Production of a Recombinant Laccase from Pichia pastoris and Biodegradation of Chlorpyrifos in a Laccase/Vanillin System

( Hui fang Xie ),( Qi Li ),( Min Min Wang ),( Lin Guo Zhao ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.6

The recombinant strain P. pastoris GS115-lccC was used to produce laccase with high activity. Factors influencing laccase expression, such as pH, methanol concentration, copper concentration, peptone concentration, shaker rotate speed, and medium volume were investigated. Under the optimal conditions, laccase activity reached 12,344 U/L on day 15. The recombinant enzyme was purified by precipitating and dialyzing to electrophoretic homogeneity, and was estimated to have a molecular mass of about 58 kDa. When guaiacol was the substrate, the laccase showed the highest activity at pH 5.0 and was stable when the pH was 4.5~6.0. The optimal temperature for the laccase to oxidize guaiacol was 60℃, but it was not stable at high temperature. The enzyme could remain stable at 30℃ for 5 days. The recombinant laccase was used to degrade chlorpyrifos in several laccase/mediator systems. Among three synthetic mediators (ABTS, HBT, VA) and three natural mediators (vanillin, 2,6-DMP, and guaiacol), vanillin showed the most enhancement on degradation of chlorpyrifos. Both laccase and vanillin were responsible for the degradation of chlorpyrifos. A higher dosage of vanillin may promote a higher level of degradation of chlorpyrifos, and the 2-step addition of vanillin led to 98% chlorpyrifos degradation. The degradation of chlorpyrifos was faster in the L/V system (kobs = 0.151) than that in the buffer solution (kobs = 0.028).

Association of Polymorphisms in Stress-Related TNFα and NPY Genes with the Metabolic Syndrome in Han and Hui Ethnic Groups

Bu, De-Yun,Ji, Wen-Wu,Bai, Dan,Zhou, Jian,Li, Hai-Xia,Yang, Hui-Fang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: Metabolic syndrome (MS) is a cluster of complicated disorders caused by the interactive influencing factors of heredity and environment, which predisposes to many cnacers. Results from epidemic research indicate that stress is tightly related to the pathogenesis of MS and neoplasia. This paper aims to investigate the association between psychological stress and MS with respect to the tumor necrosis factor alpha (TNF${\alpha}$) and neuropeptide Y (NPY) genes in the Han and Hui ethnic groups. Methods: All subjects for this case-control study matched strict enrollment criteria (nationality, gender and age) and lived in the city of Wu Zhong of Ningxia Province in China. The enrolled group contained 102 matched pairs of Hui ethnic individuals and 98 matched pairs of Han ethnic individuals. Enrolled subjects completed the general Symptom Checklist-90 (SCL-90). The TNF${\alpha}$-308G/A variant and NPYrs16147 polymorphism were detected in case (81 males, 119 females) and control (81 males, 119 females) groups by polymerase chain reaction (PCR) amplification. Results: Nine factors of the SCL-90 were found to be statistically different (p<0.05) between case and control groups. The homozygous mutant genotype (AA) and the mutant allele (A) of the TNF${\alpha}$-308G/A gene were less frequently observed in the control population compared to the case group. The odds ratio (95% confidence interval) in "Allele" for MS was 2.28 (1.47-3.53), p=0.0001, while "OR" was 1.11 (0.83-1.47), p=0.15, for the NPYrs16147 gene polymorphism. Conclusions: Psychological stress has been positively associated with MS. A previous study from our group suggested there were differences in the level of psychological stress between Hui and Han ethnic groups. Furthermore, we found that the stress-related TNF${\alpha}$ gene was associated with MS for both Han and Hui ethnic groups. In contrast, NPY may be a possible contributor to MS and associated cancer for the Han ethnic group.

