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Jing-Hua Tzeng,Chih-Huang Weng,Yu-Hao Lin,Shang-Ming Huang,Li-Ting Yen,Jin Anotai,Yao-Tung Lin 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-
A novel visible light driven tourmaline-nitrogen-doped-TiO2 composite (S-N-TiO2) was prepared using afacile impregnation and sol gel method and its photocatalytic reaction scheme with ethylene wasproposed. X-ray diffraction analysis confirmed the presence of TiO2 in the form of anatase phase. Scanning electron microscope and energy dispersive spectrometer mapping showed that the TiO2particles were deposited and dispersed on the surface of tourmaline. Under visible light irradiation, the SN-TiO2 catalyst containing 4 wt.% tourmaline has higher photocatalytic activity for the oxidation ofethylene than pure TiO2 and N-doped-TiO2 (N-TiO2). This enhanced activity could be not only attributedto the narrowed band gap in visible light driven N-TiO2, but also improved by the spontaneous electricfield of tourmaline which was applied to restrain the recombination of the electron–hole pairs. Thephotogenerated electrons from N-TiO2 were induced by electricfield to react with ethylene, and theleaving photogenerated holes also formed the reactive species. The photocatalytic activity of S-N-TiO2 ismuch affected by synthesis conditions. This novel S-N-TiO2 photocatalyst has a promising perspective inthe gas treatment for air pollution control and horticultural product industries.
Impairment of AMPK-α2 augments detrusor contractions in bladder ischemia
Jing-Hua Yang,Wanting Niu,Yedan Li,Kazem M. Azadzoi 대한비뇨의학회 2021 Investigative and Clinical Urology Vol.62 No.5
Purpose: Ischemia disrupts cellular energy homeostasis. Adenosine monophosphate-activated protein kinase alpha-2 (AMPK-α2) is a subunit of AMPK that senses cellular energy deprivation and signals metabolic stress. Our goal was to examine the expression levels and functional role of AMPK-α2 in bladder ischemia. Materials and Methods: Iliac artery atherosclerosis and bladder ischemia were engendered in apolipoprotein E knockout rats by partial arterial endothelial denudation using a balloon catheter. After eight weeks, total and phosphorylated AMPK-α2 expression was analyzed by western blotting. Structural integrity of AMPK-α2 protein was assessed by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Functional role of AMPK-α2 was examined by treating animals with the AMPK activator 5-aminoimidazole-4-carboxamide-1-beta-D ribofuranoside (AICAR). Tissue contractility was measured in the organ bath and bladder nerve density was examined by immunostaining. Results: Total AMPK-α2 expression increased in bladder ischemia, while phosphorylated AMPK-α2 was significantly downregulated. LC-MS/MS suggested post-translational modification of AMPK-α2 functional domains including phosphorylation sites, suggesting accumulation of catalytically inactive AMPK-α2 in bladder ischemia. Treatment of rats with AICAR diminished the force of overactive detrusor contractions and increased bladder capacity but did not have a significant effect on the frequency of bladder contractions. AICAR diminished contractile reactivity of ischemic tissues in the organ bath and prevented loss of nerve fibers in bladder ischemia. Conclusions: Ischemia induces post-translational modification of AMPK-α2 protein. Impairment of AMPK-α2 may contribute to overactive detrusor contractions and loss of nerve fibers in bladder ischemia. AMPK activators may have therapeutic potential against detrusor overactivity and neurodegeneration in bladder conditions involving ischemia.
Jing Si,Jian-hui Wang,Li-jing Zhang,Hua Zhang,Ya-jie Liu,Li-zhe An 한국식물학회 2009 Journal of Plant Biology Vol.52 No.6
Rapid amplification of cDNA ends was performed to isolate cold-regulated CbCOR15 (EF208112) from Chorispora bungeana. This alpine species is distributed in subnival areas. Transcripts were detected in the leaves, but not the roots, of plants acclimated to cold temperatures. Expression was induced at high levels at both 4°C and −4°C. In comparing its deduced protein sequence to that of AtCOR15a (cold-regulated 15a in Arabidopsis thaliana), the N terminus had less homology than the C terminus while still containing a region analogous to the chloroplast-targeted signal peptide of AtCOR15a. We also introduced CbCOR15, with the CaMV 35S promoter, into tobacco. Second-generation (T1) plants had significantly increased tolerance to chilling, as determined by their electrolyte leakage, chlorophyll content, and relative water content. Further freezing-stress experiments showed that the tolerance of transgenic lines was significantly greater than that of the nontransgenics. Although the degree of chilling and freezing tolerance in the transgenic plants was not directly correlated with the accumulated levels of CbCOR15, we could conclude that this gene confers cold tolerance.
