結核補體結合性抗原 관한 硏究

徐仁銖 漢陽大學校 1979 論文集 Vol.13 No.-

The author has observed the specificity and antigenecity of two kinds of antigen, one of which is carbohydrate moiety and the other is the protein. Measurement of hemolysin titre: The immunized serum of rabbits by sheep RBC was used as haemolysin, and it was used in three fold concentration of the complete hemolysis titrated preliminary test. Hemolysin was always used after deciding its titre at preliminary tet. The sheep RBC suspension was used by 3 per cent. The dosage of antigen:1/2 amount of the maximum haemolysin does, determined by the result of preliminary test of the author's two kinds of antigen, was used. Complement: Complement was obtained by the heart puncture of healthy guinea pigs. The two fold amount of the complement titre at preliminary titration was used in this experiment. Test sera were all supplied from the from the patients who were recieving treat-ment in the T. B. centre attached to the Ministry of Health and Welfare of Korea. These sera were inactivated by heating at 56℃ for 30 min., and when they were needed preservation, they were stored in refrigerator at 40℃± and were reheated at 56℃ for 15min. just before submitting to the experiment. Procedures of making antigens: C antigen-Very virulent human tubercle bacillus strain C is cultivated for 40 days on Sauton's non-protein media, and washed several times with saline on the filter papers. After dessication, the organisms are suspended in saline at the rate of 10 mg/ml and submitted to the rapid manipulation of freezing and thawing between -20℃ and 20℃;after 20 times of this manipulation, it is centrifuged at 8,000 rpm for 30 min, and its supernatant is picked up as C antigen. P-antigen: The same dried strain as used in making the C antigen is heated by steam at 100℃ for 30min., filtered through Seitz filter, into this filtered fluid added 40% trichlor acetic acid at the rate of 4 per cent, and set this fluid at 4℃±all day long and overnight. After then, its sedimented substance is isolated, suspended in 1% trichlor acetic acid solution, centrifuged by 3,000 rpm for 10 min., and its sedimented substance is re-picked up. This procedures are repeated twice more quite the same way, and into the finally got substance .by centrifugation is added 0.3% KH₂PO₄in approximate amount, and, after enough mixing, it is centrifuged so as to wash away the trichlor acetic acid. This manipulation of washing must be repeated three timesm and then the sediment is dried without heating, its powder is dissolved in phosphate buffered solution (pH 7.0) at the rate of 1.0 mg/ml, then it is the stock solution of P antigen, (the dried powder was got at the rate of 10.1mg from 2,500 ml of filtered fluid, and the total nitrogen amount was 13.34% by microki jeldahl method). The complement-fixation-reaction was carried out in T. B. serum using P-and C-antigens made by the author, and following results were obtained: 1) Both P-and C-antigens had neither anti-complement, nor exclusive hemolytic action. 2) Antigenecity of C-antigen is weak, and becomes weaker by heating and by preserving long time, while that of P-antigen being considerable high and never influenced by heating or by long period of preservation that is, not decreased even by heating at 60℃ for 30minutes, nor by preservation for 3 days at 37℃, nor for one month at room temperature. 3) Proving of antibody for the complement-fixation-reaction in lung T.B. serums is difficult immediately after drawing of blood, but it gradually increases as time goes, about 7 days later it becoming the maximum, and ,then after 12 days, again, it disappears. 4) Antibody detecting rate of lung T.B. is paralell with the degree of disease severity, but in utmost severe cases it's detection is rather difficult. 5) The rate of non-specific positive reaction by P-antigen with syphilis serum is low. 6) But with leprosy serum the non-specific positive rate of P-antigen is considerably high, especially in nodular case it is twice that of nervous leprosy. 7) The non-specific positive rate of P-antigen to the serum of healthy person is very low.

