한 연구 Oxidation and Stereoselective Modification of the Hexofuranose ring Derived from Diacetone Glucose

강석구,조현성,이재호 성균관대학교 기초과학연구소 1989 論文集 Vol.40 No.2

천연물 합성에서 출발물질로 유용하게 쓰이는 디아세톤 글루코스 헥소퓨라노스고리의 산화와 입체선택적 변형에 관하여 연구하였다. 이 고리의 C-3위치에 있는 히드록시기를 Trifluoroacetic anhydride(TFAA)로 사용하여 케토기로 산화시키는 방법을 연구한 후 얻어진 C-3 위치에 케톤에 대해 Grignard반응과 Wittig반응을 도입하여 퓨라노스고리의 C-3위치에 입체 선택적으로 치환기를 유도하는 방법을 연구하였다. 또한 고리밖의 곁가지의 변형도 아울러 연구하였다. We have studied on the oxidation and the stereoselective modifications of the hexofuranose ring derived from diacetone glucose. We have attempted the method of the oxidation with trifluoroacetic anhydride (TFAA) on the C-3 position of this furanose ring. On the other hand, we have induced the stereoselective substrate on the keto group of the C-3 position. The modifications of the side chain out of the ring have been also studied.

傷害와 診斷書

河在鳩 大韓法醫學會 1989 대한법의학회지 Vol.13 No.2

One of the most important reports compiled by a physician is not required by statute. It is the medical report concerning the injuries, physical and mental condition, and progmosis of a patient-client, prepared by the physician in connection with a personal injury lawsuit or any other litigation involving the health and well-being of one of the parties. In this article, therefore, discussed conception of injury and infliction of injury, name of injury and essential parts for examination of the injuries, duration of treatment and criteria of determination of wound healing, etc.