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( Jun Pei Zhou ),( Qian Wu ),( Rui Zhang ),( Yu Ying Yang ),( Xiang Hua Tang ),( Jun Jun Li ),( Jun Mei Ding ),( Yan Yan Dong ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.6
This paper reports the production and characterization of crude xylanase from the newly isolated Humicola sp. Ly01. The highest (41.8 U/ml) production of the crude xylanase was obtained under the optimized conditions (w/v): 0.5% wheat bran, 0.2% KH2PO4, and 0.5% peptone; initial pH 7.0; incubation time 72 h; 30℃; and 150 rpm. A considerable amount of the crude xylanase was induced using hulless barley bran or soybean meal as the carbon source, but a small amount of the enzyme was produced when supplementary urea was used as the nitrogen source to wheat bran. The crude xylanase showed apparent optimal cellulase-free xylanase activity at 60℃ and pH 6.0, more than 71.8% of the maximum xylanase activity in 3.0-30.0% (v/v) ethanol and more than 82.3% of the initial xylanase activity after incubation in 3.0-30.0% (v/v) ethanol at 30℃ for 2 h. The crude xylanase was moderately resistant to both acid and neutral protease digestion, and released 7.9 and 10.9 μmol/ml reducing sugar from xylan in the simulated gastric and intestinal fluids, respectively. The xylooligosaccharides were the main products of the hydrolysis of xylan by the crude xylanase. These properties suggested the potential of the crude enzyme for being applied in the animal feed industry, xylooligosaccharides production, and high-alcohol conditions such as ethanol production and brewing.
Jun Guan,Yu Xue,Rong-yu Zang,Ji-hong Liu,Jian-qing Zhu,Ying Zheng,Bo Wang,Hua-ying Wang,Xiao-jun Chen 대한부인종양학회 2021 Journal of Gynecologic Oncology Vol.32 No.4
Background: Sentinel lymph node (SLN) mapping has been recommended as an alternative staging approach to lymphadenectomy for apparent uterine-confined endometrial cancer (EC). However, the prognostic value of SLN mapping alone instead of systematic lymphadenectomy on EC patients remains unclear. Methods: A multi-center, open label, non-inferiority randomized controlled trial has been designed to identify if SLN mapping alone is not inferior to pelvic lymphadenectomy on prognosis of patients with intermediate-high-risk EC clinically confined to uterus. Eligible patients will be 1:1 randomly assigned to accept SLN mapping or pelvic lymphadenectomy. The primary endpoint is the 2-year progression-free survival (PFS). The second points are the 5-year PFS, 5-year overall survival, surgery-related adverse events and life quality. A total of 780 patients will be enrolled from 6 hospitals in China within 3-year period and followed up for 5 years. Trial Registration: ClinicalTrials.gov Identifier: NCT04276532
A fast IMDCT algorithm for MPEG-2 AAC decoder
Hua-Jun Chi,Tae-Hoon Kim,Koon-shik Cho,Ju-Sung Park 대한전자공학회 2007 ITC-CSCC :International Technical Conference on Ci Vol.2007 No.7
This paper proposes a new IFFT(Inverse Fast Fourier Transform) algorithm, which is proper for IMDCT((Inverse Modified Discrete Cosine Transform) of MPEG-2 AAC(Advanced Audio Coding) decoder. The IFFT used in 2<SUP>N</SUP>-point IMDCT employ the bit-reverse data arrangement of inputs and N/4-IFFT to reduce the calculation cycles. We devised a new data arrangement algorithm of IFFT input and N/4ⁿ?¹-IFFT and can reduce multiplication cycles, addition cycles, and ROM size.
THE U.S. TRADE DEFICIT WITH CHINA: CAUSES AND PROPOSED SOLUTIONS
Jun hua Jia,Suk Kim People&Global Business Association 2008 Global Business and Finance Review Vol.13 No.2
The U.S.-China trade relationship has undergone tremendous growth since 1979 when the United Stares and China establish their diplomatic relationship. The trade volumes have increased dramatically after China joined the World Trade Organization in 2001. Recently, the trade relationship between the two countries has experienced some setbacks-specifically in terms of the huge U.S. trade deficits with China. the currency manipulation by the Chinese government. and China's failure in enforcing strict laws to protect intellectual property rights (IPR) of U.S. companies. Effective solutions to address such trade frictions may involve consideration of trade policies. negotiation. and cooperation between the two countries. This article first provides an overview the current trade status and frictions between U.S. and China. Next, the possible root causes of such tensions are explored Finally. possible solutions are presented to alleviate such tensions and to allow both countries benefit from a mutually harmonious trade relationship.
