Duplication or deletion of chromosome 17p11.2-p12 in CMT1 and HNPP peripheral neuropathy patients

Kim, Hyo-Sun,Jang, Young-Eun,Chung, Ki-Wha 公州大學校 基礎科學硏究所 2003 自然科學硏究 Vol.10 No.-

CMT와 HNPP 말초신경증은 가장 흔하게 발병하는 운동 및 감각 말초신경계의 선천성 질환이다. CMT1과 HNPP의 유전적 원인으로는 염색체 17p11.2-p12의 1.5 Mb 지역의 중복과 결실이 각각 50%이상과 70% 이상을 차지하는 것으로 알려졌다. 본 연구에서 한국인 CMT1 및 HNPP 환자를 대상으로 중복 및 결실을 조사하였는데, 31 CMT1 가족 중 13 가족에서 중복을 보였으며 (42%), 24 HNPP 가족 중 19 가족에서 결실을 보였다 (79%). 17p11.2-p12 중복을 보이는 CMT1A 환자가 HNPP 결실환자보다 발병 연령, 발병 기간, 근육 이양성 및 족 기형성에서 훨씬 심한 증상을 보였다. Charcot-Marie-Tooth disease (CMT) and hereditary neuropathy with liability to pressure palsies (HNPP) are most common inherited motor and sensory peripheral neuropathies. The majority of CMT1 patients have a 1.5 Mb duplication in chromosome 17p11.2 while most HNPP patients have a deletion of the same region. In the analysis of Korean CMT1 and HNPP patients, each HNPP deletion and CMT1A duplication was observed from 79% (19 families) among 24 HNPP families and 42% (13 families) among 31 CMT1 families. CMT1A duplication patients showed more severe clinical symptoms than HNPP deletion patients, when we examined oneset age, disease duration, muscular atrophy, foot deformity and scoliosis.

청국장에 관한 연구(Ⅰ) : 청국장 제조과정에 있어서 콩단백질의 변화에 대하여 On the changes of soy - bean protein in manufacturing Chung - Kook - Jang

이계호,이효지,정문교 한국농화학회 1971 Applied Biological Chemistry (Appl Biol Chem) Vol.14 No.3

As a series on the soy-bean protein and their related substances 9 samples were collected from 9 places such as straws (Rice) to obtain bacterial strains which produce protease. From these samples total of 23 strains were isolated by the use of dilution pour plate method. For all isolated strains primary screening of productivity of protease was performed and useful straines with regard to protease productivities were identified. Optimum conditions for enzyme action of protease from isolates D_9, F_(20) strains were pH 7.5 and 40℃. Chung-Kook-Jang is one of the characteristic foods in Korea made from soy-bean by fermentation. The chief bacterium is Bacillus subtilis and the chief change which takes place in soy-bean during fermentation is degradation of protein. Three kinds of Chung-Kook-Jang were prepared using three different strains of Bacillus natto, D_9 and F_(20) from isolated. Water soluble-N, TCA soluble-N, amino-N and peptide-N were measured about the steamed soybean, Chung-Kook-Jang prepared with three strains of bacteria. Water soluble-N decreased very largely in steamed soybean, but in Chung-Kook-Jang it increased to 85% of raw soy-bean.

Environmental Microbiology / Microbial Diversity : Calcium Carbonate Precipitation by Bacillus and Sporosarcina Strains Isolated from Concrete and Analysis of the Bacterial Community of Concrete(s)

( Hyun Jung Kim ),( Hyo Jung Eom ),( Chulwoo Park ),( Jaejoon Jung ),( Bora Shin ),( Wook Kim ),( Namhyun Chung ),( In Geol Choi ),( Woojun Park ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.3

Microbially induced calcium carbonate precipitation (CCP) is a long-standing but re-emerging environmental engineering process for production of self-healing concrete, bioremediation, and long-term storage of CO2. CCP-capable bacteria, two Bacillus strains (JH3 and JH7) and one Sporosarcina strain (HYO08), were isolated from two samples of concrete and characterized phylogenetically. Calcium carbonate crystals precipitated by the three strains were morphologically distinct according to field emission scanning electron microscopy. Energy dispersive X-ray spectrometry mapping confirmed biomineralization via extracellular calcium carbonate production. The three strains differed in their physiological characteristics: growth at alkali pH and high NaCl concentrations, and urease activity. Sporosarcina sp. HYO08 and Bacillus sp. JH7 were more alkali- and halotolerant, respectively. Analysis of the community from the same concrete samples using barcoded pyrosequencing revealed that the relative abundance of Bacillus and Sporosarcina species was low, which indicated low culturability of other dominant bacteria. This study suggests that calcium carbonate crystals with different properties can be produced by various CCP-capable strains, and other novel isolates await discovery.

