토끼 간조직의 Stimulatory Guanine Nucleotide 결합 단백의 αsubunit 에 대한 cDNA 염기서열에 관한 연구

이상훈,노연희,한중수,고재경 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

G proteins, a family of guanine nucleotide-binding protein superfamily, are involved in transmembrane signaling systems as transducers. In this study cDNAs encoding αsubunit of the stimulatory G protein (G α ) from rabbit liver were cloned and their nucleotide sequences were determined : a λgt 11 cDNA library from total cellular poly (A)' mRNA in rabbit liber was screened for recombinant λDNA that codes for G α with labeled probe. The probe used was an EcoRI digested 3' end fragment of cDNA for G α from olfactory neuroepithelium of rat. Six of the approximately recombinant clones were screened by in situ hybridization and by autoradiography, detecting sixteen positive clones. These clones were tested using polymerase chain reaction (PCR) technique and thirteen of them were turned out to have two sets of conserved sequence in αcDNA. Four clonse among them were selected form analysis of their cDNA sequences, showing the presence of two types of cDNA, namely and with the total length of nucleotide sequence of1.392bp and 1.613bp, respectively . From nucleotide sequence analysis the amino acid residues of -1 and α-2 were deduced : they contain 335 and 386 amino acid residues (including the initiator methionine), respectively. The calculated molecular weights for and were 38.9kd and 44.6kd, respectively. The significant difference in size between two cDNAs for G αseemed to be due to the assumption that alternative promoter and leading exon might be involved in transcribing the mRNA for . Two types of cDNA from rabbit liver shared over 95% of amino acid homology with that from rat olfactory neuroepichelium if deleted portions are not counted. More information on the cDNAs could be obtained through further studies such as Sl nuclease protection experiment, expression and mutation study.

沖積粘土의 物理的性質에 關한 硏究

朴在云,金振勳 관동대학교 1992 關大論文集 Vol.20 No.2

This study was made to find several significant relations among varous physical properties. The soil samples ware taken at district of Korean area, where the structures would be placed on the alluvial clay foundation. It was the statistical analysis through computer programs, Minitab statistics package, were carried out using the result of laboratory tests of 465 soil damples. The results ofthe study are summarized as follows; 1. Judging form Casagrande's plasticity chart, it is considered that almost all soil samples belong to inorganic clay with medium or high plasticity. 2. It is certainthat the foundation is weak because the natural moisture contents are much higher then the liquid limits. 3. When the initial void ratio whtch playscrucial role to the settlement of foundation is more than 2.5, the compression indices of soil samples are remakably scattered, but these indices, which ar formulated as a general expression, tend to increasing the clay content, natural moisture content, initial void ratio, liquid limit and plasticity index.

韓國人의 人乳組成에 관한 연구

오훈,이재영 成均館大學校 科學技術硏究所 1988 論文集 Vol.39 No.2

This experiment was carried out to compare chemical compositions, mineral contents, amino acid compositions and electrophoretic patterns of protein of Korean human and cow's milk. The results are summarized as follows: 1. Human milk appeared to contain similar amount of total solids (11.28%) and fat(3.1%) to those of cow's milk, but higher lactose(6.77%) content and lower in protein(1.27%) and ash(0.19%) contents. 2. Ratios of Calcium to Phosphorus in human and cow's milk were 2. 63 : 1 and 1. 27 : 1, respectively, indicating ratio of calcium to Phosphorus in human milk was higher than that of cow's milk. Generally, major mineral contents in human milk was lower than that of cow's milk. 3. Ratios of casein to whey protein in human and cow's mills were 1 : 1.14 and 1 : 0. 2, respectively, indicating proportion of whey protein in human milk was higher than that of cow's milk. Content of nor-protein-nitrogen of human milk appeared to be 20.67%, which was 4 times as high as that of cow's milk. 4. Analytical results of amino acid compositions of human milk indicated that amino acid contents in human milk were lower than those of cow's milk as a whole, but cys and phe contents is human milk were higher than those of cow's milk. 5. Results from Slab-gel electrophoretic patterns of human milk casein showed that β -casein was a major component consisting of six bands and α_s1-casein which is one of the major component of cow's milk casein was absent. 6. Of human whey protein, lactalbumin appeared to be a major component. And other proteins such as lactoferrin, immuno-globulin and lysozyme formed a spearding zone altogether on the slab-gel. 7. β-Lactoglobulin which is one of the major components in cow's whey protein was absent in whey protein of human milk.

