Inhibition of oxidative stress induced‑cytotoxicity by coptisine in V79‑4 Chinese hamster lung fibroblasts through the induction of Nrf‑2 mediated HO‑1 expression

Hyeon‑Gyun Jo,Cheol Park,이혜숙,Gi‑Young Kim,Young‑Sam Keum,Jin Won Hyun,Taeg Kyu Kwon,Yung Hyun Choi,Su Hyun Hong 한국유전학회 2021 Genes & Genomics Vol.43 No.1

Background Coptisine is a natural alkaloid compound and is known to have multiple benefcial efects including antioxidant activity. However, whether it can protect lung fbroblasts from oxidative damage has not been studied yet. Objectives To investigate the potential inhibitory efect of coptisine against oxidative stress in V79-4 lung fbroblast cells. Methods V79-4 cells were treated with H2O2 (1 mM) in the presence or absence of coptisine (50 µg/ml), N-acetyl cysteine (NAC, 10 mM) or zinc protoporphyrin IX (ZnPP, 10 µM) for the indicated times. The alleviating efects of coptisine on cytotoxicity, cell cycle arrest, apoptosis, reactive oxygen species (ROS) production, DNA damage, mitochondrial dynamics, and inhibition of ATP production against H2O2 were investigated. Western blot analysis was used to analyze the expression levels of specifc proteins. Results Coptisine inhibited H2O2-induced cytotoxicity and DNA damage by blocking abnormal ROS generation. H2O2 treatment caused cell cycle arrest at the G2/M phase accompanied by increased expression of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP1 and decreased expression of cyclin B1 and cyclin A. However, these efects were attenuated in the presence of coptisine or NAC. Coptisine also prevented apoptosis by decreasing the rate of Bax/Bcl-2 expression in H2O2-stimulated cells and suppressing the loss of mitochondrial membrane potential and the cytosolic release of cytochrome c. In addition, the activation of nuclear factor-erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was markedly promoted by coptisine in the presence of H2O2. However, zinc protoporphyrin IX, a potent inhibitor of HO-1, attenuated the ROS scavenging and anti-apoptotic efects of coptisine. Conclusions Based on current data, we suggest that coptisine can be used as a potential treatment for oxidative stress-related lung disease.

저소득층 취업모 자기보호 아동의 사회적 능력 , 또래관계 및 정서발달

최경순(Kyung Soon Choi),정현희(Hyeon Hee Jeong) 한국아동학회 1996 兒童學會誌 Vol.17 No.2

This study investigated social competence, peer contacts, and emotional development in children of lower SES employed mothers. Instruments were Choi`s (1992) questionnaire on social competence, Pettit`s (1988) questionnaire on peer contacts, and Kang`s (1986) and Kim`s (1993) questionnaires on emotional development. The data was analyzed by t-test. Social class differences were found in children`s social capability, leadership and anxiety, in peer contacts, and in emotional disturbance and aggression.

노후 공동주택의 개조실태 및 개조요구에 따른 주호평면계획에 관한 연구

최준영,권현호 啓明專門大學 産業開發硏究所 2002 啓明硏究論叢 Vol.20 No.1

본 논문은 노후 아파트 거주자의 개조실태 및 요구사항을 조사·분석하여 주생활변화에 따른 다양한 주요구 변화에 대응할 수 있는 내부평면계획을 위한 기초자료를 마련하는데 그 목적이 있다. 결과는 다음과 같다. 1) 개조행위는 주부의 연령이나 가구의 월 소득수준. 가족생활주기. 거주년수에 따라 개조내용에 상당한 차이가 나타났다. 2) 내부공간적 측면에서의 개조요구는 가사작업공간>사적공간>공적공간>위생공간>서비스 공간순으로 나타났다. 더욱이 작업에 따른 불편, 가족생활주기, 생활변화에 따른 공간조절 가족취향에 맞는 분위기, 수납공간 부족과 같은 이유로 3가지 개조요구 및 4가지의 평면유형으로 분류되었다. 3) 구조적 측면에서는 공간의 변화와 확장을 위해 기존의 내력벽 구조에 독립기둥, 조적조, 가변형 벽체의 절충식 구조방식이 고려되어야 할 것이다. This study aims to provide basic data that investigate and analyze to actual condition and dwellers needs of repair about deteriorated multi-family hosing, planning of interior which correspond with various a dwelling needs according to change of housing life. The results were as follows: ⅰ) The repair behaviors showed differences in according to the wives age, monthly income, family life cycle and dwelling years ⅱ) In the aspect of interior space, the needs of repair appearance in the order of household space>private space>public space>sanitary space>service space. In addition, the contents and patterns of repair were each classified into three and four. ⅲ) In the aspect of structure, it should be consider eclectic structure with independent pillar, masonry structure to be variable wall for change and expand of space.

