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Kim, Hyoun-Ee,Kim, Hae-Won,Jang, Jun-Hyeog John Wiley Sons, Ltd. 2009 Journal of Peptide Science Vol.15 No.1
<P>Heparin/heparan sulfate (HS) plays a key role in cellular adhesion. In this study, we utilized a 12-mer random Escherichia coli cell surface display library to identify the sequence, which binds to heparin. Isolated insert analysis revealed a novel heparin-binding peptide sequence, VRRSKHGARKDR, designated as HBP12. Our analysis of the sequence alignment of heparin-binding motifs known as the Cardin–Weintraub consensus (BBXB, where B is a basic residue) indicates that the HBP12 peptide sequence contains two consecutive heparin-binding motifs (i.e. RRSK and RKDR). SPR-based BIAcore technology demonstrated that the HBP12 peptide binds to heparin with high affinity (K<SUB>D</SUB> = 191 nM). The HBP12 peptide is found to bind the cell surface HS expressed by osteoblastic MC3T3 cells and promote HS-dependent cell adhesion. Moreover, the surface-immobilized HBP12 peptide on titanium substrates shows significant increases in the osteoblastic MC3T3-E1 cell adhesion and proliferation. Copyright © 2008 European Peptide Society and John Wiley & Sons, Ltd.</P>
Case Report : An Antinuclear Antibody-Negative Patient With Lupus Nephritis
Hyoun Ah Kim,Jae Wook Chung,Han Jung Park,Dai Yeol Joe,Hyun Ee Yim,Hae Sim Park,Chang Hee Suh 대한내과학회 2009 The Korean Journal of Internal Medicine Vol.24 No.1
Systemic lupus erythematosus (SLE) is a typical autoimmune disease that`s characterized by various autoantibodies to nuclear and cytoplasmic antigens. The presence of antinuclear antibodies (ANA) in serum is generally considered a decisive diagnostic sign of SLE. However, a small subset of SLE patients who had the typical clinical features of SLE was reported to show persistently negative ANA tests. Our report describes a 16-yr-old female who presented with the clinical manifestations of SLE such as malar rash, photosensitivity, arthritis, lymphopenia, pericarditis and proteinuria. The serum autoantibodies were all negative and renal biopsy showed that the histopathological changes of immune complex mediated the focal segmental necrotizing glomerulonephritis with crescent formation. She was treated with monthly pulse cyclophosphamide along with corticosteroids. During the 2-yr follow-up period, the proteinuria was markedly decreased and all of the ANA and anti-double stranded DNA antibody tests were negative. This case suggests that ANA may not be required in the pathogenesis of lupus nephritis. (Korean J Intern Med 2009;24:76-79)
류마티스관절염 환자에서 Bucillamine에 의한 신병증
김현아 ( Hyoun Ah Kim ),임현이 ( Hyun Ee Yim ),성준모 ( Jun Mo Sung ),이진우 ( Jin Woo Lee ),서창희 ( Chang Hee Suh ) 대한류마티스학회 2009 대한류마티스학회지 Vol.16 No.3
Objective: Bucillamine is a disease-modifying antirheumatic drug that`s widely used in Korea and Japan, and it is reported to be a cause of proteinuria. However, the clinical course of the nephropathy associated with the use of bucillamine in rheumatoid arthritis patients has not been described in detail in Korea. Methods: We examined clinical records of 835 patients who were treated with bucillamine for rheumatoid arthritis at least 2 months at Ajou University Hospital from 2003 to 2008, and we found 23 patients (2.75%) with proteinuria. Each patient was followed up until the proteinuria had resolved. Results: At the time the proteinuria developed, the mean age of patients was 53.8±11.0 years. Only one patient had marked hypoalbuminemia (<3.0g/dL). The mean value of the random urine protein-creatinine ratio was 3.44±2.99. The proteinuria appeared 4∼18 months after the initiation of the treatment with bucillamine. Among the patients, renal biopsy was carried out in 18 patients, and pathological findings were 17 cases of membranous glomerulopathy and 1 case of focal segmental glomerulosclerosis. On the follow-up of the 18 patients, the proteinuria in all the patients had resolved completely without deterioration of renal function. But the time to resolution of the proteinuria was positively correlated with the length of bucillamine treatment after the onset of proteinuria (p<0.001, r=0.744). Conclusion: Prevalence of proteinuria in patients receiving bucillamine was 2.75%, and bucillamine-induced nephropathy showed a good prognosis in Korea. The most important thing for resolving the bucillamine-induced proteinuria is to discontinue the bucillamine.