골프 연습장의 서비스 품질 영향 요인과 개선 방안에 대한 연구

이훈석,조재립 경희대학교 레이저공학연구소 2000 레이저공학 Vol.11 No.-

The golf industry is growing fast with increasing leisure time and income, and changing preference of leisure sports. Therefore, to development golf industry, it is time to make a managing alternative based on service industry, understanding consumer satisfaction factors, and overcoming competition among competitors. As preceding study for the overall golf industry, this study is focused on the golf training center service. The purpose of this study is to get a practical and theoretical suggestion by examining closely how the service quality can effect the relationship between the service quality factors perceived by customers and the level of the satisfaction by customers, focusing on the service offered by the golf training center. This paper was based on data which were collected from assessment of customers who visited the golf training center with self-administered structured questionnaire. In order to accumulate the data necessary for this study, the collected data is run by STATISTICA,

서비스품질 결정요인(CTQ)분석 및 고객만족 향상에 관한 연구

김경훈,조재립 경희대학교 산학협력기술연구원 2002 산학협력기술연구논문집 Vol.8 No.-

Now we live in service society. The gravity of service industry grows high and the importance of service industry is increasing continuously. Accordingly it is essential that we offer service satisfying customer's desire and improvement service quality by grasping critical factor form management successfully of service quality. Customer satisfaction will improve by making an effort on improvement critical success factor f service quality. This study is to determine customer's critical factor using AHP(Analytic Hierarchy Process) technique and to analyze customer's needs in the service of department store. This study's object is that it selects the critical factor influencing and management item of service quality using QFD(Quality Function Depolyment) technique about this critical factor and improves customer satisfaction through improvement of service quality for providing better service in the department store.

증권거래 서비스품질 평가항목 개발에 관한 연구

이훈석,조재립 경희대학교 산학협력기술연구원 1999 산학협력기술연구논문집 Vol.5 No.-

난소성 장액성 낭선암에 특이한 단백의 분리에 관한 연구

이상훈,장상근,고재경,김두상 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.1

In order to correlate changes in the nature of proteins in the serous cystadenocarcinoma tissue of the ovary with those in the cystic fluid and ascitic fluid of patients with the ovarian cancer, proteins in the ovarian tissue, cystic fluid and ascitic fluid of patients with serous cystadenocarcinoma were isolated by a DEAE-cellulose column chromatography and HPLC. (1)Concentrations of protein in the ovarian cancer tissue, cystic fluid and ascitic fluid of patients with serous cystadenocarcinoma were unchanged as compared with those of controls. (2)Proteins in the ovarian tissues were isolated by a DEAE-cellulose column chromatography into 7 peaks in both serous cystadenocarcinoma tissue and control ovarian tissue. The DEAE-peak Ⅲ protein was divided into two parts (peak ?? and ??) in the ovarian cancer, but not in the control, indicating that the DEAE-peak Ⅲ?? was specific to the ovarian cancer. The DEAE-peak Ⅴ common to both serous cystadenocarcinoma tissue and its control was further separated by a HPLC into 4 peaks each. Although the HPLC patterns for proteins of both tissues appeared to be different, presence of proteins specific to the ovarian cancer was not confirmed in the ovarian cancer tissue. (3)Proteins in the ovarian cystic fluid were isolated by a DEAE-cellulose column chromatography into 5 peaks for serous cystadenocarcinoma and 4 peaks for serous cyst as the control of which the peak Ⅳ protein was divided into two parts(peak ?? and ??) in the ovarian cancer, but not in the control. Two protein peaks (DEAE-peak ?? and ??) in the ovarian cancer, but not control. Two protein peaks (DEAE-peak ?? and Ⅷ) were observed to be specific to the ovarian cancer. (4)Proteins in the ascitic fluid were separated by a DEAE-cellulose column chromatography into 7 peaks for the ovarian cancer and 5 peaks for the serous cyst, of which the peak Ⅳ protein was also divided into two parts as in the cystic fluid of the ovarian cancer, but not in the control. Three protein peaks (peak Ⅲ, ?? and Ⅷ) appeared to be specific to the ovarian cancer. These results indicated that concentrations of proteins were unchanged, but the nature of proteins were changed in the cancer tissue, cystic fluid and ascitic fluid of patients with serous cystadenocarcinoma and that proteins specific to the ovarian cancer were present in at least one for the ovarian cancer tissue, two for the cystic fluid and three for the ascitic fluid. The proteins specific to serous cystadenocarcinoma in the cystic and ascitic fluid appeared to be released from the ovarian cancer tissue and could be used as biochemical markers for the cancer.

