Polycaprolactone Triol–Citrate Scaffolds Enriched with Human Platelet Releasates Promote Chondrogenic Phenotype and Cartilage Extracellular Matrix Formation

Hussin A. Rothan,Suhaeb A. Mahmod,Ivan Djordjevic,Mojtaba Golpich,Rohana Yusof,Simmrat Snigh 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.2

In this paper we report the differentiating properties of platelet-rich plasma releasates (PRPr) on human chondrocytes within elastomeric polycaprolactone triol–citrate (PCLT–CA) porous scaffold. Human-derived chondrocyte cellular content of glycosaminoglycans (GAGs) and total collagen were determined after seeding into PCLT–CA scaffold enriched with PRPr cells. Immunostaining and real time PCR was applied to evaluate the expression levels of chondrogenic and extracellular gene markers. Seeding of chondrocytes into PCLT–CA scaffold enriched with PRPr showed significant increase in total collagen and GAGs production compared with chondrocytes grown within control scaffold without PRPr cells. The mRNA levels of collagen II and SOX9 increased significantly while the upregulation in Cartilage Oligomeric Matrix Protein (COMP) expression was statistically insignificant. We also report the reduction of the expression levels of collagen I and III in chondrocytes as a consequence of proximity to PRPr cells within the scaffold. Interestingly, the pre-loading of PRPr caused an increase of expression levels of following extracellular matrix (ECM) proteins: fibronectin, laminin and integrin b over the period of 3 days. Overall, our results introduce the PCLT–CA elastomeric scaffold as a new system for cartilage tissue engineering. The method of PRPr cells loading prior to chondrocyte culture could be considered as a potential environment for cartilage tissue engineering as the differentiation and ECM formation is enhanced significantly.

Strobilanthes crispus Juice Concentrations and Anticancer Effects on DNA Damage, Apoptosis and Gene Expression in Hepatocellular Carcinoma Cells

Hussin, Faridah,Eshkoor, Sima Ataollahi,Rahmat, Asmah,Othman, Fauziah,Akim, Abdah,Eshak, Zolkapli Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.14

Background: Hepatocellular carcinoma is one of the most common cancers worldwide. Its prevalence is increasing in many countries. Plant products can be used to protect against cancer due to natural anticancer and chemopreventive constituents. Strobilanthes crispus is one of plants with potential chemopreventive ability. Objective: This study aimed to evaluate the anticancer effects of Strobilanthes crispus juice on hepatocellular carcinoma cells. Materials and Methods: MTT assays, flow cytometry, comet assays and the reverse transcription-polymerase chain reaction (RT-PCR) were used to determine the effects of juice on DNA damage and cancer cell numbers. Results: This juice induced apoptosis after exposure of the HepG2 cell line for 72 h. High percentages of apoptotic cell death and DNA damage were seen at the juice concentrations above 0.1%. It was found that the juice was not toxic for normal cells. In addition, juice exposure increased the expression level of c-myc gene and reduced the expression level of c-fos and c-erbB2 genes in HepG2 cells. The cytotoxic effects of juice on abnormal cells were in dose dependent. Conclusions: It was concluded that the Strobilanthes crispus juice may have chemopreventive effects on hepatocellular carcinoma cells.

The Quest for Leadership Legitimacy among Singapore Malays

Hussin Mutalib 서울대학교행정대학원 2012 Asian Journal of Political Science Vol.20 No.1

Much has been written about the problems and concerns affecting the Malay ethnicminority in Singapore. These include issues such as their socio-economic situation,relatively low educational performance, and increasing incidences of social ailments likehigh rates of drug addiction, divorce and youth delinquency. In the context of Singapore’smultiracial and multi-religious fabric, little has been articulated about what is hereargued to be a core issue of the ‘Malay plight’, namely, the legitimacy of the Malaypolitical leadership. Apparently both the ruling-party-affiliated Malay politicians andtheir Malay opposition counterpart have been at odds with their perceptions andpredispositions towards this particular issue. It is here that a bold, albeit controversial,alternative model called the ‘collective leadership’, was publicly goaded by the Associationof Malay/Muslim Professionals in both their 1990 and 2000 National Conventions,which requires due consideration. Perhaps at a time of a changing Singapore society, thisalternative or its variant could go some way towards mitigating, if not resolving, thelong-standing Malay plight, and thus, benefit not only the Malay minority itself, butSingapore as a whole.

