Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

Kim, Hyung-Keun,Song, Mina,Jun, ji-Hae,Woo, Kyung-Mi,Kim, Gwan-Shik,Baek, Jeong-Hwa The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone merabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and 1,25(OH)_(2)vitaminD_(3)(VD3) by using mouse oligo 11 K gene chip. In the presence of 10 mM[Ca^(2+)]e or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expressions of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high ectracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated;slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated;s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracelluar calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.

형광 in situ hybridization 방법에 의한 c-myc 유전자의 전위 및 증폭에 관한 연구

김경채,박선화,김강련,김미영,전용혁 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

중앙아시아 벽화 보존처리(1) : 벽화(본4074, 본4096)의 상태조사

강형태,이용희,유혜선,김연미,조연태,시고 아오키,노리코 야마모토,켄타로 오바야시 국립중앙박물관 2001 박물관보존과학 Vol.3 No.-

국립중앙박물관은 용산 새 박물관의 전시 유물로 선정된 중앙아시아 벽화의 연차적인 보존처리를 위해 “독립행정법인 일본 도쿄문화재연구소’’와 공동연구를 모색하게 되었다. 2001년에 본4074, 본4096 작은 벽화편 2점의 보존처리를 시작하면서 벽화의 균열 박락 등 손상상태, 구조와 구성재료, 이전 보존처리에서 적용된 재료와 방법에 대한 기초조사를 실시하였다. 조사결과 본4074, 본4096 2점의 벽화는 흙과 지푸라기를 반죽하여 만든 벽체에 석고를 발라 바탕으로 하고 그 위에 채색을 한 것으로 나타났으며 벽체 속에 포함된 지푸라기의 방사선 탄소연대측정 결과 본 벽화는 10세기 말에서 13세기 초에 만들어진 것으로 나타났다. 또한 X-선회절 분석 결과 화면의 흰색 바탕은 gypsum[Ca(SO₄)·2H₂O]과 CaSO₄, Calcite(CaCO₃)가 적색계통은 연단(鉛丹:Pb₃O₄)과 led arsenate[Pb(As₂O₆)], 녹색계통은 Cuprite(Cu₂O)와 arsenolite(As₂O₃), arsenic oxide(As₂O₄) 등이 사용된 것으로 조사되었다. This article is about a joint project carried out by the National Museum of Korea and the Tokyo Cultural Properties Research Institute for the conservation of central Asia Wall painting that has been selected for the exhibition at the new Seoul National Museum of Korea at Yongsan. The investigation of the wall painting revealed very useful information. This includes the condition of the object, and the identification of evident damage, such as cracks, loss of pigment, plus materials and methods employed during the object's creation, as well as previous conservation treatment. The object was mainly made by applying plaster to the body (wall) that consisted of a mixture of soils and rice straws. Then, on the surface of the wall-painting, pigments were used to draw and to colour it. As a part of the investigation, radiocarbon dating was conducted using straw samples taken from the object. The result indicates that the object is probably dated form between the end of the 10th Century and the beginning of the 13th Century. The result of X-ray diffraction also revealed the composition of the pigments used on the surface. These are 1. gypsom[Ca(SO₄)·2H₂O], CaSO₄ and Calcite(CaCO₃) and Calcite(CaCO₃) that were used for the white background. 2. Pb₃O₄ and led Arsenate [Pb(As₂O₆) that were used for the red colouring. 3. Cuprite(Cu₂O), Arsenolite(As₂O₃) and Arsenic Oxide(As₂O₄) that were used for the green colouring.

