제주도 숲섬 파초일엽(Asplenium antiquum Makino)의 보존 현황에 관한 조사연구

김주환,김윤식 대전대학교 이과대학 기초과학연구소 1997 自然科學 Vol.- No.-

1996년 7월부터 1997년 5월까지 현지 방문조사와 인근 지역의 탐문조사를 수행하여 천연기념물 제18호로 지정된 제주도 서귀포 숲섬의 파초일엽 보존상태와 현황을 조사하였다. 조사 결과, 제주도 숲섬 내 파초일엽은 이전의 박만규, 이영노의 보고와 마찬가지로 자연생육지에서 절멸된 것으로 생각되며, 이후 여러 차례의 복원 노력이 시도되었던 것을 확인하였다. 그러나, 현재의 이식복원품의 기원에 대해서는 생육지가 확실한 일본, 대만 등 여러 지역의 식물체를 대상으로 한 분자유전학적 연구와 같은 보다 면밀한 연구가 요구되며, 향후 연구 결과에 따라 본 지역의 체계적이고도 과학적인 보호대책의 수립이 절실하다. This study was conducted to elucidate the conservation state and circumstances of Asplenium antiquum Makino, the Natural Memorial Product no. 18 at Sup-som, Seokyoi-po in Cheju Island Field surveys and visiting in-vestigations were performed three times from July 1996 to May 1997. On the result, it k supposed that natural living plants from Supsom were disappeard likewise previous reports by Park, M.K(1975) and Lee, Y.N.(1990). Also, there were several efforts to recover the living place and transplant Asplenkrm anriquum Makino. But, it is needed that the further study to find out the origin of present transplanted plants through the investigation of wide areas comparing with the plants from Taiwan, Japan and etc. and molecular genetic study such as RAPD and AFLP in detail. Also, the systematic and scientific protection plan is needed according to the results of futher study.

GT rotary file을 이용한 만곡 근관형성시 작업 효율 및 file 변형 발생에 관한 연구

신주희,백승호,배광식,임성삼,윤수한,김병현 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.5

Root canal preparation process is of utmost importance in successful treatment of root canal. Also, one of the most important purpose of the root canal preparation is to enlarge the root canal three dimensionally without changing the curvature of the root canal. However, as the curvature of the root canal increases, there are many difficulties involved in formation of optimum root canal. Therefore, in order to solve the above mentioned problems, new developments in methods of root canal preparation and equipments for such purposes were made. Recently, vigorous studies about newly introduced engine-driven nitckel-ti-tanium rotary file are conducted. As shown in research results to dates, it is well established that the use of nickel-titanium file is better suited for curved root canal than stainless steel file in maintaining the curvature of root canal and reducing the deformation of root canal. However, it is also acknowledged that there are a few discrepancies in research results according to protocol, due to failure to remove variables in experiments. In addition, although it is recommended by the manufacturer that the GT rotary file should maintain a low rotational speed of 150∼350rpm and 'light pressure' as light as not to break the lead of a pencil, academic studies about the vertical force which is not yet standardized are not sufficiently explored. Therefore, this research devised and utilized a special research equipment to standardize the appropriate range of vertical force for GT rotary file through experiments by breaking of the lead of a pencil as expressed by the manufacturer and to accurately measure factors involved through repeating and recreating the environment of root canal preparation. Forming nine experimental groups by varying the vertical forces (150g, 220g, 300g) and rpm (150rpm, 250rpm, 350rpm), the effects of changing vertical forces and rpm on working efficiency were measured in terms of time expended in root canal preparation by crown-down method using a transparent resin block with 35 degree curvature and GT rotary file (z-test). The following research using this special research equipment that involved nine experimental groups and varying the vertical force for root canal preparation from 300g which is within the normal vertical force range to 700g and 1000g which fall outside the normal rpm range. The results were as follows : 1.Analysis of the experiment results revealed that the tome spent in root canal preparation decreased as the vertical forces and rpm increased (p<0.05). Also, the effects of rpm were greater than those of the vertical forces within the normal vertical force range (β-weight test) . 2.Observation of the deformation of GT rotary file revealed that deformation increases in a direct correlation with the vertical force increase and in a reverse correlation with the rpm decrease, In the case of the vertical forces close to the normal range, the probability of GT rotary file deformation were quite different depending on the rpm changes. In the case of◎eater tier◎cal forces, the occurrences of deformation of the file were more frequent regardless of the rpm changes. 3.Deformation and breakage of file were also commonly observed in the expended time measurement experiments and GT rotary file deformation experiments in which low speed rpm (150rpm) was used and at the curved portion of the resin block. Key words : GT rotary file, Crown-down method, Working efficiency, Vertical force, Rpm, Deformation and breakage of file