An Enhanced Task Scheduling Algorithm on Cloud Computing Environment

Hussin M. Alkhashai,Fatma A. Omara 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.7

Cloud computing is the technology that moves the information technology (IT) services out of the office. Unfortunately, Cloud computing has faced some challenges. The task scheduling problem is considered one of the main challenges because a good mapping between the available resources and the users' tasks is needed to reduce the execution time of the users’ tasks (i.e., reduce make-span), and increase resource utilization. The objective of this paper is to introduce and implement an enhanced task scheduling algorithm to assign the users' tasks to multiple computing resources. The aim of the proposed algorithm is to reduce the execution time, and cost, as well as, increase resource utilization. The proposed algorithm is considered an amalgamation of the Particle Swarm Optimization (PSO),the Best–Fit (BF), and Tabu-Search (TS) algorithms; called BFPSOTS. According to the proposed BFPSOTS algorithm, the BF algorithm has been used to generate the initial population of the standard PSO algorithm instead of to be random. The Tabu-Search (TS) algorithm has been used to improve the local research by avoiding the trap of the local optimality which could be occurred using the standard PSO algorithm. The proposed hybrid algorithm (i.e., BFPSOTS) has been implemented using Cloudsim. A comparative study has been done to evaluate the performance of the proposed algorithm relative to the standard PSO algorithm using five problems with different number of independent task, and Virtual Machines (VMs). The performance parameters which have been considered are the execution time (Makspan), cost, and resources utilization. The implementation results prove that the proposed hybrid algorithm (i.e., BFPSOTS) outperforms the standard PSO algorithm..

Characterization of the first mitogenomes of the smallest fish in the world, Paedocypris progenetica, from peat swamp of Peninsular Malaysia, Selangor, and Perak

Hussin, NorJasmin,Azmir, Izzati Adilah,Esa, Yuzine,Ahmad, Amirrudin,Salleh, Faezah Mohd,Jahari, Puteri Nur Syahzanani,Munian, Kaviarasu,Gan, Han Ming Korea Genome Organization 2022 Genomics & informatics Vol.20 No.1

The two complete mitochondrial genomes (mitogenomes) of Paedocypris progenetica, the smallest fish in the world which belonged to the Cyprinidae family, were sequenced and assembled. The circular DNA molecules of mitogenomes P1-P. progenetica and S3-P. progenetica were 16,827 and 16,616 bp in length, respectively, and encoded 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and one control region. The gene arrangements of P. progenetica were identical to those of other Paedocypris species. BLAST and phylogenetic analyses revealed variations in the mitogenome sequences of two Paedocypris species from Perak and Selangor. The circular DNA molecule of P. progenetica yield a standard vertebrate gene arrangement and an overall nucleotide composition of A 33.0%, T 27.2%, C 23.5%, and G 15.5%. The overall AT content of this species was consistent with that of other species in other genera. The negative GC-skew and positive AT-skew of the control region in P. progenetica indicated rich genetic variability and AT nucleotide bias, respectively. The results of this study provide genomic variation information and enhance the understanding of the mitogenome of P. progenetica. They could later deliver highly valuable new insight into data for phylogenetic analysis and population genetics.

The Effects of Glutamate NMDA Receptor Antagonist MK-801 on Gastrointestinal Motility after Middle Cerebral Artery Occlusion in Rats

Nasir Hussin Ameer,이재희,최명애,Guang-Shi Jin,김미선,박병림 대한약리학회 2010 The Korean Journal of Physiology & Pharmacology Vol.14 No.3