황남대총 98호분 출토 유리의 과학적 분석

조경미,유혜선,강형태 국립중앙박물관 1999 박물관보존과학 Vol.1 No.-

황남대총 98호분 북분과 남분에서 출토된 유리시료 40점에 대한 과학적인 분석을 실시하였다. 유리의 성분조성은 주사전자현미경-에너지분산형분광기(SEM-EDS)를 사용하여 정량분석 하였고 다변량해석법을 통하여 시료를 분류하였다. 그 결과 시료 40점 모두 Na₂O를 약 20%정도 함유한 소다-석회(Na₂O CaO-SiO₂)유리임을 확인하였으며, 다시 5개 주성분(SiO₂, AI₂O₃, Na₂O, CaO, K₂O)으로 다변량해석[주성분분석(PCA)]을 실시한 결과 2개의 群으로 분류되었다. I 群에 포함된 시료는 Al₂O₃의 농도가 9.7%로 높고 CaO의 농도는 2.2%인데 비하여 Ⅱ群에서는 각각 3.2%,4.9%의 범위로 나타났다. 특히 시료 No.12의 노란 색으로 편석된 부분을 미소부위 XRD로 분석한 결과 PbSnO₃임을 국내 최초로 확인할 수 있었다. 鉛을 함유한 시료 중 No.12, 17은 열이온화질량분석기(TIMS)로 납동위원소비를 측정하였고 선형판별식 분석법(SLDA)을 이용하여 납의 산지를 추정하였다. 그 결과 각각 중국 남부, 한국 남부의 납광석을 사용한 것으로 나타났다. Elemental analysis of 40 glass samples from the Northern Tomb and the Southern Tomb of Hwangnam-daechong No. 98 was performed. Fourteen compositions of each sample were analyzed quantitatively by SEM-EDS and glass samples were classified by multivariate analysis such as PCA. All of 40 samples were confirmed to be Na₂O CaO-SiO₂ system with about 20% of Na₂O. Samples were classified into two groups by doing PCA on concentrations of 5 major elements (SiO₂, AI₂O₃, Na₂O, CaO, K₂O). Samples included in group I showed the concentration of AI₂O₃ is about 9.7% and that of CaO, about 2.2%. In group II, concentration of Al₂O₃ is about 3.2% and that of CaO, about 4.9%. Especially yellow grains embedded in sample No.12 were shown to be PbSnO₃ by micro XRD, which was the first coloring material ever found in Korea. Lead isotope ratios of samples No.12 and No.17 which contained lead were measured by TIMS. The origin of lead was traced by means of multivariate analysis such as SLDA. The result showed that lead from southern China and southern Korea had been used for making glass.

웹 어플리케이션 서버 기반의 인트라넷 시스템 설계 및 구현

이형묵,윤영미 京畿專門大學 1999 京畿專門大學 論文集 Vol.- No.27

Internet has been applied to information technology business and extends to the whole industrial world. As the most effective way of information propagation in an organization, implementing an intranet system makes a distinctive progress and various implementation methodology has been introduced. Our study proposed a solution for the intranet system commanding an university administrial and educational tasks making a comparison and an analysis among various intranet system implementation technologies, identified business requirements processes, implemented the intranet system based on this framework, and applied the system to our college.

GMM을 이용한 통화론적 환율결정모형의 추정

金美亨 全北産業大學校 産業經營硏究所 1997 産業經營硏究 Vol.5 No.-

This Article attempts to forecast monthly won-dollar exchange rates using GMM. The study addresses several issues. The first issue is whether Bilson's monetary exchange rate model estimated with OLS and GMM outperforms a random walk model. The second one is whether the different exchange rate regimes such as the monetary basket system and the market average exchange rate system that is a floating exchange rate system with a daily exchange rate change limit influence the goodness-of-fit of the monetary model. The third one is whether the size of the daily exchange rate change limit causes the forecasting ability of the models to differ. The fourth one is whether the size of the estimation window affects the forecasting power. The sample period is from January 1970 through June 1996. The results can be summarized as follows. First, Bilson's monetary exchange rate model estimated with GMM outperforms a random walk model in out-of-sample forecasting power. The forecasting ability of the monetary model estimated with OLS is, however, confounding. It is better than random walk model in the criteria of MAE but worse in RMSE. Second, exchange rate volatility has significantly decreased after monetary exchange rate system has changed to market average exchange rate system. The monetary exchange rate model estimated with GMM, however, is a good statistical model in two different exchange rate regimes respectively. Third, the forecasting ability of the exchange rate model differs depending on the size of the daily exchange rate change limit. The bigger a change limit, the bigger a forecasting error based on RMSE and MAE. Most of time, the monetary model outperforms a random walk model. Fourth, extending the estimation period, the forecasting error increases. It indicated that the coefficients of the monetary model are time-variant.

적혈구 유무에 따른 벤젠, 스티렌, 트리클로로에틸렌에 의한 림프구 중 자매염색분체 교환 빈도

성재혁,김형아,신민정,최미정,이세훈 가톨릭대학교 산업의학센터 2001 韓國의 産業醫學 Vol.40 No.4

This study was performed to investigate the possible role of oxyhemoglobin in the metabolic activation of benzene, styrene and TCE by observing the difference of the SCE frequency between isolated lymphocyte and whole blood lymphocyte cultures exposed to these chemicals. Isolated lymphocyte and fresh heparinized peripheral whole blood samples from a healthy donor(male, smoker) were cultured in the phytohaemagglutinin stimulated culture media for 72 hours. The cells were treated with 100, 300, 1000, 3000 μM benzene, 250, 500, 100, 2000 μM styrene or TCE respectively. Slides were stained with Giemsa's solution for SCE. SCE was analyzed for each subject from coded slides by one researcher. The results were as follows : 1.The frequency of SCE increased dose-dependently with concentration of benzene in isolated lymphocyte and whole blood lymphocyte cultures, however there were no significance. There was no significant difference of SCE frequency between isolated lymphocyte and whole blood lymphocyte cultures. 2.The frequency of SCE in whole blood exposed to sytrene significantly increased in dose-dependant relationship, but not in isolated lymphocyte. The difference of SCE frequency between isolated lymphocyte and whole blood lymphocyte of 2000μM styrene exposure was marginally significant(P=0.0540). 3.In TCE, the frequency of SCE did not increase at any concentration used in this study of isolated lymphocyte and whole blood lymphocyte. Above results suggested that oxyhemoglobin in erythrocytes play an important role in the metabolic activation of styrene but not in benzene or TCE. Key Words : Benzene, Styrene, Trichloroethylene, Sister chromatid exchange, Metabolic activation, Oxyhemoglobin