관상동맥 질환에서 CD14 유전자형에 따른 세균 및 Heat Shock Protein에 대한 반응의 차이

한주용,최수연,조현주,김화평,강현재,구본권,김남중,김효수,손대원,오병희,박영배,최윤식 대한감염학회 2007 감염과 화학요법 Vol.39 No.1

Background : CD14 is the receptor for lipopolysaccharides and heat shock protein (HSP), which has been suggested being associated with increased risk of coronary artery disease (CAD). We investigated whether the response to infectious agents or HSP is different according to CD14 polymorphism in Koreans. Materials and Methods : Antibody titers to Helicobacter pylori, Chlamydia pneumoniae, and human HSP60 (hHSP60) were measured in 48 patients with stable CAD and in 41 healthy controls by ELISA. CD14 genotype was determined by PCR and high-sensitivity C-reactive protein (hs-CRP) was measured. Results : Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly associated with the presence of CAD. CD14 genotype distribution was 31 TT (35%), 43 CT (48%), and 15 CC (17%). To compare the response to the infectious organism and hHSP60, we divided study population into 3 groups; CAD patients with non-TT genotype (group I, n=30), CAD patients with TT genotype (group II, n=18), and normal controls (group III, n=41). Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly different among 3 groups. Though hs-CRP level was significantly different among 3 groups, post-Hoc analysis showed that hs-CRP level was not significantly different between group I and group II (group I: 1.6[1.1-3.5] mg/L and group II: 0.35[0.1-2.0] mg/L). Conclusions : This study suggests that the inflammatory responses to infectious organisms and HSP do not differ according to the CD14 genotype in Koreans. 목적 : 만성적인 세균 감염이나 자가 면역 반응이 동맥경화와 연관되어 있다는 주장이 제기되어 왔다. CD14은 lipopolysaccharides (LPS)와 heat shock protein(HSP)의 수용체로 C(-260)→T 다형성이 관상동맥 질환의 위험과 관련되어 있다고 제안되어 왔다. 본 연구에서는 한국인에서 CD14 다형성에 따른 LPS와 HSP에 대한 반응성을 염증표지자를 측정하여 살펴보고자 하였다. 재료 및 방법 : 안정형 관상동맥 환자 48명과 정상 대조군 41명을 대상으로 Chlamydia pneumoniae, Helicobacter pylori 항체 및 인체 HSP60 (hHSP60) 항체 역가를 enzyme-linked immunosorbent assay (ELISA) 방법으로 측정하였다. CD14 유전자형은 중합효소연쇄반응을 이용하여 결정하였고 hs-CRP를 측정하였다. 결과 : C. pneumonias 및 H. pylori 항체 양성 여부 및 항체 역가, 그리고 hHSP60에 대한 항체 역가는 안정형 관상동맥 질환의 유무와 유의한 상관관계는 없었다. CD14 유전자형의 분포는 TT 30명(39%), CT 31명(40%), 그리고 CC 16명(21%)이었다. 관상동맥 질환 군에서 TT 유전자형은 38%, 대조군에서는 32%로 TT 유전자형과 관상동맥 질환의 유무와는 유의한 상관관계가 발견되지 않았다(P>0.05). CD14 유전자형에 따라 C. pneumonias와 H.pylori 감염 및 hHSP60에 따른 반응에 차이가 있는지 여 부를 알아보기 위해, 관상동맥 질환 군을 다시 TT 유전자형 군과 non-TT 유전자형 군으로 나누어, 전체적으로 세군에서 항체 양성률과 역가를 비교하였다(I군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, II군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, III군: 정상 대조군). 세 군에서 C. pneumonias와 H. pylori 항체 양성률 및 hHSP60 항체 역가에는 유의한 차이가 없었다. 세 군사이에 hs-CRP 값에 유의한 차이가 있었지만, post-Hoc분석에서 II군의 hs-CRP 값이 I군에 비해 유의하게 높지는 않았다(0.35 [0.1-2.0] mg/L in group II vs. 1.6 [1.1-3.5]mg/L in group I, P>0.05). 결론 : 본 연구는 한국인에서 CD14 유전자형에 따라 LPS나 HSP에 대한 반응이 유의하게 다르지 않음을 시사한다.

