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임사비나,임형택,박히준,장지련,최일환,이석찬,김대수,신희섭 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-
Objective : Acupuncture has been used for treatment of numerous disorders, especially for pain control in Oriental Medicine. However, the mechanism of pain control by acupuncture was not clear until now. This study was performed to prove analgesic mechanism of acupuncture treatment at acupoint ST_(36) by observing the changes of abdominal pain and c-Fos expression in the thalamus. Methods : Abdominal pain was induced by acetic acid, and the changes of writhing reflex after acupuncture treatment an ST_(36) and non-acupoints were measured. c-Fos immunohistochemistry was also performed to study the changes of the neuronal activity in the thalamus. Results : The writhing reflex decrease significantly after acupuncturing at ST_(36) compared with control group(p<0.05). The changes of the writhing reflex by non-acupoint acupuncture treatment also showed significant decrease compared with control group(p<0.05). c-Fos expression in the thalamus, especially periventricular part was significantly decreased after acupuncturing at ST_(36) compared with control groups(p<0.05). Conclusion : This study shows that the acupuncture has the analgesic effect in the abdominal pain induced by acetic acid and the thalamus might be a important area for this mechanism.
Effects of Apolipoprotein A-I on Apoptosis and Cytokine Production in Human Neutrophils
Hyung-Kon Kang(강형곤),Jae-Hyung Choi(최재형),Jae-Taeck Huh(허재택) 한국생명과학회 2011 생명과학회지 Vol.21 No.1
아포지방단백 A-I (apoA-I)은 항염증 및 항산화 작용을 가지고 있는 것으로 알려져 있다. 본 논문에서는 인간혈액의 호중구를 이용하여 in vitro 상태에서 세포사멸과 시토카인의 분비 과정에 미치는 apoA-I의 영향을 조사하였다. 인간 호중구에 apoA-I을 처리한 결과 세포사멸의 정도가 감소되었다. 또한, apoA-I을 함유하는 고밀도 지방단백(HDL)에 의하여도 세포사멸은 현저히 감소되었다. HDL이 결합하는 수용체인 scavenger 수용체 B-1에 대한 항체에 의하여도 세포사멸은 억제되었다. ApoA-I을 20 시간 처리한 세포의 배양액내 인터루킨-8, 인터페론-유도 단백질 10(IP-10) 및 종양괴사인자-α의 분비가 현저히 증가하였다. 호중구에 인터페론 감마와 apoA-I을 동시 처리하였을 때 시토카인 중에서 IP-10과 종양괴사인자-α의 분비가 단독으로 처리한 경우에 비해 현저히 증가되었다. 이러한 결과들은 인간 혈액 호중구에 apoA-I을 처리한 경우 호중구가 활성화되고 호중구의 세포사멸이 지연된다는 것을 제시하는 것으로 apoA-I이 호중구에 대하여 염증성 인자로 작용할 것으로 추정된다. Apolipoprotein A-I (apoA-I) has anti-inflammatory and anti-oxidative properties. This study was designed to investigate whether apoA-I affects apoptosis and cytokine production of human blood neutrophils in an in vitro culture system. Spontaneous apoptosis of neutrophils was significantly delayed by apoA-I. In addition, high density lipoprotein containing apoA-I also delayed apoptosis of neutrophils. Apoptosis of neutrophils was inhibited by anti-scavenger receptor type B-I antibodies. The amounts of interleukin-8, interferon (IFN)-inducible protein 10 (IP-10), and tumor necrosis factor-α (TNF-α) in the supernatants of cultured neutrophils treated with apoA-I were significantly increased. Combined treatment of neutrophils with IFN-γ and apoA-I produced higher amounts of IP-10 and TNF-α than did treatment with IFN-γ or apoA-I alone. The present study reveals that apoA-I activates neutrophils to produce cytokines and delays spontaneous apoptosis of neutrophils. These findings suggest that apoA-I, although a well-known negative acute-phase protein, has a pro-inflammatory effect in neutrophils.
