일본 개설서와 사전류의 개항기 기술 분석

현명철(Hyun, Myung?Cheol) 한일관계사학회 2006 한일관계사연구 Vol.25 No.-

2,4-D 處理가 'Harcot' 살구와 '美白' 복숭아의 果實 成熟과 品質에 미치는 影響

윤철구,김영호,임상철,김학현,이철희,최관순,김선규 충북대학교 농업과학기술연구소 1999 農業科學硏究 Vol.16 No.-

Effect of 2,4- D application on the fruit maturity and quality of 'Harcot' apricot and 'Mibaek' peach was examined. Leaf area of 'Harcot' apricot was not affected by 2,4-D while that of 'Mibaek' peach was increased by 35mg · L-1 2,4-D. Application of 2,4-D at 35mgㆍL-1 increased the fruit weight of both species, and that of 'Harcot' was doubled. Soluble solids content of 'Harcot' was decreased by 2,4-D while that of 'Mibaek' was not affected. Fruit maturity of 'Harcot' and 'Mibaek' was enhanced for respective 4 and 1 day by 2,4-D application. Percent fruit cracking of 'Harcot' apricot was decreased by 35mg · L-1 2,4-D.

연골외막세포가 포함된 alginate beads를 이용한 토끼에서의 골연골결손 치료

안현정,김은미,정미현,조현철,이인범,성상철,이명철 대한정형외과연구학회 2003 대한정형외과연구학회지 Vol.6 No.2

지베렐린과 에세폰의 葉面撒布가 '美白' 복숭아의 熟期와 品質에 미치는 影響

김영호,윤철구,임상철,김학현,이철희,최관순,김선규 충북대학교 농업과학기술연구소 1999 農業科學硏究 Vol.16 No.-

In order to obtain the basic data for artificial maturity control of 'Mibaek' peach, GA and ethephon were foliar sprayed 4 weeks prior to harvest, and their effects on fruit maturity and quality were examined. GA and ethephon tended to decrease the fruit weight except 100mg ㆍ L-1 GA+50 to 100mg · L-1 ethephon treatment. GA increased the fruit firmness, and ethephon at higher concentration increased the fruit drop. Harvest date was advanced for 7 to 8 days by ethephon while was delayed for 6 to 8 days by GA treatment, regardless of concentration.

Alginate 배양내에서 Transglutaminase가 연골막세포에 미치는 영향

박정선,공차배,김충현,안현정,정미현,성상철,김희중,조현철,이상훈,이명철 대한정형외과연구학회 2003 대한정형외과연구학회지 Vol.6 No.2

뉴로-퍼지 논리를 이용한 12경락의 양도락진단 시스템의 구현

김병화,한권상,이우철,김성현,최해성,안현식,김도현 대한전자공학회 1997 秋季綜合學術大會 論文集 Vol.20 No.2

법랑모세포 분화와 법랑질 형성과정에서 OD314, Apin protein의 발현 및 기능

박종태,최용석,김흥중,정문진,오현주,신인철,박주철,손호현 대한치과보존학회 2006 Restorative Dentistry & Endodontics Vol.31 No.6

