Novel fabrication of microRNA nanoparticles-coated coronary stent for prevention of post-angioplasty restenosis

Hui-Lian Che,In-Ho Bae,Kyung-Seob Lim,Saji Uthaman,In Taek Song,이해신,이두환,김원종,Young Keun Ahn,박인규,Myung Ho Jeong 대한심장학회 2016 Korean Circulation Journal Vol.46 No.1

Background and Objectives: MicroRNA 145 is known to be responsible for cellular proliferation, and its enhanced expression reportedly inhibits the retardation of vascular smooth muscle cell growth specifically. In this study, we developed a microRNA 145 nanoparticle immobilized, hyaluronic acid (HA)-coated stent. Materials and Methods: For the gene therapy, we used disulfide cross-linked low molecular polyethylenimine as the carrier. The microRNA 145 was labeled with YOYO-1 and the fluorescent microscopy images were obtained. The release of microRNA 145 from the stent was measured with an ultra violet spectrophotometer. The downstream targeting of the c-Myc protein and green fluorescent protein was determined by Western blotting. Finally, we deployed microRNA 145/ssPEI nanoparticles immobilized on HA-coated stents in the balloon- injured external iliac artery in a rabbit restenosis model. Results: Cellular viability of the nanoparticle-immobilized surface tested using A10 vascular smooth muscle cells showed that MSN exhibited negligible cytotoxicity. In addition, microRNA 145 and downstream signaling proteins were identified by western blots with smooth muscle cell (SMC) lysates from the transfected A10 cell, as the molecular mechanism for decreased SMC proliferation that results in the inhibition of in-stent restenosis. MicroRNA 145 released from the stent suppressed the growth of the smooth muscle at the peri- stent implantation area, resulting in the prevention of restenosis at the post-implantation. We investigated the qualitative analyses of in- stent restenosis in the rabbit model using micro-computed tomography imaging and histological staining. Conclusion: MicroRNA 145-eluting stent mitigated in-stent restenosis efficiently with no side effects and can be considered a successful substitute to the current drug-eluting stent.

A Physiological Disorder of Saline Stress Released Greenhouse Tomato Plants

Hui-lian Xu,Andre Gosselin 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Biological Pest and Disease Control in Greenhouse Vegetable Production

Hui-lian Xu 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Anti-cancer therapy using nanofibers for combined delivery of drug and gene in liver cancer

Hui-Lian CHE,Kondareddy CHERUKULA,Yeonsoo JUNG,In-Kyu PARK 한국생물공학회 2015 한국생물공학회 학술대회 Vol.2015 No.4

Effect of Ambient Humidity on Photosynthesis of Greenhouse Tomato Plants

Hui-Lian Xu,Andre Gosselin 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Analysis of The Coupling Behavior of a Thin Layer of a Temperature-pH Dual Sensitive Hydrogel for an Inhomogeneous Large Deformation

Hui-Xian Yan,Hai-Lian Hong,Lian-Hui Zheng,Heng-Di Su 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.73 No.7

Due to ionic migration and adsorption, an electrical double layer with a thickness scaled by the Debye length is formed at the hydrogel-solution interface. For a thin temperature-pH dual sensitive hydrogel whose chickness is comparable to the Debye length, its mechanical behavior may be affected by the electrical double layer. In this paper, by incorporating electromagnetic field theory into thermodynamic theory, we develop a coupled field theory for a temperature-pH dual sensitive hydrogel, and we take into account the electrical double layer. The proposed model can be used to analyze the in uences of the size effect, ionic concentration, ambient temperature and solution pH value on the swelling of a thin layer of the temperature-pH sensitive hydrogel.

