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전해산성수를 이용한 새로운 내시경 소독 시스템(Cleantop WM-1??)의 소독효율
이준행,이풍렬,송재훈,이남용,임윤정,장재권,김영호,김재준,백승운,이종철,최규완 대한감염학회 2001 감염 Vol.33 No.2
Background : Electrolyzed acid water (EAW) is a new liquid chemical germicide containing a mixture of oxidizing species and prepared by mixing a small amount of salt with tap water in an electrolyzer. We attempted an in-use evaluation of the high-level disinfection capability of Cleantop WM-1□, a new endoscope reprocessing system using EAW. Methods : Forty-seven flexible endoscopes were randomly collected just after upper endoscopic examinations and were disinfected using Cleantop WM-1□. Parts of the endoscopes without contact to EAW were disinfected with 75% ethyl alcohol. Immediately after the disinfection procedure, samples were taken from the biopsy channel (S-1), tip of the insertion tube (S-2), the umbilical cord (S-3), and the angulation knob (S-4). Results : In EAW-disinfected parts of the endoscopes, the culture-positive rates were 4.3% (2/47) in S-1 samples and 12.8% (6/47) in S-2 samples. In ethyl alcohol-disinfected area, the culture-positive rates were 2.1% (1/47) in S-3 samples and 25.5% (12/47) in S-4 samples. The colony counts of culture-positive samples ranged from 1 to 144. Pseudomonas aerugi-nasa was recovered from the angulation knob of an endoscope, but other contaminating organisms were mostly : normal flora or opportunistic pathogens. Conclusion : Although part of the endoscopes disinfected with 75% ethyl alcohol were contaminated with more organisms than EAW-disinfected areas, Cleantop WM-1□ showed a relatively good disinfection efficacy in reprocessing patient-used endoscopes. (Korean J Infect Dis 33:133∼138, 2001)
이행남,박길문,모양우,이덕구,설재림,김건일 朝鮮大學校 機械技術硏究所 2004 機械技術硏究 Vol.7 No.2
The ejector is used to get low pressure, and it has been applied to a lot of industry field like the heat engine, the fluid instrument power plant, the food industry, environment industry etc. because there are not any problem even it is mixed with kinds of liquid, gas, and solid. The flow characteristics in diffuser are investigated by PIV and CFD. The agreement between numerical analysis and experiment shows the validity of this study and the results of this study would be useful to the engineers who design for the flow systems for heating, ventilation, air conditioning and wastewater purification plants.
시토크롬 C의 전기화학반응에 있어서 프로모터와 전해질과의 영향
최광재,조홍식,이행자,장상목,김영한 동아대학교 환경문제연구소 1999 硏究報告 Vol.22 No.1
The electrochemical reaction of cytochrome C varied with promotors and electrolytes. Among electron transfer carriers, 4-4' bipyridine(PyPy) showed good redox response using 20 mM phosphate solution as electrolyte and the case of Bis(4 pyridyl) bisulphide(PySSPy) as promotor showed same result using 0.2M Sodium cacodylate as electrolyte tested cyclic voltammetry method. Between electrodes, Au electrode is better behaviour than ITO electrode. 0.21M Tris, 0.24M Cacodylate acid showed the most effective electrochemical characteristics as electrolyte.
내분비계 교란물질의 검출계를 이용한 γ-HCH의 미생물에 의한 중간대사산물에 대한 내분비계교란 활성의 평가
이행석,박주석,조은민,문명숙,太田明德,류재천 대한상하수도학회 2003 상하수도학회지 Vol.17 No.1
To develop an efficient degradation system for Endocrine disruptors (EDs), it is necessary to have a good system to evaluate rapidly and accurately endocrine-disrupting activities of suspected chemicals and their degradation products. We previously constructed a co-expression system of GAL4 DNA binding domain (DBD)-human estrogen receptorβ ligand binding domain (hERβ LBD) and Gal4p transcriptional activation domain (TAD)-co-activator SRC1 in Saccharomyces cerevisiae with a chromosome-integrated lacZ reporter gene under the control of CYC1 promoter and GAL4 binding site (Upstream Activating Sequence, UAS). Expression of this reporter gene was dependent on the presence of estrogen or endocrine disruptors in the culture medium. Furthermore, the extent of transcriptional activation by those chemicals correlated with their estrogenic activities measured by other assay systems, indicating that this assay system is efficient and reliable for measuring estrogenic activity. We applied this assay system to measure estrogenic activity of microbial degradation products of γ-hexachlorocyclohexane (γ-HCH) by Sphingomonas paucimobilis. Among the γ-HCH metabolites, 2,5-dichlorohydroquinone (2,5-DCHQ) and chlorohydroquinone (CHQ) had similar estrogenic activities to the original chemical, but hydroquinone (HQ), a metabolite at later stage, had no activity at the concentration of 10^-4M, showing the necessity of evaluation of intermediate metabolites in microbial degradation systems.