Mesangial 세포에 대한 고농도 포도당의 영향 및 PDGF 수용체억제제의 효과
형근영,박병현,박승택,조정구 圓光大學校 醫科學硏究所 2001 圓光醫科學 Vol.16 No.1-2
배경: 신세포의 비대를 매개하는 대표적인 성장인자들은 TGF-β외에도 IGF-1, PDGF(platelet derived growth factor, 혈소판 유래 성장인자), EGF, FGF 등이 있으며 혈관활성물질인 엔지오텐신 Ⅱ(Angiotensin Ⅱ) 등도 당뇨병성 신증 발생에 관여한다는 보고가 있다. 그중 PDGF는 강력한 mitogen으로 메산지움세포와 같은 간질(mesenchymal) 기원 세포에 대하여 강한 화학친화성(chemoattractant)을 보이며 메산지움세포의 증식이 나타나는 동물 및 사람의 사구체신염에서 발현되어, 당뇨병성 신증 발생 시 나타나는 메산지움 세포의 증식과 관련 가능성에 관심이 모아져있다. 그러나 PDCF, TGF-β 등의 사이토카인 들 사이의 작용 및 메산지움세포에 대한 영향은 배양세포의 종류 및 밀도, 처리한 사이토카인이 내인성 인지 외인성으로 투여된 것인지, 배양액 내 혈청의 존재유무, 다른 사이토카인의 활성화 여부등에 따라 결과가 다르게 보고되어 사이토카인의 작용에 복잡성이 있고 특히 메산지움 세포 증식에 미치는 TGF-β의 영향에 대한 연구는 매우 부족하고도 서로 상반된 결과를 보이고 있는 실저이다. 방법: 저자는 당뇨병성 신증의 병인 규명 및 치료에 관한 연구의 일환으로 고농도 포도당 배지에서 PDGF 수용체 억제제를 사용하여 생쥐 배양 메산지움세포에 미치는 PDGF의 영향을 알아보고 TGF-β와의 상호 연관성을 알아보고자 본 연구를 시행하였던 바 다음의 결과를 관찰하였다. 결과: 5mM 포도당 농도에 비해 10mM 및 30mM 등 고 포도당농도에서 TGF-β 처리가 지속적으로 메산지움세포 증식을 유발하여 당뇨병성 신증의 발생 및 진행에 중요한 역할을 할 것으로 생각되었다. 또한 PDGF 수용체 억제제인 suramin 을 TGF-β와 함께 처리하자 TGF-β를 단독 처리했을 때 관찰되었던 메산지움세포의 증식이 차단되었다. 결론: 당뇨병성 신증의 진행에 관여하는 성장인자들은 서로 자가분비 또는 측분비의 형태로 서로 매개 작용을 하는 것으로 보이며 고농도 포도당상태에서의 TGF-β의 메산지움 세포에 대한 작용과정에 PDGF가 매개 역할을 하고 있는 것으로 생각된다. Background : Diabetic nephropathy is characterized by hypertrophy of both glomerular and tubular elements, thickening of the glomerular and tubular basement membranes, progressive accumulation of extracellular matrix components in mesangial cells, and tubulointerstitial fibrosis. Hyperglycemia increases the level of diacylglycerol(DAG) and activates protein kinase C(PKC) in mesangial cells and other vascular tissues. PKC activation regulates a number of vascular functions such as vascular permeability, contractility, cellular proliferation, basement membrane synthesis, signal transduction mechanisms for hormones and growth factors. In addition, glomerular mesangial cells play an important role in the development of diabetic nephropathy. Mesangial cells have several functions such as contractile properties, phagocytosis of macromolecules, synthesis of matrix proteins, and production and response to growth factors like platelet derived growth factor(PDGF), transforming growth factor-β(TGF-β). Also, these growth factors play important roles in mesangial cell proliferation and pathophysiology of diabetic nephropathy. Specifically, TGF-β is a key mediator in development of diabetic nephropathy. Methods : In order to clarify the effect of high glucose concentration and the relation- ship between PDGF and TGF-β on mouse mesangial cell proliferation, hyperglycemia was induced by the addition of high glucose to medium containing mesangial cells derived from neonatal mouse. The effect of high glucose was assessed by the cell proliferation, after mesangial cells were induced with various concentrations of glucose for 48 hours. The effect of TGF-β and PDGF on cell proliferation was examined and the effect of an inhibitor of PDGF and its receptor, suramin was also examined. Results : 1. LC_50 was a concentration of 20mM glucose when mesangial cells were cultured for 48 hours. 2. Hyperglycemia decreased cell number after mesangial cells were incubated for 48 hours with media containing 20-25 mM glucose, respectively. 3. TGF-β increased cell number dose-dependently. 4. PDGF induced a significant cell proliferation, but suramin, an inhibitor of PDGF receptor decreased in cell number in dose-dependently. 5. In the cell number, suramin decreased in number of mesangial cells against TGF-β induced cell proliferation. Conclusion : High glucose induced toxic effect, so it was manifestated by decreased number of cultured mesangial cells, and PDGF receptor inhibitor decreased cell proliferation which increased by TGF-β in high glucose. This means PDGF may modulate TGF-β effect on mouse mesangial cell proliferation in high glucose concentration.