한국 사회통합정책에 대한 이주민의 인식과 수요조사 연구: 재한 중국인의 사례를 중심으로

김혜련 ( Hui Lian Jin ) 성결대학교 다문화평화연구소 2014 다문화와 평화 Vol.8 No.2

이주의 시대가 도래됨에 따라 한국의 이주민도 급증하고 있다. 2014년 현재 한국에 체류 하고 있는 외국인은 이미 160만 명을 초과하였다. 이는 한국사회의 다문화·다인종화가 가속화되고 있음을 의미한다. 앞으로 한국 체류 이주민은 계속 증가하고, 그에 따라 인종, 민족, 문화 다양성도 증대될 것이며, 이러한 다양성을 어떻게 통합할 것인가가 중요한 국가적 과제로 부상하고 있다. 이러한 배경 하에 이 연구는 재한 중국인을 대상으로 설문조사를 실시하여 정책 대상자 입장에서 사회통합정책을 분석하였다. 즉, 현행 사회통합정책 에 대한 이주민의 정책 만족도와 수요를 조사하여 정책의 문제점을 파악하였다. 연구 결 과, 이주민은 한국사회 적응과정에서 차별과 편견으로 어려움을 겪고 있으며, 사회통합정 책에 대한 만족도가 높지 않다는 반응을 보였다. 또한 이주민의 수요를 간과한 채, 획일적으로 사회통합정책을 추진한다고 응답하고 있다. 이러한 연구 결과를 기반으로, 이 연구는 맞춤형 사회통합정책 추진, 이주민 정치참여 확대, 사회통합 시민교육 추진, 사회통합 전문 가 양성 등 새로운 정책 방향을 제시하였다. As the era of migration has come, immigrants is rapidly increasing in Korea. In 2014, the number of the foreigners who are staying in Korea is already more than 1.6 million. It indicates that Korea is rapidly becoming a multicultural and multiethnic society. As foreign residents in Korea continue to increase, racial, ethnic and cultural diversity will increase in the future. So, how the diversity integrate will emerge as an important national task. In this context, the study analyzes social integration policy from the view of object of policy through conducting a survey targeting Chinese in Korea. In other words, it tries to find out what the problems are by investigating immigrant``s policy satisfaction and demand on social integration policy. According to the results of the research, immigrants are experiencing difficulties by discrimination and prejudice in the process of adaptation to Korean society and the level of satisfaction on social integration policy appeared to be low. Moreover, the current social integration policy has been promoted uniformly, starting at the national interests but overlooking the immigrant``s demand. Based on these findings, the study shows the new policy directions such as promoting customized social integration policy, expanding political participation of immigrant, guarantee of social equality of immigrant through the provision of laws and system, driving civic education and training professionals for social integration, etc.

Selection of reference genes for RT-qPCR analysis in Trichogramma chilonis (Hymenoptera: Trichogrammatidae)

Xie Lian-Cheng,Tian Jun-Ce,Lu Yan-Hui,Xu Hongxing,Zang Lian-Sheng,Lu Zhongxian,Jin Lin-Hong 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Trichogramma chilonis is an important natural enemy for control of various Lepidoperan crop pests. The biology of T. chilonis is well-studied, but the molecular mechanisms of this biology require further study. Screening suitable reference genes is a vital step for use of RT-qPCR to understand underlying molecular physiology. In the present study, nine candidate reference genes including elongation factor 2 (EF2), ribosomal proteins (RPS23, RPL13, and RPL44), malate dehydrogenase (MDH), eukaryotic translation initiation factor 3 subunit F (EIF3F), zinc finger protein 268 (ZFP268), muscle specific protein 20 (MP20), and ATP synthase subunit alpha (ATP5F1A) were evaluated at different conditions including development stage, diet, temperature, and insecticide treat ments. Four common algorithms (the Delta Ct method, geNorm, BestKeeper, and NormFinder) and RefFinder were used to analyze gene expression stability. Our results indicated that two reference genes used for normalization were sufficient, and the optimal combinations were: RPS23 and EF2 for developmental stages, ZFP268 and EF2 for feeding with different diets, ZFP268 and RPL13 for temperature treatments, and EF2 and RPL44 for insec ticide treatments. The results provide preliminary determination of suitable reference gene for standard RT-qPCR analyses in T. chilonis, which might establish the foundation for further molecular biology research.

