Mitochondrial citrate accumulation drives alveolar epithelial cell necroptosis in lipopolysaccharide-induced acute lung injury

Yang Hui-Hui,Jiang Hui-Ling,Tao Jia-Hao,Zhang Chen-Yu,Xiong Jian-Bing,Yang Jin-Tong,Liu Yu-Biao,Zhong Wen-Jing,Guan Xin-Xin,Duan Jia-Xi,Zhang Yan-Feng,Liu Shao-Kun,Jiang Jian-Xin,Zhou Yong,Guan Cha-Xi 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Necroptosis is the major cause of death in alveolar epithelial cells (AECs) during acute lung injury (ALI). Here, we report a previously unrecognized mechanism for necroptosis. We found an accumulation of mitochondrial citrate (citratemt) in lipopolysaccharide (LPS)-treated AECs because of the downregulation of Idh3α and citrate carrier (CIC, also known as Slc25a1). shRNA- or inhibitor–mediated inhibition of Idh3α and Slc25a1 induced citratemt accumulation and necroptosis in vitro. Mice with AEC-specific Idh3α and Slc25a1 deficiency exhibited exacerbated lung injury and AEC necroptosis. Interestingly, the overexpression of Idh3α and Slc25a1 decreased citratemt levels and rescued AECs from necroptosis. Mechanistically, citratemt accumulation induced mitochondrial fission and excessive mitophagy in AECs. Furthermore, citratemt directly interacted with FUN14 domain-containing protein 1 (FUNDC1) and promoted the interaction of FUNDC1 with dynamin-related protein 1 (DRP1), leading to excessive mitophagy-mediated necroptosis and thereby initiating and promoting ALI. Importantly, necroptosis induced by citratemt accumulation was inhibited in FUNDC1-knockout AECs. We show that citratemt accumulation is a novel target for protection against ALI involving necroptosis.

Expression and function analysis of a rice OsHSP40 gene under salt stress

Xin Wang,Huan Zhang,Lu‑Yuan Shao,Xin Yan,Hui Peng,Jie‑Xiu Ouyang,Shao‑Bo Li 한국유전학회 2019 Genes & Genomics Vol.41 No.2

Heat shock proteins (HSPs) play essential roles in both plant growth and abiotic stress tolerance. In rice, OsHSP40 was recently reported to regulate programmed cell death (PCD) of suspension cells under high temperature. However, the expression and functions of OsHSP40 under normal growth or other abiotic stress conditions is still unknown. We reported the expression and function of a rice OsHSP40 gene under salt stress. Homologous proteins of OsHSP40 were collected from the NCBI database and constructed the neighbor-joining (NJ) phylogenetic tree. The expression pattern of OsHSP40 was detected by qRT-PCR under NaCl (150 mM) treatment. Then, identified a rice T-DNA insertion mutant oshsp40. At last, we compared and analyzed the phenotypes of oshsp40 and wild type under salt stress. OsHSP40 was a constitutively expressed small HSP (sHSP) gene and was close related to other plant sHSPs. Moreover, the expression of OsHSP40 was regulated by salt, varying across time points and tissues. Furthermore, the growth of T-DNA insertion mutant of OsHSP40 (designated as oshsp40) was suppressed by NaCl (150 mM) compared with that of the WT at seedling stage. Detailed measurement showed root and shoot length of the oshsp40 seedlings were significantly shorter than those of the WT seedlings under NaCl stress. In addition, the pot experiment results revealed that seedlings of oshsp40 withered more seriously compared with those of WT after NaCl treatment and recovery, and that survival rate and fresh weight of oshsp40 seedlings were significantly reduced. Taken together, these data suggested that OsHSP40 had multiple functions in rice normal growth and abiotic stress tolerance.

A Review on Microbial Decaffeination

Hui-Shi Wu,Xin-Qiang Zheng,Yue-Rong Liang,Jian-Hui Ye,Jian-Liang Lu 한국차학회 2015 한국차학회지 Vol.- No.S

Exclusive exposure of caffeine might bring some adverse effects to human health, and cause some risks to environment. Microbial decaffeination might be a new alternative approach because of its high specificity and efficiency. Many studies revealed that several microorganisms can degrade caffeine, including genera Alcaligenes,Rhodococcus, Klebsiella, Acinetobacter, Enterobacter, Stenotrophomonas and Pseudomonas. Biodegradation of caffeine can be significantly influenced by temperature, pH, aeration rate and initial caffeine concentration of the incubation. There are two main pathways of caffeine biodegradation: C-8 oxidation and N-demethylation, while N-demethylation is the general catabolism pathway in bacteria. Application of microbial decaffeination has also been discussed in this review.

