IGF-1 from Adipose-Derived Mesenchymal Stem Cells Promotes Radioresistance of Breast Cancer Cells

Yang, Hui-Ying,Qu, Rong-Mei,Lin, Xiao-Shan,Liu, Tong-Xin,Sun, Quan-Quan,Yang, Chun,Li, Xiao-Hong,Lu, Wei,Hu, Xiao-Fang,Dai, Jing-Xing,Yuan, Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Purpose: The aim of this study was to investigate effects of adipose-derived mesenchymal stem cells (AMSCs) on radioresistance of breast cancer cells. Materials and Methods: MTT assays were used to detect any influence of AMSC supernatants on proliferation of breast cancer cells; cell migration assays were used to determine the effect of breast cancer cells on the recruitment of AMSCs; the cell survival fraction post-irradiation was assessed by clonogenic survival assay; ${\gamma}$-H2AX foci number post-irradiation was determined via fluorescence microscopy; and expression of IGF-1R was detected by Western blotting. Results: AMSC supernatants promoted proliferation and radioresistance of breast cancer cells. Breast cancer cells could recruit AMSCs, especially after irradiation. IGF-1 derived from AMSCs might be responsible for the radioresistance of breast cancer cells. Conclusions: Our results suggest that AMSCs in the tumor microenvironment may affect the outcome of radiotherapy for breast cancer in vitro.

One simple, rapid and economical method for ploidy detection of Trichogramma dendrolimi Matsumura (Hymenoptera Trichogrammatidae)

Liu Quan-quan,Zhang Chen,Zhou Jin-cheng,Dong Qian-jin,Huo Liang-xiao,Dong Hui 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2

Ploidy diversity provides valuable scientific information, thus making the detection technique of ploidy important. However, traditional methods of cytological observation and flow cytometry are either laborious or expensive. We here report a simple and rapid, effective and economical quantitative PCR (qPCR) approach to determine the ploidy of a parasitoid species Trichogramma dendrolimi Matsumura, an economically important biocontrol agent. We applied a mitochondrial gene cytochrome oxidase (COI) and a nuclear gene forkhead to evaluate the mitochondrial number per nuclear copy in a thelytokous Wolbachia-infected strain of T. dendrolimi and its bisexual uninfected counterparts. The 2 −ΔCq values calculated from C q values which resulted from qPCR experiments were significantly larger in haploid males than that in diploid females. Haploid males possessed about 2.69 times mitochondrial number per nuclear copy as diploid females. Not a single significant difference was found between diploid females from thelytokous and bisexual strains. Based on the differences in relative mitochondrial content, we were allowed to distinguish between haploid males and diploid females. Moreover, the number of mitochondria significantly decreased with higher ploidy level but was not affected by Wolbachiainfection. Our study supplied an available tool to investigate the ploidy diversity in sex determination of T. dendrolimi and thelytokous manipulation of Wolbachia, which is the crucial step to further study their underlying mechanisms. This will in turn contribute to the biocontrol efficiency by enhancing the female production and hence the parasitism rate.

The Stent-Assisted Coil-Jailing Technique Facilitates Efficient Embolization of Tiny Cerebral Aneurysms

Cong-Hui Li,Xian-Hui Su,Bo Zhang,Yong-Feng Han,Er-Wei Zhang,Lei Yang,Dong-Liang Zhang,Song-Tao Yang,Zhen-Quan Yan,Bu-Lang Gao 대한영상의학회 2014 Korean Journal of Radiology Vol.15 No.6

Objective: Tiny cerebral aneurysms are difficult to embolize because the aneurysm’s sac is too small for a single small coil, and coils within the aneurysm may escape from the confinement of a stent. This study was performed to introduce the stent-assisted coil-jailing technique and to investigate its effect on the coil embolization of tiny intracranial aneurysms. Materials and Methods: Sixteen patients with tiny intracranial aneurysms treated with the stent-assisted coil-jailing technique between January 2011 and December 2013 were retrospectively reviewed and followed-up. Results: All aneurysms were successfully treated with the coil-jailing technique, and at the end of embolization, complete occlusion of the aneurysm was achieved in 9 cases (56.3%), incomplete occlusion in 6 (37.5%), and partial occlusion in 1 (6.3%). Intraprocedural complications included acute thrombosis in one case (6.3%) and re-rupture in another (6.3%). Both complications were managed appropriately with no sequela. Follow-up was performed in all patients for 3–24 months (mean, 7.7 months) after embolization. Complete occlusion was sustained in the 9 aneurysms with initial complete occlusion, progressive thrombosis to complete occlusion occurred in the 6 aneurysms with initial near-complete occlusion, and one aneurysm resulted in progressive thrombosis to complete occlusion after initial partial occlusion. No migration of stents or coils occurred at follow-up as compared with their positions immediately after embolization. At follow-up, all patients had recovered with no sequela. Conclusion: The stent-assisted coil-jailing technique can be an efficient approach for tiny intracranial aneurysms, even though no definite conclusion regarding its safety can be drawn from the current data.