Serum Uric Acid Relation for Hearing Threshold Shiftt

Hui-Fang Yang,Tung-Wei Kao,Tao-Chun Peng,Yu-Shan Sun,Fang-Yih Liaw,Chung-Ching Wang,Ju-Ting Hsueh,Wei-Liang Chen 대한이비인후과학회 2017 Clinical and Experimental Otorhinolaryngology Vol.10 No.2

Objectives. The effects of serum uric acid (UA) level on a variety of diseases were found from experimental and observational studies via oxidative stress and anti-oxidants. However, research on the association of UA and hearing thresholds is relatively sparse. We investigated this issue in the U.S. general population to evaluate the relationship of serum UA levels and pure tone threshold of hearing. Methods. Forty four thousand eighty four eligible participants aged 20 to 69 years who have serum UA data and received Audiometry Examination Component were enrolled from the National Health and Nutrition Examination Survey 1999–2004. Hearing thresholds (dB) as a pure tone average at low frequencies (0.5, 1, 2 kHz) and at high frequencies (3, 4, 6, and 8 kHz) were computed. Multivariate linear regression models and tertile-based analysis with an extended-model approach for covariates adjustment were used to assess the correlation between serum UA level and hearing thresholds. Results. In the adjusted mode of tertile-based analysis, the regression coefficients elucidated as the change of log-transformed mean hearing thresholds upon comparing participants in the highest tertile of serum UA to those in the lowest tertile were –0.067 (P=0.023) in high frequency and –0.058 (P=0.054) in low frequency. After adjusting for multiple pertinent covariates, inverse association between tertiles of serum UA and hearing thresholds remained essentially unchanged. The negative trends between serum UA and hearing thresholds were statistically significant (P for trends <0.05) in tertile-based multiple linear regressions. Conclusion. Individuals with elevated UA levels independently were found to be inversely associated with hearing thresholds for pure tone audiometry in a nationally representative sample of U.S. adults.

High-Performance Single Layered WSe₂ p-FETs with Chemically Doped Contacts

Fang, Hui,Chuang, Steven,Chang, Ting Chia,Takei, Kuniharu,Takahashi, Toshitake,Javey, Ali American Chemical Society 2012 Nano letters Vol.12 No.7

<P>We report high performance p-type field-effect transistors based on single layered (thickness, ∼0.7 nm) WSe<SUB>2</SUB> as the active channel with chemically doped source/drain contacts and high-κ gate dielectrics. The top-gated monolayer transistors exhibit a high effective hole mobility of ∼250 cm<SUP>2</SUP>/(V s), perfect subthreshold swing of ∼60 mV/dec, and <I>I</I><SUB>ON</SUB>/<I>I</I><SUB>OFF</SUB> of >10<SUP>6</SUP> at room temperature. Special attention is given to lowering the contact resistance for hole injection by using high work function Pd contacts along with degenerate surface doping of the contacts by patterned NO<SUB>2</SUB> chemisorption on WSe<SUB>2</SUB>. The results here present a promising material system and device architecture for p-type monolayer transistors with excellent characteristics.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2012/nalefd.2012.12.issue-7/nl301702r/production/images/medium/nl-2012-01702r_0001.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl301702r'>ACS Electronic Supporting Info</A></P>

Comparison of Leukotriene Receptor Antagonist and Theophylline in Addition to Inhaled Corticosteroid in Adult Asthma: A Meta-Analysis

( Hui Juan Fang ),( Jian Miao Wang ),( Di Jin ),( Yong Cao ),( Yong Jian Xu ),( Wei Ning Xiong ) 한국응용약물학회 2011 Biomolecules & Therapeutics(구 응용약물학회지) Vol.19 No.3

This meta-analysis was performed to evaluate the difference of the therapeutic efficacy and adverse effects of leukotriene receptor antagonist and theophylline added to inhaled corticosteroids in adult asthma. Databases were searched for studies published through Nov, 2010. Randomized-controlled trials containing inhaled corticosteroids plus leukotriene receptor antagonist and inhaled corticosteroids plus sustained-release theophylline for asthma therapy were selected. For each report, data were extracted to the outcomes analyzed: mean change in morning peak expiratory flow, mean change in evening peak expiratory flow, mean change in morning forced expiratory volume in 1 sec, mean change in daily short bete2-agonist use, asthma exacerbation and adverse effects. Four assessable trials including 182 asthmatic patients were identified. Inhaled corticosteroids plus leukotriene receptor antagonist was superior to inhaled corticosteroids plus theophylline therapy in improving morning peak expiratory flow in asthmatics (mean difference 19.08 [95% confidence interval 13.37-23.79] l/min, p<0.001) and morning forced expiratory volume in 1 sec in asthmatics (mean difference 0.09 [95% confidence interval 0.03-0.14] liter, p=0.001). In evening peak expiratory flow, daily short bete2-agonist use, asthma exacerbation and adverse effects, there was no significant difference between these two therapies (All p>0.05). Our meta-analysis showed that the combination of inhaled corticosteroids plus leukotriene receptor antagonist resulted in more improvement in both peak expiratory flow and forced expiratory volume in 1 sec in the morning than inhaled corticosteroids plus sustained-release theophylline in adult asthmatics. Further trials are necessary to evaluate the dominant effects of the former combination.