A practical headspace gas chromatographic method for the determination of oxalate in bleaching effluents

Hui-Chao Hu,Hui-Jun Jin,Xin-Sheng Chai 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.1

This paper reports an improved headspace gas chromatographic (HS-GC) technique for thedetermination of oxalate content in bleaching effluents. The method is based on the redox reactionbetween potassium iodate and oxalate, from which the carbon dioxide is generated and measured by HSGC. The results showed that the reaction in a sulfuric acid solution (0.05 mol/L) was complete in 35 minat 95 ℃. The RSD of the method in the repeatability testing was less than 4.5%, the LOQ was 0.35 mg, andthe recovery ranged from 95 to 103%. The present method is simpler, more reliable, and more practicalcompared to the methods reported previously.

Differential expression profile of microRNA in yak skeletal muscle and adipose tissue during development

Hui Ji,Hui Wang,Qiumei Ji,Winbo Ji,Xiaolin Luo,Jiabo Wang,Zhixin Chai,Jinwei Xin,Xin Cai,Zhijuan Wu,Jikun Wang,Jincheng Zhong 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background miRNAs play an important role in regulating normal animal development. Muscle tissue and fat metabolismare important for maintaining energy balance in animals. Yak has important agricultural and economic importance as itprovides milk, meat, and hair. It is used for transportation as well. However, the miRNA expression profiles of their muscleand adipose tissue are currently unknown. Objective To explore the regulatory roles of miRNAs in the skeletal muscle and adipose tissues of yak. Methods A total of 12 small RNA libraries were constructed from the skeletal muscle and adipose samples from yak aged 0.5,2.5, 4.5, and 7.5 years. High-throughput sequencing and bioinformatics analysis were used to determine the dynamic expressionprofile of miRNA, and a miRNA regulatory network related to muscle and adipose tissue development was established. Results miR-1-3p and miR-143-3p showed the highest expression during yak skeletal muscle and fat development, respectively. The MAPK and Ras signaling pathways were the pivotal pathways. miR-181-5p, miR-542-3p, and miR-424-5p mayhave key roles in skeletal muscle development, and CREBRF, GRB10, CDK1, RFX3, and EPC2 were the core target genes. While miR-127-5p, miR-379-3p, and miR-494-3p may play important regulatory roles in adipose deposition, and ETV1,XPO7, and C5AR2 were the core target genes. Conclusion This study provides valuable resources for further study of the molecular mechanisms underlying yak skeletalmuscle and adipose tissue development, and also a basis for studying the interactions between genes and miRNAs.

Part three: Community Health Education and Development : TB Training in FuGong County of NuJiang

( Hui Xin Wu ) 한국환경보건학회(구-한국환경위생학회) 2004 Current Challenges and Advances in Environmental H Vol.- No.-

Protective effects of phillyrin against influenza A virus in vivo

Xin-yan Qu,Qing-jun Li,Hui-min Zhang,Xiao-juan Zhang,Peng-hui Shi,Xiu-juan Zhang,Jing Yang,Zhe Zhou,Sheng-qi Wang 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.7

Influenza A virus infection represents a great threat to public health. However, owing to side effects and the emergence of resistant virus strains, the use of currently available anti-influenza drugs may be limited. In order to identify novel anti-influenza drugs, we investigated the antiviral effects of phillyrin against influenza A virus infection in vivo. The mean survival time, lung index, viral titers, influenza hemagglutinin (HA) protein and serum cytokines levels, and histopathological changes in lung tissue were examined. Administration of phillyrin at a dose of 20 mg/kg/day for 3 days significantly prolonged the mean survival time, reduced the lung index, decreased the virus titers and interleukin-6 levels, reduced the expression of HA, and attenuated lung tissue damage in mice infected with influenza A virus. Taken together, these data showed that phillyrin had potential protective effects against infection caused by influenza A virus.