DBME(Discipline-Based Music Education)에 의한 음악과 교육과정의 비판적 분석과 발전 방향 탐색

최윤경,강현석 이화여자대학교 사범대학 교육과학연구소 2005 교육과학연구 Vol.36 No.2

오늘날 음악 교육은 입시 위주의 교육 정책, 국가 경쟁력의 강화라는 표어 아래에서 학교 당국과 교사, 학생, 학부모에게 부담스러운 과목으로 외면당하고 있으며, 음악 교육의 가치와 당위성마저 흔들리는 위기에 직면해 있다. 이러한 위기는 현재까지의 음악 교육이 일반적으로 ‘음악 이론과 기능을 가르치고 음악 활동을 하는 교과’로 인식되어 왔고, 음악과 교육과정 역시 ‘이해해야 할 지식’과 ‘경험해야 할 행위와 활동’을 단순히 나열하고 있었기 때문이라고 할 수 있다. 우리나라와 비슷한 위기에 직면했던 미국은 예술 교육의 정상화를 위해 미술교육에서 DBAE(Discipline-Based Art Education) 이론을 개발하였고, DBAE의 연구 결과에 기초하여 음악 교육의 문제를 해결하고자 연구된 것이 DBME(Discipline-Based Music Education)이다. 이에 본 연구는 DBME의 역사적 배경, 목적과 내용, 특징을 살펴보고, 이를 통해 현행 음악과 교육과정을 비판적으로 분석하였으며, 향후 음악과 교육과정이 ‘사회적?역사적?문화적 맥락을 고려’하고, ‘사고와 탐구 활동을 강조’하고, ‘디지털 중심의 사회적 변화를 수용’하는 음악과 교육과정으로 발전하여야 함을 제안하였다. Today, music education is a burden to teachers, students and parents and music is recognized to difficult subject. The reason is why the purpose of education changes into the university entrance and the policy of economic growth has precedence. So, The value of music education have faced at risk. The reason is that music education was recognized by subject matter that teaches music theory and function and does music activity until now. And music curriculum lists knowledge to understand, and activity to experience. The United States of America that faced at risk similar our country developed DBAE(Discipline-Based Art Education) theory in art education to solve problem of art education. DBME(Discipline-Based Music Education) is based on DBAE and studied to solve problem of music education. DBME involves music-making, musical history, musical aesthetics and musical criticism. Each of these disciplines are related and integrated to provide students with a balance understanding of works of music. This study examines historical background, purpose, contents, characteristic of DBME for theoretical explanation. Through this, current music curriculum is analyzed critically. And the direction of music curriculum is presented as following. So Music curriculum that accommodates engineering, considers social, historical, cultural context, and emphasizes thinking ability is emphasized in the next music curriculum revision.

초등과학영재학급 학생의 학습양식과 과학탐구능력 간의 상관관계

최선영,송현정,강호감 한국초등과학교육학회 2005 초등과학교육 Vol.24 No.2

The purpose of this study was to investigate the relation between the learning styles and science process skills of students of the gifted class in elementary school. Subjects were forty-eight students of the gifted class who are in the fifth grade studying at the gifted class of S elementary school in Bucheon, M and Y elementary school in Incheon on learning styles and science process skills of students. Learning Style Profile (LSP) was used as instrument to survey learning style of students of the gifted class which was developed by NASSP, and consists of four categories (cognitive skills, perceptual response, orientation and learning preferences) and twenty-four subscales. The results of this study were as follows: 1. In the learning styles test, students of the gifted class have higher scores of spatial skill, sequential processing skill, persistence orientation, manipulative preference, temperature preference and after-noon preference than general class students, but they have lower scores of discrimination skill and lighting preference, and there were statistically significant difference. 2. In science process skills test, there were statistically significant difference between students of the gifted class and general students. 3. In the correlation between the learning styles and science process skills, there was positive correlation of observing skill with spatial skill and manipulate skill of cognitive skill domain. For classifying skill, there was positive correlation with visual perceptual response, but was negative correlations with auditory and emotive perceptual response of perceptual response domain and with evening preference and verbal risk orientation of study preference domain. For measuring skill, there was positive correlation with sequential processing skill of cognitive skill domain. For formulating hypotheses, there was positive correlation with persistence orientation of study preference domain. For controlling variables, there was positive correlation with sequential processing skill and simultaneous processing skill of cognitive skill domain, and with verbal-spatial preference and early morning study preference of study preference domain. When planning and man-aging the gifted class, it will be beneficial and effective to consider the meaningful relations between the elements of learning style and science process skills in order to improve science process skills.