Note on genus Achlya Billberg, 1820 from China (Lepidoptera, Thyatiridae)

Zhao-Hui PAN,Yong-Quan ZHOU,Hui-Lin HAN 한국곤충학회 2010 Entomological Research Vol.40 No.4

The genus Achlya Billberg, 1820 is reported for the first time from China as A. jezoensis (Matsumura, 1927), which is a species collected from Maorshan, Heilongjiang Province. The adult and genitalia characters of the species are briefly redescribed and illustrated, distribution data is provided.

Adaptive compensation method for real-time hybrid simulation of train-bridge coupling system

Hui M. Zhou,Bo Zhang,Xiao Y. Shao,Ying P. Tian,Wei Guo,Quan Gu,Tao Wang 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.83 No.1

Real-time hybrid simulation (RTHS) was applied to investigate the train-bridge interaction of a high-speed railway system, where the railway bridge was selected as the numerical substructure, and the train was physically tested. The interaction between the two substructures was reproduced by a servo-hydraulic shaking table. To accurately reproduce the high-frequency interaction responses ranging from 10-25Hz using the hydraulic shaking table with an inherent delay of 6-50ms, an adaptive time series (ATS) compensation algorithm combined with the linear quadratic Gaussian (LQG) was proposed and implemented in the RTHS. Testing cases considering different train speeds, track irregularities, bridge girder cross-sections, and track settlements featuring a wide range of frequency contents were conducted. The performance of the proposed ATS+LQG delay compensation method was compared to the ATS method and RTHS without any compensation in terms of residual time delays and root mean square errors between commands and responses. The effectiveness of the ATS+LQG method to compensate time delay in RTHS with high-frequency responses was demonstrated and the proposed ATS+LQG method outperformed the ATS method in yielding more accurate responses with less residual time delays.

A New Algorithm to Improve Efficiency of Resource Scheduling in Clouding Computing Based on Extended Support Vector Machine

Quan Gan,Jun-Hui Zheng 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.3

ONE-STEP SYNTHESIS OF BIOCOMPATIBLE CHITOSAN/NaGdF 4 :Eu 3 þ NANOCOMPOSITE WITH FLUORESCENT AND MAGNETIC PROPERTIES FOR BIOIMAGING

HUI LI,QUAN LIN,CHUANXI WANG,YINGNAN JIANG,ZHANCHEN CUI 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.1

Lanthanide-doped luminescent nanoscale materials have great potential applications in biologicalresearches. Herein, we reported a novel and mild method for one-step synthesis of chitosan/NaGdF 4 :Eu 3 þnanocomposites. The luminescent Eu 3 þions and magnetic resonance imaging(MRI) contrast agent Gd 3 þions were incorporated to these biocompatible nanocomposites. Theresultant nanocomposites exhibited strong °uorescence and attractive magnetic features. Thenanocomposites also have pure hexagonal phase with uniform size of about 65 nm. FT-IR spectrarevealed that these nanocomposites were successfully coated by hydrophilic chitosan, whoseamine groups conferred the nanocomposites excellent dispensability in aqueous solution. Besides,the MTT assay and laser confocal microscopy images have con¯rmed the good biocompatibilityof the nanocomposites. These results indicated that the as-prepared nanocomposites could beused as an excellent targeted imaging agent in biological felds.