우리나라 손해보험회사의 지급능력확보에 관한 小考 : 지급여력제도를 중심으로

이수호,조기인 韓國海洋大學校 人文社會科學大學 1999 韓國海洋大學校 人文社會科學論叢 Vol.- No.7

The purpose of this study is to evaluate the solvency margin and its application along with the possibility of introducing RBC(Risk-Based Capital). The regulation of financial states of non-life insurance companies of korea comprises management evaluation and solvency margin. The solvency margin regulation of Korea is established based on the European Model. It requires a company should retain the net asset exceeding the possibility of bankuptcy; which is calculated that the sum of premium and solvency margine is under the sum insured and operation expenses. The European Model has pros and cons. The simplicity of its calculation makes it preferable rather than RBC but it only reflects managenent risks of an insurance company. However the RBC classifies exposed risks into management risks, investment risks, interest risks and so on. After thorough quantif7cation of each risk RBC guises net asset the company should preserve. It ig pre-requisite to regulate insurance companies in Korea on the basis of RBC in terms of financial status rather than current European Model as the glob리isation holds us in better financial fitness.

우리나라 소아에서의 β- Lactamase 산생 Escherichia의 장관내 존재

박현수,김경희,조양자,서인수 대한감염학회 1989 감염 Vol.21 No.2

In order to evaluate the possibility that facal E. coli might serve as a reservoir of β-lactamases for Haemophilus influenzae, Neisseria gonorrhoeae, or other members of the Family Enterbacteriaceae, 135 fecal E. coli isolates from Korean children uder two years of age were tested for resistance to ampicillin (??) and for β-lactamases activity. ?? was found in 115(85%) strains of E. coli tested. Of the 115 ?? E. coli, 87(76%) were β-lactamases producers. High rates of ?? and β-lactamases activity were found uniformly in various pathogenic types of E. coli. Our observations raise questions concerning the importance of fecal E. coli in the prevalance of β-lactamase producing bacteria in the community. Fecal E. coli of Korean children may be important in the epidemiology of β-lactamases producing pathogens in humans.

녹농균의 혈청형에 관한 연구

서인수,조양자,정용훈,이열 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.2

The rabbit anti-group sera against thirteen representative standard strains of Pseudomonas aeruginosa, A to M groups were prepared for preparation of serological diagnostic factor. Using the grouping sera for both slide and tube methods, the agglutination reaction has been carried out for each of the thirteen groups of viable and various killed cells of Pseudomonas aeruginosa, A to M groups as agglutinogens to obtain the following results: 1. Of the thirteen anti-group sera eleven showed highly specific antisera for serological typing on the slide agglutination regardless of ind of agglutinogens i.e., viable, thermostable, thermolabile or formalized cells. 2. When serologicalcross reaction of the standard group strains with each of the anti-sera was estimated by using agglutination reaction, the anti-G group serum showed ?? To ?? Intensity of cross reaction with the viable cells of A, F and J grouping. 3. The viable cells of F group were reacted with all anti-group sera except one. 4. The thermostable agglutinogen (120℃, 2hr) of group F strain gave no cross reaction with any of the thirteen anti-group serum. By contrast group M showed the cross reactivity with almost all of the anti-sera. 5. In the course of the immunization, of the thirteen groups, the agglutinin titers of each of anti-group sera, except for the thermolabile antigen were highest in two weeks after immunization by tube method. 6. Of the standard groups strains the group E was highly immunogenic. The anti-E serum showed agglutinin titer 2,560 times more than other groups for various agglutinogens as mentioned previously. The agglutinin titers of anti-C, -D, -D and -K group sera were shown even at 1,280 to 2,560 times dilution when thermostable antigens were used. In case of thermolabile antigens, however, the above titers were not appreciable at 20 times dilution. 7. In the titration of the agglutinins of 0 antigen of Pseudomonas aeruginosa, A to M groups, the thermostable antigens were most sensitive agglutinogen, while the thermolabile antigens were lowest in sensitivity.