言語學과 外國語 敎育

鄭周鎬,李正植 서원대학교 교육연구소 1986 敎育發展 Vol.5 No.1

The method of foreign language teaching has been developing according to linguistics. Instead of the grammar- translation method based on prescriptive grammar, the audio - lingual approach appeared along with structural grammar. Reflecting the idea of stimulus → reaction relation and empiricism on language acquisition, it recommended to teach speaking and hearing prior to grammar and translation. It doubtlessly contributed much to the teaching of living language, while it depended on the mechanical drill of fixed sentence patterns too much, thus causing the originality of learners to be checked. Reflecting the idea of rationalism on language acquisition according to transformational grammar, the cognitive code theory put emphasis on the cultivation of the active originality as well as the linguistic competence of learners, by teaching them sentence structures through transformation drill. But the linguistic competence of transformational grammar is criticized as restrictive by the communicative approach based on socio - linguistics. Considering the socio - cultural and psycho - linguistic relation, the communicative approach insists that it is important to heighten the learners' will to learn by teaching them contextually appropriate language rather than grammatical sentence structures. Those methods above criticize each other, but they should rather complement each other, I think. The repetition drill of audio- lingual approach should be extended to the voluntary command of the language, and the linguistic competence of cognitive code theory should be supplemented to teach the appropriate language to the given situation. A foreign language, thus, should be taught as a natural language, harmonizing linguistic performance, linguistic competence and contextual appropriacy.

응집 체계에 관한 이론적 고찰

김주식 동아대학교 인문과학대학 영어영문학과 1991 동아영어영문학 Vol.7 No.-

Bacillus sp.의 조효소 xylanase, β-xylosidase가 칡넝쿨(pueraria thunbergiana) 섬유의 분해에 미치는 영향

이주식,정채인 Natural Science Research Institute JEONJU UNIVERSI 1990 전주대학교 자연과학연구소 학술논문집 Vol.3 No.1

In order to microbiologically degrade the xylan compound exist in epidermis of the plant Pueraria thunbergiana, four Bacillus spp. exerting xylolytic activity have been isloated from Pueraria thunbergiana. The optimal and stable conditions of pH and temperature for the activities of crude xylanase and β-xylosidase produced by isolates have been determined. One strain, named JUP-4, out of four isolates showed the best activity. The optimal pH for activity of both crude enzymes of JUP-4 was 9.0. The optimal temperatures of xylanase and β-xylosidase were 60℃ and 50℃, respectively. It was resulted from this study that the isolate JUP-4 was a good candidate for the biodegrating agent of epidermis of Pueraria thunbergiana.

Praziquantel이 肺吸蟲의 幼若成蟲에 미치는 영향에 관한 電子顯微鏡的 硏究

尹疇植,金洙鎭,林漢鍾 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.1

This study was undertaken to observe the morphological changes of young adult worms(45th day after infection) of Paragonimus westermani obtained from experimentally infected dogs after treatment with praziquantel. For this study 8 dogs were artificially infected with metacercariae isolated from crayfish, Cambaroides similes. After 45th and 90th day of infection, three dogs were each given praziquantel 3×25 mg/kg×1day respectively, and autopsied at 24 hours after the last medication. With the young adult worms from the cysts in the lungs of treated dogs, the fine structural changes were observed by means of scanning and transmission electron-microscope. The findings were compared with those of adult worms in treated and untreated control host. The results are as follows: 1. The majority of the young adult worms obtained from the treated dogs showed a large balloon-shape structure on the tegumental surface between oral and ventral suckers. This structural change has never been reported in the adult worms obtained from the treated hosts. 2. The scanning electron-microscopy revealed formations of bleb-like structures on sensory papillae near the oral and ventral suckers. 3. The transmission electron-microscopy showed that the axons of the nerve endings in the sensory papillae around two suckers were degenerated and formed large vesicles which upon rupturing on the tegumental surface have formed bleb-like structures. 4. The numerous mitochondriae in the tegumental syncytium of the worms in treated hosts appeared to be degenerated and formed small vacuoles, and a different size of vacuoles interspersed in the distal part of the tegumental syncytium. These vacuoles were fused to each other and led to the disruption of the apical region of tegumental syncytium along the basement layer, which was finally detached from the circular muscles of the worm. 5. Many host cells (granulocytes) invaded and destroyed the degenerated tegument by the occurrence of blebs or the exposed muscular layer of the worm after exfoliation of the tegument.