에스트로겐 투여가 난소절제로 유도된 백서의 골다공증에 미치는 효과에 관한 실험적 연구
허균행,김경욱,이재훈 단국대학교 치의학연구소 1994 논문집 Vol.6 No.2
Osteoporosis is a state of reduced bone mass per unit volume with a normal ratio of mineral to matrix. There were many methods for osteoporosis, and estrogen is one of them. In this study, 30 female rats were ovariectomized to make a state of estrogen deficiency and induce osteoporosis. After 13 weeks, 15 ovariectomized rats were injected 6 times with 60㎍/㎏ of estradiol by 1 week interval (experimental group). The remaining 15 ovariectomized rats were used as control group. According to the time schedule, both of them were sacrified and femur were harvested. Paraffin sections were stained with HematoxylinEosin staining and modified Gomori's Trichrome staining, and observed under light microscope. The results were as follows : 1. There were no significant bony changes between the experimental and control group of cancellous bone. 2. The density of cortical bone showed increasing with time in experimental group. 3. The resorption of cortical bone in experimental group was less than that of control group.
생약추출물 유도형 Nitric Oxide Synthase 저해활성 검색
류재하,이소영,박재현,이화진,장세란,은진희,김남이,정연수,장미경,최영은,이숙현,손행자,안한나,고혜진 숙명여자대학교 약학연구소 2001 약학논문집-숙명여자대학교 Vol.17 No.-
Nitric Oxide (NO), derived from L-arginine, is produced by two types (constitutive and inducible) of nitric oxide synthase (NOS: cNOS and iNOS). The NO produced in large amounts by the iNOS is known to be responsible for the vasodilation and hypotension observed in septic shock and inflammation. The inhibitors of iNOS, thus, may be useful candidate for the treatment of inflammatory diseases accompanied by the overproduction of NO. We prepared alcoholic extracts of herbal drugs which have been used for the treatment of inflammation in oriental medicine. We have screened the inhibitory activity of NO production in lipopolysaccharide (LPS)-activated macrophages after the treatment of these extracts. Among the 81 kinds of extracts of herbal drugs, 34 extracts showed potent inhibitory activity of NO production above 50% at the concentration of 50 (μg/ml. The inhibitory activities of NO production were also evaluated for several solvent fractions at three different concentrations. Especially, hexane soluble fractions of Agrimonia pilosa, Hydrangea serrata, Machilus thunbergii, Prunella vulgaris, Saussurea lappa, Tussilago farfara, and ethyl acetate soluble fractions of Angelica gigas, Ostericum koreanum, Torilis japonica, Perilla frutescence showed moderate activity at 10 and/ or 25 (μg/ml. These fractions are promising candidates for the study of the activity-guided chromatographic purification of active compounds.
입상활성탄에 의한 BTX 및 활로겐화알칸의 기상흡착특성
이행자,정영언,최광재,손진언 東亞大學校 大學院 1993 大學院論文集 Vol.18 No.-
Gas phase adorption characteristics of BTX and halogenated alknaes vapor onto granular activated carbon in the fixed bed were investigated by experimentally. The following results were obtained. 1. For vapor of GAC-BTX and GAC-halogenated alkanes systems, uptake curves were obtained at 298K at various concentration range. 2. Capacity of adsorption of solvents vapors onto granular activated carbon was in the order as follows: ① BTX: m-Xylene> toluene> Benzene> 0-Xylen ②. Halogenated alkanes : CHCl₃>CCl₄>CH₂CL₂ 3. Kangmuir coefficient of each components were shown the TAble 3. 4. RElation of amount adworbated versus surface diffusivity coefficient was obtained like equation D?=a + bq and coefficient a and b were shown the Table 7.
최행숙,김은경,백성혜,이길재,정완호 한국교원대학교 과학교육연구소 2001 청람과학교육연구논총 Vol.11 No.1
In this study, the contents of elementary school science textbooks related to 'heat and temperature' are analyzed and alternative conceptions of "heat and temperature" among students enrolled in grades 4, 5 and 6 are investigated. 259 students were selected from a elementary school located in urban area. The relationships between students' alternative conceptions and the content of elementary science textbooks are also examined. Students' conceptions are analyzed from their answers to a paper-and-pencil test. The typical alternative conception of "heat and temperature" held by students was follows. They think that "heat" is a material and "temperature" is value of heat amount. They can't distinguish between "heat" and "temperature". Their explanation of "heat and temperature" is focused on hot or cool sensation and other observable characteristics of a material. A textbook analysis indicated that contents on "heat and temperature" were organized without the viewpoint of particle motion theory. This may be one of the causes of students' alternative conceptions.