This study was performed to investigate the role of glutamate neurotransmitter system on gastrointestinal motility in a middle cerebral artery occlusion (MCAO) model of rats. The right middle cerebral artery was occluded by surgical operation, and intestinal transit and geometric center as a parameter of gastrointestinal motility and expression of c-Fos protein in the insular cortex and cingulate cortex were measured at 2 and 12 h after MCAO. Intestinal transit was 66.3±7.5% and 62.3±5.7% 2 and 12 h after sham operation, respectively, and MCAO significantly decreased intestinal transit to 39.0±3.5% and 47.0±5.1% at 2 and 12 h after the occlusion, respectively (p<0.01). The geometric center was 5.6±0.4 and 5.2±0.9 at 2 and 12 h after sham operation, respectively, and MCAO significantly decreased geometric center to 2.9±0.8 and 3.0±0.3 at 2 and 12 h after the occlusion, respectively (p<0.01). In control animals, injection of atropine decreased intestinal transit to 35.9±5.2%, and injection of glutamate NMDA receptor antagonist, MK-801, decreased intestinal transit to 28.8±9.5%. Pretreatment with MK-801, a glutamate NMDA receptor antagonist, in the MCAO group decreased intestinal transit to 11.8±3.2%, which was significantly decreased compared to MCAO group (p<0.01). MCAO markedly increased the expression of c-Fos protein in the insular cortex and cingulate cortex ipsilateral to the occlusion 2 h after MCAO, and pretreatment with MK-801 produced marked reduction of c-Fos protein expression compared to MCAO group (p<0.01). These results suggest that modulation of gastrointestinal motility after MCAO might be partially mediated through a glutamate NMDA receptor system.

Structural and Electrical Properties of Bi0.5Na0.5TiO3 Templates Produced by Topochemical Microcrystal Conversion Method

Ali HUSSIN,Adnan Maqbool,Rizwan Ahmed Malik,김민수,송태권,김명호,Arif Zaman,김원정 한국물리학회 2015 새물리 Vol.65 No.8

Bi0.5Na0.5TiO3 (BNT) templates with a single phase perovskite structure were produced from a plate-like precursor particles of bismuth-layer-structured ferroelectric Bi4.5Na0.5Ti4O15 (BNT4) through a topochemical microcrystal conversion process. First, the plate-like BNT4 precursor particles were prepared via molten salt synthesis. The layered structure BNT4 transformed into a single phase perovskite BNT templates after its topochemical reaction with the complementary Na2CO3 and TiO2 reactants at 950 ℃ for 4 h in a NaCl flux. The as synthesized BNT templates exhibited large grains (range from 10 to 15 μm), had plate-like morphology and exhibits a single-phase perovskite structure with a pseudo-cubic symmetry. Furthermore, the temperature dependences of dielectric constant and loss at different frequencies showed a relaxor behavior, and polarization versus electric field curves exhibited a typical ferroelectric response.

Malaysian Food Heritage, Hospitality, and Dietary: between Culture and Education

Hanafi Hussin 한국식생활문화학회 2017 한국식생활문화학회학술대회논문집 Vol.2017 No.11

Review: Reorienting Orientalism

Iza Hussin 서울대학교행정대학원 2008 Asian Journal of Political Science Vol.16 No.2

Goals, components, and factors considered in university development

Sufean Hussin 서울대학교 교육연구소 2009 Asia Pacific Education Review Vol.10 No.1

It is generally known that the general goals of universities are to produce high-quality graduates for the job market, to continuously advance the frontier of knowledge in all the disciplines, and ultimately to advance human civilization. There can, however, be numerous specific goals which differ from one university to the other. Whatever it is, in order to achieve the desired goals, universities need to design a realistic strategic development plan, which must include, among others, the important logistical components and factors of university development. In Malaysia, little is known about university development planning process as a subject area. Annual reports and calendars published by universities do not provide sufficient information about the elements and aspects of high priorities in their strategic development plan. As such, we decided to conduct a study on eleven (11) foremost public universities in Malaysia-a country with a centralized education system-in order to examine the goals, components, and factors considered by university top-management in planning university development. We administered a checklist to 296 respondents, comprising deputy vice-chancellors or deputy rectors, registrars, deans, and directors. The checklist required the respondents to rate the consideration level on a scale of 1 (least considered) to 5 (highly considered) for each of the items on goals, components, and factors. This article reports the main findings of our study. Among other things, the most obvious result revealed by the study was that universities were very concerned with the relevancy of academic programs offered and their performance in research. It was rather surprising, however, that the top-management of public universities in Malaysia placed the goal of providing quality infrastructure and facilities at the lowest ranking. Also, this study revealed that the government, as a factor, exerted its prominence only in terms of university budget and research grants and the execution of some policies of national interest, but university expansion and development was largely driven by the university organization itself, i.e., on where, what, and how it wants to expand and grow. In this regard, public universities in Malaysia still enjoy a large degree of academic autonomy and a strong support by the government.