구조화된 간호중재 프로그램이 여중생의 월경곤란증 완화에 미치는 영향

최성희,김현옥 韓國學校保健學會 2001 韓國學校保健學會誌 Vol.14 No.1

The main purpose of this study is to identify the effects of a structured nursing intervention program on dysmenorrhea in middle school girls. This study employed two methods of research at the same time a quasi-experiment pre- and post-test to use for comparison. Subjects were middle school girls assigned to the research program: 40 girls in the second grade, 40 girls in the third grade at K Girls' Middle School and H Girls' Middle School located in J city. The data were collected for 68 days from August 23 to October 30, 1999. The dada were collected by observation technique and by a self-administered questionnaire. The instruments used for this study were as follows: the "Face Interval Pain Scale" menstrual pain instrument developed by McGreth(1987) and the "Menstrual Distress Questionnaire" dysmenorrhea instrument developed by Moos(1968). The dysmenorrhea knowledge instrument was developed by the researcher and refered to above. The research procedure was as follows: after preliminary examination, the experimental groups were provided educational programs with O.H.P. films developed by the researcher and with the videotape "first meeting" supported by P&G company and produced by EBS. The groups followed the educational programs twice for two weeks. At the beginning of menstruation, these subjects could choose one among the following choices: * Jacobson's progressively muscle relaxation method and massage treatment * Jacobson's progressively muscle relaxation method and hot bag treatment * Jacobson's progressively muscle relaxation method, a pain killer treatment, and massage treatment * Jacobson's progressively muscle relaxation method, a pain killer treatment, and hot bag treatment The dysmenorrhea of the control group were measured during the same period. The analysis of the collected data was done using an SPSS-PC+, descriptive statistic including real numbers, percentage, averages, standard deviations and t-test, χ2-test, and ANCOVA. The results of this study were as follows. (1) The knowledge level of the girls provided with the planned nursing intervention program was higher when compared to these who did not follow the educational programs. (2) The original difference in the dysmenorrhea level having been controled, the dysmenorrhea level made, nevertheless, a statistical difference between the experimental group and the control group(F=63.8, p= .00). Observed pain on quantity measurement of dysmenorrhea showed significant difference depending on the treatment(t=4.6, p= .00). In conclusion, those in the planned nursing intervention program had a higher knowledge level and fewer symptoms than the control group. Accordingly, the nursing intervention program for dysmenorrhea developed by the researcher can be effectively applied in nursing practice and can be the foundation for this kind of program.

단면형상변화에 따른 점용접 부재의 에너지흡수 특성

최영민,차천석,김지훈,서현경,양인영 한국공작기계학회 2006 한국공작기계학회 추계학술대회논문집 Vol.2006 No.-

The front-end side members of vehicles absorb the most of the energy during the front-end collision. These side members are mostly consist of single hat shape. The side members absorb more energy under axial load if they have higher strength, and stable folding capacity (local buckling). When structural members are subjected to axial loadings, stress is concentrated on their edges. Thus, in this study, the energy absorption characteristics of the member were analyzed according to change of sectional shape for increase of strength. The side members consist of single hat, double hat, single cap and double cap section member. Based on the static test result of these side member, the impact simulation were carried out and compared with test result.