이형재,김용두,정현숙 順天大學校 師範大學 附屬 科學敎育硏究所 1994 科學과 敎育 Vol.2 No.-
미나리는 독특한 풍미 및 색깔을 지니고 질감과 영양이 뛰어나서 그 이용이 증가되고 있으나, 아직 연구가 미흡한 점을 주시하여 보다 나은 미나리의 활용을 위한 과학적 기초 자료를 얻고자, 미나리의 성분 특성 및 열처리와 냉각방법을 달리하여 ascorbic acid와 시각적 기호도에 영향을 미치는 chlorophyll의 변화를 검토하였는데 그 결과를 요약하면 다음과 같다. 무기성분은 칼륨과 칼슘의 함량이 높았고 줄기보다 잎에서의 함량이 높아 약 1.0-3.4 배의 차이를 나타냈다. 총 아미노산 함량은 줄기와 잎에서 각각 2139.50mg%, 3031.27mg% 였으며 잎의 함량이 줄기보다 1.42배 높았고 총 아미노산에 대한 필수 아미노산의 비율은 줄기가 37.14%, 잎이 37.71%로 나타났다. 확인된 아미노산은 16종이었으며 각 아미노산의 함량은 줄기에서 aspartic acid와 arginine이 주요 구성 아미노산으로 전체의 22.08%를 차지하였고 lysine > histidine > glutamic acid 순으로 함량이 많았으며 잎의 경우는 glutamic acid, arginine이 주요 구성 아미노산으로 18.93%를 나타냈고, aspartic acid > leucine> lysine의 순으로 함유되었으며, 이는 줄기의 주요 아미노산의 함량과 차이를 나타냈다. 줄기와 잎에서 ascorbic acid 함량은 각각 7.60mg%, 8.72mg%였으며 cholrophyll의 함량은 각각 16.33mg/g, 34.83mg/g로 잎부위가 줄기부위보다 높았다. 열처리 방법에 따라서 ascorbic acid 보유율은 microwave 열처리에서 가장 높았고 1000㎖식염수(1%) > 1000㎖ >500㎖ 순이었으며, chlorophyll 보유율은 1000㎖ 식염수 (1%) >microwave > 1000㎖ > 500㎖ 순으로 높았다. 냉각 방법에서도 실온 방치 냉각보다 냉수 냉각이 훨씬 더 높은 ascorbic acid, chlorophyll 보유율을 보였다. The use of the waterdropwort(Oenanthe javanica DC) as both health food and taste food tends to increase, while the researches on it have not been sufficiently made. For the purpose of obtaining the scientific, basic data for the better use of the water dropwort, the present thesis deals with the researches on the changes in the water dropwort : the ascorbic acid and chlorophyll change depending upon the varied heating and cooling methods which influence visual taste. The research results are as follows. The water dropwort contained greater amount of minerals such as K and Ca, and the leaf had 1.0-3.4 times as much content as the stem. The total amino acid content was 2139.50 mg% in the leaf -1.42 times more in the leaf than in the stem. The ratio of essential amino acid to the total amino acid was 37.14% in the stem and 37.71% in the leaf. 16 different kinds of amino acid were detected from the water dropwort. Aspartic acid and arginine were the major amino in the stem, representing 22.08% of the total, and the content was in the order of lysine, histidine, glutamic acid. Glutamic acid and arginine were the major amino acid in the leaf, representing 18.93% of the total, and the content was in the order of aspartic acid, leucine, lysine. The content of ascorbic acid was 7.60 mg% in the stem and 8.72 mg% in the leaf. The content of chlorophyll was 16.33 mg/g in the stem and 34.83 mg/g in the leaf. When the water dropwort was heated, the retention rate of ascorbic acid was the highest in the case of microwave heating method, and the rate was high in the order of 1000㎖(1% NaCl)>1000㎖>500㎖. The retention rate of chlorophyll was high in the order of 1000㎖(1%NaCl)>microwave>1000㎖>500㎖. When the water dropwort was cooled, the retention rate of ascorbic acid and chlorophyll was higher in the cool water cooling than in the room temperature cooling.