Determination of Acyl-lysophosphatidic Acid in Plasma by Tandem Mass Spectrometry

Yoon, Hye-Ran 덕성여자대학교 약학연구소 2005 藥學論文誌 Vol.16 No.1

Analysis of acyl lysophosphatidic acids(LPAs) has clinical importance as a potential biomarker for ovarian and other gynecological cancers or obesity in the aspect of prevention. Here we report simple sample preparation and analytical method developed with high sensitivity and specificity for the early detection of gynecological cancers to improve the overall outcome of this disease. We established a novel quantification method of acyl-LPAs in plasma by electrospray negative ionization tandem mass spectrometry (MS-MS) using multiple reaction monitoring mode without conventional TLC step. Protein-bound lipids, acyl-LPAs in plasma were extracted with methanol : chloroform (2:1) containing LPA C14:0 as internal standard under acidic condition. Following back extraction with chloroform and water, centrifuged lower phase was evaporated and reconstituted in methanol and then analyzed. Using ESI-MS-MS with negative ionization MRM mode, all the species of LPAs were completely separated from plasma matrix without severe interferences. For MRM mode, Q1 ions selected were m/z 409, 433, 435, 437 and 457 which corresponds to molecular mass [M-H]- of C16:0, C18:2, C18:1, C18:0 and C20:3 LPA, respectively. Q2 ions selected for MRM was m/z 79, phosphoryl product. Using MS/MS with MRM mode, all the species of LPAa were completely separated from plasma matrix without severe interferences. This method allowed simultaneous detection and quantification of different species of LPAs in a plasma over a linear dynamic range of 0.01 - 25 (mol/L. The method detection limit was 0.3 pmol/ml with correlation coefficient of 0.9983 in most LPAs analyzed. When applied to the plasmas with normal and gynecological cancer patient, this new method differentiated two different groups by way of total LPA level.

보리순의 영양성분 조성

서혜지,최용민,김상천,이준수,이기택,정명근,천지연,황진봉,남진식,서우덕,황영,김행란,김세나 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

유전성대사질환의 유전자치료

윤혜란,정성철 덕성여자대학교 약학연구소 2006 藥學論文誌 Vol.17 No.-

Inherited metabolic disease, inborn errors of metabolism is a group of genetic diseases defect in the metabolic pathways. Alterations in metabolic pathways are usually caused by defect of enzymes in the process. Generally, inherited metabolic diseases show heterogenous clinical features and symptoms may differ between siblings and families. Excess accumulation of precusors and their toxic derivatives in target tissues result in organ failures, such as central nervous system involvement and hepato-splenomegaly. Therapeutic starategies for inherited metabolic disease, including bone marrow transplantation, enzyme replacement therapy, and diet restriction, have been developed with some positive outcomes. However, these treatments could not be an ultimate therapeutic syarategy, their effects are temporary and give a burden to patients and their families economically and psychologically. Gene therapy can be a best choice for the treatment of inherited metabolic disease. Gene therapy involves the placement of missing genes into particular tissues by the harnessing of suitable vectors (originally these were animal viruses such as adenovirus). For example, gene therapy in the rat for diabetes has succeeded by liver-production of insulin (using genes obtained from pancreatic Islets of Langerhans cells). Many inborn errors of metabolism could be treated in this way: examples may include 100 haemoglobinopathies (such as sickle cell anaemia), phenylketonuria (PKU) etc. Here we present examples of gene therapy progress for Gaucher disease, Fabry disease, PKU, and MPS 1 (Hurlers syndrome) caused by lack of tissue-production of a particular enzyme (in its catalytically-active conformation).

초산모의 분만유형과 모성관련지식에 관한 연구

김혜란,노진아,손은정,이명아,김증임 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Purpose: This study was to know primipara's general characteristics, delivery type, delivery characteristics, maternity related knowledge level, and relation of delivery type and maternity related knowledge level. Method: This is a survey. Subjects were sixty primiparas who had admitted to a pediatric outpatient clinic at general hospital in Seoul. Subject's age was ranged from 15 to 44 and it was lapsed 10 weeks after delivery of their first baby. Maternity related knowledge level was measured by "primipara's maternity related knowledge level". Data was nalyzed using frequency. percentage, mean, standard deviation, ANOVA, t-test by SPSS 11.0. Result: Relation of delivery type and maternity related knowledge in primipary did not show a statistically significant. But the relation of natural delivery and maternity related knowledge in primipara was a littlehig than that of caesarean section. Conclusion: There is no significant diference of maternity related knowledge between natural delivery and caesarean section. We suggest for randomized clinical trials with a larger sample size.

