Classical Swine Fever (Hog cholera) Virus 약독순화주 (Suri 주)의 gp55 Gene 염기서열 분석

김귀현,장경수,강경임,이병형,박종현,안수환,전무형 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

An attenuated classical swine fever virus (CSFV), Suri strain, is a variant derived from a vaccine virus, LOM strain. This study was performed to elucidate the molecular biologcal properties of CSFV Suri strain, and to obtain the basic data for molecular epidemiological approaches for the disease. The truncated form of gp55 gene without the C-terminal trasmembrane domain, in size of 1,023bp, was amplified by RT-PCR and sequenced by dye terminator cyclic sequencing method, and inserted into BamHI site of pAcGP67B baculovirus vector, establishing a cloned pAcHEG plasmid. By the nucleotide sequences determined, 341 amino acid sequences were predicted. As compard the nucleotide and amino acid sequences of gp55 of Suri with the various CSFV, Suri strain showed the high homology over 99.1% with ALD and LOM strains, but comparably the lower homology with Alfort and Brescia. In comparison of amino acid sequence in variable domain of gp55 protein, the similar tendency of homology was observed. In hydrophobicity analysis, all of four CSFV strains revealed the analogous patterns of hydrophobicity. The numbers and locations of N-glycosylation site and cysteine residuse in gp55 were analyzed, those of Suri strain being coincident with ALD and LOM strains. The results suggest that gp55 in Suri strain has the high similarity to those in ALD and LOM strains in terms of the nucleotide and amino acid sequences and the functional properties of gp55 protein.

꿩에서 분리된 Newcastle Diesase Virus 내열성주 (CBP)의 Fusion(F) 유전자 클론닝과 염기서열 분석

장경수,전무형,송희종,김귀현,박종현 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

The gene encoding F protein of CBP-1 strain, a heat-stable Newcastle disease virus (NDV) isolated from the diseased pheasants in Korea, was characterized by reverse transcriptionpolymerase chain reaction (RT-PCR), nucleotide and amino acid sequences. Virus RNA was prepared from the chorioallatoic fluid infected with NDV CBP-1 virus and cDNA was amplified by RT-PCR, cloned and sequenced to analyze. The PCR was sensitive as to detect the virus titer above 2^5 hemagglutination unit. 1.7kb (1,707bp) size of the cDNA was amplified and cloned into BamHI site of pVL1393 Baculo transfer vector. The uncleotide sequences for F protein were determined by dye terminator cyclic sequencing using four pairs of primers, and 553 amino acid sequences were predicted. In comparison of the nucleotide sequence of F gene of CBP-1 with those of other NDV strains, the homology revealed 88.8%, 98.5% and 98.7% with Kyojungwon (KJW), Texas GB and Beaudette C strains, respectively. As the deduced 553 amino acid sequences of F protein of CBP-1 were compared with those of other NDV strains, the homology appeared 89.9%, 98.7% and 98.9% with KJW, Texas GB and Beaudette C strains, respectively. The putative protease cleavage site (112-116) was R-R-Q-K-R, indicating that CBP-1 strain is velogenic type. The amino acid sequences include 6 sites of N-asparagine-linked glycosylation and 13 cysteine residues. These data indicate that the genotype of CBP-1 strain is more closely associated with the strains of Texas GB and Beaudette C than KJW strain.

감나무탄저병에서 분리한 Gloeosporium kaki의 2계통

崔貞植,李貴宰,金炯武,蘇仁永 全北大學校 1993 論文集 Vol.35 No.-

A anthracnose symptom at persimmon tree was found out in the field, Chonju in Chonbuk province and Haenam Experiment Station in Chonnam province in 1991 and 1992. The cultivated character and the pathogenicity of the fungi were as follows : These fungi, Gloeosparium kaki which were isolated from the anthracnose were classified into strain Ⅰ and Ⅱ by the cultivated characteristics. The size of conidia in strain Ⅰ was 22.5 × 6.82 ㎛ and that of strain Ⅱ was 20.3 × 6.2 ㎛. In a PDA, the colour of strain I was light brown and made a central zone. The colour of strain Ⅱ was black brown and deep grey and didn't make a central zone. The pathogenicity of strain Ⅰ was similar to strain Ⅱ in the leaf, branches.