20~21세기 한반도에서 국가적 성폭력과 그 희생제의로서 여성의 몸:일본군위안부, 여성빨치산, 그리고 여성탈북자의 삶을 중심으로

최현실 釜山大學校 韓國民族文化硏究所 2013 한국민족문화 Vol.46 No.-

Violence in the sacrifice of military sexual slavery by Japan, female partisans, and North Korean women defector’s body structure appeared in the 20~21 century, the Korean peninsula, there is a common feature of human rights exclusion. This study was to analyze the categorical content approach. The paper is organized as follows Deleuze’s agencement in the form of the major events of the life of the three study were placed into four steps. 1.Period of war and chaos in a woman’s body is reduced to sexual objectification. 2.Indelible traces of the body and trauma 3.Patriarchal society, anti-communist societies in a third country, life as a sinner, but a sinner 4.Gender go find a life worthy of human beings. Despite of the historical period and the beginning of the study subjects' discrimination, common symptoms of universal human rights and violence against a woman’s body was ruled out. Across the public and private areas of violence against a woman’s body, has created a space of exclusion of human rights. Despite of Individual distinctiveness, victims of violence put pressure on the woman’s body structure is characterized by repeated appear. For this shall continue to be a challenging task. 본 연구는 20~21세기 한반도에 나타났던 폭력에서 인권배제의 공통된 특징을 지닌, 일본군위안부와 여성빨치산, 여성탈북자의 몸의 희생 구조를 범주적 내용분석 중심으로 분석하였다.논문의 구성은 세 연구 대상의 생애의 주요한 사건을 들뢰즈의 아장스망(agencement)형식으로 네 단계로 배치하였다. 1.전쟁과 혼란의 시기에 여성의 몸이 성적 대상화로 전락, 2.지워지지 않는 몸의 흔적 그리고 트라우마, 3.가부장적 사회, 반공사회, 제3국에서의 죄인 아닌 죄인으로의 삶, 4. 젠더 인간다운 삶을 찾아가다 등이다. 연구 대상자들의 역사적 시기와 내용상의 차별에도 불구하고, 공통적으로 나타나는 현상은 여성의 몸에 대한 폭력과 보편적 인권의 배제이다. 여성의 몸에 대한 폭력은 공적 사적 영역을 넘나들며, 인권 배제의 공간을 창출하고 있다. 그리고 역사적 사건 속에서, 여성의 몸에 가하는 폭력의 희생 구조는 개별적 특수성에도 불구하고 반복되어 나타나는 특징이 있다. 이에 대한 도전 작업은 지속되어야 한다.

유리단 국소의치 장착 후 M.K.G.에 의한 하악운동 형태 및 속도의 변화

최현미,조주온,이상돈,송광엽,박찬운 全北大學校 齒醫學硏究所 1992 전북치대논문집 Vol.10 No.1

The purpose of this study is to evaluate the effects of distal extension partial denture on the mandibular movement patterns and velocity. The subjects are 10 patients who have unilateral or bilateral distal extension edentulous area in maxillary and/or mandibular arches. Their mandibular movement patterns and velocity had been measured by Mandibular Kinesiograph(M.K.G.-K6 diagnostic system, Myo-tronics Inc. Seattle. WA. U.S.A.) once just before the treatment and 8 times every week since the dentures were placed. The data have been analyzed statistically by means of ANOVA and Tukey test. The results have been obtained as follows : 1. The placement of the dentures has reduced the amount of vertical and antero-posterior displacement in the habitual mandibular movement significantly(p<0.01). 2. The maximum opening and closing velocity and the mean opening and closing velocity showed no significant change with the placement of the partial dentures(p>0.01). 3. The velocity trace of maximum opening-closing movement has been more regularized and well-coordinated, and the number of clicks decreased continuously after placement of the dentures and showed stable patterns since the 5th week(p<0.01). 4. The left deviation has been decreased significantly after the placement of the dentures and showed no significant change since the 4th week. The right deviation has also been reduced, but there was shown no difference after the 1st week(p<0.01). 5. The crossover pattern has been most frequently observed in the frontal plane and the sagittal plane. It has not been changed with the denture placement and stabilized uniformly.

설측브라켓 부착시 위치오차에 관한 연구

최준규,황현식 전남대학교 치과대학 1996 전남치대논문집 Vol.8 No.1

The purpose of this study was to evaluate the positioning errors according to the method of bonding lingual brackets. Dental models of twenty orthodontic patients with malocclusion were selected for this study. The positioning errors were measured on each model that brackets were bonded to . Three different bonding methods were used. For the first method, the bracket was bonded intimately to the lingual surface of the model. For the second method, the bracket was bonded intimately to the lingual surface after setting up using articulator. The passive bracketing, bonding the bracket ligated first to ideal archwire, was used after setting up as the last method. The results were as follows : 1. The brackets bonded without setting up showed greater angulation errors in the upper 1st premolar and the lower canine than those in other bonding methods. The brackets bonded without passive bracketing showed greater positioning errors in upper central incisor, lower 1st and 2nd premolars. 2. The brackets bonded without setting up showed greater torque error in lower 2nd premolar than those in other bonding methods. The brackets bonded without passive bracketing showed greater torque errors in all upper teeth, lower 1st and 2nd premolars. 3. The brackets bonded without passive bracketing showed greater rotation errors between upper central incisors, lower central incisors, lower lateral and central incisor, lower canine and lateral incisor. 4. The brackets bonded without setting up showed greater in-out errors between upper canine and lateral incisor than those in other bonding methods. The brackets bonded without passive bracketing showed greater in-out errors between upper central incisors, upper central and lateral incisors, upper 1st and 2nd premolars, lower lateral and central incisors, lower canine and lateral incisor. These results suggest that there is a large amount of positioning error in lingual brackets even by an indirect bonding technique, and it may be reduced by passive bracketing.