Imazethapyr 유도체의 제초활성에 미치는 3-(N-methyl-N-(X)-치환-phenylaminooxoacetyl) group의 영향

성낙도,김현재,장해성,김대황 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

새로운 25종의 Imazethapyr 유도체, (2-(4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl)-3-(N-methyl-N-(X)치환 -phenylaminooxoacetyl)-5-methylpyridine)들을 합성하여 치환기(X) 변화에 따른 발아 전 후, 피(Echinochla crus-galli.)의 제초활성에 미치는 3-(N-methyl-N-(X)치환 -phenylaminoozoacetyl) group의 영향을 검토한 바, 발아 전보다 발아 후의 제초활성에 더 큰 영향을 미침을 알 수 있었다. 발아 후의 제초활성은 X-치환기의 전자밀게 효과와 입체상수(E_s)에 의존적이었으며 가장 큰 제초활성을 나타내는 화합물로는 bulky(E_s<O)하고 전자밀게 (б<O)가 치환된 화합물, 15(4-t-butyl group)와 20(3,5-dimethyl group)이었다. 그리고 높은 제초활성을 나타낼 것으로 예상되는 화합물의 조건들이 검토되었다. (1993년 9월 18일 접수, 1993년 9월 22일 수리). New twenty five Imazethapyr derivatives, [2-(4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl)-3-(N-methyl-N-X(sub.)-phenylaminooxoacetyl)-5-methylpyridine] were synthesized. and The quantitative structure activity relationships (QSARs) between their post-emergence herbicidal activity(pI_50) values in vivo against Barnyard grass (Echinochloa crus-galli) and physicochemical parameters of substituents(X) of 3-(N-methyl-N-X(sub.)-phenylaminoo-xoacetyl) group have been studied. From the basis on the findings, in case of post-emergence, the activities were dependent on the steric constant(E_s<θ)and electron donating (o<O) effect by subsitituents(X) of 3-(N-methyl-N-X(sub.)phenylaminooxacetyl) group. Therefore, The most effective compound, 15 (4-t-butyl group) and 20 (3,5-dimethyl group) were examined in this study. And the conditions on the compounds predicted to show higher herbicidal activity were also discussed.

비점곡선으로부터 기액평형의 결정

임진남,곽철 한국화학공학회 1981 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.19 No.3

9가지의 2成分系(n-hexane∼n-butanol)系, acetone∼methanol系, acetone∼n-propanol系, methanol∼n-propanol系, n-hexane∼n-propanol系, acetone∼n-hexane系, methanol∼n-butanol系, methanol∼n-hexane系, acetone∼n-butanol系)에 대하여 760mmHg에서 沸點을 實測했다. 여기서 얻은 沸點데이터를 기초로 하여 各 系에 대한 成分 1과 成分 2의 無限稀釋活動度係數를 구했다. 또한 Wilson 式을 기초로 하여 無限稀釋活動度係數로부터 Wilson parameter와 氣液平衡値를 구하였으며 計算値와 文獻値 및 實驗値와 比較하였다. Boiling points of 9 binary systems(n-hexane-n-butanol, acetone-methanol, acetone-n-propanol, methanol-n-propanol, n-hexane-n-propanol, acetone-n-hexane, methanol-n-butanol, methanol-n-hexane, acetone-n-butanol) were measured at the 760mmHg. Using the Gautreaux & Coates equation. infinite activity coefficients of each system were calculated from the boiling poins curves of the 9 systems. Furthermore from the infinite activity coefficients Wilson parameters and isobaric vapor-liquid equilibria data were obtained. These data were compared with the experimental and reported data.

Effects of Type of Oilseed and Level of Concentrate on Fermentation, Biohydrogenation of Fatty Acids and Conjugated Linoleic Acid Production in a Rumen-Simulated Continuous Culture System

