Cycloheximide 와 Chloramphenicol 이 보리 유엽의 chlorophyll 및 CP-Complex들의 양적 변화에 미치는 영향

고석찬,허인혹 제주대학교 1984 논문집 Vol.19 No.-

보리 유엽을 greening시킨후 암처리르 행함으로서 chlorophyll함량,CP-comples, 그리고 각 complex에 분포하는 chlorophyll 등의 양적 변화와 이에 미치는CaCl₂, cycloheximide 및 chloramphenic 의 영향을 조사하였다. 암처리에 의하여 chlorophyll a 보다 chlorophyll b 가크게 감소하여 chlorophyll a /b 비율이 높아지고, SHCP의 흡수 peak는 낮아지고 CPI은 높아져서 LHCP/CPI 비율이 감소하였다. 그러나CaCl₂, cycloheximide 및 chloramphenicol들에 의하여chlorophyll b 의 감소가 억제되어 chlorophyll a/b 비율이 낮아지고 LHCP/CPI 비율이 증가하였다. 이러한 사실에서chlorophyll a 와 chlorophyll b,그리고 CPI과 LHCP사이에는 밀접한 상호관계가 있고, 이들 세가지 chemical 들에 의하여 암처리시의 양적 변화를 조절할 수 있으며 단백질 합성과도 밀접한 관계가 있음을 알수 있었다. Dark-growth barley seedlings were exposed for 12hr to continuous light and then the first leaves were incubated with water, CaCl₂,cycloheximide or chloramphenicol for 12hr in the dark. The decrease in chlorophyII b was larger than that of chlorophyII a in the dark, resulting in higher chlorophylly a/b ratio than in the illuminated leaves.As the decrese of chlorophyII b was inhibited in the presence nent peak with a concomitant decrease in LHCP in the dark, but this change of CP-complexes began to disappear when the leaves were incubated with CaCl₂,cycloheximide or chloraphenicol. These results indicate that the changes in the chlorophyll a/b ratio and the CP-complex content of the illuminated leaves in the dark are controlled with CaCl₂and that there is a close relationship between the protein synthesis and the contents of chlorophyll or CP-complexes

무용 전공 여부가 최대 운동 후 혈중 Glucose 및 Insulin 변화에 미치는 영향

서인숙 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

This study was tested in order to produce the metabolic comprehension on efficiency of energy utilization by comparing blood glucose and insulin changes after all-out treadmill run exercise. The subjects of this study were 5 female modern dancers and 5 ordinary female students. The protocol for exercise load was Bruce's stress test, all subjects were ran from rest to all-out state progressively. Blood samples was collected from antecubital vein by using 3 way catheter. The basic design For this study was repeated measurement within group and between groups, independent variables were exercise Conditions and group's property, dependent variables were glucose and insulin All data were analysed by a way ANOVA. The results of this study as follows: 1. On the comparison within each groups, blood glucose concentration increased in immediately exercise, showed the highest level in post-10 min exercise, then suddenly decreased in post- 30 min exercise on both groups with significance, 2. On the comparison between each groups, blood glucose concentration suddenly increased in post- 10 min exercise, than suddenly decreased in post- 30min, exercise in dancer group with significance. 3. On the comparison within each groups, blood insulin concentration significantly decreased in immediately exercise, significantly increased in past-10min. exercise, then significantly decreased in post- 30 min, exercise on both groups. 4. On the comparison between each groups, blood insulin concentration on dance group showed lower level than control group in rest. Dance group showed significantly decreased at immediately exercise, significantly increaser in post-10min, exercise, again decreased in post-30min, exercise than control group.

다발성 중증 외상 환자들의 치료에 대한 응급 외상팀 운영의 효과

이성화 ( Seong Hwa Lee ),조석주 ( Suck Joo Cho ),염석란 ( Seok Ran Yeom ),류지호 ( Ji Ho Ryu ),정진우 ( Jin Woo Jung ),한상균 ( Sang Kyun Han ),김용인 ( Yong In Kim ),박맹렬 ( Maeng Real Park ),김영대 ( Young Dae Kim ) 대한외상학회 2009 大韓外傷學會誌 Vol.22 No.2

Purpose: We performed this study to determine how the emergency trauma team affects the treatment of patients with multiple severe trauma and to discuss the effect and the direction of the emergency trauma team`s management. Methods: We performed a retrospective analysis of 518 patients who visited our emergency department with severe trauma from August 2006 to July 2008. We divided the severe trauma patients into 2 groups: patients before and after trauma team management (Group 1 and Group 2). Then, we compared demographic characteristics, mechanisms of injury, and treatment outcomes (lengths of stay in the ED, admission ratio, and in-hospital mortality) between the 2 groups. In the same way, patients with multiple severe trauma were divided into 2 groups, that are patients before and after trauma team management (Group 3 and Group 4) and analyzed. Results: There was no significant difference, except mean age, between groups 1 and 2. In group 4 patients, compared to group 3 patients, the lengths of stay in the ED were lower (p value<0.001), and the admission ratio were higher (p value=0.017), but there was no significant difference in the in-hospital mortality between the groups 3 and 4. Conclusion: When patients with multiple severe trauma visit the ED, the emergency trauma team`s management can decrease the lengths of stay in the ED and increase the admission ratio, but does not produce a decrease in the in-hospital mortality rate. Further investigations of emergency trauma team management are needed to improve treatment outcomes for patients with multiple severe trauma. (J Korean Soc Traumatol 2009;22:172-78)

