이스라엘잉어 장관 수축에 관여하는 약물수용체에 관한 연구

윤효인,한경호,박승춘,조준형,오태광,Yun, Hyo-in,Han, Kyong-oh,Park, Seung-chun,Cho, Joon-hyoung,Oh, Tae-kwang 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.3

In order to elucidate the characterization of receptors involved in inestinal motility of Israeli carp, spontaneously contracting Israeli carp intestinal preperations were prepared and mounted in the organ chambers for contraction traicings using a polygraph. Various contractile agonists were treated and their dose-response curves were constructed. $EC_{50}$ values$(pD_2)$ of each agonist on specific receptors, $pA_2$ values of competitive antagonists against some agonists, and $K_1$, values of noncompetitive antagonists against some agonists were analyzed for characterization of receptors related with the intestinal contraction. Results obtained through the experiments were summarized as follows: 1. Acetylcholine(ACh) exhibited biphasic dose-response curves: initial ACh-induced dose dependent contractions were observed in pM levels but followed by decreased response in in-between concentration levels. Dose dependent contractions reappeared in ${\mu}M$ level. The peaks in pM and ${\mu}M$ levels appeared in $10^{-13}M$ and $3{\times}10^{-5}M$, respectvely. 2. Carbachol(CaCh) exhibited dose dependent contractions from $10^{-9}M$ to $10^{-5}M$, and its $pD_2$ values were higher than those of ACh($5.60{\pm}0.11$). ACh and CaCh exhibited equiactive contractions. Nicotine had no effects on contractile responses of Israeli carp intestine. 3. ACh-induced responses were inhibited by atropine($K_1:7{\times}10^{-8}M$), a muscarinic antagonist, in a non-competitive manner. But CaCh-induced responses were inhibited by both antimuscarinic atropine($pA_2:9.52{\pm}0.14$) and selective $M_2$ antagonistic 4-DAMP($pA_2:8.16{\pm}0.09$), in competitive manners. Nicotine receptor antagonistic decamethonium and hexamethonium had no effects on ACh-and CaCh-induced contractions. Therefore, the cholinergic receptor related to intestinal motility of Israeli carp was assumed as $M_2$ type. 4. In Israeli carp intestine, 5-HT (serotonin) exhibited dose dependent contractions in concentration range from $10^{-8}M$ to $10^{-5}M$. The maximal responses, however, were corresponded to about 50% of those of ACh or CaCh. 5-HT induced contractions were inhibited by $5-HT_2$ antagonistic ketanserin ($K_1: 7.8{\times}10^{-4}M$) in a non-competitive manner, but not by both of anti $5-HT_1$, spiperone and anti $5-HT_3$, MDL-72222. Hence, $5-HT_2$ receptors are suggested to be existed in Isreli carp intestine.

Use of insulin detemir in dogs with diabetes mellitus

Hyo-Mi Jang,Na-Young Eom,Yang-Ho Kang,Dong-In Jung 충북대학교 동물의학연구소 2015 Journal of Biomedical and Translational Research Vol.16 No.1

Insulin detemir is a long-acting basal insulin analogue recently introduced in veterinary medicine for treatment of canine diabetes mellitus. As there are only limited studies in dogs, long-term evaluation of insulin detemir in veterinary medicine is required. In this study, we investigated trends in12-hour blood glucose concentration during hospitalization and evaluated initial and following doses of insulin detemir for several months in six diabetic dogs. The mean levels of blood glucose over 12-hour periods were between 113.5 to 327.2 mg/dL, and the average glucose nadir was 103 mg/dL in the six dogs. The dogs were treated with a mean dosage of 0.24 U/kg of insulin detemir, but hypoglycemia was observed in four of the dogs at the first monthly follow-up. Thus, insulin doses were adjusted according to the nadir levels of glucose observed during the follow-up periods (range, 1 to 16 months). The total range of insulin doses throughout the study period was between 0.1 and 0.4 U/kg. Changes in insulin doses in each dog during the follow-up period were not variable. We suggest that insulin detemir might be not only an alternative choice against traditional insulin for patients with insulin resistance or concurrent disease but also an effective home therapy medication in canine patients with DM. This study could help inform veterinary practitioners regarding the use of insulin detemir for canine insulin-dependent DM.