Assessment of the Cytotoxic and Apoptotic Effects of Chaetominine in a Human Leukemia Cell Line
( Jing Yun Yao ),( Rui Hua Jiao ),( Chang Qing Liu ),( Yu Peng Zhang ),( Wan Guo Yu ),( Yan Hua Lu ),( Ren Xiang Tan ) 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.2
Chaetominine is a quinazoline alkaloid originating from the endophytic fungus Aspergillus fumigatus CY018. In this study, we showed evidence that chaetominine has cytotoxic and apoptotic effects on human leukemia K562 cells and investigated the pathway involved in chaetominine-induced apoptosis in detail. Chaetominine inhibited K562 cell growth, with an IC50 value of 35 nM, but showed little inhibitory effect on the growth of human peripheral blood mononuclear cells. The high apoptosis rates, morphological apoptotic features, and DNA fragmentation caused by chaetominine indicated that the cytotoxicity was partially caused by its pro-apoptotic effect. Under chaetominine treatment, the Bax/Bcl-2 ratio was upregulated (from 0.3 to 8), which was followed by a decrease in mitochondrial membrane potential, release of cytochrome c from mitochondria into the cytosol, and stimulation of Apaf-1. Furthermore, activation of caspase-9 and caspase-3, which are the main executers of the apoptotic process, was observed. These results demonstrated that chaetominine induced cell apoptosis via the mitochondrial pathway. Chaetominine inhibited K562 cell growth and induced apoptotic cell death through the intrinsic pathway, which suggests that chaetominine might be a promising therapeutic for leukemia.
Jing Hua,Zhipeng Lu,Hengbo Yin,Wuping Xue,Aili Wang,Lingqin Shen,Shuxin Liu 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.40 No.-
Aldol condensation of acetic acid with formaldehyde to acrylic acid was catalyzed by the SiO2-, SBA-15-,and HZSM-5-supported vanadium phosphorous oxide (V-P-O) catalysts prepared by the incipientwetness impregnation. The supports obviously increased the surface areas and changed the acid–baseproperties of the as-prepared catalysts. V-P-O/SBA-15 catalysts with high acid and alkali quantitiesexhibited high catalytic activities for the aldol condensation of acetic acid with formaldehyde to acrylicacid. When the V-P-O/SBA-15 catalyst with the P/V mole ratio of 2.0:1 catalyzed the reaction at 330–370 8C, the acrylic acid selectivities were 90.8–70.2% at the formaldehyde conversions of 14.3–68.7%.
NF-κB in Cellular Senescence and Cancer Treatment
Hua Jing,Soyoung Lee 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.3
The NF-κB pathway transcriptionally controls a large set of target genes that play important roles in cell survival, inflammation, and immune responses. While many studies showed anti-tumorigenic and pro-survival role of NF-κB in cancer cells, recent findings postulate that NF-κB participates in a senescence-associated cytokine response, thereby suggesting a tumor restraining role of NF-κB. In this review, we discuss implications of the NF-κB signaling pathway in cancer. Particularly, we emphasize the connection of NF-κB with cellular senescence as a response to chemotherapy, and furthermore, present examples how distinct oncogenic network contexts surrounding NF-κB produce fundamentally different treatment outcomes in aggressive B-cell lymphomas as an example.
NF-κB in Cellular Senescence and Cancer Treatment
Jing, Hua,Lee, Soyoung Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.3
The NF-${\kappa}B$ pathway transcriptionally controls a large set of target genes that play important roles in cell survival, inflammation, and immune responses. While many studies showed anti-tumorigenic and pro-survival role of NF-${\kappa}B$ in cancer cells, recent findings postulate that NF-${\kappa}B$ participates in a senescence-associated cytokine response, thereby suggesting a tumor restraining role of NF-${\kappa}B$. In this review, we discuss implications of the NF-${\kappa}B$ signaling pathway in cancer. Particularly, we emphasize the connection of NF-${\kappa}B$ with cellular senescence as a response to chemotherapy, and furthermore, present examples how distinct oncogenic network contexts surrounding NF-${\kappa}B$ produce fundamentally different treatment outcomes in aggressive B-cell lymphomas as an example.