본 연구에서는 법랑모세포 분화와 법랑질 형성에 연관이 있는 OD314 일명 Apin protein의 기능을 밝힐 목적으로, in-situ hybridization에 의한 OD314 mRNA 발현과 법랑모세포 세포주에서 OD314 enamel matrix protein의 발현, 그리고 OD314 유전자를 과발현/억제시킬 수 있는 construct를 제작한 후 법랑질 형성 중에 OD314의 기능을 알아보고자 RT-PCR를 시행하여 다음과 같은 결과를 얻었다. 1. OD314 mRNA는 발생중인 상아모세포보다 법랑모세포에서 강하게 발현되었다. 2. Tuftelin은 석회화 결정이 형성되는 14일까지 발현이 지속되고, 그 이후부터 점차 감소하였다. Amelogenin과enamelin은 7일부터 그 발현이 점점 감소하였다. 3. U6-OD314 siRNA construct를 이용하여 transfection한 법랑모세포 세포주는 OD314와 tuftelin,MMP2 mRNA 발현이 감소하였으며, CM-OD314를 transfection하여 OD314의 과발현을 유도한 경우에는 OD314와 MMP20 mRNA의 발현이 뚜렷이 증대되었다. 이 결과는 OD314가 법랑모세포의 분화와 법랑질의 형성 그리고 석회화 과정에 중요한 역할을 하는 새로운 인자임을 시사한다. This study was aimed to elucidate the biological function of OD314 (Apin protein), which is related to ameloblast differentiation and amelogenesis. Apin protein, calcifying epithelial odontogenic (pindborg) tumors (CEOTs)-associated amyloid, were isolated from CEOTs, and has similar nucleotide sequences to OD314. We examined expression of the OD314 mRNA using in-situ hybridization during tooth development in mice. Expression of OD314 and several enamel matrix proteins were examined in the cultured ameloblast cell line up to 28 days by reverse transcription-polymerase chain reaction (RT-PCR) amplification. After inactivation and over-expression of the OD314 gene in ameloblast cell lines using U6 vector-driven RNA interference and CMV-OD314 construct, RT-PCR were performed to evaluate the effect of the OD314 during amelogenesis. The results were as follows: 1. In in-situ hybridization, OD314 mRNAs were more strongly expressed in ameloblast than odontoblast. 2. When ameloblast cells were cultured in the differentiation and mineralization medium for 28 days, the tuftelin mRNA expression was maintained from the beginning to day 14, and then gradually decreased to day 28. The expressions of amelogenin and enamelin were gradually decreased according to the ameloblast differentiation. 3. Inactivation of OD314 by U6-OD314 siRNA construct down-regulated the expression of OD314, MMP-20, and tuftelin, whereas over-expression of OD314 by CMV-OD314 construct up-regulated the expression of OD314 and MMP-20 without change in tuftelin. These results suggest that OD314 is considered as an ameloblast-enriched gene and may play the important roles in ameloblast differentiation and mineralization.

프롤린 함량 증가 및 내건성 강화 관련 새로운 RING-Type E3 유비퀴틴 리가아제 애기장대 atrzf1 돌연변이체의 생리적 기능 연구

주현우,민지희,정문수,김철수 전남대학교 농업과학기술연구소 2021 농업생명과학연구동향 Vol.59 No.-

The covalent attachment of ubiquitin to proteins plays a fundamental role in the regulation of cellular function through biological events involving abiotic of biotic stress responses, immune responses, and apoptosis, Here, we characterize the biological function of the Arabidopsis thaliana RING Zinc Finger 1 (AtRZF1) in dehydration response. AtRZF1 was significantly reduced by drought stress. The atrzf1 mutant was less sensitive to osmotic stress than the wild-type during early seedling development, wheareas transgenic pants overexpressing AtRZF1 were hypersensitive, indicating that AtRZF1 megatively regulates drought-mediated control of early seedling developmeng. Moreover, the ectopic expression of the AtRZF1 gene was very significantly influential in drought sensitiv parameters including proline content, water loss, membrance ion leakage and the expression of dehy-dration stress-relat genes. ARZF1 is a functional E3 ubiquitin ligase, and its conserved C3H2C3-type RING domain is likely important for the biological function of AtRZF1 in drought response. Together, these results suggest that the E3 ligase AtRZF1 is an important regulator of water deficit stress during early seedling development.

한국인 알코올의존환자에서의 제1형 부신피질자극 호르몬 분비 촉진 호르몬수용체의 유전자 다형성에 관한 연구

김철민,김성곤,김지훈,김현경,김미경,유정현,민은정 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