3D hierarchical transition-metal sulfides deposited on MXene as binder-free electrode for high-performance supercapacitors

Hui Li,Xin Chen,Erfan Zalnezhad,K.N. Hui,K.S. Hui,Min Jae Ko 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.82 No.-

MXene has been considered as a promising two-dimensional material for supercapacitors owing to itslarge surface area, high conductivity, and excellent cycling stability. However, its low specific capacitancerestricts its extensive applications. Therefore, to address the issue, we homogeneously deposited NiCo2S4nanoflakes on the surface of MXene on conductive nickel foam (denoted as MXene-NiCo2S4@NF), whichwas used as a composite binder-free electrode for supercapacitor applications. The NiCo2S4 nanoflakesincreased the surface area of the composite electrode, thereby increasing its specific capacity from106.34 C g 1 to 596.69 C g 1 at 1 A g 1. Compared to the pristine MXene, MXene-NiCo2S4@NF maintainedthe high retention rate of pristine MXene and exhibited excellent cycling stability with 80.4% of its initialspecific capacity after 3000 cycles. The composite electrode exhibited improved electrochemicalperformance for supercapacitors, owing to the combined merits of NiCo2S4 (high specific capacity) andMXene (high retention rate and good cycling stability. The fabricated asymmetric solid-statesupercapacitor using MXene-NiCo2S4 as a positive electrode and active carbon as a negative electrode,exhibited an energy density of 27.24 Wh kg 1 at 0.48 kW kg 1 of power density.

The Effect of Mn/Al Substitution on the Structural Stability and Magnetic Properties of Mn₃AlC

Xin-You Wang,Ping-Zhan Si,Hui-Dong Qian,Yang Yang,Hong-Liang Ge,Jihoon Park,Xin-Qing Wang,Chul-Jin Choi 한국자기학회 2019 Journal of Magnetics Vol.24 No.1

The structural stability and magnetic properties of Mn3+xAl1-xC antiperovskite with varied Mn/Al substitution were studied systematically. Single phase Mn3+xAl1-xC alloys with antiperovskite structure were obtained in samples with x = −1/4, 0, 1/4, 1/2. An additional Mn23C₆ phase was precipitated from Mn3+xAl1-xC antiperovskite for x = 3/4 while Mn23C₆ phase was formed as major phase for x = 1. The mutual substitution of Mn and Al atoms has substantial effect on the Curie temperature and the saturation magnetization of the Mn3+xAl1-xC alloys. In comparison with the as-cast alloys, the as-annealed Mn3+xAl1-xC alloys exhibit reduced Mn/Al substitutions after high temperature homogenization, which enhances the ordering of Mn and Al atoms in the lattices. The Curie temperature of the homogenized Mn3+xAl1-xC increases with increasing Mn substitution to Al. The Mn₃AlC alloy shows the highest saturation magnetization among all samples with varied Mn/Al ratios. Most samples show zero coercivity and zero remanent magnetization. The maximum value of the magnetic entropy changes of Mn2.75Al1.25C at 285 K is 2.26 J/㎏ K in fields up to 3 T.

녹색신용대출이 중국상업은행의 신용위험에 미치는 영향

Hui-Xin Zhao,제해금 한국유통경영학회 2022 유통경영학회지 Vol.25 No.1

Purpose: The purpose of this study was to examine how green credit loans affect the credit risk of commercial banks in China. The heterogeneity of banks was analyzed, and the synergy with the equity capital ratio was analyzed more innovative in consideration of the impact of the government’s regulatory policy on green credit loans. Research design, data, and methodology: This study focuses on the influence of green credit on the risk of commercial banks in China. Using the sample data of 16 relatively large commercial banks in China from 2009 to 2019, this paper constructs a static panel data model for empirical analysis. Results: First, The results show that the green credit business can significantly reduce the non-performing loan ratio (NPLR) of banks. Second, We found that the green credit ratio (GCR) is positively correlated with the NPLR namely, the green credit ratio and credit risk show a "U-shaped" relationship between credit risk and "pre-down, post-up". Third, The impact of green credit on the credit risk of non-state-owned banks is significantly reduced, while the impact on the credit risk of state-owned commercial banks is not significant, Finally, In view of the interaction of capital adequacy ratio(CAR) and green credit on bank credit risk, we find that banks with higher capital adequacy ratio have a more positive effect on reducing credit risk. Implications: Commercial banks that develop the green credit have risk consideration, especially credit risk. It should be noted that risk management considerations are important for the stability of the banking system. This study provides empirical evidence for commercial banks to reduce the credit risk. The results of this study are helpful in actively promoting China’s green credit loan policy.