남성의 관점에서 본 노인 돌봄 경험과 역할전환에 관한 연구

최인희(In-Hee Choi),송효진(Hyo-Jean Song),지은숙(Eun-Sook Jee),정다은(Da-Eun Jung) 한국여성정책연구원 2016 한국여성정책연구원 연구보고서 Vol.2016 No.-

Ⅰ. 연구개요 1. 연구의 배경 및 목적 □ 최근 한국사회의 노인돌봄과 관련한 대표적인 변화는 ‘남성(남편, 아들 등)’이 독립적으로 일상생활을 영위하는데 제한이 있는 노인에게 돌봄을 제공하는 주된 역할을 수행하는 비율이 꾸준히 증가하고 있다는 점임. - 그러나, 기존 국내 노인돌봄 연구는 ‘여성’ 돌봄자의 돌봄 경험, 부양부담 및 부담경감 방안에 초점을 두고 수행되었으며, ‘돌봄의 주체’로서의 남성과 남성의 돌봄자로의 역할 전환(transition) 과정에 초점을 둔 연구는 제한적으로 수행됨. - 국외에서는 증가하는 남성돌봄자의 이슈를 탐색하기 위해 돌봄 수행방식 및 주돌봄자가 인지하는 노인돌봄 부담에 있어서의 성별차이(Akpinar et al., 2011; Montgomery, 1992; Pinquart & Sorensen, 2006; Stoller, 1992), 남성의 노인돌봄 경험(히라야마 료, 2015; Pretorius et al., 2009; Ribeiro et al., 2007; Sanders & Power, 2009) 및 남성성이 남성돌봄자들의 노인돌봄 경험에 미치는 영향(Baker et al., 2010; Russell, 2007) 등에 관한 다양한 연구가 진행된 바 있으며 국내에서도 노인을 돌보는 남성돌봄자에 대한 심층적 연구가 필요하다고 제안된 바 있으나(이순미·김혜경, 2009; 최희경, 2012a) 남성들의 돌봄 경험을 면밀히 탐색한 연구는 제한적으로 수행되었음. - 이에 본 연구는 남성들의 노인돌봄 경험을 남성의 관점에서 분석하고, 돌봄을 둘러싼 젠더정체성 및 가족관계 변화양상을 포착하여 선제적인 가족정책을 수립하는데 필요한 기초자료를 제공하고자 함. □ 본 연구의 목적은 남성(남편, 아들 등)에 의한 노인돌봄 현황 및 남성의 돌봄자로의 역할 전환 과정을 살펴보고, 이들의 정책지원 욕구를 파악하여 제도보완 및 남성 가족돌봄자를 위한 정책개발의 시사점을 제공하는 것임. 2. 연구의 주요 내용 □ 본 연구의 주요 연구문제는 다음과 같음. - 첫째, 배우자 또는 (조)부모를 돌보는 남성의 가족돌봄 현황(예: 돌봄기간, 시간, 돌봄 이유, 돌봄자 및 요보호노인의 건강상태, 사회적 지지망 등) 및 돌봄부담(burden)은 어떠한가? - 둘째, 남성이 배우자 또는 (조)부모를 돌보게 되면서 경험하게 된 변화(예: 관계적 측면, 돌봄의 의미 등)는 무엇인가? - 셋째, 배우자 또는 (조)부모를 돌보는 남성의 삶의 질은 어떠한가? - 넷째, 배우자 또는 (조)부모를 돌보는 남성의 가족돌봄 지원 정책(예: 노인장기요양보험제도 등)에 관한 인지도 및 이용 경험, 정책지원 욕구는 어떠한가? 3. 연구방법 □ 문헌 연구 - 본 연구에서는 남성의 노인 돌봄에 관한 국내외 선행연구 및 우리나라와 일본의 가족돌봄자 지원제도에 관한 자료를 검토하였음. □ 2차 통계자료 분석 - 2014년도 노인실태조사 (정경희 외, 2014)를 활용하여 요보호노인의 일상생활을 수행하는데 도움을 주는 사람, 가족원 중 가장 도움을 많이 준 사람 및 주당 돌봄시간 등을 분석하였음. □ 설문조사 및 분석 - 조사대상은 일상생활을 스스로 영위할 수 없는 55세 이상 노인을 조사시점에서 3개월 이상, 주당 15시간 이상 돌보고 있는 가족원 중 남성(남편, 아들 등) (N=247)으로 설정하였고, 구조화된 설문지를 이용하여 1:1 면접조사를 수행하였음. 조사표는 1) 가족 및 돌봄대상자 특성, 2) 돌봄현황, 3) 사회적 지지, 4) 응답자의 건강 및 경제활동, 5) 가치관 및 삶에 대한 태도, 6) 정책지원 욕구로 구성하였으며, 수집된 자료를 분석하여 남성의 돌봄 현황 및 돌봄 부담, 역할전환 과정, 돌봄지원 정책 및 서비스 이용 경험과 정책지원 욕구 등을 파악하였음. □ 심층면접 조사 및 분석 - 남성돌봄자의 돌봄 경험을 보다 입체적으로 파악하기 위해 일상생활을 독립적으로 영위할 수 없는 배우자 또는 부모를 조사시점에서 3개월 이상, 1주 평균 15시간 이상 돌보는 남성(17명)을 대상으로 1:1 면접을 수행하였음. 심층면접에서는 돌봄 상황 및 관계적 특성이 돌봄에 미치는 영향, 돌봄 경험의 의미, 돌봄지원 정책 및 서비스 이용 경험과 정책지원 욕구 등에 대해 살펴보았음. □ 전문가 자문회의 - 관련 분야 전문가를 대상으로 전문가 자문회의를 개최하여 연구설계 및 방법, 정책대안에 대한 관련 부처 및 전문가 자문의견을 수렴하였음. 4. 연구의 기대효과 및 한계점 □ 본 연구는 그동안 제한적으로 수행된 연구인 ‘남성’ 가족돌봄자의 노인돌봄 경험을 탐색하고, 학술적·정책적 시사점을 도출하였다는 점에서 연구의 의의를 찾을 수 있음. - 그러나, 노인을 돌보는 남성 가족돌봄자 모집단을 추정할 수 있는 자료의 부재로 임의할당추출에 의한 설문조사를 시행하여 결과를 일반화하는데 한계가 있음. - 남성돌봄자만을 대상으로 조사를 시행하여 여성돌봄자와의 돌봄 경험 및 부양부담의 차이를 직접적으로 비교하지 못 The number of male family caregivers in Korea, especially those providing spousal caregiving in old age, has been steadily increasing. However, since women have long predominated in the family caregiving role, few studies have explored the experience of caregiving by males in terms of the breadth and depth of care work and its meaning. The purpose of this study was to explore the caregiving experience of husbands and sons providing care to a spouse or parent(s) aged 55 and older who showed limitations in activities of daily living (ADL) or instrumental