스트레스 반응에 중요한 역할을 하고 있는 CRHR1 유전자 SNP와 알코올 의존과의 연관성에 관한 선행 연구들이 있다. 이에 본 연구는 백인을 대상으로 조사했던 기존연구와는 달리 한국인 남녀를 대상으로 하여 알코올 의존군과 정상 대조군의 CRHR1 유전자형 빈도 및 대립유전자형 빈도를 비교하였다. 본 연구의 연구대상자들은 알코올 의존군 268명(남자 218명, 여자 50명)과 정상 대조군 137명(남자 78명, 여자 59명)이었다. CRHR1 유전자 SNP를 SNP database와 선행연구를 근거로 선정한 후, 연구 대상자들의 이러한 SNP 유전자형을 PCRRFLP방법으로 조사하였다. 그 결과, 한국인의 CRHR1 유전자 SNPs로 7개(rs242938, rs404623, rs28364027, rs16940686, rs937, rs878886, rs878887)가 분석되었다. 이중 rs28364027의 A 대립 유전자의 빈도는 남녀 각각에서 알코올 의존군이 대조군보다 유의하게 높았다. 그리고 rs28364027의 AA 유전자형 및 A 대립 유전자와 rs878886의 CC유전자형 및 C 대립유전자의 빈도는 알코올 의존군내에서 여성이 남성보다 유의하게 높았다. 이러한 결과를 종합해 보면 CRHR1 유전자 SNP가 알코올 의존의 유전적 원인의 중요한 요인일 가능성을 제시하고 있다. 또 이러한 CRHR1 유전자 SNP가 알코올 의존의 유전적 원인에서 남녀간 차이를 설명할 수 있을 가능성도 시사하고 있다. Objectives:There were several preceding studies investigating the association between alcohol dependence and corticotropine releasing hormone receptor1(CRHR1) gene SNPs that play an important role in response to stress. The frequencies of CRHR1 genotypes and alleles were compared between alcohol-dependent patients and normal control subjects. Methods:The subjects were 268 alcohol-dependent patients(218 males, 50 females) and 137 normal controls(78 male, 59 female). CRHR1 gene SNPs were investigated according to the SNP database and the results from previous studies, and their genotypes were analyzed by polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP). Results:Seven CRHR1 gene SNPs(rs242938, rs404623, rs28364027, rs16940686, rs937, rs878886, rs878887) were found in Korean subjects. The frequency of rs28364027 A allele was significantly higher in alcohol-dependent patients than normal controls in both genders. In alcohol-dependent patients, the frequencies of AA genotype and A allele of rs28364027, and of CC genotype and C allele of rs878886 were higher in female than male. Conclusion:These results suggest that CRHR1 gene SNP is one of the important genetic factors in the etiology of alcohol dependence. And also it is supposed that the different frequency of SNP genotype could explain stress-related gender difference in the genetic etiology of alcohol dependence.

수열합성 반응에 의한 3mol % 지르코니아 세라믹의 Fe2+, Fe3+, Er3+치환에 따른 상변태와 물성 변화

변지철, 이현철, 김택남, 류재경 배재대학교 공학연구소 2017 공학논문집 Vol.19 No.1

Zirconia is a heat-resistant material with a high melting temperature(2700℃) and has low thermal conductivity and excellent corrosion resistance. However, application has been limited due to brittleness, which is a disadvantage of ceramic materials. As a way to improve this, the addition of Y3+ improves the strength by phase transformation, which can improve the brittle problem, which is a disadvantage of ceramic. Many studies have been conducted on the addition of Y3+ after the addition has been found to be more efficient than the other additives. In this experiment, have studied the additives that replace this Y3+. Expeiments on the possibility of replacing Y3+ by adding an alternative additive considering Zr-sol(Fe2+, Fe3+, Er3+) in consideration of Atomic size, Crystal ionic raddi and Effective Ionic raddi. ZOC(ZrOCl2*8H2O) was dissolved in distilled water and synthesized by hydrothermal synthesis at 160℃ for 24hours. The formed Zr-sol was separated into a supernatant and precipitate. The supernatant was washed with distilled water, and the presence of chloride ion was confirmed using AgNO3. And add to the cleaned Zr-sol an additive that is believed to be a substitute for Y3+. The prepared Zirconia was calcined at 900℃ for 2hours, and the crystal phase was analyzed by XRD similar that of YSZ. The calcined powder was uniaxial pressed and sintered at 1500℃ for 2hours. The sintered specimens were first measured for specific gravity. And the particle size distribution, EDAX and FESEM were measured. 지르코니아는 고온에선 용융하는 (2700℃) 낮은 열전도율과 부식저항이 우수한 세라믹이다. 이에 우수한 파괴인성을 주기 위해 3 mol % Y를 첨가하게 되고, 상온에서 정방정 상으로 존재함으로써 파괴인성이 증가한 것으로 알려져 있다. 따라서 본 실험에서는 Y와 유효 원자 반경이 유사한 Er3+ (89pm)를 첨가함으로써, 과연 어떤 변화가 나타나는지를 알아보았다. 또한 원자가가 유사한 Fe3+와 Fe2+ 를 첨가하여 물성변화와 상변화를 조사하였다. 연구결과 이온 반경이 Y와 유사한 Er3+에서는 완전한 정방정상과 밀도, 투광율 (일부 파장은 제외)을 나타내었으나, 이온 반경이 약1/2 인 Fe2+, Fe3+를 첨가한 경우에는 단사정과 비정질 그리고, 650nm이하의 파장에서는 거의 투광하지 않는 현상을 보였다.