계태아연속계대에 의한 내열성 Newcastle disease virus 분리주의 약독화

박정우,장경수,정용성,김귀현,송희종,전무형 충남대학교 수의과대학 동물의과학연구소 1997 動物醫科學硏究誌 Vol.5 No.-

In the cases of Newcastle disease(ND) outbreaks in the pheasant farms located in Chungchung area, five heat-stable Newcastle disease viruses(NDV) have been isolated from the diseased pheasants, and the various biological properities of the isolates have been reported. In this study, we attempted attenuation of a heat-stable NDV isolate, CBPI by serial subpassages in SPF chick-embryo and examined its pathogenicity and immunogenicity. When CBPI isolate was subpassaged by the 153rd levels, the heat stability was not changed as compared with that of the parent virus. However, its virulence was reduced according to increasing level of subpassage. The chicken infected with the attenuated CBPI at age of 1, 2 and 5 weeks showed no mortality with mild respiratory signs in all age groups tested, and significantly decreasing body weight gains in the 5 week-old chicken, while parent virus showed 100% mortality in all age groups. In comparison of adverse reactions between the attenuated CBPI and B1 strain in 1 week-old chicken with NDV maternal antibody(HI antibody titer>1:8). both viruses manifested no specific clinical signs, but significantly lower body weight gain in the group of chicken inoculated with CBPI. When the attenuated CBPI or B1 strains were inoculated into the 3 week-old chicken vaccinated primarily with B1 strain, both of viruses showed no specific side effects and changes in body weight. However, the HI antibody titers in the chicken inoculated with the attenuated CBPI were slightly higher than that of the chicken inoculated with B1 vaccine.

최근 충청지역 환축으로부터 분리된 병원세균의 약제 감수성

장경수,정용성,김귀현,전무형 충남대학교 수의과대학 동물의과학연구소 1997 動物醫科學硏究誌 Vol.5 No.-

During the past three years, the antimicrobial susceptibility for the major pathogenic bacteria isolated from the swine herds and dairy farms located in Chungchong area has been examined. The major bacterial species isolated from the respiratory lesions of the diseased pigs during the periods were B. bronchiseptica, Haemopilus spp, P. multocida, Streptococcus spp., A pleuropneumonia. And from the fecal specimens of the pigs with diarrhoea, E. coli, Salmonella spp., Campylobacter spp., Streptococcus spp. and Clostridium spp. were isolated and investigated for the drug susceptibility. The major isolates from the milk samples of the dairy cattle with mastitis were Staphylococcus spp., Streptococcus spp., E. coli, Pseudomonas spp., Corynebacterium spp., Clostridium spp. and P. multocida. The total of 311 isolates were examined for 24 kinds of antimicrobial drugs. Of the isolates from the swine respiratory lesions, B. bronchiseptica and Haemophilus spp. showed 83% to 92% of suscepibility to ciprofloxacin(CIP), amikacin(AN) and norfloxacin(NOR). The isolates of P. multocida revealed 80% to 90% of susceptibility to AN, CIP, NOR and gentamycin(GM) and all of A pleuropneumonia isolates, susceptible to AN, CIP, GM, kanamycin(K), NOR and sulfamethoxazole-trimethoprim(SXT). Of the isolates from the pigs with diarrhoea, most isolates of E. coli showed the high susceptibility to AN, CIP and GM. And Salmonella spp. revealed 71% to 94% of susceptibility to GM, cephalothin(CF), AN and CIP, Campylobacter spp., 79% to 95% of susceptibility to AN, CIP and GM, and all of Clostridium spp., to AN, CIP, GM and information(FM), Of the isolates from the dairy cattle, Staphylococcus spp. and Streptococcus spp. showed 81% to 95% of susceptibility to AN, CF, CIP, FM, and AN, CIP, SXT, FM, K, respectively. E coli isolates showed 81% to 94% of susceptibility to CIP, AN, FM, and Pseudomonas spp., 83% to 94% of susceptibility to AN, AM and CIP. Campylobacter spp. and Clostridium spp. showed 89% and 100% of susceptibility to CIP, respectively. In measuring the MIC range of the major isolates for the drugs showing high sensitivity, the isolates from swine showed ≤0.06-64 ㎍/㎖ in CIP, 0.06-128 ㎍/㎖ in NOR and 0.5-256 ㎍/㎖ in AN, and the pathogens from the dairy cattle, ≤0.06-128 ㎍/㎖ in CIP, 0.25-256 ㎍/㎖ in AN and 0.12-512 ㎍/㎖ in NOR.