Organization process of the hierarchical structures in microbially synthesized polyhydroxyalkanoates
Jun Xu,Bao-Hua Guo,Qiong Wu,Jin-Chun Chen,Guo-Qiang Chen,Jian-Jun Zhou,Yong Jiang,Lin Li 한국물리학회 2007 Current Applied Physics Vol.7 No.s1
cess of the high-order structures in biomaterials. Real-time Fourier transform infrared spectroscopy and optical microscopy demon-strated that helical segments formed along with the spherulite growth. Atomic force microscopy revealed the details of growth,twisting and branching of lamellar crystals. Cooperative packing of these twisting lamellae led to regular banded spherulites observedunder polarized light microscopy. Real-time observation on the crystallization process provided richer information than the characterization of the final structures; consequently, it provides deeper insight into the organization mechanism of the hierarchical structures.
Jun-yi Hua,Yu-zhou He,Yun Xu,Xu-hong Jiang,Wu Ye,Zhi-min Pan 생화학분자생물학회 2015 Experimental and molecular medicine Vol.47 No.-
Neointimal proliferation after vascular injury is a key mechanism of restenosis, a major cause of percutaneous transluminal angioplasty failure and artery bypass occlusion. Emodin, an anthraquinone with multiple physiological activities, has been reported to inhibit proliferation of vascular smooth muscle cells (VSMCs) that might cause intimal arterial thickening. Thus, in this study, we established a rat model of balloon-injured carotid artery and investigated the therapeutic effect of emodin and its underlying mechanism. Intimal thickness was analyzed by hematoxylin and eosin staining. Expression of Wnt4, dvl-1, β-catenin and collagen was determined by immunohistochemistry and/or western blotting. The proliferation of VSMC was evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and electron microscopy. MicroRNA levels were quantified by real-time quantitative PCR. Emodin relieved injury-induced artery intimal thickness. Results of western blots and immunohistochemistry showed that emodin suppressed expression of signaling molecules Wnt4/Dvl-1/β-catenin as well as collagen protein in the injured artery. In addition, emodin enhanced expression of an artery injury-related microRNA, miR-126. In vitro, MTT assay showed that emodin suppressed angiotensin II (AngII)-induced proliferation of VSMCs. Emodin reversed AngII-induced activation of Wnt4/Dvl-1/β-catenin signaling by increasing expression of miR-126 that was strongly supported by transfection of mimic or inhibitor for miR-126. Emodin prevents intimal thickening via Wnt4/Dvl-1/β-catenin signaling pathway mediated by miR-126 in balloon-injured carotid artery of rats.
( Jun Tao ),( Fa-liang An ),( Zheng-hua Pan ),( Yan-hua Lu ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.5
Biofilms are of vital significance in bioconversion and biotechnological processes. In this work, sugarcane molasses was used to enhance biofilms for the improvement of the production of fumigaclavine C (FC), a conidiation-associated ergot alkaloid with strong anti-inflammatory activities. Biofilm formation was more greatly induced by the addition of molasses than the addition of other reported biofilm inducers. With the optimal molasses concentration (400 g/l), the biofilm biomass was 6-fold higher than that with sucrose, and FC and conidia production was increased by 5.8- and 3.1-fold, respectively. Moreover, the global secondary metabolism regulatory gene laeA, FC biosynthetic gene fgaOx3, and asexual central regulatory genes brlA and wetA were upregulated in molasses-based biofilms, suggesting the upregulation of both asexual development and FC biosynthesis. This study provides novel insight into the stimulatory effects of molasses on biofilm formation and supports the widespread application of molasses as an inexpensive raw material and effective inducer for biofilm production.
An eigenspace projection clustering method for structural damage detection
Jun-hua Zhu,Ling Yu,Li-li Yu 국제구조공학회 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.44 No.2
An eigenspace projection clustering method is proposed for structural damage detection by combining projection algorithm and fuzzy clustering technique. The integrated procedure includes data selection, data normalization, projection, damage feature extraction, and clustering algorithm to structural damage assessment. The frequency response functions (FRFs) of the healthy and the damaged structure are used as initial data, median values of the projections are considered as damage features, and the fuzzy c-means (FCM) algorithm are used to categorize these features. The performance of the proposed method has been validated using a three-story frame structure built and tested by Los Alamos National Laboratory, USA. Two projection algorithms, namely principal component analysis (PCA) and kernel principal component analysis (KPCA), are compared for better extraction of damage features, further six kinds of distances adopted in FCM process are studied and discussed. The illustrated results reveal that the distance selection depends on the distribution of features. For the optimal choice of projections, it is recommended that the Cosine distance is used for the PCA while the Seuclidean distance and the Cityblock distance suitably used for the KPCA. The PCA method is recommended when a large amount of data need to be processed due to its higher correct decisions and less computational costs.