activities of daily living (IADL) for a period of at least six months. Both quantitative and qualitative methods were utilized for this study. Survey data were collected from a purposive sample of 247 male caregivers who served as the primary care providers for a dependent spouse or parent(s). Descriptive statistics and multivariate models were obtained using SPSS Statistics. In addition, semi-structured in-depth interviews with 17 male caregivers were conducted. For the qualitative analysis, a comprehensive content review of all data, including line-by-line analysis, was conducted. Informed consent was obtained from the participants. Some of the major findings are as follows: First of all, approximately one-fourth of respondents identified personal bonds as being their motivation for taking care of a dependent spouse or parent(s). The average caregiving period was around 47.7 months, and respondents spent approximately 34.1 hours per week on average on caregiving. Secondly, the majority of respondents in our sample reported that they experienced caregiving to be burdensome to some degree, especially in the areas of assistance with bathing (52.9%), voiding (49.5%), household management (44.6%), and meal preparation (50.6%). A significant number of respondents reported that one of the most difficult aspects of caregiving was balancing a personal life with the caregiving role, and most respondents stated that the initial phase of caregiving was the most difficult as they made the transition to the new role as caregiver and restructured their life. In addition, spousal caregivers generally experienced a higher level of caregiving burden compared to son caregivers since they are more likely to be older, unhealthier, and have fewer resources. However, a majority of male caregivers also found caregiving to be a very rewarding experience in that they felt useful (72.9%) by providing care to a dependent family member or they had grown closer to their dependent spouse or parent(s) as a result of the caring (54.7%). Thirdly, about 65% of respondents reported that they had secondary caregivers available to support them regularly in their caregiving. This finding supports previous research in which male caregivers were found to be more likely to have access to additional informal caregivers than were female caregivers. Fourth, although a significant number of respondents in our sample reported that they were aware of long-term care insurance (LTCI) benefits, the number of respondents who were receiving LTCI benefits was relatively smaller. Likewise, while approximately 80% of respondents reported being satisfied with the overall services available, the in-depth interview participants suggested that service adequacy, especially in-home care service, was insufficient for assisting family caregivers with balancing work life with the caregiving role. They also suggested that overall service quality should be improved to enhance quality of life among both older adults and family caregivers. However, in the regression analysis, use of LTCI benefits was not a predictor of male caregivers’ burdens.