Hydroxyapatite-Based Biomaterials for Hard Tissue Applications
Kim Hae-Won,Kim Hyoun-Ee The Korean Society of Medical and Biological Engin 2005 의공학회지 Vol.26 No.5
Over the past few decades, much effort has been made to improve the mechanical and biological performance of HA, in order to extend its range of applications. As a major inorganic component of human hard tissues, hydroxyapatite bioceramic is regarded as being one of the most biocompatible materials. Numerous in vitro and in vivo studies have confirmed its excellent bioactivity, osteoconductivity and bone forming ability. However, because of its poor mechanical properties, its use in hard tissue applications has been restricted to those areas in which it can be used in the form of small sized powders/granules or in the non-load bearing sites. A number of researchers have focused on improving the mechanical and biological performance of HA, as well as on the formulation of hybrid and composite systems in order to extend its range of applications. In this article, we reviewed our recent works on HA-based biomaterials; i) the strengthening of HA with ceramic oxides, ii) HA-based bioactive coatings on metallic implants, iii) HA-based porous scaffolds and iv) HA-polymer hybrids/composites.
Kong, Young-Min,Kim, Hyoun-Ee,Kim, Hae-Won Wiley Subscription Services, Inc., A Wiley Company 2008 Journal of biomedical materials research. Part B, Vol.b84 No.2
<P>In this study, we report a new observation on the phase conversion that occurs during the sintering of hydroxyapatite (HA)–tricalcium phosphate (TCP) biphasic ceramics. During the sintering of the HA-TCP mixture powders, a large amount of TCP was converted into HA, as detected by X-ray diffraction. The amount of TCP transformed into HA was ∼10–90% of that initially added. From the electron probe microscopy analysis, the HA transformed from TCP was found to be Ca-deficient with Ca/P ratios of 1.62–1.64. The dissolution behavior and osteoblastic responses in a series of HA-TCP biphasic ceramics (10–90% TCP) were assessed. The solubility of the HA-TCP biphasic ceramics was intermediate between that of the HA and TCP pure ceramics. However, in the case of the HA-90% TCP biphasic ceramic, the solubility was even higher than that of pure TCP. The cell proliferation and alkaline phosphatase activity of the cells on the biphasic ceramics were lower than those on pure HA, but higher than those on pure TCP. However, particularly in the HA-50% TCP biphasic composition, the cellular responses were significantly higher than those on pure HA. It is considered that the Ca-deficient apatite newly formed from the TCP may affect in some way the solubility and biological properties of the HA-TCP biphasic ceramics. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2008</P>
Kim, Hae-Won,Kim, Hyoun-Ee,Salih, Vehid,Knowles, Jonathan C. Wiley Subscription Services, Inc., A Wiley Company 2005 Journal of biomedical materials research. Part A Vol.a74 No.3
<P>Titanium (Ti) surface was coated with hydroxyapatite (HA) films via the sol-gel method. The coating properties, such as crystallinity and surface roughness, were controlled and their effects on the osteoblast-like cell responses were investigated. The film crystallinity was controlled with different heat treatment temperatures (400, 500, and 600°C): Also the surface roughness was changed by using different heating rates (1 and 50°C/min). The obtained sol-gel films had a dense and homogeneous structure with a thickness about 1 μm. The film heat-treated at higher temperature had enhanced crystallinity (600 > 500 ≫ 400°C), while retaining similar surface roughness. When heat-treated rapidly (50°C/min), the film became quite rough, with roughness parameters being much higher (4–6 times) than that obtained at a low heating rate (1°C/min). The dissolution rate of the film decreased with increasing crystallinity (400 ≫ 500 > 600°C), and the rougher film had slightly higher dissolution rate. The attachment, proliferation, and differentiation behaviors of human osteosarcoma HOS TE85 cells were affected by the properties of the films. On the films with higher crystallinity (heat treated over 500°C), the cells attached and proliferated well and expressed alkaline phosphatase (ALP) and osteocalcin (OC) to a higher degree as compared to the poorly crystallized film (heat treated at 400°C). On the rough film, the cell attachment was enhanced, but the ALP and OC expression levels were similar as compared to the smooth films. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005</P>
Collagen-apatite nanocomposite membranes for guided bone regeneration
Song, Ju-Ha,Kim, Hyoun-Ee,Kim, Hae-Won Wiley Subscription Services, Inc., A Wiley Company 2007 Journal of Biomedical Materials Research Part B Vol. No.
<P>Collagen-apatite nanocomposite is regarded as a potential biomaterial because of its composition and structure, which are similar to those of human hard tissues. However, there have been few investigations of its mechanical and biological benefits in direct comparison with a collagen equivalent. Herein, we successfully produced a biomedical membrane made of a nanocomposite, and systemically evaluated the mechanical, chemical, and biological properties of the nanocomposite in comparison with those of pure collagen. The results showed that significant improvements were achieved by the nanocomposite approach, particularly in terms of the mechanical strength and chemical stability. The present findings point to the potential usefulness of the collagen-apatite nanocomposite membrane in the field of guided bone regeneration (GBR). © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007</P>