외국인 유학생의 전자우편 분석 연구

전미화(Quan, Mei-hua),김월휘(Jin, Yue-hui) 우리말글학회 2014 우리말 글 Vol.61 No.-

본 연구는 한국어 전자우편 쓰기의 중요성을 강조하고 외국인 유학생들의 전자우편에 나타난 특성을 살피는 것을 목적으로 한다. 이를 위해 외국인 유학생들이 학교와 일상생활에서 관계 맺는 다양한 부류의 사람을 대상으로 쓴 전자우편 112편을 구조와 표현적 측면에서 분석하였다. 구조적 측면에서 분석한 결과 외국인 유학생들의 전자우편에는 제목을 쓰지 않거나 서론과 결미 부분에서 인사말과 보내는 이의 서명을 생략하는 경우가 있었고, 사연 부분에서는 화용적 기능에 따라 나타난 구조가 학습자 별로 너무 다양하여 전자우편 쓰기라는 장르에 대한 지식이 보편화되지 못하였음을 알 수 있었다. 또한 표현적 측면에서 높임법의 사용은 선어말어미의 활용이 압도적으로 많은 반면 오류는 조사의 사용에서 가장 많이 나타났고, 다양한 결속기제가 사용되고 있었으나 구와 문장 간의 결속력을 확보하는 데 실패하는 경우가 많았으며, 첫인사와 끝인사에서 관용적인 표현을 활용하고자 하였으나 그러한 표현이 정확한 인사로 이어지지 못하고 있음을 알 수 있었다. 본 연구는 실제적인 전자우편 쓰기에서의 특성 및 문제점을 파악하여 한국어 쓰기 교육에 유용한 교육 자료로 활용할 수 있다는 점에서 의의를 가진다. The purpose of this research is to emphasize the significance of email writing in the Korean language. It has been carried out by observing the features shown in the emails of overseas students. To attain this aim, a thorough analysis has been conducted on the structure and expression level of 112 emails written by overseas students in their efforts to establishing relationship with various people in their daily school lives. The results of the analysis on the structural level show that certain items are found missing in the emails written by overseas students, such as a lack of titles or introduction in the beginning of the email, as well as no greeting words and signature at the end. Various structures are presented in accordance with the function of the text in the body part. Moreover, skills on email writing have not been popularized yet and complement suffix is very flexible in the use of honorifics at the expression level. However, most mistakes take place in the use of auxiliary words. Though lots of conjunctions are used, there is also an abundance in incorrectly joining phrases and sentences, such as the common expressions of first-time greetings and end greetings. Those ways of expressions, however, cannot form fluent and accurate greetings. This research probes into the features and crucial points of real-time email writing. It constitutes useful teaching materials and has great importance in teaching Korean writing in the future.

Evaluation of the anti-Toxoplasma gondii Activity of Hederagenin in vitro and in vivo

Run-Hui Zhang,Runhao Jin,Hao Deng,Qing-Kun Shen,Zhe-Shan Quan,Chun-Mei Jin 대한기생충학열대의학회 2021 The Korean Journal of Parasitology Vol.59 No.3

Toxoplasma gondii infection is widespread worldwide, not only posing a serious threat to human food safety and animal husbandry, but also endangering human health. The selectivity index was employed to measure anti-T. gondii activity. Hederagenin (HE) exhibited potent anti-T. gondii activity and low cytotoxicity. For this reason, HE was selected for in vivo experiments. HE showed 64.8%±13.1% inhibition for peritoneal tachyzoites in mice, higher than spiramycin 56.8%±6.0%. Biochemical parameters such as alanine aminotransferase, aspartate aminotransferase, glutathione, and malondialdehyde, illustrated that HE was a good inhibitor of T. gondii in vivo. This compound was also effective in relieving T. gondii-induced liver damage. Collectively, it was demonstrated that HE had potential as an anti-T. gondii agent.

Genetic Diversity and Structure of Cordyceps sinensis Populations from Extensive Geographical Regions in China as Revealed by Inter-Simple Sequence Repeat Markers

Hong-Hui Liang,Zhou Cheng,Xiao-Ling Yang,Shan Li,Zu-Quan Ding,Tong-Shui Zhou,Wen-Ju Zhang,Jia-Kuan Chen 한국미생물학회 2008 The journal of microbiology Vol.46 No.5

Cordyceps sinensis is one of the most valuable medicinal caterpillar fungi native to China. However, its productivity is extremely limited and the species is becoming endangered. The genetic diversity of eighteen C. sinensis populations across its major distributing regions in China was evaluated by inter-simple sequence repeat (ISSR) markers. A total of 141 markers were produced in 180 individuals from the 18 populations, of which 99.3% were polymorphic. The low average of Shannon (0.104) and Nei index (0.07) of the 18 populations indicates that there are little genetic variations within populations. For all 18 populations, estimates of total gene diversity (HT), gene diversity within populations (HS), coefficient of genetic differentiation (GST), and gene flow (Nm) were 0.170, 0.071, 0.583, and 0.357, respectively. This pattern suggests that the genetic diversity of C. sinensis is low and most of the ISSR variations are found among populations with little gene exchange. The 18 populations are divided into five groups based on the genetic distance and the grouping pattern matches with the geographic distribution along the latitudinal gradient. The five groups show obvious difference in the GST and Nm values. Therefore, the genetic diversification of C. sinensis populations may be determined by geographic isolation and the combined effects of life history characters and the interaction with host insect species. The information illustrated by this study is useful for selecting in situ conservation sites of C. sinensis.