Choi, N. J. 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.4

본 연구는 사료 내 농후사료의 비율을 높임으로서 불포화 지방산의 반추위 내 by-pass율의 향상 가능성을 조사하고, 아마종실과 전지대두 급여 시 CLA 생산을 상호 비교하기 위하여 연속배양장치를 이용하여 수행하였다. 지방 급원에 따른 발효 성상의 차이는 보이지 않았다. 한편 사료 내 농후사료 비율이 높은 (80%) 처리구는 농후사료 비율이 낮은 (40%) 처리구와 비교하여 pH는 감소하였으나, 암모니아, 총 휘발성 지방산, acetate, butyrate 및 valerate 농도가 증가되었다. 지방급원 (전지대두 vs 아마종실)과 사료 내 농후사료 비율은 organic matter(OM), total nitrogen, neutral detergent fiber(NDF) 및 acid detergent fiber (ADF)의 소화율에 영향을 끼치지 않았다. 반면에 전지대두는 아마종실과 비교하여 trans C18:1, C18:2 n-6 및 C18:3 n-3 유출율은 증가시켰다. 지방 급원에 의한 CLA flow는 영향을 받지 않았으나 사료내 농후사료 비율이 높을 때와 전지대두와 아마종실의 함량이 높았을 때는 증가되었다. 수소 첨가현상은 C18:1 n-9 와 C18:2 n-6에서 지방 급원에 의하여 영향을 받지 않았으나, 아마종실 처리구에서는 C18:3 n-3 과 총 C18 불포화지방산의 수소 첨가현상이 전지대두 처리구와 비교하여 높은 비율로 발생했다. 한편 사료내 농후사료 비율이 높을 때 처리구에서 C18:2 n-6, C18:3 n-3 및 총 C18 불포화 지방산의 수소 첨가현상은 농후사료 저 처리구와 비교하여 감소되었다. This experiment employed a rumen simulated continuous culture system to examine the possibility of improving the rumen bypass of polyunsaturated fatty acids (PUFA) by using a high proportion of concentrate in feed, and compared soya an linseed in terms of conjugated linoleic acid (CLA) production. NO effect of type of fat source was observed on ruminal fermentation. A high proportion of concentrate (80%) in the feed decreased (P<0.001) vessel ? but increased (P<0.01) ammonia nitrogen, total VFA, acetate, butyrate and valerate concentrations compared with a low proportion (40%). Fat sources (soya vs. linseed) and concentrate ratio in the feed did not affect digestibilities of organic matter (OM), total nitrogen, neutral detergent fiber (NDF) and acid detergent fiber (ADF). Soya incerased the flows of trans C18:1, C18:2 n-6 and C18:3 n-3 compared with linseed. The difference in fat source alone did not affect the flow of CLA but this was increased when high levels of soya and linseed were associated with a high proportion of concentrate in the fee. There was no effect of fat source on biohydrogenation of C18:1 n-9 and C18:2 n-6, but biohydrogenation of C18:3 n-3 and total C18 PUFA was higher with the linseed than with the soya treatment. A high proportion of concentrate decreased biohydrogenation of C18:2 n-6, C18:3 n-3 and total C18 PUFA compared with a low proportion.

Carbon tetrachloride - Cyclohexane -Benzene 계 및 Ethanol - n - Propanol - n - Heptane 계의 혼합열

임진남,곽철 한국화학공학회 1982 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.20 No.1

Carbon tetrachloride-cyclohexane, carbon tetrachloride-benzene, cyclohexane-benzene, ethanol-n-propanol, ethanol-n-heptane 그리고 n-propanol-n-heptane계에 대하여 2성분계 혼합열을 25.0℃에서 측정하였고 carbon tetrachloride-cyclohexane-benzene계 및 ethanol-n-propanol-n-heptane계의 3성분계 혼합열을 25.0℃에서 측정하였으며 2성분계 데이터에서 구한 정수로부터 3성분계 혼합열을 추산하는 방법을 제시하였고 추산치를 실험치와 기왕의 문헌치와 비교하였다. The heats of mixing for the binary systems of carton tetrachloride-cyclohexane, carbon tetrachloride-benzene, cyclohexane-benzene, ethanol-n-propanol, ethanol-n-heptane, and n-propanol-n-heptane were measured at 25.0℃. The heats of mixing for the ternary systems of carbon tetrachloride-cyclohexane-benzene and ethanol-n-propanol-n-heptane were also measured at 25.0℃. The heats of mixing for the ternary systems were estimated from the results of the corresponding binary systems and the predicted values were found to be in good agreement with experimental and literature data.

Diabetes augments cognitive dysfunction in chronic cerebral hypoperfusion by increasing neuronal cell death: Implication of cilostazol for diabetes mellitus-induced dementia

Kwon, K.J.,Lee, E.J.,Kim, M.K.,Kim, S.Y.,Kim, J.N.,Kim, J.O.,Kim, H.J.,Kim, H.Y.,Han, J.S.,Shin, C.Y.,Han, S.H. Blackwell Science ; Academic Press 2015 Neurobiology of disease Vol.73 No.-

