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RISS 인기검색어
- 검색키워드
- 저자 : Hyeung-SikLee (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
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An immunohistochemical Study on the Pancreatic Endocrine Cells of the C57Bl/6 Mouse
Hyeung-Sik Lee,Hyeung-SikLee,Sae-KywangKu 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.4
The regional distribution and relative frequency ofthe pancreatic endocrine cells in the C57BL/6 mousewere studied by immunohistochemical method usingfour types of specific mammalian antisera againstinsulin,glucagon,somatostatin and human pancreaticpolypeptide (PP). The pancreas of mouse could bedivided into three portions;pancreatic islets, pancreaticduct and exocrine portions, and pancreatic isletswere further subdivided into three regions (central,mantle and peripheral regions) according to theirlocated types of immunoreactive cells and pancreaticduct portions were also subdivided into two regions(epithelial and connective tissue regions). In thepancreatic islet portions, although some cells werealso demonstrated in the mantle regions, most ofinsulin-immunoreactive cells were located in thecentral regions and they were randomly dispersed inthe whole pancreatic islets.Glucagon-immunoreactivecells were detected in the mantle and peripheralregions. Their relative frequencies in the peripheralregions were somewhat numerous than those of themantle regions. Somatostatin-immunoreactive cellswere detected in the mantle and peripheral regions.However,no PP-immunoreactive cells were demonstratedin the pancreatic islets of C57BL/6 mouse. In thepancreatic duct portions,rare glucagon-immunoreactivecells were situated in the epithelial regions. Cellclusters that consisted of glucagon- or somatostatin-immunoreactive cells were found in some case ofconnective tissue regions of pancreatic ducts.However,insulin-and PP-immunoreactive cells were not detectedin the epithelial nor connective tissue regions.In theexocrine portions, all four types of immunoreactivecells except for PP cells were demonstrated in the*Corresponding author: Hyeung-Sik Lee
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Jae-Hyun Lee,Hyeung-SikLee 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.3
The regional distribution and relative frequency ofinsulin-, glucagon-, somatostatin- and pancreaticpolypeptide (PP)-producing endocrine cels in thepancreas of BALB/c mouse were investigated byimunohistochemical method. The pancreas of micewas divided into two portions; pancreatic islets andexocrine portions, and pancreatic islets were furthersubdivided into two regions (central and peripheralregions) and the relative frequency and regionalglucagon, somatostatin and P antisera were monitored.In the pancreatic islet portions, insulin-imunoreactivecels were located in the central regions and theywere randomly dispersed in the whole pancreaticislets in some case of the smal islets. Quite diferentfrom those of other mammals, glucagon-imunoreactivecells were dispersed throughout central to peripheralregions in case of large islets and in the smaller ones,most of these cells were situated in the peripheralregions. Somatostatin-imunoreactive cels were detectedin the peripheral regions with various frequencies.regions of pancreatic islets, most of P-imunoreactivecels were located in the peripheral regions. In theexocrine portions, al four types of imunoreactivecells were demonstrated in the BALB/c mouse. Somepeculiar distributional paterns of pancreatic endocrinecells were found in BALB/c mouse, especially in caseof glucagon-imunoreactive cels.
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