장대형 사각공간에서의 가열원이 난류 자연대류에 미친는 영향

정한식,정효민 慶尙大學校 1998 論文集 Vol.37 No.-

The heat sources effect on turbulent natural convection in large longitudinal rectangular space has been investigated using a finite volume method. The results of the isotherms and velocity vectors have been represented for various heating capacities at the 8 heat sources. At upper position in the space, the temperature and velocity are represented higher value. The mean temperature of the space can be expressed as function with the heating capacity.

위 유문동 평활근 세포에서 Caffeine에 의해 활성화되는 이온 전류에 관한 연구

정인원,이무열,방효원,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1992 中央醫大誌 Vol.17 No.3

In order to clarify the existence of the non-selective cationic channel of CI^- -channel in gastric antral smooth muscle cells which suggested to play a critical role in generation and maintenance of depolarization and exitability, the whole cell mode of patch-clamp experiment was conducted. Following results were obtained ; 1. Puff injection of caffeine induced the increase of inward currents in the hyperpolarized holding potential ranges, and the increase of outward currents in the hypopolarized range. 2. In the case of blocking of all known currents, these current components were observed. 3. These current components were disappeared when known as a Cl^- -channel blocking agent was treated and intracellular EGTA concentration was increased. The above results could be concluded that these current components strongly suggested as a Ca^2+ -dependent Cl^- current in the gastric antral smooth muscle cells.

한국산 뱀독이 개구리 심장심실 근육 세포의 활동전압에 미치는 영향

정해성,방효원,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1984 中央醫大誌 Vol.9 No.2

Action potential of a frog ventricular cell was studied by conventional 3M KCI glass microelectrodes to investigate the effect of freeze-dried Korean snake venom. Following results were obtained; 1. Time to repolarize 50% of its action potential increased from 756±26mS to 796±17mS. 2. The notch which would be seen after the fast depolarization disappeared and the overshoot potential significantly decreased from 24.4±0.9㎷ to 17.8±2.3㎷. 3. Positive depolarization rate (dV/dt max) decreased from 22.8±0.8V/s to 18.8±0.8V/s. 4. Resting membrane potential was -95±3.0㎷ and showed little change. Above results would be interpreted that the Korean snake venom blocked the fast sodium channel and slow calcium channel simultaneously to reduce the contractility and activation of potassium channel which could result the prolongation of action potential duration.

뇨(尿)중 3-Methylhistidine 함량의 측정과 이용

정수현,서형주,김윤숙,이효구,강덕호 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.2

기존의 방법을 일부 수정하여 뇨중 3-methylhistidine을 분석하였다. 뇨중 3-methylhistidine을 fluorescamine 유도체화하여 HPLC에 주입하고 C_18 column과 10 mM acetonitrile/sodium phosphate buffer(pH 7.5)로 분리·용출시켜 형광검출기로 측정하였다. 3-methylhistidine의 체류시간은 7분 이내이었으며, histidine과의 분리상태도 서로 간섭함이 없이 양호하였다. 뇨에 3-methylhistidine을 첨가하고 이를 분석하였을 때의 회수율은 93∼106%로 높은 수준이었다. 체육학과 남학생중 웨이트 트레이닝 단련자와 비단련자를 대상으로 조사한 단기간의 웨이트 트레이닝에 따른 뇨중 3-methylhistidine 함량의 변화는 두 집단 모두 웨이트 트레이닝후의 3-methylhistidine 분비량이 유의하게 증가하였다. A modified method is given for the precolumn derivatization and subsequent high-pressure liquid chromatographic seperation of 3-methylhistidine from urine. The elution contained isocratic solution with acetonirile and 10 mM sodium phosphate(pH 7.5) requires less than 7 min. The recoveries of 3-methylhistidine from urine control were 93% to 106%. 3-Methylhistidine determinations were performed on urine samples from volunteers who were both male trained and non-trained physical undergraduates. As the result, urinary 3-methylhistidine content of volunteers increased significantly after weight training.