한국 성인의 Campylobacter pylori항체에 관한 연구

김기호,김정목,조양자,서인수 한양대학교 의과대학 1988 한양의대 학술지 Vol.8 No.2

Campylobacter pylori (C. pylori) that had recently been reported have becom an interesting objects in the discussion of the etiology of gastritis and peptic ulcer. The role of the C. pylori was evaluated in groups of adults with pathologic mucosa and with normal mucosa, who visited to Hanyang University Hospital in Seoul, Korea. By means of culture or Warthin-Starry silver stain, C. pylori was detected in biopsy materials of 53 (68.8%) out of 77 patients and 1 (7.7%) out of 13 concurrent control subjects (p<0.05). According to the histopathologic gradings, the following numbers were to be positive: active chronic gastritis 41 (75.9%); chronic gastritis 7 (13.0%); chronic ulcer 5 (9.3%); and histologically normal findings 1 (1.9%). A serological screening that used for a dot immunoassay resulted in the following reports: positive Ig G immunoblot were found in 51 (94.4%) of the patients with C. pylori and in 11 (20.4%) of the patients without C. pylori (p<0.01); and positive Ig A immunoblot were found in 11 (20.4%) of the patients with C. pylori and in zero (0.0%) of the patients without C. pylori (p<0.05). A serologic test used for a Western blot immunoassay resulted in seven strong protein bands (108 KD, 98 KD, 78 KD, 72 KD, 68 KD, and 62 KD). These findings strongly implicate C. pylori as one of the cause of active chronic gastritis and chronic ulcer. And this study supports the concept that C. pylori may be an important cause of gastritis or peptic ulcer in Korean adults.

A Successful Pregnancy Following Preimplantation Genetic Diagnosis (PGD) using Primer Extension Preamplification (PEP)-PCR in a Carrier of Duchenne Muscular Dystrophy (DMD)

( Inn Soo Kang ),( Soo Kyung Choi ),( Jin Hyun Jun ),( En Ho Lee ),( Ho Joon Lee ),( Jong Young Jun ) 대한산부인과학회 1996 대한산부인과학회 학술대회 Vol.78 No.-

Linear Ubiquitin Assembly Complex Negatively Regulates RIG-I- and TRIM25-Mediated Type I Interferon Induction

Inn, Kyung-Soo,Gack, Michaela U.,Tokunaga, Fuminori,Shi, Mude,Wong, Lai-Yee,Iwai, Kazuhiro,Jung, Jae U. Elsevier 2011 Molecular cell Vol.41 No.3

<P><B>Summary</B></P><P>Upon detection of viral RNA, retinoic acid-inducible gene I (RIG-I) undergoes TRIM25-mediated K63-linked ubiquitination, leading to type I interferon (IFN) production. In this study, we demonstrate that the linear ubiquitin assembly complex (LUBAC), comprised of two RING-IBR-RING (RBR)-containing E3 ligases, HOIL-1L and HOIP, independently targets TRIM25 and RIG-I to effectively suppress virus-induced IFN production. RBR E3 ligase domains of HOIL-1L and HOIP bind and induce proteasomal degradation of TRIM25, whereas the NZF domain of HOIL-1L competes with TRIM25 for RIG-I binding. Consequently, both actions by the HOIL-1L/HOIP LUBAC potently inhibit RIG-I ubiquitination and antiviral activity, but in a mechanistically separate manner. Conversely, the genetic deletion or depletion of HOIL-1L and HOIP robustly enhances virus-induced type I IFN production. Taken together, the HOIL-1L/HOIP LUBAC specifically suppresses RIG-I ubiquitination and activation by inducing TRIM25 degradation and inhibiting TRIM25 interaction with RIG-I, resulting in the comprehensive suppression of the IFN-mediated antiviral signaling pathway.</P> <P><B>Highlights</B></P><P>► HOIL-1L/HOIP LUBAC suppresses RIG-I-mediated type I interferon signaling ► HOIL-1L/HOIP LUBAC interacts with TRIM25 to induce its degradation ► LUBAC inhibits the K63-linked ubiquitination of RIG-I induced by TRIM25 ► LUBAC interacts with TRIM25 and RIG-I independently, inhibiting their interaction</P>

A pandemic H1N1 influenza virus-like particle vaccine induces cross-protection in mice

Inn, Kyung-Soo,Lee, Gi-Ja,Quan, Fu-Shi Informa Healthcare USA, Inc. 2014 Immunological investigations Vol.43 No.3

<P>Influenza virus-like particles (VLPs) represent promising alternative vaccines. However, it is necessary to demonstrate that influenza VLPs confer cross-protection against antigenically distinct viruses. In this study, a VLP vaccine comprising hemagglutinin (HA) and M1 from the A/California/04/2009 (H1N1) were used and its ability to induce cross-protective efficacy against heterologous viruses A/PR/8/34 (H1N1) and A/New Caledonia/20/99 (H1N1) in mice was assessed. Vaccination with 2009 H1 VLPs induced significantly higher levels of IgG cross-reactive with these heterologous viruses after the second boost compared to after the prime or first boost. Lung virus titers also decreased significantly and the lung cross-reactive IgG response after lethal virus challenge was significantly greater in immunized mice compared to naïve mice. Vaccinated mice showed 100% protection against A/PR/8/34 and A/Caledonia/20/99 viruses with only moderate body weight loss and induction of cross-reactive recall, IgG antibody-secreting cell responses. The variations in HA amino acid sequences and antigenic sites were determined and correlated with induction of cross-protective immunity. These results indicate that VLPs can be used as an effective vaccine that confers cross-protection against antigenically distinct viruses.</P>