부산시 고등학생들의 환경에 대한 인식정도와 영향요인 분석

최인영,윤성효,고정선 부산대학교 사범대학 과학교육연구소 2004 科學敎育硏究報 Vol.31 No.-

The purpose of this study was to identify the degree of recognition of the high school students about environmental and influential factors on it, selecting two regular schools and three vocational schools among the high schools in Busan area, questionnairing one class in the 10th, 11th, 12th grades of each school and analyzing the result. The result of analyzing the degree of knowledge, practice and recognition of seriousness concerning environment according to grade and gender is that the significant difference has been found in the degree of practice. The result of analyzing them according to maternal academic background is that the significant difference has been found in the degree of knowledge and a living standard and the style of housing have no effect on the environmental education, In the survey according to family style, it is shown that the students 'who live with their parents' recognize the seriousness of environment more. In the survey according to the experience of having the subject 'environment' in their school curriculum or not, it is shown that the significant difference has been found in the degree of knowledge. As the source of information on environment, mass media such as TV, magazines and newspapers have a great impact on the students. As the significant difference has been found in the degree of knowledge, practice, the recognition of seriousness from the viewpoint of the analysis on the survey according to the degree of the recognition on the necessity of the environmental preservation, it is shown that the more the students feel the necessity of environmental preservation, the higher the degree of knowledge, practice and recognition of seriousness on environment becomes, In the survey according to the kind of school, the significant difference has been found in the degree of knowledge, In the survey according to the degree of maternal concern on environment it is shown that the more concerns the mothers have, the more the students put what they learn into practice, In the survey according to the kinds of school, the significant difference has been found in the degree of knowledge. As the result of multiple regression analysis is summarized as below. 1. The variables influencing the environmental knowledge are the experience of having the class, 'environment', the recognition of the necessity of the environmental preservation and maternal academic background. 2. The elements influencing the degree of recognition of seriousness about environmental contamination are gender, the experience of having a class, 'environment' or not and the necessity of the environmental education. 3. The elements influencing the degree of practice are grade, information on environment, and maternal concern.