자기감독 중재가 초등학교 학습장애 학생의 과제수행 행동 및 읽기 성취에 미치는 영향

정혜란 이화여자대학교 교육대학원 2003 이화교육논총 Vol.13 No.-

The purpose of this study was to investigate the effects of self-monitoring on improving leading on-task behavior and performance for elementary students with learning disabilities. For this purpose, three elementary students with learning disabilities were selected from a self-contained classroom of a general elementary school in Seoul. The multiple baseline design across subjects was used to examine the effects of intervention and maintenance. The students were trained how to moniter themselves utilizing self-recording and self-graphing strategies after doing the reading paragraphs. Maintenance was implemented 4 times in 3 weeks after fading the intervention. The results obtained in this study are summarized as follows: First, the reading on-task behavior was improved through self-monitoring techniques. Second, the rate of reading performance was improved through self-monitoring techniques. Third, the rate of correct response for reading on-task behavior was increased through self-monitoring techniques. Fourth, During the maintenance period, the reading on-task behavior and reading performance were maintained highly. Based on the results mentioned above, it could be concluded that overall, for elementary students with learning disabilities, self-monitoring had positive effects on increasing and maintaining the reading on-task behavior and reading performance. This result implies that self-monitoring technique through self-recording and self-photographing is an effective way for students with learning disabilities to perform their own learning invention program independently.

대학종합평가인정제 실시에 따른 대학도서관의 평가에 관한 연구

서혜란 한국문헌정보학회 1996 한국문헌정보학회지 Vol.30 No.1

현행 대학종합평가인정제의 실시배경과 목적, 평가절차, 평가영역 및 부문별 가중치를 개관하였다. 대학교육협의회에서 설정한 대학종합평가 기준 가운데 대학도서관에 관련된 평가항목 및 가중치, 평가지표를 분석하고 문제점을 지적했다. 외국에서의 대학 도서관 평가의 최근 경향을 살펴보고, 그것에 기초하여 현행 평가기준중 도서관 평가와 관련한 대안을 제시하였다. 단 평가 항목에 대한 정량적 평가척도 문제는 제외하였다 This study provides an overview of the accreditation landscape in Korea, and identifies some problems in the current accrediting review program which assesses the library's achievements. These are as follows: ①The weight given to the library sector is insufficient. ②There are no indicators to appraise the achievement of the informational and educational functions of academic libraries. ③It is completely devoted to input indicators, but not process or output indicators. ④It is impossible to evaluate the quality of library services. ⑤The units of statistics are not standardised. Some alternative measures and indicators are proposed to evaluate the performances of academic libraries* services. It is suggested that an official draft should be prepared by Korean academic librarians to amend the library sector of current accrediting review program

장애아동을 위한 원예치료 : Effect of HT as a Altemative Therapy, Especially in the United States 대체치료의 일환으로서의 원예치료, 미국의 사례를 중심으로

곽혜란,Relf, Paula Diane 韓國植物ㆍ人間ㆍ環境學會 2000 인간식물환경학회지 Vol.3 No.1

The disability has meaning of either physical or mental impairment restricting motality, communication, self-care, and interpersonal skills, etc., and requires manifold levels of rehabilitation system. Especially in the case of disabled children, the disability may result in causing serious problems of reduction in much potentialities they deserve in the early stage of development. Children's disability includes trauma of cerebral palsy, spinabifida, neurogical, spiral cord, and brain impairments, etc. The problem of theses disabilities arise in muscle motors such as fine-and gross-motor skills, the coordination as visual motor cooperation, or in cognition of maintaining the concentration ability, or of long and short memorizings. Various alternative therapies must be needed for the children in order to gain their rehabilitation and social restoration influences, and are recently common in the United States. Horticultural therapy being one of them is known to have noted benefits for the disabled with multi-motor skills, responsibility for taking care of raising plants, cognitive skills of self-esteem, and motivation. Horticultural therapy either alone in combination with other alternative means as music or pet therapy for the disabled children have also been documented often, and known to increase subscaling in depression, hyperactivity, social interaction, depression, anxiety and intellectual screening. Horticultural activities by the disabled children are likewise supposed to provide them with a wise range of educational opportunities through various practical hand-on experiences. These information of the beneficial activity are the example of Rusk Institute of Rehabilitation Medicine and Kluge Children's Rehabilitation Center recently operating in the United State.