조직확장법,술중 조직확장법 및 술전 봉합법의 임상적 응용에 관한 비교 연구

오석준,하지운,김응춘,서인석,조세흠 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.3

The purpose of soft tissue defect reconstruction is minimal scar with aesthetic and functional good results. Reconstruction of head and neck, upper extremity, chest, and lower extremity defects has been reported with standard available tissue expansions. Various surgical methods have been used in an attempt to achieve a normallooking skin color, texture, sensation, thickness and same skin adenexa. Therefore, in accordance with defect size and location, the operative method was considered so we select adequate method. Authors achieved good results from 48 cases of tissue expansion, 18 cases of ISLE (intraoperative sustained limited expansion), and 35 cases of presuturing technique. 1)The most common cause of reconstruction for soft tissue defect was post grafted scar 51cases(51%), next was tattoo 20 cases(19.6%), traumatic soft tissue defect 16cases(15.7% ) and nevus 8 cases(7.8%)in order. 2)Males(55.9%)were affected more often than females(44.1%)in the ratio 1, 3 : 1 and the most frequently affected group was the age of 20 to 30 years as 36% . 3)The distribution of incidence and location were as follow : face(39.2%), upper extremity(25.5%), scalp(17.6%), lower extremity(9.8%) and trunk(7.8%). 4)The cause and distribution were scalp in 20 cases(39.2%), face in 40 cases(29.4%) in the post grafted scar, upper extremity 18 cases(90.0%) in the tattoo, upper extremity 4 cases(50.0%), face 2 cases(25.0%) in the nevus and even distribution in the traumatic soft tissue defects. 5)The presuturing technique was best recommended method for soft tissue defect in 4cm diameter lesion on extremity, but ISLE method was used in the open traumatic soft tissue defect. Over 9cm wide scar was resurfaced by expanded skin using tissue expander.

작은 관동맥 질환의 스텐트 시술 초기 및 추적 결과

당군,손민수,진동규,오세진,손지원,고광곤,최인석,신익균 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.11

5㎜ 단일 부분 절개를 통한 이중 안검 성형술 : Single 5 mm-Partial Incision Method

유현석,금인섭,민경원 大韓成形外科學會 2002 Archives of Plastic Surgery Vol.29 No.6

근관 밀폐도에 미치는 도말층 및 상아질 접착강화제의 영향

양진석,김원재,황인남,오원만 대한치과보존학회 2000 Restorative Dentistry & Endodontics Vol.25 No.4

The purpose of this study was to evaluate effects of smear layer and dentin primers on the sealing ability of root canals. 126 extracted human teeth with single, straight canals and mature apices were used. The Samples were first classified into six groups as follows : presence of smear layer; absence of smear layer; Scotchbond Bond Multi-Purpose; All Bond 2; Mac Bond 2; Clearfil Liner Bond 2. A Positive control was also established. All teeth except the control group were then obturated with thermoplasticized gutta-per-cha and AH26. Electrochemical and dye penetration technique were later used to evaluate the degree of microleakage through the root canal. Seventy teeth were then immersed in a 1 % potassium chloride solution and An external power supply(DC 10 V) was then applied to the circuit for the electrochemical microleakage test. The degree of Microleakage was determined over period of 28 days before being evaluated. In total, 48 teeth were submitted to the dye infiltration technique. All specimen were suspended in 2 % methylene blue dye for 1 week before being longitudinally split. The degree of dye infiltration was measured under a stereomicroscope at×10 magnification and evaluated. The results were as follows: 1.Apical microleakage increased throughout the test period in all group and one group having a smear layer showed a dramatic increase under electrochemical test (p<0.05). In the group having smear layer, the degree of apical microleakage was the highest, and the microleakage was much higher than in the smear layer removed group in electrochemical test (p<0.05). Scotchbond Multi-Purpose, All Bond 2, Mac Bond 2 and Clearfil Liner Bond 2 showed lower microleakage than one group having smear layer. The All Bond 2 and Clearfil Liner Bond 2 treated groups showed the lowest microleakage in electrochemical test (p<0.05). 2.There was no significant difference between the experimental groups in dye penetration technique. These results suggested that the removal of the smear layer from root canal and concomitantly the application of dentin primer into root canal could improve the sealing ability of root canal obturation, 1

개 시상하부의 Neuropeptides에 대한 면역조직화학적 연구

장인엽,정윤영,김종중,문정석,김준수,모근석,임용,유호진 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.1