비소세포폐암에서 Microsatellite Instability

전효성,김정란,손지웅,박선하,박태인,김창호,김인산,정태훈,박재용 경북대학교 병원 2001 경북대학교병원의학연구소논문집 Vol.5 No.1

연구베경:MMR 유전자의 불활성화에 의해 야기되는 뮤전적 불안정성은 발암기전의 한 부류로 인정되고 있다.저자들은 비소세포폐암의 발암과정에서의 MSI의 역할을 규명하기 위해 비소세포폐암에서 MSI의 빈도 및 MSI 유무에 따른 임상상의 차이를 조사하였다. 대상 및 방법:근치적 절제술을 받은 비소세포폐암 20예를 대상으로 하였다.동결된 폐암조직과 환자의 림프구에서 DNA를 추출한 후 3P와 9p의 15개의 marker들을 대상으로 PCR을 시행하고 7% polyacrylamide gel에서 전기영동한 후 silver 염색을 시행하였다. 암조직과 림프구 DNA의 PCR product의 band를 비교하여 MSI와 LOH를 판정하였다. 결과:1)대상환자들은 남자 19예,여자 1예였으며 모두 흡연자였고 평균 흠연력은 47 갑년이었다. 폐암의 조직형은 편평상피암 15예, 선암 4예,대세포암 1예였고, 술 후 병리학적 병기는 Ⅰ기 6예,Ⅱ기 5예, ⅢA기 7예,ⅢB기 2예였다. 2)20예 가운데 8예(40%)에서 MSI가 관찰되었으며 3예는 한 개의 marker에서,5예는 2개 이상의 marker에서 MSI가 관찰되었다. 3)LOH는 10예(50%)에서 있었으며,LOH유무에 따른 병가 및 흡연력의 차이가 없었다. 4)분석한 marker의 10% 이상에서 MSI가 관찰된 MSI-L종양은 5예였으며, 대부분의 marker에서 MSI양성인 MSI-H종양은 없었다.MSS종양과 MSI-L종양은 흡연력, 병기, 폐암 조직형 및 LOH 빈도의 유의한 차이가 없었다. 결론:비소세포폐암에서 MSI는 비교적 흔히 관찰되지만 MMR 유전자의 불활성화에 의한 MMP pathway 는 비소세포폐암의 주요 발생기전은 아닐 것으로 생각된다.향 후 비소세포폐암의 발암과정에 있어서 MMP pathway의 역할을 규명하기 위해서는 보다 많은 예를 대상으로 한 연구가 필요할 것으로 생각되며, MSI 발생기전에 관한 추가적인 연구가 필요할 것으로 생각된다. Purpose: Microsatellite instability(MSI) is frequently used as an indicator of microsatellite mutator phenotype (MMP) tumors.MSI has been observed in a percentage of non-small cell lung cancer(NSCLC).However, its role in tumorigenesis of NSCLC remains unknown.The frequency and partten of MSI in NSCLC were evaluted and clinical parameters of MSI-positive tumors with those of MSS(microsatellite stable) tumors were compared. Materials and Methods: Twenty surgically resected NSCLCs were analyzed for 15 microsatellite markers located at chromosomes 3p and 9p.The peripheral blood lymphocytes of patients were used as the source of the normal DNA. Results:1) of 20 cases, 8(40%) demonstrated MSI. 2) Instability was observed more frequently in tri- and tetra-nucleotide repeats that in dinucleotide repeats. In all cases, instability appeared as a shift of individual allelic bands. 3) LOH was observed in 10(50%) of 20 tumors analyzed. 4) Of 20 cases, MSI-H tumor(showing MSI in the majority of markers) was absent. There were 5 MSI-L tumors( showing MSI in a greater than 10% of markers). 5) No significant difference was observed between MSI-L tumors and MSI negative tumors in terms of clinicopathologic features such as pack-year history of smoking, histologic subtype, and (delete) stage of disease.There was also no significant difference in the incidence of LOH in relation to the status of MSI. Conclusion: These data strongly suggest that MSI plays different roles in lung and colon cancer.MMP pathway appears to be far less important in the tumorigenesis of NSCLC, caused mainly by cigarette smoke, with little familial tendency.(tuberculosis and Respiratory Diseases 2000,48:24-32)

Adult invasive pneumococcal disease in the Republic of Korea: Risk medical conditions and mortality stratified by age group

Kim, Jong Hun,Baik, Seung Hee,Chun, Byung Chul,Song, Joon Young,Bae, In-Gyu,Kim, Hyo Youl,Kim, Dong-Min,Choi, Young Hwa,Choi, Won Suk,Jo, Yu Mi,Kwon, Hyun Hee,Jeong, Hye Won,Kim, Yeon-Sook,Kim, Jeong Elsevier 2018 INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES Vol.74 No.-