정보처리유형에 따른 유아의 이야기 구성능력의 차이

오미형 한국아동교육학회 2005 아동교육 Vol.14 No.1

본 연구에서는 유아의 정보처리 양식에 따라 유아가 구성하는 이야기의 구조와 길이에 차이가 있는지를 살펴보고자 하였다. 연구의 대상을 표집하기 위해 한국판K-ABC 지능검사를 실시하여 유아의 정보처리 유형에 따라 동시처리형 11명과 순차처리형 15명을 무선 표집하였다. 이야기 구성 검사 도구로 글없는 그림책을 사용하였으며 이야기구성력과 이야기의 길이를 측정하였다. 이야기구조와 이야기길이를 종속변인으로 하고 유아의 정보처리양식을 독립변인으로 하여 MANOVA를 실시하였다. 연구결과 유아가 구성한 이01기의 구조와 길이는 유아의 정보처리 유형에 따라 차이가 있는 것으로 나타났다. The purpose of this study was to examine the differences of Story Construction according to children with a strength in sequential processing or simultaneous processing. For sampling the subject of this study, Korean version K-ABC Intelligence Test(Moon, Soo-Back, 1997)was conducted 96 children aged 5 who were attending B kindergarten in U city. As a result of this test, 15 children of sequential processing style and 15 children of simultaneous processing style were sampled randomly. As for test tool, 'story construction from a picture book' which was made by Kraayenoord & Paris, was modified, used. Data was analyzed by MANOVA according to problems. Followings are the results of this study: Firstly, there was no significant difference between sequential processing style and simultaneous processing style in 'metalinguistics' of story construction, but in cases of 'comments about pictures' and 'elaboration and personal involvement', the children group of sequential processing style got statistically higher grades than the children group of simultaneous processing style. And in cases of 'revision strategies' and 'theme/moral' of story construction, the children group of simultaneous processing style got statistically higher grades than the children group of sequential processing style. Secondly, there was significant differences between children's information processing style in story length. That is, the children group of sequential processing style made longer story than the children group of simultaneous processing style.

인트라넷 환경에서 E-R Diagram에 기반한 Interactive Form Generator의 설계 및 개발에 관한 연구

김형미,조미선,승현우 서울여자대학교 자연과학연구소 1998 자연과학연구논문집 Vol.10 No.-

Currently, considerable work has gone into the construction of the Intranet which utilizes the advantages of the World-Wide Web and enables the enterprize to easily accomodate the huge amount of information and work already built. In this paper, we propose an interactive forms database query language which enables users to build their queries visually and naturally in the 'fill-in-the-blank' manner. The previous forms interface like Oracle Forms are static in the sense that forms are already set in the templates, while the proposed forms interface is dynamic since users are able to create their own forms from the E-R(Entity-Relation-ship) Diagram. We also propose an E-R Diagram Generator upon which the forms templates are built.

C형 간염의 병리조직학적 고찰

김형준,김재규,김미경,박중원,유병철,박실무 중앙대학교 의과대학 의과학연구소 1997 中央醫大誌 Vol.22 No.2

After the discovery of hepatitis C virus, several reports of histologic charcteristics of hepatitis C have been published. The aim of this study is to evaluate histopathologic features of hepatitis C for diagnostic significance. Needle biopsy specimens obtanied from the liver of 14 patients with hepatitis C were evaluated for the diagnostic significance. All patients had not any evidence of hepatitis B, and autoimmune and alcoholic liver diseases. The specimens were histologically evaluted based on previously reported features of hepatitis C such as bile duct damage, lymphocytes infiltration, steatosis and so on. According to the mode of transmission, sporadic infections were 12 cases, posttransfusion hepatitis and Ⅵ drug abuser was 1 case. The commonst finding was sinusoidal inflammatory cell activation, especially lymphocyte (86%). And lymphoid follicles or lymphocytes aggregates (65%), steatatosis (65%) were found. According to the activity of hepatitis, significant difference of pathologic features could not be proposed, but most histologic features were showned in CAH. In conclusion, all the histologic characteristics of hepatitis C such as sinusoidal cell activation, lymphoid aggregates, steatosis were observed in this study. But the further study of comparative histologic evaluation between hepatitis C and other hepatitis is needed. And also, comparative study with larger subjects for specific histopathologic findings according to the activity of hepatitis and epidemiologic cause in needed.