기단추적에 의한 대기혼탁도 변화

정효상,조희구 연세대학교 자연과학연구소 1985 學術論文集 Vol.14 No.-

은반일사계로 관측된 서울 (1969~1982), 부산및 광주(1980~1982)의 직달일사량을 사용하여 에어로솔만에 의한 대기혼탁도를 Yamamoto법으로 계산하였다. 각관측소에서 관측된 대기혼탁도는 기단의 발원지, 이동경로및 이동시간과 각 관측소의 국지기상상태에 의해서 영향을 받고 있었다. 서울에서 대기혼탁도는 연중 시정에 반비례, 습도에 비례하였다. 그러나 봄, 가을 그리고 겨울에는 풍속에 반비례 하나 여름에는 관계가 뚜렷하지 않았다. 서울의 대기혼탁도는 매년 증가추세를 보이고 있으며, 일중 정오(1200LST)의 혼탁도가 가장 높았다. The atmospheric turbidity was calculated by Yamamoto's method (1968), using direct solar measurements in Seoul from 1969 to 1982, Busan and Kwangju from 1980 to 1982, and simultaneous radiosonde observations at Osan. Air mass trajectories in the vicinity of Korea were used to study the dependence of atmospheric turbidity on the origin and route of air mass and its travelling time. The variations of atmospheric turbidity for wind, visibility and humidity were investigated only in Seoul. The air mass trajectories drawn represented that the air mass moved from the northern or southern China might change its quality after it approached near Korean peninsula and during that period, the atmospheric turbidity increased gradually. The seasonal value of turbidity in Seoul appeared to be slightly dependent inversely on the surface wind speed with high values for south-easterly wind aside from Summer. The daily turbidity maximum occurred at noon in Seoul.

수영 인구의 저변확대를 위한 활성화 방안에 관한 연구

정명수,임효택,손배민 조선대학교 스포츠과학연구소 1999 스포츠科學硏究 Vol.10 No.-

The purposes of this study are to examine characteristics of participants in leasure and sport activities and to explore ways for an increase in swimming population. 850 questionnaires were distributed to 850 adults who had participated in leasure and sport activities at 7 commercial swimming pools in Kwangju. Of these, 813 questionnaires were collected and 790 questionnaires were used for this study. The results were as follows. 1. Most participants in swimming did exercise for health and physical strengthen improvement. Above 1.3-1.6 million won monthly payed-people more participated in swimming than other people. 2. Many people participated in swim between 9 and 12 AM and more than 3-4 times a week. Usually, they went to swimming pools by their own cars and most of them took lesson in swimming. 3. Most sports facility users felt that utility fee was moderate and resting rooms and sports equipment shops were satisfactory, but other subsidiary facilities were not satisfactory dissatisfaction with swimming pool facilities and service of employees revered to be the most barrier to access to swimming pools. Thus, program development by step by step approach for beginners and active investment for convenience facilities might be necessary. And also more active governmental support are needed and public facilities should be established and opened for more people to access more easily to sports facilities.

어린이집 형태로 운영하는 예체능 학원의 운영실태와 학부모의 인식에 관한 연구 : 대구·경북지방을 중심으로

정정희,김수영,최효정 한국영유아보육학회 2003 한국영유아보육학 Vol.0 No.35

Increasing numbers of children are being served in more formal, academic instructional institution. The purpose of this study was to investigate the phenomena of early private education for preschool children at private institution. The subjects of this study were 94 directors, 88 teachers, and 197 parents who were selected by random sample from private institution in Taegu and Kyungpook area. Major findings were as follows: (1) 88% of institutes had no playground and their classrooms were placed on the second or third floors of the buildings. (2) Most of the institutes had educational objectives, but the goal content was not balanced, especially social-emotional development was over-stressed in comparison with other educational objectives. (3) Many parents were shown to have high and unrealistic expectations regarding achievement of preschool children. The parents' main reason for having their children early private educations were helping children for their academic achievements.