Many patients with diabetes are at increased risk of cognitive dysfunction and dementia. Diabetes mellitus is a vascular risk factor that may increase the risk of dementia through its associations with vascular dementia. We tested whether cognitive impairment could be exacerbated in combined injury using a rat model of chronic cerebral hypoperfusion with diabetes. We also determined whether a potent inhibitor of type III phosphodiesterase could prevent the cognitive decline caused by this combined injury. We used Otsuka Long-Evans Tokushima Fatty (OLETF) rats as a model of type II diabetes (T2DM) and Long-Evans Tokushima Otsuka (LETO) rats as a control. Chronic cerebral hypoperfusion was modeled by permanent bilateral common carotid artery occlusion (BCCAO). At 24weeks, the non-diabetic and T2DM rats were randomly assigned into groups for the following experiments: analysis I (1) sham non-diabetic rats (n=8); (2) hypoperfused non-diabetic rats (n=9); (3) sham T2DM rats (n=8); (4) hypoperfused T2DM rats (n=9); analysis II- (1) sham T2DM rats without treatment (n=8); (2) cilostazol-treated T2DM rats (n=8); (3) hypoperfused T2DM rats (n=9); and (4) hypoperfused T2DM rats and cilostazol treatment (n=9). The rats were orally administered cilostazol (50mg/kg) or vehicle once a day for 2weeks after 24weeks. Rats performed Morris water maze tasks, and neuronal cell death and neuroinflammation were investigated via Western blots and histological investigation. Spatial memory impairment was exacerbated synergistically in the hypoperfused T2DM group compared with the hypoperfused non-diabetic group and sham T2DBM group (P<0.05). Compared with the control group, neuronal cell death was increased in the hippocampus of the hypoperfused T2DM group. Cilostazol, a PDE-3 inhibitor, improved the memory impairments through inhibition of neuronal cell death, activation of CREB phosphorylation and BDNF expression in the hypoperfused T2DM group. Our experimental results support the hypothesis that there are deleterious interactions between chronic cerebral hypoperfusion and T2DM. That is, metabolic diseases such as diabetes may exacerbate cognitive impairment in a rat model of vascular dementia. We also suggest that surprisingly, the phosphodiesterase III inhibitor, cilostazol may be useful for the treatment of cognitive impairment in diabetes mellitus-induced dementia. In conclusion, diabetes can aggravate cognitive dysfunction in vascular dementia, and PDE-3 inhibitors, such as cilostazol, may form the basis of a novel therapeutic strategy for diabetes-associated cognitive impairment or vascular dementia.

Neutron-Induced Activation Cross Sections on Hafnium Isotopes from the Threshold to 20 MeV

V. Semkova,N. Janeva,N. Koyumdjieva,R. Jaime Tornin,A. Moens,A. J. M. Plompen,K. Volev 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.23

Results of new cross section measurements are presented for the following neutron-induced reactions: ^(178)Hf(n,α)^(175)Yb, ^(180)Hf(n,α)^(177)Yb, ^(177)Hf(n,p)^(177)Lu^g, ^(178)Hf(n,x)^(177)Lu^g, ^(180)Hf(n,p)^(180)Lu, ^(180)Hf(n,n'α)^(180)Hf^m, ^(174)Hf(n,2n) ^(173)Hf, ^(176)Hf(n,2n)^(175)Hf, and ^(177)Hf(n,3n)^(175)Hf obtained with the activation technique. The irradiations were carried out at the 7-MV Van de Graaff accelerator at IRMM, Geel. Quasi monoenergetic neutrons with energies between 14.8 and 20.5 MeV were produced via the ^3H(d,n)^4He reaction at E_d = 1, 1.4, 2, 3, and 4 MeV. The ^3H(p,n)^3He reaction was employed for the production of neutrons in the 2 - 3 MeV energy range. Both natural and enriched samples were used to facilitate correction for interference between reactions leading to the same product. The radioactivity of the samples was determined by standard γ-ray spectrometry using HPGe detector. The current measurements are compared with the data from other authors and Evaluated Nuclear Data Files. Cross sections for three of the studied reactions are reported for the first time.

쥐의 근육 및 간장 콜레스테롤 수준에 미치는 사료 중 n-3 / n-6 지방산 , PUFA / SFA 및 MUFA / SFA 비율의 반응표면 분석

박병성(B . S . Park),고병대(B . D . Ko),이영철(Y . C . Rhee),황보종(J . Howang - Bo),성기승(K . S . Seong),이남형(N . H . Lee) 한국영양사료학회 1995 韓國營養飼料學會誌 Vol.19 No.5

Impact of low dose atorvastatin on development of new-onset diabetes mellitus in Asian population: Three-year clinical outcomes

Park, J.Y.,Rha, S.W.,Choi, B.,Choi, J.W.,Ryu, S.K.,Kim, S.,Noh, Y.K.,Choi, S.Y.,Akkala, R.G.,Li, H.,Ali, J.,Xu, S.,Ngow, H.A.,Lee, J.J.,Lee, G.N.,Kim, J.,Lee, S.,Na, J.O.,Choi, C.U.,Lim, H.E.,Kim, J.W Elsevier/North-Holland Biomedical Press 2015 INTERNATIONAL JOURNAL OF CARDIOLOGY Vol.184 No.-