한국산 화살나무속(노박덩굴과)의 수리분류학적 연구

김주환,최병희,김윤식 대전대학교 이과대학 기초과학연구소 1997 自然科學 Vol.- No.-

한국산 화살나무속 16분류군의 분류군간의 한계를 규명하고 속내분류체계의 타당성을 논하고자, 화살나무속 107개의 OTU를 대상으로 60개의 외부형태형질에 대한 유집분석과 주성분분석의 수리분류학적 연구를 수행하였다. 중심연결법과 Ward방법에 의한 두 가지 유집분석의 결과, 유집군들의 구성이 거의 동일하게 나타났으며, grouprks에는 유사도가 매우 높은 것으로 관찰되었고, 각 종간에는 독립적으로 높은 수준의 유집이 형성되어 뚜렷한 불연속성이 존재하는 것으로 나타났다. 주성분분석결과 주성분1(38.4%), 주성분2(27.5%), 주성분3(17.2%)이 총 83.2%의 기여율을 보였으며, 이를 3차원공간에 투영하여 뚜렷한 5개의 유집군으로 구분할 수 있었다. 본 연구결과, 속내 각 종집단간의 명확한 불연속성이 관찰되었으나, sect. Biloculares의 분류학적 위치에 관한 문제점등이 제시되었다. 또한, 속내 분류체계에 있어서 Blakelock의 견해가 타당한 것으로 판단된다. This study was conducted to elucidate the delimitations of 16 taxa of Euonymus in Korea and to discuss the infrageneric classification system among the treated taxa by cluster analysis and principle components analysis (PCA) using 107 OTUs with 60 morphological characters. Based on the results of cluster analyses by the Ward and the UPGMC methods, there were similarities on the composition of clustered taxa and distinct discontinousity independently among the species. The treated taxa were divided into five groups by the PC1, PC2 and PC3, and the sums of contributions for the total variance were 83.2% (PCI 38.4%, PC2 27.5% and PC3 17.2%, respectively). This study suggested that there were taxonomic problems on the limits and composition of taxa in sect. Biloculares. The current study supported the infrageneric system of Euonymus proposed by Blakelock.

寶城郡 熊峙面에 있어서의 肺吸蟲症의 疫學的 推移

李駿商,裵平元,林漢鍾,申鴻植 한국농촌보건회 1980 농촌의학·지역보건 Vol.5 No.1

흉부자상에 의한 심실중격결손 및 승모판 부전증 치험 1예

김범식,조순걸,곽영태,조규석,박주철,유세영 慶熙大學校 1985 論文集 Vol.14 No.-

A 34-year-old man was stabbed with knife in the left fourth intercostal space. He was admitted to Kyung Hee University Hospital Emergency room with Cardiac tamponade state. Through a left anterior thoracotomy, a tense hemopercardium was released. A 2㎝ wound of right ventricle was sutured with 3-0 prolein. Seven month after the injury he was readmitted for cardiac catheterization. Cardiac catheterization confirmed the presence of an interventricular septal defect in the membranous septum with left ventricle to right ventricle shunt. A laceration of the septal leflet of mitral valve was also demonstrated. The pulmonary blood flow as 1.22 times the systemic flow. A longitudinal incision was made on the right atrium on the cardiopulmonary bypass. An 1×0.5㎝ interventricular septal defect was found in the membranous septum. In order to achieve and adequate repair interventricular spetal defect and mitral insufficency. The ventricular septal defect was repaired 3-0 prolein plegeted suture with interrupted. Artifical atrial septal defect was made and the septal leflet defect(1.5㎝) was reattached with 3-0 prolein plegeted suture interrupted. The partient had and uneventful post operative recovery and was discharged the Hospital on the 24day post operative day.