<P><B>Abstract</B></P> <P><B>Objectives</B></P> <P>This study aimed to characterize the risk factors for mortality in adult patients with invasive pneumococcal disease (IPD) stratified by age groups, after implementation of the national immunization program of 23-valent polysaccharide vaccine (PPSV23) for those aged ≥65 years in the Republic of Korea (ROK).</P> <P><B>Methods</B></P> <P>Clinical data and pneumococcal isolates from adult patients with IPD (≥18 years of age) were collected prospectively from 20 hospitals through the nationwide surveillance program from March 2013 to October 2015.</P> <P><B>Results</B></P> <P>A total of 319 patients with IPD were enrolled. Median age was 69 years. Overall in-hospital mortality was 34.2%: 17.1% in those aged 18–49 years, 23.7% in those aged 50–64 years, 33.0% in those aged 65–74 years, and 51.0% in those aged ≥75 years (<I>p<</I> 0.001). In particular, early death within 7days of hospitalization accounted for 60.6% (66/109). While old age (≥65 years), higher Pitt bacteremia score (≥4), and bacteremic pneumonia were independently associated with IPD mortality in all age groups, an additional mortality risk factor of immunocompromised status was identified for patients aged 50–64 years. PPSV23 serotypes accounted for 64.4% (122/189) of the pneumococcal isolates serotyped.</P> <P><B>Conclusions</B></P> <P>This study suggests that vaccine-type IPD continues to place a substantial burden on older adults in the ROK, necessitating an effective vaccination strategy for those at higher risk.</P> <P><B>Highlights</B></P> <P> <UL> <LI> National immunization of the elderly with the 23-valent polysaccharide vaccine (PPSV23) was implemented in Korea in 2013. </LI> <LI> Overall in-hospital mortality was 34.2% for adult invasive pneumococcal disease (IPD) and 51.0% for patients ≥75 years of age. </LI> <LI> Mortality risks were older age, higher bacteremia score, and immunocompromised status. </LI> <LI> An effective vaccination strategy for those at higher risk of IPD is needed. </LI> </UL> </P>

위암종과 Epstein-Barr 바이러스와의 연관성에 대한 연구

심광용,김호영,김효열,백순구,권상옥,조미연,이창훈,이종인 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Objectives: Epstein-Barr virus (EBV) has been known to be linked to a spectrum of neoplastic conditions, including nasopharyngeal carcinoma, Burkitt's lymphoma, peripheral T-cell lymphoma and Hodgkin's disease. This study aims to investigate the association of EBV with gastric carcinoma in Korea. Methods: Fifty-three cases of gastric adenocarcinoma were studied for evidence of EBV infection by EBV-encoded small RNA (EBER) in situ hybridization (ISH) on the paraffin sections and amplifying the EBV genome encoding envelop glycoprotein (gp220) with polymerase chain reaction (PCR) in fresh gastric cancer specimens. Results: EBER was detected in 7 (13.2%) of 53 gastric carcinomas and all cases were male. In 6 (85.7%) of 7 EBER-positive cases, the tumors were located in the upper and middle part of the stomach. EBER ISH study showed strong positivity in all the tumor cells, but negativity in surrouning lymphocytes, stromal cells and normal gastric mucosa. DNA PCR was positive in 23 (43.4%) of 53 gastric carcinomas, including all of 7 EBER-positive cases. Conclusion: We could observe some association of EBV with gastric carcinoma, and our findings about the charateristics of EBV-positive gastric cancers involving sex, site and histological type are similar to those in Japan. However, EBV-positive rate of gastric cancer is higher than in Japan and lower than in Western countries. Further studies to elucidate oncogenic mechanism of EBV in gastric cancer should be performed.

오차드그래스(Dactylis glomerata L.)의 품종에 따른 종자유래의 캘러스 형성률과, 캘러스 크기 및 식물체 재분화 효율의 비교

배은경,이인애,김기용,이병현,손대영,이효신,정민섭,조진기 Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-