The distribution and localization of somatostatin(SOM), neuropeptide Y(NPY), vasopressin(VP), and oxytocin(OT) were studied with a immunohistochemical technique in the canine hypothalamus. The SOM-immunoreactive perikarya were concentrated in the anterior periventricular and the arcuate nuclei. Immunoreactive terminals were observed in the arcuate, the ventromedial hypothalamic, the suprachismatic nuclei and the external layer of the median emmence. A great number of NPY-immunoreactive cell bodies were found in the arcuate nucleus and NPY fibers were located throughout the hypothalamus with the highest concentration especially in the paraventricular (PVN) and the acuate nuclei. Moderately or densely stained fibers were also observed in the median eminence, the suprachiasmatic. the periventricular, the ventromedial hypothalamic, the dorsomedial hypothalamic and the medial preoptic nuclei, and the stria terminalis. Both VP-and OT-containing perikarya were found mainly in the PVN and supraoptic nucleus(SON). but a few were seen in the perifornical area, the lateral hypothalamus, the dorsal hypothalamic and the periventricular nucleus. The fibers originated from the PVN were projected to the SON via ventral or dorsal area of the fornix.

각종 성장인자 (GROWTH FACTORS)가 골아 세포주 MC 3T3-E1에 미치는 효과

서인석,이두형 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.2

Bone formation is a complex process which involves a large number of cellular functions directed toward the synthesis and mineralization of bone matrix. Bone formation is regulated by systemic hormones and by systemic and local growth factors which influence bone replication, differentiation and the actual performance of specialized activities. The effects of hormones on bone metabolism have been studied extensively by a number of investigators and, recently, information about growth factors and their role in bone formation and bone resorption has become available. Our laboratory has been interested in the study of factors produced locally by the skeletal tissue. The aim of this study is to investigate the effects of various growth factors on the growth of osteoblastic clone MC 3T3-El cells. Maximum stimulation of proliferation of osteoblastic cells, relative to corresponding controls, was seen at 0.1%-1% serum and the effects was dose-dependent. With various growth factors, such as IGF-Ⅰ,IGF-Ⅱ,EGF, and FGF, sustained the growth of calvarial cells in culture during multiple passages at 0.1% serum. In DNA synthesis assays, all growth factors stimulate cell proliferation. Stimulation of DNA synthesis peaked at 20ng/㎖ of IGF-Ⅰ, 50ng/㎖ of IGF-Ⅱ, and 20ng/㎖ of EGF, while higher concentrations were inhibitory or stationary. PTH-sensitive adenylate cyclase was stimulated by all growth factors. But the alkaline phosphatase activity decreased slowly in cells grown in all growth factors.

"국민윤리" 敎科內容의 模型設定에 關한 一硏究

金渭錫,裵龍光,姜永晧,朴仁熙 慶北大學校 文理科大學 1977 文理學叢 Vol.4 No.-

Based upon the point of view that man would be able to get self-perfection only in community and that, on the other hand, just the man is the origin of vitality of community, writers believe that value education program is needed an useful in the university curriculum. And they found that in substantial character the value education program means the instruction which is not only for a student to adapt himself to the system of the community, but also criticize the system of the community in order to realize the best social conditions. Then, it is important to establish the substantial object of "National Ethics" and to constitute the contents of curriculum of it, which is suitable to accomplish the object of "National Ethics." According to the view mentioned above in this paper, writers attempted to establish a model of contents of "National Ethies" as a university curriculum. Writers think that the substantial object of "National Ethics" must be established with reference to the most important problems for the nation to be solved. Writers believe, therefore, that such problems as ① Primitive Unification, ② Industrialization ③ National Welfare ④ Abundance upon which discoursed A.F.K Organski in his "This Stage of Political Development," or as ① State-Building ② Nation-Building ③ Participation ④ Distribution of which stated G. A. Almond and G. B. Powell in their "Comparative Politics: Developmental Approach," are the most important for us to solve: because the history of modernization of Western Europe would suggest an End-State model for the underdeveloped nations. The following conclusions were derived from the present study: First, a statue of a human being as the substantial object of "National Ethics" is one who eager to solve and accomplish such problems mentioned above. Second, according to the suggestions of the history of modernization of Western Europe, the contents of curriculum of "National Ethics", which would be able to accomplish the substantial object of the "National Ethics," must be constituted with the contents which cultivate the patriotism. And from the history of modernization of Western Europe writers find out the facts that such modern spirits as positivism, rationalism and so forth rested on the basis of their traditional thought. And so, Koreanic modern spirits must be derived from her traditional thought. Then writers conclude that the contents of "National Ethics" must be constituted with "Korean thought" and the thought of "Chyung-Hyo" which is one of the Korean traditional morals. Third, writers concluded that it is reasonable to exclude the chapter of "The Philosophy of Political Science" and "Criticism of Communism" from the contents of curriculum of "National Ethics," which is contained in present text of "National Ethics," and to treat each one as a independent subject if it is possible. Fourth, a system of "Korean Thought" which is stated in this paper is an example for further study.