Background: High dose atorvastatin is known to be associated with new onset diabetes mellitus (NODM) in patients with high risk for developing diabetes mellitus (DM). However, low dose atorvastatin is more commonly used as compared with high dose atorvastatin. The aim of this study is to investigate the impact of low dose atorvastatin (LDA, 10mg or 20mg) on the development of NODM up to three years in Asian patients. Methods: From January 2004 to September 2009, we investigated a total of 3566 patients who did not have DM. To adjust for potential confounders, a propensity score matching (PSM) analysis was performed using the logistic regression model. After PSM (C-statistics: 0.851), a total of 818 patients (LDA group, n=409 patients and control group, n=409 patients) were enrolled for analysis. Results: Before PSM, the cumulative incidence of NODM (5.8% vs. 2.1%, p<0.001), myocardial infarction (0.5% vs. 0.1%, p-value=0.007), and major adverse cardio-cerebral event (MACCE, 1.8% vs. 0.7%, p-value=0.012) at three-years were higher in the LAD group. However, after PSM, there was a trend toward higher incidence of NODM (5.9% vs. 3.2%, p=0.064) in the LDA group, but the incidence of MACCE (1.2% vs. 1.5%, p-value=1.000) was similar between the two groups. In multivariable analysis, the LDA administration was tended to be an independent predictor of NODM (OR: 1.99, 95% CI: 1.00-3.98, p-value 0.050). Conclusions: In this study, the use of LDA tended to be a risk factor for NODM in Asian patients and reduced clinical events similar to the control group. However, large-scale randomized controlled trials will be needed to get the final conclusion.

Direct analysis of site-specific N-glycopeptides of serological proteins in dried blood spot samples

Choi, N. Y.,Hwang, H.,Ji, E. S.,Park, G. W.,Lee, J. Y.,Lee, H. K.,Kim, J. Y.,Yoo, J. S. Springer Science + Business Media 2017 Analytical and Bioanalytical Chemistry Vol.409 No.21

<P>Dried blood spot (DBS) samples have a number of advantages, especially with respect to ease of collection, transportation, and storage and to reduce biohazard risk. N-glycosylation is a major post-translational modification of proteins in human blood that is related to a variety of biological functions, including metastasis, cell-cell interactions, inflammation, and immunization. Here, we directly analyzed tryptic N-glycopeptides from glycoproteins in DBS samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS) without centrifugation of blood samples, depletion of major proteins, desalting of tryptic peptides, and enrichment of N-glycopeptides. Using this simple method, we identified a total of 41 site-specific N-glycopeptides from 16 glycoproteins in the DBS samples, from immunoglobulin gamma 1 (IgG-1, 10 mg/mL) down to complement component C7 (50 mu g/mL). Of these, 32 N-glycopeptides from 14 glycoproteins were consistently quantified over 180 days stored at room temperature. The major abundant glycoproteins in the DBS samples were IgG-1 and IgG-2, which contain nine asialo-fucosylated complex types of 16 different N-glycopeptide isoforms. Sialo-non-fucosylated complex types were primarily detected in the other glycoproteins such as alpha-1-acid glycoprotein 1, 2, alpha-1-antitypsin, alpha-2-macroglobulin, haptoglobin, hemopexin, Ig alpha 1, 2 chain C region, kininogen-1, prothrombin, and serotransferrin. We first report the characterization of site-specific N-glycoproteins in DBS samples by LC-MS/MS with minimal sample preparation.</P>

Imazethapyr 유도체의 제초활성에 미치는 3 - ( N - methyl - N - ( X ) - 치환 - phenylaminooxoacetyl ) group 의 영향

성낙도(N . D . Sung),김현재(H . J . Kim),장해성(H . S . Chang),김대황(D . W . Kim) 한국응용생명화학회 1993 Applied Biological Chemistry (Appl Biol Chem) Vol.36 No.5

New twenty five Imazethapyr derivatives, [2-(4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl)-3-(N-methyl-N-X(sub.)-phenylaminooxoacetyl)-5-methylpyridine] were synthesized. and The quantitative structure activity relationships (QSARs) between their post-emergence herbicidal activity(pI_(50)) values in vivo against Barnyard grass (Echinochloa crus-galli) and physicochemical parameters of substituents(X) of 3-(N-methyl-N-X(sub.)-phenylaminooxoacetyl) group have been studied. From the basis on the findings, in case of post-emergence, the activities were dependent on the steric constant(E_s$lt;0) and electron donating (σ$lt;0) effect by subsitituents(X) of 3-(N-methyl-N-X(sub.)phenylaminooxoacetyl) group. Therefore, The most effective compound, 15 (4-t-butyl group) and 20 (3,5-dimethyl group) were examined in this study. And the conditions on the compounds predicted to show higher herbicidal activity were also discussed.