忠南地域 屠畜豚의 肺病變으로부터 分離한 Actinobacillus pleuropneumoniae의 生物學的 및 免疫學的 特性

이종훈,장경수,장치훈,김일택,정용성,김귀현,전무형 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

An epidemiologic study on pleuropneumonia in the slaughter pigs(Chonan and Asan area, Chungnam province, Korea) during the period of January 1994 through December 1995 was conducted. Isolation of A. pleuropneumoniae was attempted in 425 pigs with pneumonic lesions. Biochemical properties, antimicrobial susceptibility, serotypes and pathogenicity of A. pleuropneumoniae isolates were investigated. In addition, outer membrane protein(OMP) of the isolates were extracted to determine its properties and immunogenicity in both mice and piglets. Of 3,395 slaughter pigs, pleuropneumonia was observed in 425 pigs(10.6%). A. pleuropneumoniae was isolated from 22 pigs(5.2%) out of 425 pigs with pneumonic lesions. The biochemical properties of all isolates were same as those of reference A. pleuropneumoniae strain. Among 22 isolates, 9, 1 and 12 isolates were serovar 2, 3 and 5, respectively. The results of antimicrobial susceptibility test revealed that the isolates showed high susceptibility to ciprofloxacin and cephalothin, moderate susceptibility to amikacin, gentamicin, kanamycin and streptomycin, and low susceptibility to erythromycin, tylosin and sulfadimethoxin. The isolates were varied in pathogenicity to mice. Median lethal dose of LE9402(serovar 2) and LE9511(serovar 5) were 9.2×10^7CFU and 2.8×10^7CFU, respectively. Specific pneumonic lesions were observed from the infected mice with clinical signs. Bacteria recovery rate was high in the lung, but low in heart blood and tracheas. Serovar 2 was found to be more pathogenic than serovar 5 in guinea pig. Mortality on guinea pigs inoculated with serovar 2(5.4×10^8 - 5.4×10^6CFU) abd serovar 5(2.8×10^8 - 2.8×10^6CFU) was 20-40% and 40-80%, respectively. A severe hemorrhagic lesions and focal pneumonic lesions were observed from dead guinea pigs. Bacteria recovery rate was relatively higher in the lung than that of other organs. In the SDS-PAGE analysis, OMP-enriched fractions of both isolates and reference strains contain common peptide bands equivalent to molecular weight of 17, 27, 42, 52 and 95Kd. In addition to common peptide bands, the bands which are specific to each isolate were also observed. The profiles of Sephadex G25 fractions showed 3 major peaks. The common peptide bands which were observed by SDS-PAGE of the crude OMPs were found i the peaks 1 and 2. The OMPs extracted from serovar 2(LE9402) and serovar 5(LE9511) provided high level of protection in mice(70-80%) and pigs(100%). All animals inoculated with OMPs were seroconverted, showing micro-agglutination titer of 640 to 1280.

제 3대구치 발치 후 발생한 돌발성 난청: 증례보고 및 문헌 고찰

김형기(Hyung Ki Kim),김일형(Il-hyung Kim),구정귀(Jeong-Kui Ku),노민호(Min-Ho Noh) 대한치과의사협회 2020 대한치과의사협회지 Vol.58 No.7

This study reports the unusual complications of 22-year-old male who presented with sudden hearing loss after the right mandibular third molar extraction under local anesthesia with 3.6 ml of 2 % lidocaine. Total 8.75 ㎎ of oral dexamethasone for 1 week immediately after extraction was prescribed in department of oral and maxillofacial surgery but hearing did not improve after 1 week. As referral to otolaryngology, total 600 ㎎ of oral methylon and hyperbaric oxygen therapies were operated for 2 weeks. The hearing of patient was improved at 6 weeks after extraction but tinnitus was persisted even after 12 months. The reason and treatment were discussed with literature review, searching with the keywords [‘hearing loss’ AND (‘dental’ OR ‘tooth extraction’OR‘teeth extraction’)] in PubMed and Google scholar at October 2019. Total five cases were reported after tooth extraction with local anesthesia. The sudden hearing loss could be associated with local anesthesia containing vasoconstrictors. Early steroid (extensive medication and intra-tympanic injection) and hyperbaric oxygen therapies were recommended within 2 weeks. As a proper treatment, hearing could be improved but other additional symptoms, such as tinnitus, dizziness, might be remained.

The Mediation Effect of Herd Behavior on the Impact of Trust on Purchase Intention: A Focus on Livestream E-commerce in China

Fan Zhou(Fan Zhou),Ki-Hyung Bae(Ki-Hyung Bae),De-Kui Li(De-Kui Li) 한국무역연구원 2023 무역연구 Vol.19 No.2

Characteristics of Tobacco Mosaic Virus Isolated from Wasabi(Eutrema wasabi) in Korea

Hyung Moo Kim,Kui Jae Lee 한국식물병리학회 1999 Plant Pathology Journal Vol.15 No.4

Uterotrophic Assay Using Ovariectomized Female Rats with Sub-cutaneous Administration

( Hyung Sik Kim ),( Soon Young Han ),( Rhee Da Lee ),( Kwang Sup Kil ),( Kui Lea Park ) 한국응용약물학회 2000 Biomolecules & Therapeutics(구 응용약물학회지) Vol.8 No.1