Fate and Survivability of Fish Bacteriophage Inoculated in BALB/c Mice
Ji Hyung Kim,Kyung Taeck Lim,Dennis K. Gomez,Gun Wook Baeck,Gang Joon Heo,Se Chang Park 한국실험동물학회 2006 Laboratory Animal Research Vol.22 No.4
Understanding of bacteriophage kinetics in mammals or higher organisms is needed, as this determines the potential phage activity in antibacterial treatment. We demonstrated the fate and survivability of fish phage particles in different organs and fluids inoculated into mice. Phage (PPpW-4) specific to Pseudomonas plecoglossicida was intraperitoneally injected to mice with a dose of 10<SUP>8.1</SUP> PFU/㎖/mouse. Phage titers (10<SUP>3.0</SUP>~10<SUP>5.2</SUP> PFU/g) were detected in blood and other organs within 10 min after injection except in the urine samples. Phage titers in blood (10<SUP>5.1</SUP>~10<SUP>6.9</SUP> PFU/g) were 10~100 times higher than that of the other organs from 10 min until 24 h. It shows that phage was distributed quickly to the different organs by circulatory system via the blood. Phage titers in all organs showed the highest level in 1 h (10<SUP>4.2</SUP>~10<SUP>6.9</SUP> PFU/g) and reduced rapidly until no phage was detected in 72 h. Although phage disappeared in blood within 72 h, phage can still survived in the spleen and kidney until 96 h. Spleen in particular, had an appreciable phage titer of 10<SUP>2.7</SUP> PFU/g. There were comparatively high phage titers (10<SUP>3.0</SUP>~10<SUP>4.7</SUP> PFU/g) in urine from 1 h up to 24 h. It means that one possible mechanism of phage elimination is by urinary excretion.
IP-RFID를 활용한 해양레저 활동관리시스템 적용에 관한 연구
최형림(Choi, Hyung-Rim),박병권(Park, Byung-Kwon),박용성(Park, Yong-Sung),이창섭(Lee, Chang-Sup),조용현(Jo, Yong-Hyun),임성택(Lim, Sung-Taeck),하정수(Ha, Jeong-Soo),이병하(Lee, Byung-Ha) 한국산학기술학회 2011 한국산학기술학회논문지 Vol.12 No.2
본 오늘날 경제적 발전과 Well-being에 대한 요구가 확산되면서 다양한 여가 문화가 발달하고 있다. 그 중에 서도 3면이 바다인 한반도의 지리적 특성으로 인해 해양레저 시장이 지속적으로 성장하고 있다. 이러한 해양레저 시 장과 이용자들의 증가에도 불구하고, 안전 관리와 장비 관리, 서비스 관리 등은 체계적으로 수행되지 못하고 있다. 해 양레저 활동은 해상에서 이루어지기 때문에, 육상에서 이루어지는 레저 활동보다 더욱 충돌, 조난과 같은 사고에 의 한 피해가 크다. 그리고 해양레저 장비는 대부분 고가이기 때문에 체계적으로 관리되는 것이 필요하다. 본 연구에서는 IP-RFID라는 새로운 기술을 이용하여 해양레저 활동을 즐기는 이용자들의 안전 관리와 장비 관리를 체계적으로 수행할 수 있는 정보시스템을 제시한다. With current economic advancements and increasing demand for well-being, diverse leisure cultures have developed. The geographic feature of being surrounded in 3 sides by water has resulted in the sustained growth of the marine leisure market. But the increased market and users has not resulted in the systematic management of sailing safety and equipment. Marine leisure activities occur on water, so the damage from collisions and distress calls are greater than land based leisure activities. And marine leisure equipment is very expensive, requiring systematic management. This research proposes an information system using a new IP-RFID technique to manage the safety of marine leisure users and equipment management.