Comparison results of callus formation ratio from seed explants, callus sizes, regeneration ratios from callus and regeneration efficiency [calculated by following formular; callus formation ratio(%) × regeneration ratio(%)/100]for 27 ochardgrass (Dactylis glomerata L.)varieties imported and Hapsung 2 developed in Korea are as follows; 1. Among ochardgrass varieties showing more than 50% callus formation ratios, the descending order of callus formation ratio after bedding the seed explants for 4 weeks was 93>Sparta>Pizza>Condor>Lidaglo>Glorus>Hapsung2>Frode. 2. The callus sizes after bedding for 4 weeks were in the range of φ 0.43cm~4.2cm in which there was 10 times size difference between the largest one and the smallest one but most of them were between φ2.5cm~4cm. 3. The regeneration ratio from callus among varieties were in the range of 0~36% and descending order of the upper 6 varieties was Plano>Akimidori>Justus>Lidacta>Currie>Hall mark. 4. The regeneration efficiency which is calculated by the ratios of regeneration from seed explant numbers was between 0 to 17.4% among which Justus showed the highest value in the 4-week treatment. 5. The correlation between callus formation ratios and the callus sizes, callus formation ratios and regeneration efficiency, and callus sizes and regeneration efficiency were r=0.5765, r=0.6365 and r=0.6246, respectively in 4-week callus and all the correlations were significant on the 1% level. 6. In 6-week callus, the descending order callus formation ratios from seed sxplants for the best 6 varieties was Condor>Sparta>93M>Justus>Potomac>Lidaglo>Frode. 7. The callus sizes formed were between φ1.5~5.7cm in which Sparta, the largest one of φ5.7cm was five times larger than the smallest one. The callus size of the control variety. Hapsung 2 was φ3.8cm, which belonged to larger size. 8. Regeneration ratio showed a great deviation among varieties from 6-week old calli by showing from 0% to 100% in which all the calli were regenerated in Plano while no callus was regenerated in Juno, 9. The range of regeneration effciency was between 0~28% among varieties in which the values from 6-week callus treatment were larger than those from 4-week callus treatment. Especially, the value of Potomac in 6-week was 3 times larger than that in 4-week. 10. The correlation between callus formation ratios and the callus sizes, callus formation ratios and regeneration efficiency, and callus sizes and regeneration effciency were r=0.8369, r=0.6683 and r=0.5937, respectively in 6-week callus, and all the correlations were significant on the 1% level.

돈지, 들깨유 및 달맞이꽃 종자유의 혼합급이가 흰쥐의 간장, 뇌 및 고환의 지질 성분에 미치는 영향

김성희,김한수,서인숙,정효숙,정승용 慶尙大學校 1992 論文集 Vol.31 No.2

돈지 및 들깨유와 달맞이꽃 종자유등 2종 식물 종자유와의 혼합급이가 흰쥐의 체내 조직의 지질 성분에 미치는 영향을 구명하기 위해 S·D계 숫쥐에게 돈지 2.55및 들깨유와 달맞이꽃 종자유의 혼합비율을 달리하여 4주간 급이 사육한 후 간장,뇌 및 고환의 지질 성분을 분석 검토한 결과는 다음과 같다. 간장의 총콜레스테롤 함량은 D군이 대조군에 비해 유의적으로 낮았으며, 인지질 함량은 각 군간의 유의적인 차이가 없었고, 중성지질 함량은 B및 C군이 대조군에 비해 현저하게 낮았다. 콜레스테롤 급이군의 간장 총콜레스테롤 함량은 대조군에 비해 c,d및 e군이 유의적으로 낮았고, 인지질 및 중성지질의 함량은 b군이 낮게 나타났다. 뇌의 총콜레스테롤 함량은 대조군에 비해 B및 C군이 유의적으로 낮았으며, 인지질 및 중성지질의 함량은 대조군에 비해 전 군이 유의적으로 낮데 나타났다. 콜레스테롤 급이군에 있어 뇌의 총콜레스테롤 함량은 각 군간의 유의적인 차이가 없었고, 인지질 함량은 c군이 중성지질 함량은 e군이 대조군에 비해 낮게 나타났다. 고환의 총콜레스테롤 및 인지질 함량은 각 군간의 유의적인 차이가 없었으나, 중성지질 함량은 대조군에 비해 전 군이 유의적으로 낮았다. 콜레스테롤 급이군은 콜레스테롤 무급이군과 거의 유사한 경향을 나타내었다. In order to observe the effects of the feeds mixed with the lard and two vegetable seed oils, which mixed with 2.5% lard and various level of perilla oil and evening primrose oil were administered to the male rats of Sprague Dawley for 4 weeks on lipid components of liver, brain and testes. In livers , total cholesterol contents were remarkably lower in the D group(2.5% lard+2.5% perilla oil+5.0% evening primrose oil,free cholesterol) than in the control group , and phospholipid contents were not significant difference in each groups. Triglyceride contents were remarkably lower in the B(2.5% lard+7.5% perilla oil+5.0% ,free cholesterol) and C(2.5% lard+5.0% perilla oil+2.5% evening primrose oil,free cholesterol) group than in the control group. In the supplemented cholesterol groups, total cholesterol contents were significantly lower in c(C group+cholesterol diet), d(D group+cholesterol diet) and e(2.5% lard+7.5% evening primrose oil,free cholesterol) group, phhspholipid and triglyceride contents were lower in the b group(B group+cholesterol diet) than in the control groups than in the control group, phospholipid and triglyceride contents in brain were considerably lower in the other groups than in the control group. In the supplemented cholesterol groups, total cholesterol contents were not significant difference in each groups. Phospholipid and triglyceride contents were conspicuously lower in the c and e groups. respectively. In testes, total cholesterol and phospholipid contents were not significant difference in the groups, but triglyceride contents were lower in the all groups except for control group The contents of total cholesterlo, phospholipid and triglyceride were similar between the supplimented cholesterol groups and the nonsupplemented cholesterol groups.