Relationship of Work Stress, Professional Commitment and Job Satisfaction among Nurse Practitioners in Taiwan

Hui-Fang Yeh,Chien-Lin Kuo,Su-Fen Cheng,Kuei-Yun Lu 한국간호과학회 2009 한국간호과학회 학술대회 Vol.2009 No.10

Slimy partners: the mucus barrier and gut microbiome in ulcerative colitis

Fang Jian,Wang Hui,Zhou Yuping,Zhang Hui,Zhou Huiting,Zhang Xiaohong 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Ulcerative colitis (UC) is a chronic recurrent intestinal inflammatory disease characterized by high incidence and young onset age. Recently, there have been some interesting findings in the pathogenesis of UC. The mucus barrier, which is composed of a mucin complex rich in O-glycosylation, not only provides nutrients and habitat for intestinal microbes but also orchestrates the taming of germs. In turn, the gut microbiota modulates the production and secretion of mucins and stratification of the mucus layers. Active bidirectional communication between the microbiota and its ‘slimy’ partner, the mucus barrier, seems to be a continually performed concerto, maintaining homeostasis of the gut ecological microenvironment. Any abnormalities may induce a disorder in the gut community, thereby causing inflammatory damage. Our review mainly focuses on the complicated communication between the mucus barrier and gut microbiome to explore a promising new avenue for UC therapy.

miR-638 is a new biomarker for outcome prediction of non-small cell lung cancer patients receiving chemotherapy

Fang Wang,Jian-fang Lou,Yan Cao,Xin-hui Shi,Peng Wang,Jian Xu,Er-fu Xie,Ting Xu,Rui-hong Sun,Jianyu Rao,Pu-wen Huang,Shi-yang Pan,Hong Wang 생화학분자생물학회 2015 Experimental and molecular medicine Vol.47 No.-

MicroRNAs (miRNAs), a class of small non-coding RNAs, mediate gene expression by either cleaving target mRNAs or inhibiting their translation. They have key roles in the tumorigenesis of several cancers, including non-small cell lung cancer (NSCLC). The aim of this study was to investigate the clinical significance of miR-638 in the evaluation of NSCLC patient prognosis in response to chemotherapy. First, we detected miR-638 expression levels in vitro in the culture supernatants of the NSCLC cell line SPC-A1 treated with cisplatin, as well as the apoptosis rates of SPC-A1. Second, serum miR-638 expression levels were detected in vivo by using nude mice xenograft models bearing SPC-A1 with and without cisplatin treatment. In the clinic, the serum miR-638 levels of 200 cases of NSCLC patients before and after chemotherapy were determined by quantitative real-time PCR, and the associations of clinicopathological features with miR-638 expression patterns after chemotherapy were analyzed. Our data helped in demonstrating that cisplatin induced apoptosis of the SPC-A1 cells in a dose- and time-dependent manner accompanied by increased miR-638 expression levels in the culture supernatants. In vivo data further revealed that cisplatin induced miR-638 upregulation in the serum derived from mice xenograft models, and in NSCLC patient sera, miR-638 expression patterns after chemotherapy significantly correlated with lymph node metastasis. Moreover, survival analyses revealed that patients who had increased miR-638 levels after chemotherapy showed significantly longer survival time than those who had decreased miR-638 levels. Our findings suggest that serum miR-638 levels are associated with the survival of NSCLC patients and may be considered a potential independent predictor for NSCLC prognosis.