석재복합제품 관련산업의 신제품 개발전략

김재명,손재영,최용훈,오정화 강원대학교 석재복합신소재제품연구센터 1998 석재연 논문집 Vol.3 No.-

본 연구는 신제품 개발이론에 대한 이론적 틀을 연구하고, 이에 기초하여 석재복합 관련제품의 신제품 개발 노선, 기술, 신제품 개발전략 구축과 선택 방안을 제시하였다. 석재복합 관련 제품의 대부분은 첨단 고도기술을 필요로 하는 영역이 한정되어 있기 때문에 이 산업 분야에서의 신제품 개발은 신제품 개발계획과 지침을 마련할 필요가 있다. 또한 유망 신제품 안건으로 계층화하여 신제품 개발의 기본전략을 구축하는 신규사업 시나리오를 작성하여 신제품 개발전략을 구축하는 것이 효과적이다. 이러한 과정을 거쳐 중소기업의 경우는 현존하는 시장, 제품, 그리고 기술우위에 기반을 둔 신제품을 개발할 경우 성공 가능성이 클 것이다. 이 이외에도 석재복합제품 개발은 석재자원과 신소재 개발에 기초한 신제품 개발에 필요한 기술 진전 사항, 즉 기술개발-제품개발-시장진입 활동간 상호 연계성을 고려해야 한다. This study suggests the theoretical framework and strategy of new product development in the avdanced mineral aggregate composite industry. The advanced mineral aggregate composite area needs to prepare the plan and guidelines on the new product development owing to the characteristic of the related technology. Alternative scenarios on the hierachy of new product idea is effective to the success of new product development strategy. Especially, the existing market, product, and the technological advantages affects the possibility of the success of new product development of the small firms. Also considering the interrelationship between the technological trends is important to the new product development of advanced mineral aggregate composite industry.

모험기업의 라이프 싸이클 단계별 재무전략

김재명,오정화,손재영,최용훈 강원대학교 석재복합신소재제품연구센터 1997 석재연 논문집 Vol.2 No.-

본 논문은 신제품의 사업화와 관련한 모험기업의 라이프 싸이클 단계별 재무전략, 특히 자금조달 전략에 관해 연구하였다. 특히 금융상품의 유형과 특성을 자본원천별로 분석하고, 이에 기초하여 모험기업의 성장단계별 자금조달전략을 제시하였다. 본 논문은 라이프싸이클을 4단계로 나누고, 각 단계별 재무특성과 자금원천의 특성을 고려하여 라이프싸이클 단계별 자금조달원천 행렬표를 제시하였다. 이에 따르면 모험기업은 라이프싸이클 단계별로 개인저축, 에인젤, 모험자본, 동업자, 기업공개, 그리고 잉여금 등의 지분자본원천과 회사채, 거래신용, 자산기초금융, 은행이나 보험회사 차입, 그리고 정책지원자금 등 부채자본원천을 중첩하여 자본조달원천으로 사용할 수 있음을 보여주고 있다. This paper discusses the relationships between stage of development in venture business life cycles and financial strategies. The paper selectively examines the study by reviewing the characteristics of the various finacial instruments on the basis of the cash flows of venture business life cycles. I suggested the source of capital matrix combining the various sources of capital. At each stage, the venture businessess should raise the funds by using the various combinations of sources of equity financing and debt financing.

X-ray 조사에 의한 제주산 감자 저장

김재하,강훈 濟州大學校 工科大學 産業技術硏究所 1993 産業技術硏究報告 Vol.4 No.-

For extension of storage with Cheju grown potatoes, irradiations with doses of 5 Krad. 10 Krad and 15 Krad were applied and the differences of quality were compared between the irradiated and the unirradiated and also between doses. Irradiation is not effective on the prevention of decaying. Irradiation of 5 Krad is too low compare with 10 Krad and 15 Krad and 10 Krad is more effective and energy saving than 15 Krad. Among same irradiation doses, outdoor storage showed better quality than those stored in enclosed space in the room.