여대생의 체위, 식습관 및 영양실태 조사

신혜란,박영미 효성여자대학교 가정대학 학도호국단 1987 家政大論集 Vol.6 No.-

본 연구에서는 효성여대 3, 4학년 학생 96명을 대상으로 여대생들의 바람직한 건강관리와 영양지도를 위해서 이들의 체위와 식습관 영양소 섭취상태를 조사하여 다음과 같은 결과를 얻었다. (1) 본 조사 대상자들의 체위는 신장 160∼162㎝가 전체의 24%로 가장 많았고 154∼156㎝가 20%, 158∼160㎝가 17%의 분포를 보였고 평균치는 158.2㎝로 나타났다. 체중은 47.5∼50㎏이 24%, 50∼52.5㎏이 17%, 45∼47.5, 52.5∼55㎏이 15%의 분포를 보였고 평균치는 49.7㎏으로 나타났다. Skinfold Thickness는 16∼18㎜24%, 10∼12㎜, 12∼14㎜가 15%, 18∼20㎜는 11%의 분포를 나타났고 이들의 평균치는 17.1㎜였다. (2) 식습관 평균점수는 10점 만점에서 4.44였고 식습관을 다시 5등급으로 분류 했을 때 다소 불량으로 판단되는 2등급에 속하였다. (3) 영양소 섭취상태는 열량 1431.1㎉, 단백질 55.1gm, 철 14.7㎎으로서 권장량에 비해 부족상태이고 칼슘, 아스코르빈산, 티아민, 리보플라빈, 나이아신은 각각 559.5㎎, 52.7㎎, 1.1㎎, 1.2㎎, 16.6㎎으로서 권장량과 거의 일치하므로서 모두 양호한 편이다. 총 열량 구성에 있어서 당질 단백질 지방이 차지하는 비율을 보면 단백질은 55gm로 15.4%를 차지하고 지방은 30.8gm로 19.5%를 차지하고 당질은 총 열량중 65%를 차지하는 것으로 나타났다. For the purpose of implementing health and nutrition education for college women, we investigatednutritional status of college women related to health status, food habit and nutrient intake. The survey was conducted for ten days, from November 20 to 30, 1986. The subjects of this survey were 96 students of Hyosung women's University. The results of health status, food habit and nutrient intake of college womens were as follows; 1. Average height, weight and skinfoldthinckness were height(158.2cm) weight(49.7kg) skinfold-thickness(17.1mm). 2. Average nutrient intake of college women junior sinior per day. Calorie intake was 1431kcal. This was composed of carbohydrate(65%), protein 55.1gm(15.4%), and fat 30.8gm(19.5%) other nutrients such as calcium(559.5gm) iron(147mg), vitamin A(5104.9I.U.) and Thiamin(1.1mg) Riboflavin(1.2mg) Niacin(16.6mg) ascorbicacid(52.7mg).

한국 공공도서관 재정의 역사적 고찰

서혜란 新羅大學校 1992 論文集 Vol.34 No.1

The purpose of this study is to investigate and evaluate the financial policies of public libraries in Korea historically. The movement of public library establishment by the patriotic intellectuals with their own private endowment in the end of 19th century was all in vain with the Japanese annexation of Korea. National and local finances in straits, lack of understanding of the public library and defects of the Library Act of 1963 made the public libraries in the Republic of Korea suffer from the limited budgets. Library Act of 1963 was amended in 1987. The revised act obliged municipalities to have a public library for the first time. It was not until the amendment that the general account of the local finance was stipulated as revenue sources for the public libraries. But charging for the admission of public library was sanctioned under the pretext of difficulties of public finance. Library Promotion Act which substituted Library Act of 1987 prohibited the admission charges as of January 1, 1992. Government grants did not make great contribution to the development of public library services. And we can be so optimistic about the success of the grants planning. Public libraries in Korea, despite of some improvements, have failed to secure the stable revenue sources. They constantly find themselves suffering the effects of cutbacks in budgets. So a new and positive strategic planning is in need for more prosperous future.

광중합 시 수종의 심미적 수복재와 이장재의 사용에 따른 치수내 온도변화

장혜란,이형일,이광원,이세준 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.5

Polymerization of light-activated restorations results in temperature increase caused by both the exothermic reaction process and the energy absorbed during irradiation. Within composite resin, temperature increases up to 20℃ or more during polymerization. But, insulation of hard tissue of tooth lowers this temperature increase in pulp. However, many clinicians are concerned about intrapulpal temperature injury. The purpose of this study was to evaluate temperature changes in the pulp according to various restorative materials and bases during curing procedure. Caries and restoration-free mandibular molars extracted within three months were prepared Class I cavity of 3×6mm with high speed handpiece fissure bur. 1mm depth of dentin was evaluated with micrometer in mesial and distal pulp horns. Pulp chambers were filled with 37.0 ±0.1℃ water to CEJ. Chromium-alumina thermocouple was placed in pulp horn below restorative materials for evaluating of temperature changes. This thermocouple was connected to temperature-recording device(Multifunction analyzer MX, 6.000, JAPAN). Temperature changes was evaluated from initial 37.0℃ after temperature changes to 37.0℃. Tip of curing unit was placed in the center of prepared cavity separated 1mm from restorative materials. Curing time was 40s. The restorative materials were used with Z 100, Fuji II LC, Compoglass flow and bases were used with Vitrebond. Dycal. Resrorative materials were placed in 2mm. The depth of bases were formed in 1mm and in this upper portion, resin of 2mm depth was placed. This procedure was performed 10 times. The results were as follows : 1.All the groups showed that the temperature in pulp increased as curing time inceased 2.The temperature increase of glass ionomer was significantly higher than that of Resin and Compomer during curing procedure (P<0.05). 3.The temperature increase In glass ionomer base was significantly higher than that of Calcium hydroxide base during Resin curing procedure (P<0.05).