Clinical impact of admission hyperglycemia on in-hospital mortality in acute myocardial infarction patients

Kim, Eun Jung,Jeong, Myung Ho,Kim, Ju Han,Ahn, Tae Hoon,Seung, Ki Bae,Oh, Dong Joo,Kim, Hyo-Soo,Gwon, Hyeon Cheol,Seong, In Whan,Hwang, Kyung Kuk,Chae, Shung Chull,Kim, Kwon-Bae,Kim, Young Jo,Cha, Kwa Elsevier 2017 INTERNATIONAL JOURNAL OF CARDIOLOGY Vol.236 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Acute hyperglycemia on admission is common in acute myocardial infarction (AMI) patients regardless of diabetic status, and is known as one of prognostic factors. However, the effect of hyperglycemia on non-diabetic patients is still on debate.</P> <P><B>Methods</B></P> <P>A total of 12,625 AMI patients (64.0±12.6years, 26.1% female) who were enrolled in Korea Acute Myocardial Infarction Registry-National Institute of Health between November 2011 and December 2015, were classified into 4367 diabetes (65.4±11.6years, 30.4% female) and 8228 non-diabetes (63.3±13years, 23.9% female). Patients were analyzed for in-hospital clinical outcome according to admission hyperglycemic status.</P> <P><B>Results</B></P> <P>In diabetic patients, independent predictors of in-hospital mortality were old age, high HbA<SUB>1</SUB>C, pre-Thrombolysis In Myocardial Infarction (TIMI) flow 0, left ventricle ejection fraction<40%, cardiogenic shock and ventricular tachycardia. In non-diabetic patients, independent predictors of in-hospital mortality were old age, high admission glucose (≥200mg/dL), pre TIMI flow 0, failed percutaneous coronary intervention, low left ventricle ejection fraction<40%, cardiogenic shock, stent thrombosis and decreased Hb≥5g/dL. In hospital mortality was significantly higher in diabetic patients compared to non-diabetic patients (5.0% vs. 3.4%, <I>p</I> <0.001). However, non-diabetic patients with hyperglycemia have significantly higher mortality compared to diabetic patients (17.4% vs. 7.2%, <I>p</I> <0.001). Comorbidity including cardiogenic shock (<I>p</I> <0.001), cerebral hemorrhage (<I>p</I> =0.012), decreased Hb≥5g/dL (<I>p</I> =0.013), atrioventricular block (<I>p</I> <0.001) and ventricular tachycardia (<I>p</I> =0.007) was higher in non-diabetic with hyperglycemia than in diabetic patients.</P> <P><B>Conclusions</B></P> <P>These findings underscore clinical significance of admission hyperglycemia on in-hospital mortality in non-diabetic AMI patients.</P>