말레이시아 화인디아스포라의 모국관계 연구

김혜련 ( Hui Lian Jin ) 한국민족연구원 2015 민족연구 Vol.0 No.61

다민족·다문화국가 말레이시아에는 약 640만 명의 화인이 거주하고 있으며, 이는 말레이시아 전체 인구의 24.6%를 차지한다. 말레이시아 화인은 대부분 중국 푸젠(福建), 광둥(□東), 광시(□西), 하이난(海南)지역 출신이며, 모국에 대한 ‘집합적 기억’을 중심으로 그들만의 화인공동체를 형성하고 있다. 이 연구는 디아스포라와 모국의 연관성이라는 문제의식에서 출발하여 쿠알라룸푸르에서 진행한 설문조사를 중심으로 말레이시아 화인디아스포라와 모국과의 관계를 분석하였다. 연구결과, 말레이시아 화인은 비록 현지적응을 통해 주류사회에 진출하였으나, 여전히 화인으로서의 민족정체성을 유지하고 있으며, 모국어를 능통하게 구사하고 있을 뿐만 아니라, 모국문화도 계승하고 발전시키고 있다. 이러한 모국지향성을 기반으로 화인은 비록 모국과의 정치적 연계는 미약하나, 경제 및 사회·문화적 영역에서 모국인 중국과 밀접한 연계를 유지하고 있다. 더불어 모국과의 이러한 경제 및 사회·문화적 관계는 향후에도 계속 발전할 것으로 전망된다. About 6.4 million of Chinese live in Malaysia where is multiethnic and multicultural country and they occupy 24.6percent of the Malaysian population. The majority of Chinese in Malaysia came from Fujian, Guangdong, Guangxi, and Hainan in China and perform Chinese Diaspora community with collective memory. This research analyzes the relations between Chinese diaspora in Malaysia and motherland, started from critical mind on links between diaspora and motherland, through conducting a survey. According to the result of the research, even though Chinese diaspora in Malaysia entered into mainstream society through local adaptation, they still not only maintain ethnic identity but also inherit and develop their own language and culture. Based on the motherland directivity, Chinese diaspora have not a strong political connection with motherland but maintain a close connection with China in economic and sociocultural aspects.

Biodegradable particulate delivery of vascular endothelial growth factor plasmid from polycaprolactone/polyethylenimine electrospun nanofibers for the treatment of myocardial infarction.

Che, Hui-Lian,Muthiah, Muthunarayanan,Ahn, Youngkeun,Son, Sejin,Kim, Won Jong,Seonwoo, Hoon,Chung, Jong Hoon,Cho, Chong-Su,Park, In-Kyu American Scientific Publishers 2011 Journal of nanoscience and nanotechnology Vol.11 No.8

<P>In this study, we present nanofiber-mediated gene delivery for myocardial infarction (MI). Branched polyethylenimine cross-linked via disulfide bonds (ssPEI) complexed with vascular endothelial growth factor (VEGF) were immobilized on electrospun polycaprolactone (PCL)/polyethylenimine (PEI) nanofibers for the local expression of VEGF angiogenic factor. We studied whether the production of VEGF from myoblast cells adhering on the nanofibers has therapeutic potential for MI. In this method, the non-specific adsorption of VEGF nanoparticles to the nanofibers occurred uniformly over all of the surface area of the nanofibers, resulting in increased transgene uptake and expression in a great number of cells. The amount of DNA required for transfection was also minimal compared to bolus delivery, because the adhered DNA was directly available in the cell microenvironment, which also helps in localized delivery. Reporter genes luciferase (Luc), red fluorescence protein (RFP), and therapeutic gene VEGF were tested to evaluate the transfection efficiency of ssPEI nanoparticles immobilized on the nanofiber surface. Our results demonstrated that the delivery of therapeutic genes from biodegradable nanoparticles immobilized on the nanofiber represented minimal cytotoxicity of H9C2 myoblasts than branched PEI 25 kDa did. According to Luc assay, fluorescence microscope analysis, and reverse transcription polymerase chain reaction (RT-PCR), this vector showed high transgene expression efficiency to the reporter gene and VEGF gene. The surface-mediated delivery of the DNA nanoparticles did not adversely affect cell growth, and facilitated the transgene expression inside the cells.</P>