만성 척수손상 환자에 대한 대망전위수술 : 예비보고 Preliminary Report
허재택,김형동,최휴진,김기욱 대한신경외과학회 1991 Journal of Korean neurosurgical society Vol.20 No.7
The omentum has been used over the years for a variety of clinical problems. Recently it has shown that placing the omemtum on the brain and spinal cord can lead to an extensive development of vascular connections at the omental/CNS interface. Success with omental transposition to the human brain has led to increasing intreast in placing the omentum onto the human spinal cord. One paraplegic patient was chosen to enter into a feasibility study to see if omental transposition to their spinal cord might result in clinical benefit. The length of time from injury was about 21 months respectively. This patient had little, if any, motor and sensory function below umbilicus level. The operation required surgical lengthening of the pedicled omentum followed by its placement into a subcutaneous tunnel created backward along the lateral chest wall up to T-10, 11 level. An extensive thoracic laminectomy was then performed followed by a wide opening of the dura. In our case the cord showed segmental shrinkage of the spinal cord consistent with previous trauma. The omentum was laid directly onto the underlying spinal cord. Our observation for five months has shown that placement of the omentum onto the chronically injured spinal cord allowed for subsequent improvement in neuroelectrical activity, as manifested by reproducible somatosensory evoked potentials, and, more importantly, in moter function. It is considered that placing the omentum directly upon the brain or spinal cord may have the effect of either improving local vascular perfusion or, possibly, exerting some biochemicals(neurotransmitter), or as yet unknown, influence. But further observation is warranted to determine whether this improvement will be continued and will be observed in another cases.
Choi, Jae-Hyung,Park, Hyun-Seok,Kim, Dae-Hyun,Cha, Jae-Kwan,Huh, Jae-Taeck,Kang, Myongjin The Korean Neurosurgical Society 2015 Journal of Korean neurosurgical society Vol.57 No.5
Background : Higher reperfusion rates have been established with endovascular treatment for acute ischemic stroke patients. There are limited data on the comparative performance of mechanical thrombectomy devices. This study aimed to analyse the efficacy and safety of the stent retriever device (Solitaire stent) by comparing procedure time, angiographic outcome, complication rate and long term clinical outcome with previous chemical thrombolysis and mechanical thrombectomy using penumbra system. Method : A retrospective single-center analysis was undertaken of all consecutive patients who underwent chemical thrombolysis and mechanical thrombectomy using Penumbra or Solitaire stent retriever from March 2009 to March 2014. Baseline characteristics, rate of successful recanalization (modified Thrombolysis in Cerebral Infarction score 2b-3), symptomatic intracerebral hemorrhage, procedure time, mortality and independent functional outcomes ($mRS{\leq}2$) at 3 month were compared across the three method. Results : Our cohort included 164 patients, mechanical thrombectomy using stent retriever device had a significant impact on recanalization rate and functional independence at 3 months. In unadjusted analysis mechanical thrombectomy using Solitaire stent retriever showed higher recanalization rate than Penumbra system and chemical thrombolysis (75% vs. 64.2% vs. 49.4%, p=0.03) and higher rate of functional independence at 3 month (53.1% vs. 37.7% vs. 35.4%, p=0.213). In view of the interrelationships between all predictors of variables associated with a good clinical outcome, when the chemical thrombolysis was used as a reference, in multiple logistic regression analysis, the use of Solitaire stent retriever showed higher odds of independent functional outcome [odds ratio (OR) 2.62, 95% confidence interval (CI) 0.96-7.17; p=0.061] in comparison with penumbra system (OR 1.57, 95% CI 0.63-3.90; p=0.331). Conclusion : Our initial data suggest that mechanical thrombectomy using stent retriever is superior to the mechanical thrombectomy using penumbra system and conventional chemical thrombolysis in achieving higher rates of reperfusion and better outcomes. Randomized clinical trials are needed to establish the actual benefit to specific patient populations.