중국 북방 지구 商나라 말기~西周전기 "夷式簋"의 전파와 족군 변동

Fang Hui(方輝) 한국상고사학회 2014 한국상고사학보 Vol.85 No.-

"夷式簋"는 상나라 말기~서주 전기 商式簋ㆍ周式簋와 형식적 계보를 달리하면서 黃河 유역에 공존하고 있던특징적인 토기이다. 夷式簋는魯겗지구에서가장먼저 출현하였는데, 그계보는魯겗지구의岳石文化로까지거슬러 올라간다. 魯겗지구의夷式簋는주로商나라말기의고분에서 출토되는데, 商式簋와같은고분군에공존하고 있으면서도 단일 고분에는 결코 동반되지 않는다. 이러한 점을 고려할 때, 夷式簋는 商나라 때 夷人족군을 상징하는 표지물이었던 것으로 여겨진다. 夷式簋는 魯北 지구를 빼면 商나라 말기의 도성이었던 安陽 殷墟에서도 확인되는데, 이러한 발견 정황은 商나라와 夷族이 서로 상호 작용을 하였던 것과 관련이 있다. 安陽 殷墟 일대의 夷式簋는 고분 외에 폐기구덩이와 문화층에서도 출토되는데, 이러한 출토 정황은 원래 夷式簋가 제작되던 지역을 벗어난 곳에서도 계속적으로 제작되었다는 점과 함께 사용상의 일정한 변화 또한 발생하였음을 시사한다. 夷式簋는商나라말기~西周전ㆍ중기陝西省의周原과甘肅省의 河西走廊일대까지 확산되었는데, 이는 商나라 말기 秦人의 서쪽으로의 이주와 周나라가 商나라를 멸망시킨 이후 일부 東夷族이 周나라지배집단에딸려 서쪽으로 이주된 것과 연관이 있다. So-called Yi-style Gui-pot was a kind of pottery that distributed in the Yellow River valley in the period of late Shang to early Zhou dynasty, which existed with other two different style Gui-pots, Shang-style and Zhou-style Gui-pots at the same time. Yi-style Gui pot origin from northern Shandong province, and its earliest type could be traced back to the Yueshi Culture (Late Xia Dynasty to Early Shang). Yi-style Gui-pot in northern Shandong only excavated from burials. They existed with Shang-style Gui-pot in the same graveyard, but never together in the same burial. So Yi-style Gui-pot should have been treated as social identity goods. Another site where Yi-style Gui-pots existed is Yinxu in Anyang, the capital of late Shang period. Literature and oracle bone inscription reveal the relationship between Shang royal court and East-Yi people was quite close. Yi-style Gui-pots excavated from Yinxu site should be reflection of the relationship. However, although most Yi-style Gui-pots were existed in burials, several cases found in ash pits and cultural layers unexpectedly, which indicates that their functions had changed to a certain extent after Yi-style Gui-pots left their country of origin. Yi-style Gui-pot of late Shang and early-middle Zhou were also found in Zhouyuan site in Shaaxi, and even west to Gansu province. I believe they were remains of East-Yi people including Qin people and other groups, which were recorded in ancient documents.

Involvement of Orai1 in tunicamycin-induced endothelial dysfunction

Hui Yang,Yumei Xue,Sujuan Kuang,Mengzhen Zhang,Jinghui Chen,Lin Liu,Zhixin Shan,Qiuxiong Lin,Xiaohong Li,Min Yang,Hui Zhou,Fang Rao,Chunyu Deng 대한약리학회 2019 The Korean Journal of Physiology & Pharmacology Vol.23 No.2

Endoplasmic reticulum (ER) stress is mediated by disturbance of Ca2+ homeostasis. The store-operated calcium (SOC) channel is the primary Ca2+ channel in non-excitable cells, but its participation in agent-induced ER stress is not clear. In this study, the effects of tunicamycin on Ca2+ influx in human umbilical vein endothelial cells (HUVECs) were observed with the fluorescent probe Fluo-4 AM. The effect of tunicamycin on the expression of the unfolded protein response (UPR)-related proteins BiP and CHOP was assayed by western blotting with or without inhibition of Orai1. Tunicamycin induced endothelial dysfunction by activating ER stress. Orai1 expression and the influx of extracellular Ca2+ in HUVECs were both upregulated during ER stress. The SOC channel inhibitor SKF96365 reversed tunicamycin-induced endothelial cell dysfunction by inhibiting ER stress. Regulation of tunicamycin-induced ER stress by Orai1 indicates that modification of Orai1 activity may have therapeutic value for conditions with ER stress-induced endothelial dysfunction.