Prenatal diagnosis of the spinal muscular atrophy type Ⅰ using genetic information from archival slides and paraffin-embedded tissues

Soo Kyung Choi,Eun Hee Cho,Jin Woo Kim,So Yeon Park,Young Mi Kim,Hyun Mee Ryu,Inn Soo Kang,Jung Young Jun,Je G. Chi 대한의학유전학회 1998 대한의학유전학회지 Vol.2 No.2

Spinal muscular atrophy (SMA) type Ⅰ is a common severe autosomal recessive inherited neuromuscular disorder that has been mapped to chromosome 5q11.2-13.3. The survival motor neuron (SMN) gene, a candidate gene, is known to be deleted in 96% of patients with SMA type Ⅰ. Presently, PCR and single strand conformation polymorphism (PCR-SSCP) analyses have been made possible for application to both archival slides and paraffin-embedded tissues. Archival materials represent valuable DNA resources for genetic diagnosis. We applied these methods for the identification of SMN gene of SMA type Ⅰ in archival specimens for the prenatal diagnosis. In this study, we performed the prenatal diagnosis with chorionic villus sampling (CVS) cells on two women who had, experienced neonatal death of SMA type Ⅰ. DNA extraction was done from archival slide and tissue materials and PEP-PCR was performed using CVS cells. In order to identify common deletion region of SMN and neuronal apoptosis-inhibitory protein (NAIP) genes, cold PCR-SSCP and PCR-restriction site assay were carried out. Case 1 had deletions of the exons 7 and 8, and case 2 had exon 7 only on the telomeric SMN gene. Both cases were found to be normal on NAIP gene. These results were the some for both CVS and archival biopsied specimens, in both cases, the fetuses were, therefore, predicted to be at very high risk of being affected and the pregnancy were terminated. These data clearly demonstrate that archival slide and paraffin-embedded tissues can be a valuable source of DNA when the prenatal genetic diagnosis is needed in case any source for genetic analysis is not readily available due to previous death of the fetus or neonate.

Analysis of haplotype and coamplification PCR dystrophin gene and Y-specific gene using PEP-PCR in single fetal cells

Soo Kyung Choi,Jin Woo Kim,Eun Hee Cho,Hyun Mee Ryu,Inn Soo Kang 대한의학유전학회 1998 대한의학유전학회지 Vol.2 No.1

Duchenne/Becker muscular dystrophy are the major neuromuscular disorders with X-linked recessive inheritance. Preimplantation diagnosis of sex determination has been generally applied to avoid male pregnancies with these diseases. However, in order to determine if the embryo is normal, carrier or affected regardless of the sex, there is a need for a combined analysis of specific exon on dystrophin gene as well as sex determination of embryo using the same biopsied blastomere. If the exon deletion is not determinable, further diagnosis of carrier or patient can be performed by haplotype analysis. in this study, we applied the primer extension preamplification (PEP) method, which amplifies the whole genome, in 40 cases of single amniocyte and 40 cases of chorionic villus cell. We analysed haplotypes using two (CA)n dinucleotide polymorphic markers located at the end of 5' and 3' region of the dystrophin gene. Exon 46 of dystrophin gene and DYZ3 on chromosome Y were chosen as a target sequence for coamplification PCR. Upon optimizing the conditions, the amplification rates were 91.25% (73/80) for haplotypes (92.5% in amniocyte, 90% in chorionic villus cell) and 88.75% (71/80) for coamplification (85% in amniocyte, 92.5% in chorionic villus cell). The result of the study indicates that haplotypes analysis and coamplification of dystrophin and Y-specific gene using PEP can be applied to prenatal and prelmplantation diagnosis in Duchenne/Becker muscular dystrophy making It possible to determine if the fetus is a carrier or an affected one.